LOCUS BBa_J61212 5061 bp DNA linear 22-Dec-2024 DEFINITION Tn5F|rbsGenR|FRT|R6K|CmR|FRT|P_T7|Tn5R||SpecR|OriTr|TnP ACCESSION BBa_J61212 VERSION BBa_J61212.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=BBa_J61021 CDS 48..581 /label=GenR misc_feature 28..587 /label=BBa_J61201 misc_feature 1..587 /label=BBa_J61202 misc_feature 596..629 /label=BBa_J61020 misc_feature 664..1043 /label=R6K misc_feature 638..1043 /label=BBa_J61001 CDS 1081..1738 /label=CmR misc_feature 1052..1945 /label=BBa_J61000 misc_feature 1954..1987 /label=BBa_J61020 misc_feature 2013..2013 /label=Transcription Start Site misc_feature 1996..2006 /label=Polymerase Binding Region misc_feature 2007..2018 /label=Initiation Region promoter 1996..2018 /label=BBa_R0085 misc_feature 2027..2045 /label=BBa_J61022 misc_feature 596..2045 /label=BBa_J61210 misc_feature 1..2045 /label=BBa_J61211 CDS 2099..2890 /label=SpR Spectinomycin Resistance misc_feature 2054..2941 /label=BBa_J23012 oriT 2950..3320 /label=BBa_J01003 misc_feature 3329..5061 /label=BBa_J61023 misc_feature 2054..5061 /label=BBa_J61209 ORIGIN 1 ctgtctctta tacacatctt actagaggat ccgttccgga atgtttgatg ttatggagca 61 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaggtggc tcaagtatgg 121 gcatcattcg cacatgtagg ctcggccctg accaagtcaa atccatgcgg gctgctcttg 181 atcttttcgg tcgtgagttc ggagacgtag ccacctactc ccaacatcag ccggactccg 241 attacctcgg gaacttgctc cgtagtaaga cattcatcgc gcttgctgcc ttcgaccaag 301 aagcggttgt tggcgctctc gcggcttacg ttctgcccaa gtttgagcag ccgcgtagtg 361 agatctatat ctatgatctc gcagtctccg gagagcaccg gaggcagggc attgccaccg 421 cgctcatcaa tctcctcaag catgaggcca acgcgcttgg tgcttatgtg atctacgtgc 481 aagcagatta cggtgacgat cccgcagtgg ctctctatac aaagttgggc atacgggaag 541 aagtgatgca ctttgatatc gacccaagta ccgccaccta acgaatttac tagaggaagt 601 tcctatactt tttagagaat aggaacttct actagagtga ttcgcacggg cccatggcta 661 attcccatgt cagccgttaa gtgttcctgt gtcactcaaa attgctttga gaggctctaa 721 gggcttctca gtgcgttaca tccctggctt gttgtccaca accgttaaac cttaaaagct 781 ttaaaagcct tatatattct tttttttctt ataaaactta aaaccttaga ggctatttaa 841 gttgctgatt tatattaatt ttattgttca aacatgagag cttagtacgt gaaacatgag 901 agcttagtac gttagccatg agagcttagt acgttagcca tgagggttta gttcgttaaa 961 catgagagct tagtacgtta aacatgagag cttagtacgt gaaacatgag agcttagtac 1021 gtactatcaa caggttgaac tgctactaga ggaataggaa cttcatttaa atggcgcgcc 1081 ttacgccccg ccctgccact catcgcagta ctgttgtatt cattaagcat ctgccgacat 1141 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc 1201 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca 1261 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct 1321 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat 1381 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt 1441 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac 1501 cgtctttcat tgccatacgt aattccggat gagcattcat caggcgggca agaatgtgaa 1561 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat 1621 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt 1681 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt 1741 tagcttcctt agctcctgaa aatctcgaca actcaaaaaa tacgcccggt agtgatctta 1801 tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat tttcgccaaa 1861 agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat tctgcgaagt 1921 gatcttccgt cacaggtagg cgcgctacta gaggaagttc ctatactttt tagagaatag 1981 gaacttctac tagagtaata cgactcacta tagggagata ctagagagat gtgtataaga 2041 gacagtacta gagtccaagc gagctcgata tccgtcggct tgaacgaatt gttagacatt 2101 atttgccgac taccttggtg atctcgcctt tcacgtagtg gacaaattct tccaactgat 2161 ctgcgcgcga ggccaagcga tcttcttctt gtccaagata agcctgtcta gcttcaagta 2221 tgacgggctg atactgggcc ggcaggcgct ccattgccca gtcggcagcg acatccttcg 2281 gcgcgatttt gccggttact gcgctgtacc aaatgcggga caacgtaagc actacatttc 2341 gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt taaggtttca tttagcgcct 2401 caaatagatc ctgttcagga accggatcaa agagttcctc cgccgctgga cctaccaagg 2461 caacgctatg ttctcttgct tttgtcagca agatagccag atcaatgtcg tacgtggctg 2521 gctcgaagat acctgcaaga atgtcattgc gctgccattc tccaaattgc agttcgcgct 2581 tagctggata acgccacgga atgatgtcgt cgtgcacaac aatggtgact tctacagcgc 2641 ggagaatctc gctctctcca ggggaagccg aagtttccaa aaggtcgttg atcaaagctc 2701 gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag caaatcaata tcactgtgtg 2761 gcttcaggcc gccatccact gcggagccgt acaaatgtac ggccagcaac gtcggttcga 2821 gatggcgctc gatgacgcca actacctctg atagttgagt cgatacttcg gcgatcaccg 2881 cttccctcat gatgtttaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc 2941 ttactagagg ccgccttttc ctcaatcgct cttcgttcgt ctggaaggca gtacaccttg 3001 ataggtgggc tgcccttcct ggttggcttg gtttcatcag ccatccgctt gccctcatct 3061 gttacgccgg cggtagccgg ccagcctcgc agagcaggat tcccgttgag caccgccagg 3121 tgcgaataag ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc 3181 ctgcccggct gacgccgttg gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat 3241 cctgtatatc gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat gaccccgaag 3301 cagggttatg cagcggaaaa tactagagtg attctccgcc agcatggctt cggccagtgc 3361 gtcgagcagc gcccgcttgt tcctgaagtg ccagtaaagc gccggctgct gaacccccaa 3421 ccgttccgcc agtttgcgtg tcgtcagacc gtctacgccg acctcgttca acaggtctag 3481 ggcggcacgg atcactgtat tcggctgcaa ctttgtcatg cttgacactt tatcactgat 3541 aaacataata tgtccaccaa cttatcagtg ataaagaatc cgcgcgttca atcggaccag 3601 cggaggctgg tccggaggcc acatatgata acttctgctc ttcatcgtgc ggccgactgg 3661 gctaaatctg tgttctcttc ggcggcgctg ggtgatcctc gccgtactgc ccgcttggtt 3721 aacgtcgccg cccaattggc aaaatattct ggtaaatcaa taaccatctc atcagagggt 3781 agtaaagccg cccaggaagg cgcttaccga tttatccgca atcccaacgt ttctgccgag 3841 gcgatcagaa aggctggcgc catgcaaaca gtcaagttgg ctcaggagtt tcccgaactg 3901 ctggccattg aggacaccac ctctttgagt tatcgccacc aggtcgccga agagcttggc 3961 aagctgggct ctattcagga taaatcccgc ggatggtggg ttcactccgt tctcttgctc 4021 gaggccacca cattccgcac cgtaggatta ctgcatcagg agtggtggat gcgcccggat 4081 gaccctgccg atgcggatga aaaggagagt ggcaaatggc tggcagcggc cgcaactagc 4141 cggttacgca tgggcagcat gatgagcaac gtgattgcgg tctgtgaccg cgaagccgat 4201 attcatgctt atctgcagga caaactggcg cataacgagc gcttcgtggt gcgctccaag 4261 cacccacgca aggacgtaga gtctgggttg tatctgtacg accatctgaa gaaccaaccg 4321 gagttgggtg gctatcagat cagcattccg caaaagggcg tggtggataa acgcggtaaa 4381 cgtaaaaatc gaccagcccg caaggcgagc ttgagcctgc gcagtgggcg catcacgcta 4441 aaacagggga atatcacgct caacgcggtg ctggccgagg agattaaccc gcccaagggt 4501 gagaccccgt tgaaatggtt gttgctgacc agcgaaccgg tcgagtcgct agcccaagcc 4561 ttgcgcgtca tcgacattta tacccatcgc tggcggatcg aggagttcca taaggcatgg 4621 aaaaccggag caggagccga gaggcaacgc atggaggagc cggataatct ggagcggatg 4681 gtctcgatcc tctcgtttgt tgcggtcagg ctgttacagc tcagagaaag cttcacgccg 4741 ccgcaagcac tcagggcgca agggctgcta aaggaagcgg aacacgtaga aagccagtcc 4801 gcagaaacgg tgctgacccc ggatgaatgt cagctactgg gctatctgga caagggaaaa 4861 cgcaagcgca aagagaaagc aggtagcttg cagtgggctt acatggcgat agctagactg 4921 ggcggtttta tggacagcaa gcgaaccgga attgccagct ggggcgccct ctgggaaggt 4981 tgggaagccc tgcaaagtaa actggatggc tttcttgccg ccaaggatct gatggcgcag 5041 gggatcaaga tctgatccgt c //