BBa_J61023
1
TnP
Tn5 Transposase [Tnp1-PN] (with PstI and NotI)
2006-09-20T11:00:00Z
2015-08-31T02:02:59Z
PCR ca1014F/R on pRL27 (1763 bp, EcoRI/SpeI)<br>
Sub into pSB1A2-I13521 (EcoRI/SpeI)<br>
Product is Bca1014 *internal PstI and NotI sites<br>
Tn5 transposase-expressing cassette from pRL. Cassette includes a complete promoter/rbs/orf cassette. The promoter is Ptet, but not derived from r0040. There are PstI and NotI sites in this part, so care must be taken when doing assembly not to use those sites.
pSB1A2-Bca1014
false
false
_95_
0
483
95
It's complicated
false
There are PstI and NotI sites in this part, so care must be taken when doing assembly not to use those sites.
true
John Anderson
BBa_J61023_sequence
1
tgattctccgccagcatggcttcggccagtgcgtcgagcagcgcccgcttgttcctgaagtgccagtaaagcgccggctgctgaacccccaaccgttccgccagtttgcgtgtcgtcagaccgtctacgccgacctcgttcaacaggtctagggcggcacggatcactgtattcggctgcaactttgtcatgcttgacactttatcactgataaacataatatgtccaccaacttatcagtgataaagaatccgcgcgttcaatcggaccagcggaggctggtccggaggccacatatgataacttctgctcttcatcgtgcggccgactgggctaaatctgtgttctcttcggcggcgctgggtgatcctcgccgtactgcccgcttggttaacgtcgccgcccaattggcaaaatattctggtaaatcaataaccatctcatcagagggtagtaaagccgcccaggaaggcgcttaccgatttatccgcaatcccaacgtttctgccgaggcgatcagaaaggctggcgccatgcaaacagtcaagttggctcaggagtttcccgaactgctggccattgaggacaccacctctttgagttatcgccaccaggtcgccgaagagcttggcaagctgggctctattcaggataaatcccgcggatggtgggttcactccgttctcttgctcgaggccaccacattccgcaccgtaggattactgcatcaggagtggtggatgcgcccggatgaccctgccgatgcggatgaaaaggagagtggcaaatggctggcagcggccgcaactagccggttacgcatgggcagcatgatgagcaacgtgattgcggtctgtgaccgcgaagccgatattcatgcttatctgcaggacaaactggcgcataacgagcgcttcgtggtgcgctccaagcacccacgcaaggacgtagagtctgggttgtatctgtacgaccatctgaagaaccaaccggagttgggtggctatcagatcagcattccgcaaaagggcgtggtggataaacgcggtaaacgtaaaaatcgaccagcccgcaaggcgagcttgagcctgcgcagtgggcgcatcacgctaaaacaggggaatatcacgctcaacgcggtgctggccgaggagattaacccgcccaagggtgagaccccgttgaaatggttgttgctgaccagcgaaccggtcgagtcgctagcccaagccttgcgcgtcatcgacatttatacccatcgctggcggatcgaggagttccataaggcatggaaaaccggagcaggagccgagaggcaacgcatggaggagccggataatctggagcggatggtctcgatcctctcgtttgttgcggtcaggctgttacagctcagagaaagcttcacgccgccgcaagcactcagggcgcaagggctgctaaaggaagcggaacacgtagaaagccagtccgcagaaacggtgctgaccccggatgaatgtcagctactgggctatctggacaagggaaaacgcaagcgcaaagagaaagcaggtagcttgcagtgggcttacatggcgatagctagactgggcggttttatggacagcaagcgaaccggaattgccagctggggcgccctctgggaaggttgggaagccctgcaaagtaaactggatggctttcttgccgccaaggatctgatggcgcaggggatcaagatctgatccgtc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z