BBa_J61003 1 BBa_J61003 EN-Ptet-rbs-X-[GFP]-SNP "ORF Expresser" 2006-07-30T11:00:00Z 2015-08-31T02:02:59Z <tt> PCR KB001/ca1021R on pSB1A2-J01022 (250 bp, EcoRI/XbaI)<br> sub into pSB1A2-E0040(GFP;DNA-1-5H) (EcoRI/XbaI)<br> Product is pBca1021-E0040<br> ---- ca1021R Reverse b0034 XbaI<br> AGCCATCTAGAATTTCTCCTCTTTCTCTAG<br> KB001 Forward EcoRI to construct Ptet part-making biobrick plasmid<br> ttctggaattcgcggccgcaactagagccaggcatc<br> </tt> Plasmid for assembly of open reading frame basic parts. A Tet promoter (r0040) and strong ribosome binding site (b0034) lie between the EcoRI and XbaI sites. Insertion of an open reading frame basic part into the XbaI and PstI sites results in constitutive expression but retains basic part utilityin standard assembly. false true _95_ 0 483 95 Not in stock false N/A true John Anderson annotation1975754 1 Verification reverse (VR) primer binding site range1975754 1 79 98 annotation1975749 1 BioBrick suffix range1975749 1 1 21 annotation1975748 1 pBR322 range1975748 1 183 802 annotation1975755 1 BBa_B0034 RBS range1975755 1 2279 2290 annotation1975750 1 EcoRI range1975750 1 2058 2063 annotation1975756 1 RBS range1975756 1 2279 2290 annotation1975753 1 Verification forward (VF2) primer binding site range1975753 1 1940 1959 annotation1892279 1 beta-lactamase (ampR) promoter range1892279 1 1859 1887 annotation1975752 1 XbaI range1975752 1 2292 2297 annotation1975751 1 NotI range1975751 1 2064 2071 annotation1892280 1 ColE1 origin range1892280 1 180 862 annotation1975747 1 rrnB T1 terminator range1975747 1 2091 2134 annotation1892277 1 BBa_B0015 (terminator) range1892277 1 2080 2208 annotation1975757 1 Ptet promoter range1975757 1 2217 2270 annotation1892278 1 BBa_R0040 (Ptet) range1892278 1 2217 2270 annotation1892281 1 beta-lactamase (ampicillin resistance marker) range1892281 1 957 1817 BBa_J61003_sequence 1 tactagtagcggccgctgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcaactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaattctagag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z