##gff-version 3
##sequence-region BBa_J58122 1 3110
BBa_J58122	.	terminator	1214	1254	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J58122	.	stem_loop	1221	1240	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J58122	.	engineered_region	1214	1254	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J58122	.	stop_codon	1247	1247	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J58122	.	polyA_site	1241	1254	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J58122	.	terminator	1126	1205	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J58122	.	engineered_region	1126	1205	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J58122	.	stem_loop	1137	1180	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J58122	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_J58122	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J58122	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J58122	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J58122	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J58122	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J58122	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J58122	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J58122	.	ribosome_entry_site	1471	1482	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J58122	.	conserved_region	1475	1478	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J58122	.	CDS	227	1117	.	+	0	ID=BBa_J58105;Name=BBa_J58105
BBa_J58122	.	terminator	2982	3061	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J58122	.	engineered_region	2982	3061	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J58122	.	stem_loop	2993	3036	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J58122	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J58122	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J58122	.	promoter	1263	1462	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_J58122	.	engineered_region	1263	1462	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J58122	.	non_covalent_binding_site	1428	1462	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J58122	.	CDS	1263	1350	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J58122	.	start_codon	1435	1435	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J58122	.	non_covalent_binding_site	1351	1388	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J58122	.	promoter	1399	1404	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J58122	.	promoter	1423	1428	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J58122	.	terminator	3070	3110	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J58122	.	stem_loop	3077	3096	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J58122	.	engineered_region	3070	3110	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J58122	.	stop_codon	3103	3103	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J58122	.	polyA_site	3097	3110	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J58122	.	CDS	1489	2973	.	+	0	ID=BBa_J58104;Name=BBa_J58104
>BBa_J58122
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaacatgaa
aaaactggctaccctggtttccgctgttgcgctaagcgccaccgtcagtgcgaatgcgatggcaaaagacaccatcgcg
ctggtggtcgaaacgcttaacaaaccggacaacgtatcgctgaaagatggcgcgcagaaagaggcggataaacttggct
ataacctggtggtgctggactcccagaacaacccggcgaaagagctggcgaacgtgcaggacttaaccgttcgcggcac
aaaaattctgctgattgttccgaccgactccgacgcagtgggtaatgctgtgaagatggctaaccaggcgaacatcccg
gttatcactcttaaacgccaggcaacgaaaggtgaagtggtgagccacattgctgcggataacgtactgggcggcaaaa
tcgctggtgattacatcgcgaagaaagcgggtgaaggtgccaaagttatcgagctgcaaggcaaagctggtacatccgc
agcccgtgaactgggcgaaggcttccagcaggccgttgctgctcacaagtttaatgttcttgccagccagccagcagat
gaagatcgcattaaaggtttgaacgtaatgcagaacctgttgaccgctcatccggatgttcaggctgtattcgcgcagc
aggatgaaatggcgctgggcgcgctgcgcgcactgcaaactgccggtaaatcggatgtgatggtcgtcggagacgttgg
tacaccggatggcgaaaaagcggtgaatgatggcaaactagcagcgactatcgctgaactacccgatcagattggcgcg
aaaggcgtcgaaaccgcagataaagtgctgaaaggcgagaaagttcaggctaagtatccggttgatctgaaactggttg
ttaagcagtagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagca
atacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgg
gcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcg
tatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaatacaactc
cctcacagcgattaggttttttgcatcacatcaggttggttccgttatttgcctgcattctaggcggtatcttagttct
attcgcattaagttcagccctggctggctatttcctctggcaggccgatcgcgatcagcgtgatgttactgcggagatt
gagattcggaccgggttagcgaacagttcagattttttgcgttcagcccggatcaatatgattcaggccggggctgcga
gtcgtattgcggaaatggaagcaatgaagcgaaatattgcgcaagccgaatcggagattaaacagtcgcagcaaggtta
tcgtgcttatcagaatcgaccggtgaaaacacctgctgatgaagccctcgacactgaattaaatcaacgctttcaggct
tatatcacgggtatgcaacctatgttgaaatatgccaaaaatggcatgtttgaagcgattatcaatcatgaaagtgagc
agatccgaccgctggataatgcttataccgatattttgaacaaagccgttaagatacgtagcaccagagccaaccaact
ggcggaactggcccatcagcgcacccgcctgggtgggatgttcatgattggcgcgtttgtgcttgccctggtcatgacg
ctgataacatttatggtgctacgtcggatcgtcattcgtccactgcaacatgccgcacaacggattgaaaaaatcgcca
gtggcgatctgacgatgaatgatgaaccggcgggtcgtaatgaaatcggtcgcttaagtcgtcatttacagcatatggc
ggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacg
cgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagt
gcaacgccatcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgc
agtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaa
gtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctgga
tcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaaca
acgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaatt
gtgcagcgtatcgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcct
ggctgccagtgccggtaacgcgggcgcagggcacgacaaaagaagggtaatactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttata