BBa_J61001 1 R6K [R6K] Origin of replication 2006-07-30T11:00:00Z 2015-08-31T02:02:59Z <tt> PCR ca1011F/R on pG80ko (445 bp, EcoRI/SpeI)<br> Sub into pSB1A2-I13522 (EcoRI/SpeI)<br> Product is Bca1011<br> ---- ca1011F Forward Biobricking of R6K<br> GGACTgaattcgcggccgcttctagagtgattcgcacgggcccatg<br> ca1011R Reverse biobricking of R6K<br> ccgctactagtaGCAGTTCAACCTGTTGATAG<br> </tt> Released HQ 2013 R6K origin of replication requires a pir+ or pir116 strain for replication in the absence of a second origin. In most E. coli strains, this is a silent feature. {pSB1A2-Bca1011} false false _95_ 0 483 95 In stock false N/A true John Anderson annotation1892271 1 R6K range1892271 1 27 406 BBa_J61001_sequence 1 tgattcgcacgggcccatggctaattcccatgtcagccgttaagtgttcctgtgtcactcaaaattgctttgagaggctctaagggcttctcagtgcgttacatccctggcttgttgtccacaaccgttaaaccttaaaagctttaaaagccttatatattcttttttttcttataaaacttaaaaccttagaggctatttaagttgctgatttatattaattttattgttcaaacatgagagcttagtacgtgaaacatgagagcttagtacgttagccatgagagcttagtacgttagccatgagggtttagttcgttaaacatgagagcttagtacgttaaacatgagagcttagtacgtgaaacatgagagcttagtacgtactatcaacaggttgaactgc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z