BBa_B0033
1
BBa_B0033
RBS.4 (weaker) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weaker RBS based on Ron Weiss thesis. Strengths relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-3" in figure 4-14 of thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7028
1
BBa_B0033
range7028
1
1
11
annotation1714
1
RBS
range1714
1
7
10
annotation1713
1
RBS-4\Weaker
range1713
1
1
11
BBa_I12007
1
Prm +
Modified lambda Prm promoter (OR-3 obliterated)
2004-07-14T11:00:00Z
2015-08-31T04:07:31Z
Shih and Gussin (1983)
Released HQ 2013
Lambda Prm promoter modified to be activated but not repressed by the lambda repressor (cI)
false
true
_3_
0
103
7
In stock
false
In wild type lambda phage, the OR3 site (-27 to -11) reads "tatcccttgcggtgata" on the sense strand of DNA. To prevent lambda repressor (cI) from binding to this site, the 4th through 10th nt of OR3 were replaced by the 7 nt between OR2 and OR1, "aaatagt" (-57 to -51), which in effect mutates 5 nt of the promoter. These nt were chosen to be about halfway between the -10 and -35 boxes of the promoter. Furthermore, since these nt already act as a spacer in wild-type phage, it is hoped they will not create undesired interactions.
true
Hans
annotation937701
1
OR1
range937701
1
9
25
annotation937704
1
mutate to obliterate OR3
range937704
1
59
65
annotation937703
1
-35
range937703
1
48
53
annotation937702
1
OR2
range937702
1
33
49
annotation937705
1
-10
range937705
1
71
76
BBa_J59001
1
BBa_J59001
tuba
2006-08-28T11:00:00Z
2015-08-31T02:02:59Z
none
none
false
false
_93_
0
1162
93
Not in stock
false
none
false
Fatos tuba
annotation1898348
1
tuba
range1898348
1
1
25
BBa_J59003
1
BBa_J59003
tuseh DNA
2006-08-28T11:00:00Z
2015-08-31T02:02:59Z
none
none
false
false
_93_
0
1163
93
Not in stock
false
none
false
sehnaz ozatay
component1898357
1
BBa_B0033
component1898366
1
BBa_I12007
component1898360
1
BBa_J59001
annotation1898366
1
BBa_I12007
range1898366
1
62
143
annotation1898360
1
BBa_J59001
range1898360
1
20
53
annotation1898357
1
BBa_B0033
range1898357
1
1
11
BBa_J59001_sequence
1
attttcgtttctgaaaaattttcgtttctgaaaa
BBa_I12007_sequence
1
gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgt
BBa_B0033_sequence
1
tcacacaggac
BBa_J59003_sequence
1
tcacacaggactactagagattttcgtttctgaaaaattttcgtttctgaaaatactagaggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z