LOCUS BBa_J58121 4790 bp DNA linear 22-Dec-2024 DEFINITION Detector of OmpR-P concentrations ACCESSION BBa_J58121 VERSION BBa_J58121.1 FEATURES Location/Qualifiers misc_feature 13..30 /label=C1 OmpR misc_feature 34..51 /label=C2 OmpR misc_feature 54..71 /label=C3 OmpR promoter 75..80 /label=-35 promoter 98..103 /label=-10 promoter 1..108 /label=BBa_R0082 misc_feature 121..124 /label=conserved RBS 117..128 /label=BBa_B0034 CDS 135..767 /label=BBa_J58112 stem_loop 787..830 /label=stem_loop misc_feature 776..855 /label=BBa_B0010 terminator 776..855 /label=BBa_B0010 stem_loop 871..890 /label=T7 TE misc_feature 897..897 /label=stop polyA_site 891..904 /label=polya misc_feature 864..904 /label=BBa_B0012 terminator 864..904 /label=BBa_B0012 misc_feature 1..904 /label=BBa_J58116 misc_feature 925..942 /label=C1 OmpR misc_feature 949..956 /label=linker promoter 957..962 /label=-35 promoter 980..985 /label=-10 misc_feature 953..953 /label=A promoter 913..990 /label=BBa_R0083 misc_feature 1003..1006 /label=conserved RBS 999..1010 /label=BBa_B0034 CDS 1020..1636 /label=tetR misc_feature 1637..1670 /label=SsrA misc_feature 1677..1701 /label=Help:Barcodes CDS 1017..1676 /label=BBa_C0040 stem_loop 1721..1764 /label=stem_loop misc_feature 1710..1789 /label=BBa_B0010 terminator 1710..1789 /label=BBa_B0010 stem_loop 1805..1824 /label=T7 TE misc_feature 1831..1831 /label=stop polyA_site 1825..1838 /label=polya misc_feature 1798..1838 /label=BBa_B0012 terminator 1798..1838 /label=BBa_B0012 misc_feature 1847..1865 /label=TetR 1 misc_feature 1847..1900 /label=BBa_R0040 promoter 1866..1871 /label=-35 misc_feature 1872..1890 /label=TetR 2 promoter 1889..1894 /label=-10 promoter 1847..1900 /label=BBa_R0040 misc_feature 999..1900 /label=BBa_Q04400 misc_feature 913..1900 /label=BBa_J58117 misc_feature 1913..1916 /label=conserved RBS 1909..1920 /label=BBa_B0034 CDS 1930..2637 /label=cI lambda misc_feature 2638..2670 /label=LVA misc_feature 2677..2701 /label=Help:Barcodes CDS 1927..2676 /label=BBa_C0051 stem_loop 2721..2764 /label=stem_loop misc_feature 2710..2789 /label=BBa_B0010 terminator 2710..2789 /label=BBa_B0010 stem_loop 2805..2824 /label=T7 TE misc_feature 2831..2831 /label=stop polyA_site 2825..2838 /label=polya misc_feature 2798..2838 /label=BBa_B0012 terminator 2798..2838 /label=BBa_B0012 misc_feature 1909..2838 /label=BBa_P0451 misc_feature 913..2838 /label=BBa_J58119 misc_feature 2849..2870 /label=CRP binding site misc_feature 2903..2920 /label=lam-cI OR2 binding site misc_feature 2871..2895 /label=spacer from I12007 (1-11, 59-72) promoter 2918..2923 /label=-35 promoter 2941..2946 /label=-10 misc_feature 2896..2902 /label=spacer from I12007 (26-32) misc_feature 2924..2940 /label=spacer from I12007 (54-70) misc_feature 2947..2952 /label=spacer from I12007 (77-82) promoter 2847..2952 /label=BBa_J58100 misc_feature 2965..2968 /label=conserved RBS 2961..2972 /label=BBa_B0034 CDS 2979..3653 /label=mrfp1 misc_feature 3660..3684 /label=Help:Barcodes CDS 2979..3659 /label=BBa_E1010 stem_loop 3704..3747 /label=stem_loop misc_feature 3693..3772 /label=BBa_B0010 terminator 3693..3772 /label=BBa_B0010 stem_loop 3788..3807 /label=T7 TE misc_feature 3814..3814 /label=stop polyA_site 3808..3821 /label=polya misc_feature 3781..3821 /label=BBa_B0012 terminator 3781..3821 /label=BBa_B0012 misc_feature 2961..3821 /label=BBa_I13507 misc_feature 2847..3821 /label=BBa_J58101 misc_feature 1..3821 /label=BBa_J58120 misc_feature 3842..3859 /label=C1 OmpR misc_feature 3866..3873 /label=linker promoter 3874..3879 /label=-35 promoter 3897..3902 /label=-10 misc_feature 3870..3870 /label=A promoter 3830..3907 /label=BBa_R0083 misc_feature 3920..3923 /label=conserved RBS 3916..3927 /label=BBa_B0034 CDS 3934..4653 /label=GFP protein CDS 3934..4653 /label=BBa_E0040 stem_loop 4673..4716 /label=stem_loop misc_feature 4662..4741 /label=BBa_B0010 terminator 4662..4741 /label=BBa_B0010 stem_loop 4757..4776 /label=T7 TE misc_feature 4783..4783 /label=stop polyA_site 4777..4790 /label=polya misc_feature 4750..4790 /label=BBa_B0012 terminator 4750..4790 /label=BBa_B0012 misc_feature 3830..4790 /label=BBa_J58103 ORIGIN 1 tcccttgcat ttacattttg aaacatctat agcgataaat gaaacatctt aaaagtttta 61 gtatcatatt cgtgttggat tattctgcat ttttggggag aatggactta ctagagaaag 121 aggagaaata ctagatggtg cttggcaaac cgcaaacaga cccgactctc gaatggttct 181 tgtctcattg ccacattcat aagtacccat ccaagagcac gcttattcac cagggtgaaa 241 aagcggaaac gctgtactac atcgttaaag gctctgtggc agtgctgatc aaagacgaag 301 agggtaaaga aatgatcctc tcctatctga atcagggtga ttttattggc gaactgggcc 361 tgtttgaaga gggccaggaa cgtagcgcat gggtacgtgc gaaaaccgcc tgtgaagtgg 421 ctgaaatttc gtacaaaaaa tttcgccaat tgattcaggt aaacccggac attctgatgc 481 gtttgtctgc acagatggcg cgtcgtctgc aagtcacttc agagaaagtg ggcaacctgg 541 cgttcctcga cgtgacgggc cgcattgcac agactctgct gaatctggca aaacaaccag 601 acgctatgac tcacccggac ggtatgcaaa tcaaaattac ccgtcaggaa attggtcaga 661 ttgtcggctg ttctcgtgaa accgtgggac gcattctgaa gatgctggaa gatcagaacc 721 tgatctccgc acacggtaaa accatcgtcg tttacggcac tcgttaatac tagagccagg 781 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 841 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 901 tatatactag agtcccttgc atttacattt tgaaacatct atagcgatct cttgagttgg 961 attattctgc atttttgggg agaatggact tactagagaa agaggagaaa tactagatgt 1021 ccagattaga taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa 1081 tcgaaggttt aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt 1141 attggcatgt aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata 1201 ggcaccatac tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata 1261 acgctaaaag ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag 1321 gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc 1381 aacaaggttt ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt 1441 taggttgcgt attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta 1501 ctactgatag tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg 1561 cagagccagc cttcttattc ggccttgaat tgatcatatg cggattagaa aaacaactta 1621 aatgtgaaag tgggtccgct gcaaacgacg aaaactacgc tttagtagct taataacact 1681 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 1741 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 1801 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtccc tatcagtgat 1861 agagattgac atccctatca gtgatagaga tactgagcac tactagagaa agaggagaaa 1921 tactagatga gcacaaaaaa gaaaccatta acacaagagc agcttgagga cgcacgtcgc 1981 cttaaagcaa tttatgaaaa aaagaaaaat gaacttggct tatcccagga atctgtcgca 2041 gacaagatgg ggatggggca gtcaggcgtt ggtgctttat ttaatggcat caatgcatta 2101 aatgcttata acgccgcatt gcttgcaaaa attctcaaag ttagcgttga agaatttagc 2161 ccttcaatcg ccagagaaat ctacgagatg tatgaagcgg ttagtatgca gccgtcactt 2221 agaagtgagt atgagtaccc tgttttttct catgttcagg cagggatgtt ctcacctgag 2281 cttagaacct ttaccaaagg tgatgcggag agatgggtaa gcacaaccaa aaaagccagt 2341 gattctgcat tctggcttga ggttgaaggt aattccatga ccgcaccaac aggctccaag 2401 ccaagctttc ctgacggaat gttaattctc gttgaccctg agcaggctgt tgagccaggt 2461 gatttctgca tagccagact tgggggtgat gagtttacct tcaagaaact gatcagggat 2521 agcggtcagg tgtttttaca accactaaac ccacagtacc caatgatccc atgcaatgag 2581 agttgttccg ttgtggggaa agttatcgct agtcagtggc ctgaagagac gtttggcgct 2641 gcaaacgacg aaaactacgc tttagtagct taataacgct gatagtgcta gtgtagatcg 2701 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 2761 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 2821 ggcctttctg cgtttatata ctagagagaa atgtgatcta gatcacattt gcaaccatta 2881 taaatagtgg tgataaaata gtcaacacgc acggtgttag atatttataa atagtggtga 2941 tagatttaac gttactagag aaagaggaga aatactagat ggcttcctcc gaagacgtta 3001 tcaaagagtt catgcgtttc aaagttcgta tggaaggttc cgttaacggt cacgagttcg 3061 aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag 3121 ttaccaaagg tggtccgctg ccgttcgctt gggacatcct gtccccgcag ttccagtacg 3181 gttccaaagc ttacgttaaa cacccggctg acatcccgga ctacctgaaa ctgtccttcc 3241 cggaaggttt caaatgggaa cgtgttatga acttcgaaga cggtggtgtt gttaccgtta 3301 cccaggactc ctccctgcaa gacggtgagt tcatctacaa agttaaactg cgtggtacca 3361 acttcccgtc cgacggtccg gttatgcaga aaaaaaccat gggttgggaa gcttccaccg 3421 aacgtatgta cccggaagac ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga 3481 aagacggtgg tcactacgac gctgaagtta aaaccaccta catggctaaa aaaccggttc 3541 agctgccggg tgcttacaaa accgacatca aactggacat cacctcccac aacgaagact 3601 acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt gcttaataac 3661 gctgatagtg ctagtgtaga tcgctactag agccaggcat caaataaaac gaaaggctca 3721 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 3781 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagt cccttgcatt 3841 tacattttga aacatctata gcgatctctt gagttggatt attctgcatt tttggggaga 3901 atggacttac tagagaaaga ggagaaatac tagatgcgta aaggagaaga acttttcact 3961 ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc 4021 agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact 4081 actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa 4141 tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc 4201 gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt 4261 gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat 4321 tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat 4381 gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac 4441 aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc 4501 gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa 4561 gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt 4621 acacatggca tggatgaact atacaaataa taatactaga gccaggcatc aaataaaacg 4681 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 4741 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata //