BBa_J61017 1 BBa_J61017 Biobrick converter for NotI-part-EcoRI 2006-08-10T11:00:00Z 2015-08-31T02:02:59Z <tt> PCR ca1007F/R on pSB1A2-J01064 (972 bp, EcoRI/SpeI)<br> PCR ca1008F/ca1009R on pSB1A2-J01064 (972 bp, EcoRI/SpeI)<br> PCR ca1007F/ca1009R on pSB1A2-J01064 (972 bp, EcoRI/SpeI)<br> Sub into pSB1A2 (EcoRI/SpeI)<br> Products are:<br> Bca1007 NotI/BamHI transfer<br> Bca1008 BamHI/EcoRI transfer<br> Bca1009 NotI/EcoRI transfer<br> ------------------------------------------------ ca1007F Forward EcoRI for 5' PspOMI NotI converter<br> ctctggaattcgcggccgcttctagagggccctccctatcagtgatagagattg<br> ca1007R Reverse SpeI for 3' BglII BamHI converter<br> ccgctactagtagatcttataaacgcagaaaggcccac<br> ca1008F Forward EcoRI for 5' BglII BamHI converter<br> ctctggaattcgcggccgcttctagaagatcttccctatcagtgatagagattg<br> ca1009R Reverse SpeI for 3' MfeI EcoRI converter<br> ccgctactagtcaattgtataaacgcagaaaggcccac<br> Inserting a NotI/EcoRI fragment into the PspOMI/MfeI sites of J61017 destroys the restriction sites and introduces standard Biobrick flanking sequence. In the process, the OnRFP cassette is destroyed allowing Red to White screening of colonies. The registry sequence is blunt relative to XbaI/SpeI. [[Media:pSB1A2-Bca1009.ogg|ApE file:{pSB1A2-Bca1009}]] false false _95_ 0 483 95 In stock false N/A false John Anderson BBa_J61017_sequence 1 gggccctccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatacaattg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z