LOCUS BBa_J58122 3110 bp DNA linear 22-Nov-2024 DEFINITION Complete vanillin cellular receptor ACCESSION BBa_J58122 VERSION BBa_J58122.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 CDS 227..1117 /label=BBa_J58105 stem_loop 1137..1180 /label=stem_loop misc_feature 1126..1205 /label=BBa_B0010 terminator 1126..1205 /label=BBa_B0010 stem_loop 1221..1240 /label=T7 TE misc_feature 1247..1247 /label=stop polyA_site 1241..1254 /label=polya misc_feature 1214..1254 /label=BBa_B0012 terminator 1214..1254 /label=BBa_B0012 CDS 1263..1350 /label=end of LacI coding region (inactive) misc_feature 1263..1462 /label=BBa_R0010 misc_feature 1351..1388 /label=CAP binding site promoter 1399..1404 /label=-35 promoter 1423..1428 /label=-10 misc_feature 1428..1462 /label=LacI binding site misc_feature 1435..1435 /label=start promoter 1263..1462 /label=BBa_R0010 misc_feature 1475..1478 /label=conserved RBS 1471..1482 /label=BBa_B0034 CDS 1489..2973 /label=BBa_J58104 stem_loop 2993..3036 /label=stem_loop misc_feature 2982..3061 /label=BBa_B0010 terminator 2982..3061 /label=BBa_B0010 stem_loop 3077..3096 /label=T7 TE misc_feature 3103..3103 /label=stop polyA_site 3097..3110 /label=polya misc_feature 3070..3110 /label=BBa_B0012 terminator 3070..3110 /label=BBa_B0012 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga acatgaaaaa 241 actggctacc ctggtttccg ctgttgcgct aagcgccacc gtcagtgcga atgcgatggc 301 aaaagacacc atcgcgctgg tggtcgaaac gcttaacaaa ccggacaacg tatcgctgaa 361 agatggcgcg cagaaagagg cggataaact tggctataac ctggtggtgc tggactccca 421 gaacaacccg gcgaaagagc tggcgaacgt gcaggactta accgttcgcg gcacaaaaat 481 tctgctgatt gttccgaccg actccgacgc agtgggtaat gctgtgaaga tggctaacca 541 ggcgaacatc ccggttatca ctcttaaacg ccaggcaacg aaaggtgaag tggtgagcca 601 cattgctgcg gataacgtac tgggcggcaa aatcgctggt gattacatcg cgaagaaagc 661 gggtgaaggt gccaaagtta tcgagctgca aggcaaagct ggtacatccg cagcccgtga 721 actgggcgaa ggcttccagc aggccgttgc tgctcacaag tttaatgttc ttgccagcca 781 gccagcagat gaagatcgca ttaaaggttt gaacgtaatg cagaacctgt tgaccgctca 841 tccggatgtt caggctgtat tcgcgcagca ggatgaaatg gcgctgggcg cgctgcgcgc 901 actgcaaact gccggtaaat cggatgtgat ggtcgtcgga gacgttggta caccggatgg 961 cgaaaaagcg gtgaatgatg gcaaactagc agcgactatc gctgaactac ccgatcagat 1021 tggcgcgaaa ggcgtcgaaa ccgcagataa agtgctgaaa ggcgagaaag ttcaggctaa 1081 gtatccggtt gatctgaaac tggttgttaa gcagtagtac tagagccagg catcaaataa 1141 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg 1201 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag 1261 agcaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga 1321 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac 1381 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt 1441 gagcggataa caatttcaca catactagag aaagaggaga aatactagat gaatacaact 1501 ccctcacagc gattaggttt tttgcatcac atcaggttgg ttccgttatt tgcctgcatt 1561 ctaggcggta tcttagttct attcgcatta agttcagccc tggctggcta tttcctctgg 1621 caggccgatc gcgatcagcg tgatgttact gcggagattg agattcggac cgggttagcg 1681 aacagttcag attttttgcg ttcagcccgg atcaatatga ttcaggccgg ggctgcgagt 1741 cgtattgcgg aaatggaagc aatgaagcga aatattgcgc aagccgaatc ggagattaaa 1801 cagtcgcagc aaggttatcg tgcttatcag aatcgaccgg tgaaaacacc tgctgatgaa 1861 gccctcgaca ctgaattaaa tcaacgcttt caggcttata tcacgggtat gcaacctatg 1921 ttgaaatatg ccaaaaatgg catgtttgaa gcgattatca atcatgaaag tgagcagatc 1981 cgaccgctgg ataatgctta taccgatatt ttgaacaaag ccgttaagat acgtagcacc 2041 agagccaacc aactggcgga actggcccat cagcgcaccc gcctgggtgg gatgttcatg 2101 attggcgcgt ttgtgcttgc cctggtcatg acgctgataa catttatggt gctacgtcgg 2161 atcgtcattc gtccactgca acatgccgca caacggattg aaaaaatcgc cagtggcgat 2221 ctgacgatga atgatgaacc ggcgggtcgt aatgaaatcg gtcgcttaag tcgtcattta 2281 cagcatatgg cggctggtgt taagcaactg gcggatgacc gcacgctgct gatggcgggg 2341 gtaagtcacg acttgcgcac gccgctgacg cgtattcgcc tggcgactga gatgatgagc 2401 gagcaggatg gctatctggc agaatcgatc aataaagata tcgaagagtg caacgccatc 2461 attgagcagt ttatcgacta cctgcgcacc gggcaggaga tgccgatgga aatggcggat 2521 cttaatgcag tactcggtga ggtgattgct gccgaaagtg gctatgagcg ggaaattgaa 2581 accgcgcttt accccggcag cattgaagtg aaaatgcacc cgctgtcgat caaacgcgcg 2641 gtggcgaata tggtggtcaa cgccgcccgt tatggcaatg gctggatcaa agtcagcagc 2701 ggaacggagc cgaatcgcgc ctggttccag gtggaagatg acggtccggg aattgcgccg 2761 gaacaacgta agcacctgtt ccagccgttt gtccgcggcg acagtgcgcg caccattagc 2821 ggcacgggat tagggctggc aattgtgcag cgtatcgtgg ataaccataa cgggatgctg 2881 gagcttggca ccagcgagcg gggcgggctt tccattcgcg cctggctgcc agtgccggta 2941 acgcgggcgc agggcacgac aaaagaaggg taatactaga gccaggcatc aaataaaacg 3001 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 3061 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata //