##gff-version 3
##sequence-region BBa_J61002 2 2948
BBa_J61002	.	sequence_feature	2	7	.	+	0	ID=annotation1975716;Name=SpeI
BBa_J61002	.	engineered_region	741	869	.	+	0	ID=annotation1892274;Name=BBa_B0015
BBa_J61002	.	ribosome_entry_site	9	20	.	+	0	ID=annotation1975766;Name=RBS
BBa_J61002	.	promoter	2728	2756	.	+	0	ID=annotation1892272;Name=beta-lactamase (ampR, bla) promoter
BBa_J61002	.	CDS	27	707	.	+	0	ID=annotation1892273;Name=mRFP
BBa_J61002	.	sequence_feature	1052	1671	.	+	0	ID=annotation1975714;Name=pBR322 origin
BBa_J61002	.	sequence_feature	878	885	.	+	0	ID=annotation1975718;Name=NotI
BBa_J61002	.	primer_binding_site	2809	2828	.	+	0	ID=annotation1975769;Name=Verification forward (VF2) primer binding site
BBa_J61002	.	sequence_alteration	2941	2941	.	+	0	ID=annotation1975921;Name=Ambiguity; should be a T
BBa_J61002	.	sequence_feature	2927	2948	.	+	0	ID=annotation1975715;Name=BioBrick prefix
BBa_J61002	.	engineered_region	9	20	.	+	0	ID=annotation1975767;Name=BBa_B0034 RBS
BBa_J61002	.	sequence_feature	885	890	.	+	0	ID=annotation1975717;Name=PstI
BBa_J61002	.	primer_binding_site	948	967	.	+	0	ID=annotation1975768;Name=Verification reverse (VR) primer binding site
BBa_J61002	.	stem_loop	752	795	.	+	0	ID=annotation1975713;Name=rrnB terminator
BBa_J61002	.	sequence_feature	1049	1731	.	+	0	ID=annotation1892275;Name=ColE1 origin
BBa_J61002	.	CDS	1826	2686	.	+	0	ID=annotation1892276;Name=beta-lactamase ampicillin resistance marker
>BBa_J61002
tactagtgaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtat
ggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgct
aaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaag
cttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttat
gaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaa
ctgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgta
tgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctga
agttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacc
tcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgct
gatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgt
tttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
tactagaagcggccgctgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcag
ctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagca
aaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaat
cgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgc
gctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatag
ctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagccc
gaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagcca
ctggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacac
tagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggc
aaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaag
atcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatc
aaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttgg
tctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgac
tccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacg
ctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatcc
gcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttg
ccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcg
agttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggcc
gcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtga
ctggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacggga
taataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatc
ttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcg
tttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcat
actcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttag
aaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatga
cattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgattt
ctggaattcgcggccgcatctagag