BBa_J61211
type: ComponentDefinition
persistent identity: BBa J61211
display id: BBa_J61211
version: 1
was generated by: iGEM to SBOL conversion
description: [Tn5F][rbsGenR][FRT][R6K][CmR][FRT][P_T7][Tn5R]
created: 2007-01-31T12:00:00Z
modified: 2015-08-31T01:56:25Z
mutable provenance: see composite parts.
owned by: james
owned by: myers
mutable description: Transposable piece of a transposon bearing suicide plasmid, containing chloramphenicol resistance, an origin of replication for decoding, and a selectable gentimicin marker that only activates when the transposon lands in a transcribed region.
discontinued: false
dominant: false
experience: experience/None
group u list: _95_
m user id: 0
owner id: 947
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: no special considerations.
star: false
creator: Josh Kittleson
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_J61210
component: BBa_J61202
sequence annotation: BBa_J61202
sequence annotation: BBa_J61210
sequence: https://synbiohub.org/public/igem/BBa_J61211_sequence/1

BBa_J61210
type: Component
persistent identity: component2226370
display id: component2226370
version: 1
top level: BBa_J61211
definition: BBa_J61210
access: public

BBa_J61202
type: Component
persistent identity: component2226358
display id: component2226358
version: 1
top level: BBa_J61211
definition: BBa_J61202
access: public

BBa_J61202
type: SequenceAnnotation
persistent identity: annotation2226358
display id: annotation2226358
version: 1
top level: BBa_J61211
location: https://synbiohub.org/public/igem/BBa_J61211/annotation2226358/range2226358/1
component: BBa_J61202

https://synbiohub.org/public/igem/BBa_J61211/annotation2226358/range2226358/1
type: Range
persistent identity: range2226358
display id: range2226358
version: 1
top level: BBa_J61211
direction: direction/reverse
start: 1
end: 587
orientation: inline

BBa_J61210
type: SequenceAnnotation
persistent identity: annotation2226370
display id: annotation2226370
version: 1
top level: BBa_J61211
location: https://synbiohub.org/public/igem/BBa_J61211/annotation2226370/range2226370/1
component: BBa_J61210

https://synbiohub.org/public/igem/BBa_J61211/annotation2226370/range2226370/1
type: Range
persistent identity: range2226370
display id: range2226370
version: 1
top level: BBa_J61211
direction: direction/reverse
start: 596
end: 2045
orientation: inline

BBa_J61202
type: ComponentDefinition
persistent identity: BBa J61202
display id: BBa_J61202
version: 1
was generated by: iGEM to SBOL conversion
description: [Tn5F][rbsGenR]
created: 2007-01-30T12:00:00Z
modified: 2015-08-31T01:56:25Z
mutable provenance: See component parts.
owned by: james
owned by: myers
mutable description: Construction intermediate for a transposon selectable for insertion into a transcribed region.
discontinued: false
dominant: false
experience: experience/None
group u list: _95_
m user id: 0
owner id: 947
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: No special design considerations.
star: false
creator: Josh Kittleson
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J61201
component: BBa_J61021
sequence annotation: BBa_J61021
sequence annotation: BBa_J61201
sequence: https://synbiohub.org/public/igem/BBa_J61202_sequence/1

BBa_J61201
type: Component
persistent identity: component2218368
display id: component2218368
version: 1
top level: BBa_J61202
definition: rbsGenR
access: public

BBa_J61021
type: Component
persistent identity: component2218366
display id: component2218366
version: 1
top level: BBa_J61202
definition: Tn5F
access: public

BBa_J61021
type: SequenceAnnotation
persistent identity: annotation2218366
display id: annotation2218366
version: 1
top level: BBa_J61202
location: https://synbiohub.org/public/igem/BBa_J61202/annotation2218366/range2218366/1
component: BBa_J61021

https://synbiohub.org/public/igem/BBa_J61202/annotation2218366/range2218366/1
type: Range
persistent identity: range2218366
display id: range2218366
version: 1
top level: BBa_J61202
direction: direction/reverse
start: 1
end: 19
orientation: inline

BBa_J61201
type: SequenceAnnotation
persistent identity: annotation2218368
display id: annotation2218368
version: 1
top level: BBa_J61202
location: https://synbiohub.org/public/igem/BBa_J61202/annotation2218368/range2218368/1
component: BBa_J61201

https://synbiohub.org/public/igem/BBa_J61202/annotation2218368/range2218368/1
type: Range
persistent identity: range2218368
display id: range2218368
version: 1
top level: BBa_J61202
direction: direction/reverse
start: 28
end: 587
orientation: inline

BBa_J61210
type: ComponentDefinition
persistent identity: BBa J61210
display id: BBa_J61210
version: 1
was generated by: iGEM to SBOL conversion
description: [FRT][R6K][CmR][FRT][P_T7][Tn5R]
created: 2007-01-31T12:00:00Z
modified: 2015-08-31T01:56:25Z
mutable provenance: see composite parts.
owned by: james
owned by: myers
mutable description: Intermediate for a transposon with a chloramphenicol resistance marker and a replication origin inside of the transposed region.
discontinued: false
dominant: false
experience: experience/None
group u list: _95_
m user id: 0
owner id: 947
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: no special design considerations
star: false
creator: Josh Kittleson
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_J61022
component: BBa_J61020
component: BBa_R0085
component: BBa_J61000
component: BBa_J61001
sequence annotation: BBa_J61020
sequence annotation: BBa_J61001
sequence annotation: BBa_J61000
sequence annotation: BBa_R0085
sequence annotation: BBa_J61022
sequence: https://synbiohub.org/public/igem/BBa_J61210_sequence/1

BBa_J61022
type: Component
persistent identity: component1911676
display id: component1911676
version: 1
top level: BBa_J61210
definition: Tn5R
access: public

BBa_J61020
type: Component
persistent identity: component1911666
display id: component1911666
version: 1
top level: BBa_J61210
definition: FRT
access: public

BBa_R0085
type: Component
persistent identity: component1911675
display id: component1911675
version: 1
top level: BBa_J61210
definition: BBa_R0085
access: public

BBa_J61020
type: Component
persistent identity: component1911671
display id: component1911671
version: 1
top level: BBa_J61210
definition: FRT
access: public

BBa_J61000
type: Component
persistent identity: component1911670
display id: component1911670
version: 1
top level: BBa_J61210
definition: CmR
access: public

BBa_J61001
type: Component
persistent identity: component1911668
display id: component1911668
version: 1
top level: BBa_J61210
definition: R6K
access: public

BBa_J61020
type: SequenceAnnotation
persistent identity: annotation1911666
display id: annotation1911666
version: 1
top level: BBa_J61210
location: https://synbiohub.org/public/igem/BBa_J61210/annotation1911666/range1911666/1
component: BBa_J61020

https://synbiohub.org/public/igem/BBa_J61210/annotation1911666/range1911666/1
type: Range
persistent identity: range1911666
display id: range1911666
version: 1
top level: BBa_J61210
direction: direction/reverse
start: 1
end: 34
orientation: inline

BBa_J61001
type: SequenceAnnotation
persistent identity: annotation1911668
display id: annotation1911668
version: 1
top level: BBa_J61210
location: https://synbiohub.org/public/igem/BBa_J61210/annotation1911668/range1911668/1
component: BBa_J61001

https://synbiohub.org/public/igem/BBa_J61210/annotation1911668/range1911668/1
type: Range
persistent identity: range1911668
display id: range1911668
version: 1
top level: BBa_J61210
direction: direction/reverse
start: 43
end: 448
orientation: inline

BBa_J61000
type: SequenceAnnotation
persistent identity: annotation1911670
display id: annotation1911670
version: 1
top level: BBa_J61210
location: https://synbiohub.org/public/igem/BBa_J61210/annotation1911670/range1911670/1
component: BBa_J61000

https://synbiohub.org/public/igem/BBa_J61210/annotation1911670/range1911670/1
type: Range
persistent identity: range1911670
display id: range1911670
version: 1
top level: BBa_J61210
direction: direction/reverse
start: 457
end: 1350
orientation: inline

BBa_J61020
type: SequenceAnnotation
persistent identity: annotation1911671
display id: annotation1911671
version: 1
top level: BBa_J61210
location: https://synbiohub.org/public/igem/BBa_J61210/annotation1911671/range1911671/1
component: BBa_J61020

https://synbiohub.org/public/igem/BBa_J61210/annotation1911671/range1911671/1
type: Range
persistent identity: range1911671
display id: range1911671
version: 1
top level: BBa_J61210
direction: direction/reverse
start: 1359
end: 1392
orientation: inline

BBa_R0085
type: SequenceAnnotation
persistent identity: annotation1911675
display id: annotation1911675
version: 1
top level: BBa_J61210
location: https://synbiohub.org/public/igem/BBa_J61210/annotation1911675/range1911675/1
component: BBa_R0085

https://synbiohub.org/public/igem/BBa_J61210/annotation1911675/range1911675/1
type: Range
persistent identity: range1911675
display id: range1911675
version: 1
top level: BBa_J61210
direction: direction/reverse
start: 1401
end: 1423
orientation: inline

BBa_J61022
type: SequenceAnnotation
persistent identity: annotation1911676
display id: annotation1911676
version: 1
top level: BBa_J61210
location: https://synbiohub.org/public/igem/BBa_J61210/annotation1911676/range1911676/1
component: BBa_J61022

https://synbiohub.org/public/igem/BBa_J61210/annotation1911676/range1911676/1
type: Range
persistent identity: range1911676
display id: range1911676
version: 1
top level: BBa_J61210
direction: direction/reverse
start: 1432
end: 1450
orientation: inline

Tn5F
type: ComponentDefinition
persistent identity: BBa J61021
display id: BBa_J61021
version: 1
was generated by: iGEM to SBOL conversion
description: [Tn5-for]
created: 2006-09-20T11:00:00Z
modified: 2015-08-31T02:02:59Z
mutable provenance: Overlap extension of synthetic oligonucleotides
owned by: james
owned by: myers
mutable description: DNA substrate for Tn5 transposase. Flanking a sequence with J61021 on the 5' end and J61022 on the 3' end makes it a functional transposon.
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: John Anderson
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_J61021_sequence/1

Tn5R
type: ComponentDefinition
persistent identity: BBa J61022
display id: BBa_J61022
version: 1
was generated by: iGEM to SBOL conversion
description: [Tn5-rev]
created: 2006-09-20T11:00:00Z
modified: 2015-08-31T02:02:59Z
mutable provenance: Overlap extension of synthetic oligonucleotides
owned by: james
owned by: myers
mutable description: DNA substrate for Tn5 transposase. Flanking a sequence with J61021 on the 5' end and J61022 on the 3' end makes it a functional transposon. pSB1A2-Bca1013
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: John Anderson
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_J61022_sequence/1

rbsGenR
type: ComponentDefinition
persistent identity: BBa J61201
display id: BBa_J61201
version: 1
was generated by: iGEM to SBOL conversion
description: [rbsGenR]
created: 2007-01-30T12:00:00Z
modified: 2015-08-31T01:56:25Z
mutable provenance: (to be filled in)
owned by: james
owned by: myers
mutable description: Gentamicin resistance gene including a medium/weak ribosome binding site, but NO promoter. Intended for use in transposons, to select for elements that fall inside of a transcribed region. The RBS does not appear to be strong enough for this purpose.
discontinued: false
dominant: false
experience: experience/Issues
group u list: _95_
m user id: 0
owner id: 947
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: (to be filled in)
star: true
creator: Josh Kittleson
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: GenR
sequence: https://synbiohub.org/public/igem/BBa_J61201_sequence/1

GenR
type: SequenceAnnotation
persistent identity: annotation1911576
display id: annotation1911576
version: 1
top level: rbsGenR
location: https://synbiohub.org/public/igem/BBa_J61201/annotation1911576/range1911576/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_J61201/annotation1911576/range1911576/1
type: Range
persistent identity: range1911576
display id: range1911576
version: 1
top level: rbsGenR
direction: direction/forward
start: 21
end: 554
orientation: inline

BBa_R0085
type: ComponentDefinition
persistent identity: BBa R0085
display id: BBa_R0085
version: 1
was generated by: iGEM to SBOL conversion
description: T7 Consensus Promoter Sequence
created: 2005-02-21T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: Sequence obtained from Sri Kosuri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The T7 promoter should only produce PoPS when the T7 polymerase is also being expressed.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Barry Canton
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: Polymerase Binding Region
sequence annotation: Initiation Region
sequence annotation: Transcription Start Site
sequence: https://synbiohub.org/public/igem/BBa_R0085_sequence/1

Polymerase Binding Region
type: SequenceAnnotation
persistent identity: annotation1721521
display id: annotation1721521
version: 1
top level: BBa_R0085
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721521/range1721521/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0085/annotation1721521/range1721521/1
type: Range
persistent identity: range1721521
display id: range1721521
version: 1
top level: BBa_R0085
direction: direction/forward
start: 1
end: 11
orientation: inline

Initiation Region
type: SequenceAnnotation
persistent identity: annotation1721522
display id: annotation1721522
version: 1
top level: BBa_R0085
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721522/range1721522/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_R0085/annotation1721522/range1721522/1
type: Range
persistent identity: range1721522
display id: range1721522
version: 1
top level: BBa_R0085
direction: direction/forward
start: 12
end: 23
orientation: inline

Transcription Start Site
type: SequenceAnnotation
persistent identity: annotation1721520
display id: annotation1721520
version: 1
top level: BBa_R0085
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721520/range1721520/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_R0085/annotation1721520/range1721520/1
type: Range
persistent identity: range1721520
display id: range1721520
version: 1
top level: BBa_R0085
direction: direction/forward
start: 18
end: 18
orientation: inline

CmR
type: ComponentDefinition
persistent identity: BBa J61000
display id: BBa_J61000
version: 1
was generated by: iGEM to SBOL conversion
description: chloramphenicol resistance cassette
created: 2006-07-30T11:00:00Z
modified: 2015-08-31T02:02:59Z
mutable provenance: PCR ca1016F/R on pKD3 (934 bp, EcoRI/SpeI) Sub into pSB1A2-I13522 (EcoRI/SpeI) Products are Bca1015 and Bca1016 ---- ca1016F Forward EcoRI to biobrick CAT of pKD3 gacttgaattcgcggccgcttctagaggaataggaacttcatttaaatg ca1016R Reverse SpeI to biobrick CAT of pKD3 ccgctactagtggcgcgcctacctgtgacgg
owned by: james
owned by: myers
mutable description: Chloramphenicol resistance gene including its native promoter and ribosome binding site. Confers resistance to 25 ug/mL chloramphenicol. {pSB1A2-Bca1016}
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: CmR
sequence: https://synbiohub.org/public/igem/BBa_J61000_sequence/1

CmR
type: SequenceAnnotation
persistent identity: annotation1892270
display id: annotation1892270
version: 1
top level: CmR
location: https://synbiohub.org/public/igem/BBa_J61000/annotation1892270/range1892270/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J61000/annotation1892270/range1892270/1
type: Range
persistent identity: range1892270
display id: range1892270
version: 1
top level: CmR
direction: direction/reverse
start: 30
end: 687
orientation: inline

R6K
type: ComponentDefinition
persistent identity: BBa J61001
display id: BBa_J61001
version: 1
was generated by: iGEM to SBOL conversion
description: [R6K] Origin of replication
created: 2006-07-30T11:00:00Z
modified: 2015-08-31T02:02:59Z
mutable provenance: PCR ca1011F/R on pG80ko (445 bp, EcoRI/SpeI) Sub into pSB1A2-I13522 (EcoRI/SpeI) Product is Bca1011 ---- ca1011F Forward Biobricking of R6K GGACTgaattcgcggccgcttctagagtgattcgcacgggcccatg ca1011R Reverse biobricking of R6K ccgctactagtaGCAGTTCAACCTGTTGATAG
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: R6K origin of replication requires a pir+ or pir116 strain for replication in the absence of a second origin. In most E. coli strains, this is a silent feature. {pSB1A2-Bca1011}
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: R6K
sequence: https://synbiohub.org/public/igem/BBa_J61001_sequence/1

R6K
type: SequenceAnnotation
persistent identity: annotation1892271
display id: annotation1892271
version: 1
top level: R6K
location: https://synbiohub.org/public/igem/BBa_J61001/annotation1892271/range1892271/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J61001/annotation1892271/range1892271/1
type: Range
persistent identity: range1892271
display id: range1892271
version: 1
top level: R6K
direction: direction/forward
start: 27
end: 406
orientation: inline

FRT
type: ComponentDefinition
persistent identity: BBa J61020
display id: BBa_J61020
version: 1
was generated by: iGEM to SBOL conversion
description: [FRT]
created: 2006-09-20T11:00:00Z
modified: 2015-08-31T02:02:59Z
mutable provenance: Extend ca1010F/R (73 bp, EcoRI/SpeI) Sub into pSB1A2-I13522 (EcoRI/SpeI) Product is pSB1A2-Bca1010 ---- ca1010F Forward (universal biobrick) EcoRI for FRT GGACTgaattcgcggccgcttctagag ca1010R Reverse SpeI oligo for FRT cctatactagtagaagttcctattctctaAaaagtataggaacttcctctagaagcggccgcg
owned by: james
owned by: myers
mutable description: Site for recombination by flp recombinase. Genes flanked by FRT sites (oriented in the same direction) in the genome can be excised with the introduction of flp recombinase-expressing helper plasmid pCP20. Note that the original FRT sequence contained an XbaI site that has been removed with a point mutation for compatibility with standard assembly. See Datsenko and Wanner for details of its use in markerless knockouts and knockins.
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: John Anderson
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_J61020_sequence/1

https://synbiohub.org/public/igem/BBa_J61211_sequence/1
type: Sequence
persistent identity: BBa J61211 sequence
display id: BBa_J61211_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgtctcttatacacatcttactagaggatccgttccggaatgtttgatgttatggagcagcaacgatgttacgcagcagggcagtcgccctaaaacaaagttaggtggctcaagtatgggcatcattcgcacatgtaggctcggccctgaccaagtcaaatccatgcgggctgctcttgatcttttcggtcgtgagttcggagacgtagccacctactcccaacatcagccggactccgattacctcgggaacttgctccgtagtaagacattcatcgcgcttgctgccttcgaccaagaagcggttgttggcgctctcgcggcttacgttctgcccaagtttgagcagccgcgtagtgagatctatatctatgatctcgcagtctccggagagcaccggaggcagggcattgccaccgcgctcatcaatctcctcaagcatgaggccaacgcgcttggtgcttatgtgatctacgtgcaagcagattacggtgacgatcccgcagtggctctctatacaaagttgggcatacgggaagaagtgatgcactttgatatcgacccaagtaccgccacctaacgaatttactagaggaagttcctatactttttagagaataggaacttctactagagtgattcgcacgggcccatggctaattcccatgtcagccgttaagtgttcctgtgtcactcaaaattgctttgagaggctctaagggcttctcagtgcgttacatccctggcttgttgtccacaaccgttaaaccttaaaagctttaaaagccttatatattcttttttttcttataaaacttaaaaccttagaggctatttaagttgctgatttatattaattttattgttcaaacatgagagcttagtacgtgaaacatgagagcttagtacgttagccatgagagcttagtacgttagccatgagggtttagttcgttaaacatgagagcttagtacgttaaacatgagagcttagtacgtgaaacatgagagcttagtacgtactatcaacaggttgaactgctactagaggaataggaacttcatttaaatggcgcgccttacgccccgccctgccactcatcgcagtactgttgtattcattaagcatctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgtaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgacaactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgccgatcaacgtctcattttcgccaaaagttggcccagggcttcccggtatcaacagggacaccaggatttatttattctgcgaagtgatcttccgtcacaggtaggcgcgctactagaggaagttcctatactttttagagaataggaacttctactagagtaata…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61202_sequence/1
type: Sequence
persistent identity: BBa J61202 sequence
display id: BBa_J61202_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgtctcttatacacatcttactagaggatccgttccggaatgtttgatgttatggagcagcaacgatgttacgcagcagggcagtcgccctaaaacaaagttaggtggctcaagtatgggcatcattcgcacatgtaggctcggccctgaccaagtcaaatccatgcgggctgctcttgatcttttcggtcgtgagttcggagacgtagccacctactcccaacatcagccggactccgattacctcgggaacttgctccgtagtaagacattcatcgcgcttgctgccttcgaccaagaagcggttgttggcgctctcgcggcttacgttctgcccaagtttgagcagccgcgtagtgagatctatatctatgatctcgcagtctccggagagcaccggaggcagggcattgccaccgcgctcatcaatctcctcaagcatgaggccaacgcgcttggtgcttatgtgatctacgtgcaagcagattacggtgacgatcccgcagtggctctctatacaaagttgggcatacgggaagaagtgatgcactttgatatcgacccaagtaccgccacctaacgaatt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61210_sequence/1
type: Sequence
persistent identity: BBa J61210 sequence
display id: BBa_J61210_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaagttcctatactttttagagaataggaacttctactagagtgattcgcacgggcccatggctaattcccatgtcagccgttaagtgttcctgtgtcactcaaaattgctttgagaggctctaagggcttctcagtgcgttacatccctggcttgttgtccacaaccgttaaaccttaaaagctttaaaagccttatatattcttttttttcttataaaacttaaaaccttagaggctatttaagttgctgatttatattaattttattgttcaaacatgagagcttagtacgtgaaacatgagagcttagtacgttagccatgagagcttagtacgttagccatgagggtttagttcgttaaacatgagagcttagtacgttaaacatgagagcttagtacgtgaaacatgagagcttagtacgtactatcaacaggttgaactgctactagaggaataggaacttcatttaaatggcgcgccttacgccccgccctgccactcatcgcagtactgttgtattcattaagcatctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgtaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgacaactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgccgatcaacgtctcattttcgccaaaagttggcccagggcttcccggtatcaacagggacaccaggatttatttattctgcgaagtgatcttccgtcacaggtaggcgcgctactagaggaagttcctatactttttagagaataggaacttctactagagtaatacgactcactatagggagatactagagagatgtgtataagagacag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61021_sequence/1
type: Sequence
persistent identity: BBa J61021 sequence
display id: BBa_J61021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgtctcttatacacatct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61022_sequence/1
type: Sequence
persistent identity: BBa J61022 sequence
display id: BBa_J61022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agatgtgtataagagacag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61201_sequence/1
type: Sequence
persistent identity: BBa J61201 sequence
display id: BBa_J61201_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gatccgttccggaatgtttgatgttatggagcagcaacgatgttacgcagcagggcagtcgccctaaaacaaagttaggtggctcaagtatgggcatcattcgcacatgtaggctcggccctgaccaagtcaaatccatgcgggctgctcttgatcttttcggtcgtgagttcggagacgtagccacctactcccaacatcagccggactccgattacctcgggaacttgctccgtagtaagacattcatcgcgcttgctgccttcgaccaagaagcggttgttggcgctctcgcggcttacgttctgcccaagtttgagcagccgcgtagtgagatctatatctatgatctcgcagtctccggagagcaccggaggcagggcattgccaccgcgctcatcaatctcctcaagcatgaggccaacgcgcttggtgcttatgtgatctacgtgcaagcagattacggtgacgatcccgcagtggctctctatacaaagttgggcatacgggaagaagtgatgcactttgatatcgacccaagtaccgccacctaacgaatt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0085_sequence/1
type: Sequence
persistent identity: BBa R0085 sequence
display id: BBa_R0085_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61000_sequence/1
type: Sequence
persistent identity: BBa J61000 sequence
display id: BBa_J61000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaataggaacttcatttaaatggcgcgccttacgccccgccctgccactcatcgcagtactgttgtattcattaagcatctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgtaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgacaactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgccgatcaacgtctcattttcgccaaaagttggcccagggcttcccggtatcaacagggacaccaggatttatttattctgcgaagtgatcttccgtcacaggtaggcgcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61001_sequence/1
type: Sequence
persistent identity: BBa J61001 sequence
display id: BBa_J61001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgattcgcacgggcccatggctaattcccatgtcagccgttaagtgttcctgtgtcactcaaaattgctttgagaggctctaagggcttctcagtgcgttacatccctggcttgttgtccacaaccgttaaaccttaaaagctttaaaagccttatatattcttttttttcttataaaacttaaaaccttagaggctatttaagttgctgatttatattaattttattgttcaaacatgagagcttagtacgtgaaacatgagagcttagtacgttagccatgagagcttagtacgttagccatgagggtttagttcgttaaacatgagagcttagtacgttaaacatgagagcttagtacgtgaaacatgagagcttagtacgtactatcaacaggttgaactgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61020_sequence/1
type: Sequence
persistent identity: BBa J61020 sequence
display id: BBa_J61020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaagttcctatactttttagagaataggaacttc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z