BBa_J61201
1
rbsGenR
[rbsGenR]
2007-01-30T12:00:00Z
2015-08-31T01:56:25Z
(to be filled in)
Gentamicin resistance gene including a medium/weak ribosome binding site, but NO promoter. Intended for use in transposons, to select for elements that fall inside of a transcribed region. The RBS does not appear to be strong enough for this purpose.
false
false
_95_
0
947
95
In stock
true
(to be filled in)
true
Josh Kittleson
annotation1911576
1
GenR
range1911576
1
21
554
BBa_J61202
1
BBa_J61202
[Tn5F][rbsGenR]
2007-01-30T12:00:00Z
2015-08-31T01:56:25Z
See component parts.
Construction intermediate for a transposon selectable for insertion into a transcribed region.
false
false
_95_
0
947
95
It's complicated
false
No special design considerations.
false
Josh Kittleson
component2218368
1
BBa_J61201
component2218366
1
BBa_J61021
annotation2218366
1
BBa_J61021
range2218366
1
1
19
annotation2218368
1
BBa_J61201
range2218368
1
28
587
BBa_J61021
1
Tn5F
[Tn5-for]
2006-09-20T11:00:00Z
2015-08-31T02:02:59Z
Overlap extension of synthetic oligonucleotides
DNA substrate for Tn5 transposase. Flanking a sequence with J61021 on the 5' end and J61022 on the 3' end makes it a functional transposon.
false
false
_95_
0
483
95
It's complicated
false
N/A
false
John Anderson
BBa_J61021_sequence
1
ctgtctcttatacacatct
BBa_J61202_sequence
1
ctgtctcttatacacatcttactagaggatccgttccggaatgtttgatgttatggagcagcaacgatgttacgcagcagggcagtcgccctaaaacaaagttaggtggctcaagtatgggcatcattcgcacatgtaggctcggccctgaccaagtcaaatccatgcgggctgctcttgatcttttcggtcgtgagttcggagacgtagccacctactcccaacatcagccggactccgattacctcgggaacttgctccgtagtaagacattcatcgcgcttgctgccttcgaccaagaagcggttgttggcgctctcgcggcttacgttctgcccaagtttgagcagccgcgtagtgagatctatatctatgatctcgcagtctccggagagcaccggaggcagggcattgccaccgcgctcatcaatctcctcaagcatgaggccaacgcgcttggtgcttatgtgatctacgtgcaagcagattacggtgacgatcccgcagtggctctctatacaaagttgggcatacgggaagaagtgatgcactttgatatcgacccaagtaccgccacctaacgaatt
BBa_J61201_sequence
1
gatccgttccggaatgtttgatgttatggagcagcaacgatgttacgcagcagggcagtcgccctaaaacaaagttaggtggctcaagtatgggcatcattcgcacatgtaggctcggccctgaccaagtcaaatccatgcgggctgctcttgatcttttcggtcgtgagttcggagacgtagccacctactcccaacatcagccggactccgattacctcgggaacttgctccgtagtaagacattcatcgcgcttgctgccttcgaccaagaagcggttgttggcgctctcgcggcttacgttctgcccaagtttgagcagccgcgtagtgagatctatatctatgatctcgcagtctccggagagcaccggaggcagggcattgccaccgcgctcatcaatctcctcaagcatgaggccaacgcgcttggtgcttatgtgatctacgtgcaagcagattacggtgacgatcccgcagtggctctctatacaaagttgggcatacgggaagaagtgatgcactttgatatcgacccaagtaccgccacctaacgaatt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z