LOCUS BBa_J58123 7908 bp DNA linear 03-May-2024 DEFINITION Vanillin cellular biosensor ACCESSION BBa_J58123 VERSION BBa_J58123.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 CDS 227..1117 /label=BBa_J58105 stem_loop 1137..1180 /label=stem_loop misc_feature 1126..1205 /label=BBa_B0010 terminator 1126..1205 /label=BBa_B0010 stem_loop 1221..1240 /label=T7 TE misc_feature 1247..1247 /label=stop polyA_site 1241..1254 /label=polya misc_feature 1214..1254 /label=BBa_B0012 terminator 1214..1254 /label=BBa_B0012 CDS 1263..1350 /label=end of LacI coding region (inactive) misc_feature 1263..1462 /label=BBa_R0010 misc_feature 1351..1388 /label=CAP binding site promoter 1399..1404 /label=-35 promoter 1423..1428 /label=-10 misc_feature 1428..1462 /label=LacI binding site misc_feature 1435..1435 /label=start promoter 1263..1462 /label=BBa_R0010 misc_feature 1475..1478 /label=conserved RBS 1471..1482 /label=BBa_B0034 CDS 1489..2973 /label=BBa_J58104 stem_loop 2993..3036 /label=stem_loop misc_feature 2982..3061 /label=BBa_B0010 terminator 2982..3061 /label=BBa_B0010 stem_loop 3077..3096 /label=T7 TE misc_feature 3103..3103 /label=stop polyA_site 3097..3110 /label=polya misc_feature 3070..3110 /label=BBa_B0012 terminator 3070..3110 /label=BBa_B0012 misc_feature 1..3110 /label=BBa_J58122 misc_feature 3131..3148 /label=C1 OmpR misc_feature 3152..3169 /label=C2 OmpR misc_feature 3172..3189 /label=C3 OmpR promoter 3193..3198 /label=-35 promoter 3216..3221 /label=-10 promoter 3119..3226 /label=BBa_R0082 misc_feature 3239..3242 /label=conserved RBS 3235..3246 /label=BBa_B0034 CDS 3253..3885 /label=BBa_J58112 stem_loop 3905..3948 /label=stem_loop misc_feature 3894..3973 /label=BBa_B0010 terminator 3894..3973 /label=BBa_B0010 stem_loop 3989..4008 /label=T7 TE misc_feature 4015..4015 /label=stop polyA_site 4009..4022 /label=polya misc_feature 3982..4022 /label=BBa_B0012 terminator 3982..4022 /label=BBa_B0012 misc_feature 3119..4022 /label=BBa_J58116 misc_feature 4043..4060 /label=C1 OmpR misc_feature 4067..4074 /label=linker promoter 4075..4080 /label=-35 promoter 4098..4103 /label=-10 misc_feature 4071..4071 /label=A promoter 4031..4108 /label=BBa_R0083 misc_feature 4121..4124 /label=conserved RBS 4117..4128 /label=BBa_B0034 CDS 4138..4754 /label=tetR misc_feature 4755..4788 /label=SsrA misc_feature 4795..4819 /label=Help:Barcodes CDS 4135..4794 /label=BBa_C0040 stem_loop 4839..4882 /label=stem_loop misc_feature 4828..4907 /label=BBa_B0010 terminator 4828..4907 /label=BBa_B0010 stem_loop 4923..4942 /label=T7 TE misc_feature 4949..4949 /label=stop polyA_site 4943..4956 /label=polya misc_feature 4916..4956 /label=BBa_B0012 terminator 4916..4956 /label=BBa_B0012 misc_feature 4965..4983 /label=TetR 1 misc_feature 4965..5018 /label=BBa_R0040 promoter 4984..4989 /label=-35 misc_feature 4990..5008 /label=TetR 2 promoter 5007..5012 /label=-10 promoter 4965..5018 /label=BBa_R0040 misc_feature 4117..5018 /label=BBa_Q04400 misc_feature 4031..5018 /label=BBa_J58117 misc_feature 5031..5034 /label=conserved RBS 5027..5038 /label=BBa_B0034 CDS 5048..5755 /label=cI lambda misc_feature 5756..5788 /label=LVA misc_feature 5795..5819 /label=Help:Barcodes CDS 5045..5794 /label=BBa_C0051 stem_loop 5839..5882 /label=stem_loop misc_feature 5828..5907 /label=BBa_B0010 terminator 5828..5907 /label=BBa_B0010 stem_loop 5923..5942 /label=T7 TE misc_feature 5949..5949 /label=stop polyA_site 5943..5956 /label=polya misc_feature 5916..5956 /label=BBa_B0012 terminator 5916..5956 /label=BBa_B0012 misc_feature 5027..5956 /label=BBa_P0451 misc_feature 4031..5956 /label=BBa_J58119 misc_feature 5967..5988 /label=CRP binding site misc_feature 6021..6038 /label=lam-cI OR2 binding site misc_feature 5989..6013 /label=spacer from I12007 (1-11, 59-72) promoter 6036..6041 /label=-35 promoter 6059..6064 /label=-10 misc_feature 6014..6020 /label=spacer from I12007 (26-32) misc_feature 6042..6058 /label=spacer from I12007 (54-70) misc_feature 6065..6070 /label=spacer from I12007 (77-82) promoter 5965..6070 /label=BBa_J58100 misc_feature 6083..6086 /label=conserved RBS 6079..6090 /label=BBa_B0034 CDS 6097..6771 /label=mrfp1 misc_feature 6778..6802 /label=Help:Barcodes CDS 6097..6777 /label=BBa_E1010 stem_loop 6822..6865 /label=stem_loop misc_feature 6811..6890 /label=BBa_B0010 terminator 6811..6890 /label=BBa_B0010 stem_loop 6906..6925 /label=T7 TE misc_feature 6932..6932 /label=stop polyA_site 6926..6939 /label=polya misc_feature 6899..6939 /label=BBa_B0012 terminator 6899..6939 /label=BBa_B0012 misc_feature 6079..6939 /label=BBa_I13507 misc_feature 5965..6939 /label=BBa_J58101 misc_feature 3119..6939 /label=BBa_J58120 misc_feature 6960..6977 /label=C1 OmpR misc_feature 6984..6991 /label=linker promoter 6992..6997 /label=-35 promoter 7015..7020 /label=-10 misc_feature 6988..6988 /label=A promoter 6948..7025 /label=BBa_R0083 misc_feature 7038..7041 /label=conserved RBS 7034..7045 /label=BBa_B0034 CDS 7052..7771 /label=GFP protein CDS 7052..7771 /label=BBa_E0040 stem_loop 7791..7834 /label=stem_loop misc_feature 7780..7859 /label=BBa_B0010 terminator 7780..7859 /label=BBa_B0010 stem_loop 7875..7894 /label=T7 TE misc_feature 7901..7901 /label=stop polyA_site 7895..7908 /label=polya misc_feature 7868..7908 /label=BBa_B0012 terminator 7868..7908 /label=BBa_B0012 misc_feature 6948..7908 /label=BBa_J58103 misc_feature 3119..7908 /label=BBa_J58121 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga acatgaaaaa 241 actggctacc ctggtttccg ctgttgcgct aagcgccacc gtcagtgcga atgcgatggc 301 aaaagacacc atcgcgctgg tggtcgaaac gcttaacaaa ccggacaacg tatcgctgaa 361 agatggcgcg cagaaagagg cggataaact tggctataac ctggtggtgc tggactccca 421 gaacaacccg gcgaaagagc tggcgaacgt gcaggactta accgttcgcg gcacaaaaat 481 tctgctgatt gttccgaccg actccgacgc agtgggtaat gctgtgaaga tggctaacca 541 ggcgaacatc ccggttatca ctcttaaacg ccaggcaacg aaaggtgaag tggtgagcca 601 cattgctgcg gataacgtac tgggcggcaa aatcgctggt gattacatcg cgaagaaagc 661 gggtgaaggt gccaaagtta tcgagctgca aggcaaagct ggtacatccg cagcccgtga 721 actgggcgaa ggcttccagc aggccgttgc tgctcacaag tttaatgttc ttgccagcca 781 gccagcagat gaagatcgca ttaaaggttt gaacgtaatg cagaacctgt tgaccgctca 841 tccggatgtt caggctgtat tcgcgcagca ggatgaaatg gcgctgggcg cgctgcgcgc 901 actgcaaact gccggtaaat cggatgtgat ggtcgtcgga gacgttggta caccggatgg 961 cgaaaaagcg gtgaatgatg gcaaactagc agcgactatc gctgaactac ccgatcagat 1021 tggcgcgaaa ggcgtcgaaa ccgcagataa agtgctgaaa ggcgagaaag ttcaggctaa 1081 gtatccggtt gatctgaaac tggttgttaa gcagtagtac tagagccagg catcaaataa 1141 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg 1201 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag 1261 agcaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga 1321 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac 1381 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt 1441 gagcggataa caatttcaca catactagag aaagaggaga aatactagat gaatacaact 1501 ccctcacagc gattaggttt tttgcatcac atcaggttgg ttccgttatt tgcctgcatt 1561 ctaggcggta tcttagttct attcgcatta agttcagccc tggctggcta tttcctctgg 1621 caggccgatc gcgatcagcg tgatgttact gcggagattg agattcggac cgggttagcg 1681 aacagttcag attttttgcg ttcagcccgg atcaatatga ttcaggccgg ggctgcgagt 1741 cgtattgcgg aaatggaagc aatgaagcga aatattgcgc aagccgaatc ggagattaaa 1801 cagtcgcagc aaggttatcg tgcttatcag aatcgaccgg tgaaaacacc tgctgatgaa 1861 gccctcgaca ctgaattaaa tcaacgcttt caggcttata tcacgggtat gcaacctatg 1921 ttgaaatatg ccaaaaatgg catgtttgaa gcgattatca atcatgaaag tgagcagatc 1981 cgaccgctgg ataatgctta taccgatatt ttgaacaaag ccgttaagat acgtagcacc 2041 agagccaacc aactggcgga actggcccat cagcgcaccc gcctgggtgg gatgttcatg 2101 attggcgcgt ttgtgcttgc cctggtcatg acgctgataa catttatggt gctacgtcgg 2161 atcgtcattc gtccactgca acatgccgca caacggattg aaaaaatcgc cagtggcgat 2221 ctgacgatga atgatgaacc ggcgggtcgt aatgaaatcg gtcgcttaag tcgtcattta 2281 cagcatatgg cggctggtgt taagcaactg gcggatgacc gcacgctgct gatggcgggg 2341 gtaagtcacg acttgcgcac gccgctgacg cgtattcgcc tggcgactga gatgatgagc 2401 gagcaggatg gctatctggc agaatcgatc aataaagata tcgaagagtg caacgccatc 2461 attgagcagt ttatcgacta cctgcgcacc gggcaggaga tgccgatgga aatggcggat 2521 cttaatgcag tactcggtga ggtgattgct gccgaaagtg gctatgagcg ggaaattgaa 2581 accgcgcttt accccggcag cattgaagtg aaaatgcacc cgctgtcgat caaacgcgcg 2641 gtggcgaata tggtggtcaa cgccgcccgt tatggcaatg gctggatcaa agtcagcagc 2701 ggaacggagc cgaatcgcgc ctggttccag gtggaagatg acggtccggg aattgcgccg 2761 gaacaacgta agcacctgtt ccagccgttt gtccgcggcg acagtgcgcg caccattagc 2821 ggcacgggat tagggctggc aattgtgcag cgtatcgtgg ataaccataa cgggatgctg 2881 gagcttggca ccagcgagcg gggcgggctt tccattcgcg cctggctgcc agtgccggta 2941 acgcgggcgc agggcacgac aaaagaaggg taatactaga gccaggcatc aaataaaacg 3001 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 3061 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagtc 3121 ccttgcattt acattttgaa acatctatag cgataaatga aacatcttaa aagttttagt 3181 atcatattcg tgttggatta ttctgcattt ttggggagaa tggacttact agagaaagag 3241 gagaaatact agatggtgct tggcaaaccg caaacagacc cgactctcga atggttcttg 3301 tctcattgcc acattcataa gtacccatcc aagagcacgc ttattcacca gggtgaaaaa 3361 gcggaaacgc tgtactacat cgttaaaggc tctgtggcag tgctgatcaa agacgaagag 3421 ggtaaagaaa tgatcctctc ctatctgaat cagggtgatt ttattggcga actgggcctg 3481 tttgaagagg gccaggaacg tagcgcatgg gtacgtgcga aaaccgcctg tgaagtggct 3541 gaaatttcgt acaaaaaatt tcgccaattg attcaggtaa acccggacat tctgatgcgt 3601 ttgtctgcac agatggcgcg tcgtctgcaa gtcacttcag agaaagtggg caacctggcg 3661 ttcctcgacg tgacgggccg cattgcacag actctgctga atctggcaaa acaaccagac 3721 gctatgactc acccggacgg tatgcaaatc aaaattaccc gtcaggaaat tggtcagatt 3781 gtcggctgtt ctcgtgaaac cgtgggacgc attctgaaga tgctggaaga tcagaacctg 3841 atctccgcac acggtaaaac catcgtcgtt tacggcactc gttaatacta gagccaggca 3901 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 3961 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 4021 tatactagag tcccttgcat ttacattttg aaacatctat agcgatctct tgagttggat 4081 tattctgcat ttttggggag aatggactta ctagagaaag aggagaaata ctagatgtcc 4141 agattagata aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc 4201 gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat 4261 tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg 4321 caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac 4381 gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt 4441 acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa 4501 caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta 4561 ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact 4621 actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca 4681 gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa 4741 tgtgaaagtg ggtccgctgc aaacgacgaa aactacgctt tagtagctta ataacactga 4801 tagtgctagt gtagatcact actagagcca ggcatcaaat aaaacgaaag gctcagtcga 4861 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 4921 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta tcagtgatag 4981 agattgacat ccctatcagt gatagagata ctgagcacta ctagagaaag aggagaaata 5041 ctagatgagc acaaaaaaga aaccattaac acaagagcag cttgaggacg cacgtcgcct 5101 taaagcaatt tatgaaaaaa agaaaaatga acttggctta tcccaggaat ctgtcgcaga 5161 caagatgggg atggggcagt caggcgttgg tgctttattt aatggcatca atgcattaaa 5221 tgcttataac gccgcattgc ttgcaaaaat tctcaaagtt agcgttgaag aatttagccc 5281 ttcaatcgcc agagaaatct acgagatgta tgaagcggtt agtatgcagc cgtcacttag 5341 aagtgagtat gagtaccctg ttttttctca tgttcaggca gggatgttct cacctgagct 5401 tagaaccttt accaaaggtg atgcggagag atgggtaagc acaaccaaaa aagccagtga 5461 ttctgcattc tggcttgagg ttgaaggtaa ttccatgacc gcaccaacag gctccaagcc 5521 aagctttcct gacggaatgt taattctcgt tgaccctgag caggctgttg agccaggtga 5581 tttctgcata gccagacttg ggggtgatga gtttaccttc aagaaactga tcagggatag 5641 cggtcaggtg tttttacaac cactaaaccc acagtaccca atgatcccat gcaatgagag 5701 ttgttccgtt gtggggaaag ttatcgctag tcagtggcct gaagagacgt ttggcgctgc 5761 aaacgacgaa aactacgctt tagtagctta ataacgctga tagtgctagt gtagatcgct 5821 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 5881 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg 5941 cctttctgcg tttatatact agagagaaat gtgatctaga tcacatttgc aaccattata 6001 aatagtggtg ataaaatagt caacacgcac ggtgttagat atttataaat agtggtgata 6061 gatttaacgt tactagagaa agaggagaaa tactagatgg cttcctccga agacgttatc 6121 aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa 6181 atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt 6241 accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt 6301 tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg 6361 gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc 6421 caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac 6481 ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa 6541 cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa 6601 gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag 6661 ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac 6721 accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc 6781 tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga aaggctcagt 6841 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 6901 acactggctc accttcgggt gggcctttct gcgtttatat actagagtcc cttgcattta 6961 cattttgaaa catctatagc gatctcttga gttggattat tctgcatttt tggggagaat 7021 ggacttacta gagaaagagg agaaatacta gatgcgtaaa ggagaagaac ttttcactgg 7081 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag 7141 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac 7201 tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg 7261 ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga 7321 aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc 7381 tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt 7441 taaagaagat ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt 7501 atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa 7561 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga 7621 tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga 7681 tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac 7741 acatggcatg gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa 7801 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 7861 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata //