LOCUS       BBa_J58119              1926 bp    DNA     linear       22-Nov-2024
DEFINITION  Promoter OmpR modified + inverter TetR + lambda cI generator
ACCESSION   BBa_J58119
VERSION     BBa_J58119.1  
FEATURES             Location/Qualifiers
     misc_feature    13..30
                     /label=C1 OmpR
     misc_feature    37..44
                     /label=linker
     promoter        45..50
                     /label=-35
     promoter        68..73
                     /label=-10
     misc_feature    41..41
                     /label=A
     promoter        1..78
                     /label=BBa_R0083
     misc_feature    91..94
                     /label=conserved
     RBS             87..98
                     /label=BBa_B0034
     CDS             108..724
                     /label=tetR
     misc_feature    725..758
                     /label=SsrA
     misc_feature    765..789
                     /label=Help:Barcodes
     CDS             105..764
                     /label=BBa_C0040
     stem_loop       809..852
                     /label=stem_loop
     misc_feature    798..877
                     /label=BBa_B0010
     terminator      798..877
                     /label=BBa_B0010
     stem_loop       893..912
                     /label=T7 TE
     misc_feature    919..919
                     /label=stop
     polyA_site      913..926
                     /label=polya
     misc_feature    886..926
                     /label=BBa_B0012
     terminator      886..926
                     /label=BBa_B0012
     misc_feature    935..953
                     /label=TetR 1
     misc_feature    935..988
                     /label=BBa_R0040
     promoter        954..959
                     /label=-35
     misc_feature    960..978
                     /label=TetR 2
     promoter        977..982
                     /label=-10
     promoter        935..988
                     /label=BBa_R0040
     misc_feature    87..988
                     /label=BBa_Q04400
     misc_feature    1..988
                     /label=BBa_J58117
     misc_feature    1001..1004
                     /label=conserved
     RBS             997..1008
                     /label=BBa_B0034
     CDS             1018..1725
                     /label=cI lambda
     misc_feature    1726..1758
                     /label=LVA
     misc_feature    1765..1789
                     /label=Help:Barcodes
     CDS             1015..1764
                     /label=BBa_C0051
     stem_loop       1809..1852
                     /label=stem_loop
     misc_feature    1798..1877
                     /label=BBa_B0010
     terminator      1798..1877
                     /label=BBa_B0010
     stem_loop       1893..1912
                     /label=T7 TE
     misc_feature    1919..1919
                     /label=stop
     polyA_site      1913..1926
                     /label=polya
     misc_feature    1886..1926
                     /label=BBa_B0012
     terminator      1886..1926
                     /label=BBa_B0012
     misc_feature    997..1926
                     /label=BBa_P0451
ORIGIN
        1 tcccttgcat ttacattttg aaacatctat agcgatctct tgagttggat tattctgcat
       61 ttttggggag aatggactta ctagagaaag aggagaaata ctagatgtcc agattagata
      121 aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa
      181 caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa
      241 aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc
      301 acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt
      361 ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta
      421 cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt
      481 cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat
      541 tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta
      601 tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct
      661 tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg
      721 ggtccgctgc aaacgacgaa aactacgctt tagtagctta ataacactga tagtgctagt
      781 gtagatcact actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
      841 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
      901 ttcgggtggg cctttctgcg tttatatact agagtcccta tcagtgatag agattgacat
      961 ccctatcagt gatagagata ctgagcacta ctagagaaag aggagaaata ctagatgagc
     1021 acaaaaaaga aaccattaac acaagagcag cttgaggacg cacgtcgcct taaagcaatt
     1081 tatgaaaaaa agaaaaatga acttggctta tcccaggaat ctgtcgcaga caagatgggg
     1141 atggggcagt caggcgttgg tgctttattt aatggcatca atgcattaaa tgcttataac
     1201 gccgcattgc ttgcaaaaat tctcaaagtt agcgttgaag aatttagccc ttcaatcgcc
     1261 agagaaatct acgagatgta tgaagcggtt agtatgcagc cgtcacttag aagtgagtat
     1321 gagtaccctg ttttttctca tgttcaggca gggatgttct cacctgagct tagaaccttt
     1381 accaaaggtg atgcggagag atgggtaagc acaaccaaaa aagccagtga ttctgcattc
     1441 tggcttgagg ttgaaggtaa ttccatgacc gcaccaacag gctccaagcc aagctttcct
     1501 gacggaatgt taattctcgt tgaccctgag caggctgttg agccaggtga tttctgcata
     1561 gccagacttg ggggtgatga gtttaccttc aagaaactga tcagggatag cggtcaggtg
     1621 tttttacaac cactaaaccc acagtaccca atgatcccat gcaatgagag ttgttccgtt
     1681 gtggggaaag ttatcgctag tcagtggcct gaagagacgt ttggcgctgc aaacgacgaa
     1741 aactacgctt tagtagctta ataacgctga tagtgctagt gtagatcgct actagagcca
     1801 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     1861 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     1921 tttata
//