LOCUS BBa_J61207 4977 bp DNA linear 22-Nov-2024 DEFINITION TrnspsnPlsmd Tn5F|rbsGenR|SpecR|P_T7|Tn5R||CmR|TnP|OriTr|R6K ACCESSION BBa_J61207 VERSION BBa_J61207.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=BBa_J61021 CDS 48..581 /label=GenR misc_feature 28..587 /label=BBa_J61201 misc_feature 1..587 /label=BBa_J61202 CDS 641..1432 /label=SpR Spectinomycin Resistance misc_feature 596..1483 /label=BBa_J23012 misc_feature 1509..1509 /label=Transcription Start Site misc_feature 1492..1502 /label=Polymerase Binding Region misc_feature 1503..1514 /label=Initiation Region promoter 1492..1514 /label=BBa_R0085 misc_feature 1523..1541 /label=BBa_J61022 misc_feature 596..1541 /label=BBa_J61205 misc_feature 1..1541 /label=BBa_J61206 CDS 1579..2236 /label=CmR misc_feature 1550..2443 /label=BBa_J61000 misc_feature 2452..4184 /label=BBa_J61023 oriT 4193..4563 /label=BBa_J01003 misc_feature 4598..4977 /label=R6K misc_feature 4572..4977 /label=BBa_J61001 misc_feature 1550..4977 /label=BBa_J61204 ORIGIN 1 ctgtctctta tacacatctt actagaggat ccgttccgga atgtttgatg ttatggagca 61 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaggtggc tcaagtatgg 121 gcatcattcg cacatgtagg ctcggccctg accaagtcaa atccatgcgg gctgctcttg 181 atcttttcgg tcgtgagttc ggagacgtag ccacctactc ccaacatcag ccggactccg 241 attacctcgg gaacttgctc cgtagtaaga cattcatcgc gcttgctgcc ttcgaccaag 301 aagcggttgt tggcgctctc gcggcttacg ttctgcccaa gtttgagcag ccgcgtagtg 361 agatctatat ctatgatctc gcagtctccg gagagcaccg gaggcagggc attgccaccg 421 cgctcatcaa tctcctcaag catgaggcca acgcgcttgg tgcttatgtg atctacgtgc 481 aagcagatta cggtgacgat cccgcagtgg ctctctatac aaagttgggc atacgggaag 541 aagtgatgca ctttgatatc gacccaagta ccgccaccta acgaatttac tagagtccaa 601 gcgagctcga tatccgtcgg cttgaacgaa ttgttagaca ttatttgccg actaccttgg 661 tgatctcgcc tttcacgtag tggacaaatt cttccaactg atctgcgcgc gaggccaagc 721 gatcttcttc ttgtccaaga taagcctgtc tagcttcaag tatgacgggc tgatactggg 781 ccggcaggcg ctccattgcc cagtcggcag cgacatcctt cggcgcgatt ttgccggtta 841 ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg ccagcccagt 901 cgggcggcga gttccatagc gttaaggttt catttagcgc ctcaaataga tcctgttcag 961 gaaccggatc aaagagttcc tccgccgctg gacctaccaa ggcaacgcta tgttctcttg 1021 cttttgtcag caagatagcc agatcaatgt cgtacgtggc tggctcgaag atacctgcaa 1081 gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg cttagctgga taacgccacg 1141 gaatgatgtc gtcgtgcaca acaatggtga cttctacagc gcggagaatc tcgctctctc 1201 caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa 1261 gccttacggt caccgtaacc agcaaatcaa tatcactgtg tggcttcagg ccgccatcca 1321 ctgcggagcc gtacaaatgt acggccagca acgtcggttc gagatggcgc tcgatgacgc 1381 caactacctc tgatagttga gtcgatactt cggcgatcac cgcttccctc atgatgttta 1441 actttgtttt agggcgactg ccctgctgcg taacatcgtt gcttactaga gtaatacgac 1501 tcactatagg gagatactag agagatgtgt ataagagaca gtactagagg aataggaact 1561 tcatttaaat ggcgcgcctt acgccccgcc ctgccactca tcgcagtact gttgtattca 1621 ttaagcatct gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg 1681 gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga 1741 agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg 1801 agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac 1861 acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc 1921 agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat 1981 cccatatcac cagctcaccg tctttcattg ccatacgtaa ttccggatga gcattcatca 2041 ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct 2101 ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact 2161 gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag 2221 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgacaac tcaaaaaata 2281 cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg tgccgatcaa 2341 cgtctcattt tcgccaaaag ttggcccagg gcttcccggt atcaacaggg acaccaggat 2401 ttatttattc tgcgaagtga tcttccgtca caggtaggcg cgctactaga gtgattctcc 2461 gccagcatgg cttcggccag tgcgtcgagc agcgcccgct tgttcctgaa gtgccagtaa 2521 agcgccggct gctgaacccc caaccgttcc gccagtttgc gtgtcgtcag accgtctacg 2581 ccgacctcgt tcaacaggtc tagggcggca cggatcactg tattcggctg caactttgtc 2641 atgcttgaca ctttatcact gataaacata atatgtccac caacttatca gtgataaaga 2701 atccgcgcgt tcaatcggac cagcggaggc tggtccggag gccacatatg ataacttctg 2761 ctcttcatcg tgcggccgac tgggctaaat ctgtgttctc ttcggcggcg ctgggtgatc 2821 ctcgccgtac tgcccgcttg gttaacgtcg ccgcccaatt ggcaaaatat tctggtaaat 2881 caataaccat ctcatcagag ggtagtaaag ccgcccagga aggcgcttac cgatttatcc 2941 gcaatcccaa cgtttctgcc gaggcgatca gaaaggctgg cgccatgcaa acagtcaagt 3001 tggctcagga gtttcccgaa ctgctggcca ttgaggacac cacctctttg agttatcgcc 3061 accaggtcgc cgaagagctt ggcaagctgg gctctattca ggataaatcc cgcggatggt 3121 gggttcactc cgttctcttg ctcgaggcca ccacattccg caccgtagga ttactgcatc 3181 aggagtggtg gatgcgcccg gatgaccctg ccgatgcgga tgaaaaggag agtggcaaat 3241 ggctggcagc ggccgcaact agccggttac gcatgggcag catgatgagc aacgtgattg 3301 cggtctgtga ccgcgaagcc gatattcatg cttatctgca ggacaaactg gcgcataacg 3361 agcgcttcgt ggtgcgctcc aagcacccac gcaaggacgt agagtctggg ttgtatctgt 3421 acgaccatct gaagaaccaa ccggagttgg gtggctatca gatcagcatt ccgcaaaagg 3481 gcgtggtgga taaacgcggt aaacgtaaaa atcgaccagc ccgcaaggcg agcttgagcc 3541 tgcgcagtgg gcgcatcacg ctaaaacagg ggaatatcac gctcaacgcg gtgctggccg 3601 aggagattaa cccgcccaag ggtgagaccc cgttgaaatg gttgttgctg accagcgaac 3661 cggtcgagtc gctagcccaa gccttgcgcg tcatcgacat ttatacccat cgctggcgga 3721 tcgaggagtt ccataaggca tggaaaaccg gagcaggagc cgagaggcaa cgcatggagg 3781 agccggataa tctggagcgg atggtctcga tcctctcgtt tgttgcggtc aggctgttac 3841 agctcagaga aagcttcacg ccgccgcaag cactcagggc gcaagggctg ctaaaggaag 3901 cggaacacgt agaaagccag tccgcagaaa cggtgctgac cccggatgaa tgtcagctac 3961 tgggctatct ggacaaggga aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg 4021 cttacatggc gatagctaga ctgggcggtt ttatggacag caagcgaacc ggaattgcca 4081 gctggggcgc cctctgggaa ggttgggaag ccctgcaaag taaactggat ggctttcttg 4141 ccgccaagga tctgatggcg caggggatca agatctgatc cgtctactag aggccgcctt 4201 ttcctcaatc gctcttcgtt cgtctggaag gcagtacacc ttgataggtg ggctgccctt 4261 cctggttggc ttggtttcat cagccatccg cttgccctca tctgttacgc cggcggtagc 4321 cggccagcct cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat aagggacagt 4381 gaagaaggaa cacccgctcg cgggtgggcc tacttcacct atcctgcccg gctgacgccg 4441 ttggatacac caaggaaagt ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa 4501 aaaggatgga tataccgaaa aaatcgctat aatgaccccg aagcagggtt atgcagcgga 4561 aaatactaga gtgattcgca cgggcccatg gctaattccc atgtcagccg ttaagtgttc 4621 ctgtgtcact caaaattgct ttgagaggct ctaagggctt ctcagtgcgt tacatccctg 4681 gcttgttgtc cacaaccgtt aaaccttaaa agctttaaaa gccttatata ttcttttttt 4741 tcttataaaa cttaaaacct tagaggctat ttaagttgct gatttatatt aattttattg 4801 ttcaaacatg agagcttagt acgtgaaaca tgagagctta gtacgttagc catgagagct 4861 tagtacgtta gccatgaggg tttagttcgt taaacatgag agcttagtac gttaaacatg 4921 agagcttagt acgtgaaaca tgagagctta gtacgtacta tcaacaggtt gaactgc //