##gff-version 3 ##sequence-region BBa_J61034 1 1417 BBa_J61034 . engineered_region 47 1417 . + 0 ID=BBa_Q04121;Name=BBa_Q04121 BBa_J61034 . terminator 1226 1354 . + 0 ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q04121 BBa_J61034 . terminator 1314 1354 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015 BBa_J61034 . engineered_region 1314 1354 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_J61034 . stop_codon 1347 1347 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_J61034 . stem_loop 1321 1340 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_J61034 . polyA_site 1341 1354 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_J61034 . terminator 1226 1305 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015 BBa_J61034 . engineered_region 1226 1305 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_J61034 . stem_loop 1237 1280 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_J61034 . promoter 1363 1416 . + 0 ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_Q04121 BBa_J61034 . promoter 1405 1410 . + 0 ID=annotation2002;Name=-10;Parent=BBa_R0011 BBa_J61034 . operator 1388 1404 . + 0 ID=annotation2001;Name=lac O1;Parent=BBa_R0011 BBa_J61034 . promoter 1382 1387 . + 0 ID=annotation2000;Name=-35;Parent=BBa_R0011 BBa_J61034 . engineered_region 1363 1416 . + 0 ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011 BBa_J61034 . operator 1365 1381 . + 0 ID=annotation1999;Name=lac O1;Parent=BBa_R0011 BBa_J61034 . CDS 65 1192 . + 0 ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_Q04121 BBa_J61034 . engineered_tag 1193 1217 . + 0 ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012 BBa_J61034 . engineered_region 65 1192 . + 0 ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012 BBa_J61034 . CDS 65 1192 . + 0 ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012 BBa_J61034 . tag 1154 1192 . + 0 ID=annotation1722;Name=LVA;Parent=BBa_C0012 BBa_J61034 . ribosome_entry_site 47 58 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04121 BBa_J61034 . conserved_region 51 54 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_J61034 . promoter 1 38 . + 0 ID=BBa_I14033;Name=BBa_I14033 BBa_J61034 . operator 1 38 . + 0 ID=annotation1026174;Name=P(Cat);Parent=BBa_I14033 BBa_J61034 . promoter 27 32 . + 0 ID=annotation1026173;Name=-10;Parent=BBa_I14033 BBa_J61034 . promoter 4 9 . + 0 ID=annotation1026172;Name=-35;Parent=BBa_I14033 >BBa_J61034 ggcacgtaagaggttccaactttcaccataatgaaacatactagagaaagaggagaaatactagatggtgaatgtgaaa ccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagcc acgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaaca actggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcg gcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgta aagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccat tgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattatt ttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgg gcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgat agcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttccc actgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtg cggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacagga ttttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctg ttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgatt cattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagc ttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagact gggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggccttt ctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca