BBa_J61212
type: ComponentDefinition
persistent identity: BBa J61212
display id: BBa_J61212
version: 1
was generated by: iGEM to SBOL conversion
description: Tn5F|rbsGenR|FRT|R6K|CmR|FRT|P_T7|Tn5R||SpecR|OriTr|TnP
created: 2007-01-31T12:00:00Z
modified: 2015-08-31T01:56:25Z
mutable provenance: see composite parts.
owned by: james
owned by: myers
mutable description: Transposon bearing chloramphenicol resistance on a suicide plasmid bearing spectinomycin resistance. The parts are not actually colinear, as in a normal composite assembly. The coding strand changes at the || indicated in the name.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _95_
m user id: 0
owner id: 947
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: no special considerations.
star: false
creator: Josh Kittleson
type: DnaRegion
role: SO:0000110
role: partType/Other
component: BBa_J61209
component: BBa_J61211
sequence annotation: BBa_J61211
sequence annotation: BBa_J61209
sequence: https://synbiohub.org/public/igem/BBa_J61212_sequence/1

BBa_J61209
type: Component
persistent identity: component2232000
display id: component2232000
version: 1
top level: BBa_J61212
definition: BBa_J61209
access: public

BBa_J61211
type: Component
persistent identity: component2231995
display id: component2231995
version: 1
top level: BBa_J61212
definition: BBa_J61211
access: public

BBa_J61211
type: SequenceAnnotation
persistent identity: annotation2231995
display id: annotation2231995
version: 1
top level: BBa_J61212
location: https://synbiohub.org/public/igem/BBa_J61212/annotation2231995/range2231995/1
component: BBa_J61211

https://synbiohub.org/public/igem/BBa_J61212/annotation2231995/range2231995/1
type: Range
persistent identity: range2231995
display id: range2231995
version: 1
top level: BBa_J61212
direction: direction/reverse
start: 1
end: 2045
orientation: inline

BBa_J61209
type: SequenceAnnotation
persistent identity: annotation2232000
display id: annotation2232000
version: 1
top level: BBa_J61212
location: https://synbiohub.org/public/igem/BBa_J61212/annotation2232000/range2232000/1
component: BBa_J61209

https://synbiohub.org/public/igem/BBa_J61212/annotation2232000/range2232000/1
type: Range
persistent identity: range2232000
display id: range2232000
version: 1
top level: BBa_J61212
direction: direction/reverse
start: 2054
end: 5061
orientation: inline

BBa_J61209
type: ComponentDefinition
persistent identity: BBa J61209
display id: BBa_J61209
version: 1
was generated by: iGEM to SBOL conversion
description: [SpecR][OriTr][TnP]
created: 2007-01-31T12:00:00Z
modified: 2015-08-31T01:56:25Z
mutable provenance: see composite parts.
owned by: james
owned by: myers
mutable description: Intermediate for construction of a transposon with a plasmid bearing a spectinomycin resistance marker.
discontinued: false
dominant: false
experience: experience/None
group u list: _95_
m user id: 0
owner id: 947
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: no special design considerations.
star: false
creator: Josh Kittleson
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_J61023
component: BBa_J01003
component: BBa_J23012
sequence annotation: BBa_J23012
sequence annotation: BBa_J01003
sequence annotation: BBa_J61023
sequence: https://synbiohub.org/public/igem/BBa_J61209_sequence/1

BBa_J61023
type: Component
persistent identity: component1911665
display id: component1911665
version: 1
top level: BBa_J61209
definition: TnP
access: public

BBa_J01003
type: Component
persistent identity: component1911664
display id: component1911664
version: 1
top level: BBa_J61209
definition: OriTR
access: public

BBa_J23012
type: Component
persistent identity: component1911663
display id: component1911663
version: 1
top level: BBa_J61209
definition: BBa_J23012
access: public

BBa_J23012
type: SequenceAnnotation
persistent identity: annotation1911663
display id: annotation1911663
version: 1
top level: BBa_J61209
location: https://synbiohub.org/public/igem/BBa_J61209/annotation1911663/range1911663/1
component: BBa_J23012

https://synbiohub.org/public/igem/BBa_J61209/annotation1911663/range1911663/1
type: Range
persistent identity: range1911663
display id: range1911663
version: 1
top level: BBa_J61209
direction: direction/reverse
start: 1
end: 888
orientation: inline

BBa_J01003
type: SequenceAnnotation
persistent identity: annotation1911664
display id: annotation1911664
version: 1
top level: BBa_J61209
location: https://synbiohub.org/public/igem/BBa_J61209/annotation1911664/range1911664/1
component: BBa_J01003

https://synbiohub.org/public/igem/BBa_J61209/annotation1911664/range1911664/1
type: Range
persistent identity: range1911664
display id: range1911664
version: 1
top level: BBa_J61209
direction: direction/reverse
start: 897
end: 1267
orientation: inline

BBa_J61023
type: SequenceAnnotation
persistent identity: annotation1911665
display id: annotation1911665
version: 1
top level: BBa_J61209
location: https://synbiohub.org/public/igem/BBa_J61209/annotation1911665/range1911665/1
component: BBa_J61023

https://synbiohub.org/public/igem/BBa_J61209/annotation1911665/range1911665/1
type: Range
persistent identity: range1911665
display id: range1911665
version: 1
top level: BBa_J61209
direction: direction/reverse
start: 1276
end: 3008
orientation: inline

BBa_J61211
type: ComponentDefinition
persistent identity: BBa J61211
display id: BBa_J61211
version: 1
was generated by: iGEM to SBOL conversion
description: [Tn5F][rbsGenR][FRT][R6K][CmR][FRT][P_T7][Tn5R]
created: 2007-01-31T12:00:00Z
modified: 2015-08-31T01:56:25Z
mutable provenance: see composite parts.
owned by: james
owned by: myers
mutable description: Transposable piece of a transposon bearing suicide plasmid, containing chloramphenicol resistance, an origin of replication for decoding, and a selectable gentimicin marker that only activates when the transposon lands in a transcribed region.
discontinued: false
dominant: false
experience: experience/None
group u list: _95_
m user id: 0
owner id: 947
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: no special considerations.
star: false
creator: Josh Kittleson
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J61210
component: BBa_J61202
sequence annotation: BBa_J61202
sequence annotation: BBa_J61210
sequence: https://synbiohub.org/public/igem/BBa_J61211_sequence/1

BBa_J61210
type: Component
persistent identity: component2226370
display id: component2226370
version: 1
top level: BBa_J61211
definition: BBa_J61210
access: public

BBa_J61202
type: Component
persistent identity: component2226358
display id: component2226358
version: 1
top level: BBa_J61211
definition: BBa_J61202
access: public

BBa_J61202
type: SequenceAnnotation
persistent identity: annotation2226358
display id: annotation2226358
version: 1
top level: BBa_J61211
location: https://synbiohub.org/public/igem/BBa_J61211/annotation2226358/range2226358/1
component: BBa_J61202

https://synbiohub.org/public/igem/BBa_J61211/annotation2226358/range2226358/1
type: Range
persistent identity: range2226358
display id: range2226358
version: 1
top level: BBa_J61211
direction: direction/reverse
start: 1
end: 587
orientation: inline

BBa_J61210
type: SequenceAnnotation
persistent identity: annotation2226370
display id: annotation2226370
version: 1
top level: BBa_J61211
location: https://synbiohub.org/public/igem/BBa_J61211/annotation2226370/range2226370/1
component: BBa_J61210

https://synbiohub.org/public/igem/BBa_J61211/annotation2226370/range2226370/1
type: Range
persistent identity: range2226370
display id: range2226370
version: 1
top level: BBa_J61211
direction: direction/reverse
start: 596
end: 2045
orientation: inline

BBa_J23012
type: ComponentDefinition
persistent identity: BBa J23012
display id: BBa_J23012
version: 1
was generated by: iGEM to SBOL conversion
description: Spectinomycin R open-reading-frame basic part
created: 2006-07-23T11:00:00Z
modified: 2015-08-31T04:08:38Z
mutable provenance: PCR JL1/JL2 on wt. pSPlam (917bp, EcoRI/SpeI, Neb2) Paste into pSB1A2-I13521 (EcoRI/SpeI, Neb2, Large 2062)
owned by: james
owned by: myers
mutable description: it is a Spec resistant biobrick
discontinued: false
dominant: false
experience: experience/Issues
group u list: _52_
m user id: 0
owner id: 363
owning group id: 52
sample status: In stock
status: status/Available
bookmark: false
mutable notes: JL1 Forward EcoRI oligo to bioBrick SpecR: gacttgaattcgcggccgcttctagagtccaagcgagctcgatatc JL2 Reverse SpeI oligo to bioBrick SpecR: ccgctactagtCAGCAACGATGTTACGCAGC
star: false
creator: Will Bosworth
type: DnaRegion
role: partType/Generator
role: SO:0000804
sequence annotation: SpR Spectinomycin Resistance
sequence: https://synbiohub.org/public/igem/BBa_J23012_sequence/1

SpR Spectinomycin Resistance
type: SequenceAnnotation
persistent identity: annotation1966465
display id: annotation1966465
version: 1
top level: BBa_J23012
location: https://synbiohub.org/public/igem/BBa_J23012/annotation1966465/range1966465/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J23012/annotation1966465/range1966465/1
type: Range
persistent identity: range1966465
display id: range1966465
version: 1
top level: BBa_J23012
direction: direction/reverse
start: 46
end: 837
orientation: inline

TnP
type: ComponentDefinition
persistent identity: BBa J61023
display id: BBa_J61023
version: 1
was generated by: iGEM to SBOL conversion
description: Tn5 Transposase [Tnp1-PN] (with PstI and NotI)
created: 2006-09-20T11:00:00Z
modified: 2015-08-31T02:02:59Z
mutable provenance: PCR ca1014F/R on pRL27 (1763 bp, EcoRI/SpeI) Sub into pSB1A2-I13521 (EcoRI/SpeI) Product is Bca1014 *internal PstI and NotI sites
owned by: james
owned by: myers
mutable description: Tn5 transposase-expressing cassette from pRL. Cassette includes a complete promoter/rbs/orf cassette. The promoter is Ptet, but not derived from r0040. There are PstI and NotI sites in this part, so care must be taken when doing assembly not to use those sites. pSB1A2-Bca1014
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: There are PstI and NotI sites in this part, so care must be taken when doing assembly not to use those sites.
star: true
creator: John Anderson
type: DnaRegion
role: partType/Generator
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_J61023_sequence/1

BBa_J61202
type: ComponentDefinition
persistent identity: BBa J61202
display id: BBa_J61202
version: 1
was generated by: iGEM to SBOL conversion
description: [Tn5F][rbsGenR]
created: 2007-01-30T12:00:00Z
modified: 2015-08-31T01:56:25Z
mutable provenance: See component parts.
owned by: james
owned by: myers
mutable description: Construction intermediate for a transposon selectable for insertion into a transcribed region.
discontinued: false
dominant: false
experience: experience/None
group u list: _95_
m user id: 0
owner id: 947
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: No special design considerations.
star: false
creator: Josh Kittleson
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_J61201
component: BBa_J61021
sequence annotation: BBa_J61021
sequence annotation: BBa_J61201
sequence: https://synbiohub.org/public/igem/BBa_J61202_sequence/1

BBa_J61201
type: Component
persistent identity: component2218368
display id: component2218368
version: 1
top level: BBa_J61202
definition: rbsGenR
access: public

BBa_J61021
type: Component
persistent identity: component2218366
display id: component2218366
version: 1
top level: BBa_J61202
definition: Tn5F
access: public

BBa_J61021
type: SequenceAnnotation
persistent identity: annotation2218366
display id: annotation2218366
version: 1
top level: BBa_J61202
location: https://synbiohub.org/public/igem/BBa_J61202/annotation2218366/range2218366/1
component: BBa_J61021

https://synbiohub.org/public/igem/BBa_J61202/annotation2218366/range2218366/1
type: Range
persistent identity: range2218366
display id: range2218366
version: 1
top level: BBa_J61202
direction: direction/reverse
start: 1
end: 19
orientation: inline

BBa_J61201
type: SequenceAnnotation
persistent identity: annotation2218368
display id: annotation2218368
version: 1
top level: BBa_J61202
location: https://synbiohub.org/public/igem/BBa_J61202/annotation2218368/range2218368/1
component: BBa_J61201

https://synbiohub.org/public/igem/BBa_J61202/annotation2218368/range2218368/1
type: Range
persistent identity: range2218368
display id: range2218368
version: 1
top level: BBa_J61202
direction: direction/reverse
start: 28
end: 587
orientation: inline

BBa_J61210
type: ComponentDefinition
persistent identity: BBa J61210
display id: BBa_J61210
version: 1
was generated by: iGEM to SBOL conversion
description: [FRT][R6K][CmR][FRT][P_T7][Tn5R]
created: 2007-01-31T12:00:00Z
modified: 2015-08-31T01:56:25Z
mutable provenance: see composite parts.
owned by: james
owned by: myers
mutable description: Intermediate for a transposon with a chloramphenicol resistance marker and a replication origin inside of the transposed region.
discontinued: false
dominant: false
experience: experience/None
group u list: _95_
m user id: 0
owner id: 947
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: no special design considerations
star: false
creator: Josh Kittleson
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J61022
component: BBa_J61020
component: BBa_R0085
component: BBa_J61000
component: BBa_J61001
sequence annotation: BBa_J61020
sequence annotation: BBa_J61001
sequence annotation: BBa_J61000
sequence annotation: BBa_R0085
sequence annotation: BBa_J61022
sequence: https://synbiohub.org/public/igem/BBa_J61210_sequence/1

BBa_J61022
type: Component
persistent identity: component1911676
display id: component1911676
version: 1
top level: BBa_J61210
definition: Tn5R
access: public

BBa_J61020
type: Component
persistent identity: component1911666
display id: component1911666
version: 1
top level: BBa_J61210
definition: FRT
access: public

BBa_R0085
type: Component
persistent identity: component1911675
display id: component1911675
version: 1
top level: BBa_J61210
definition: BBa_R0085
access: public

BBa_J61020
type: Component
persistent identity: component1911671
display id: component1911671
version: 1
top level: BBa_J61210
definition: FRT
access: public

BBa_J61000
type: Component
persistent identity: component1911670
display id: component1911670
version: 1
top level: BBa_J61210
definition: CmR
access: public

BBa_J61001
type: Component
persistent identity: component1911668
display id: component1911668
version: 1
top level: BBa_J61210
definition: R6K
access: public

BBa_J61020
type: SequenceAnnotation
persistent identity: annotation1911666
display id: annotation1911666
version: 1
top level: BBa_J61210
location: https://synbiohub.org/public/igem/BBa_J61210/annotation1911666/range1911666/1
component: BBa_J61020

https://synbiohub.org/public/igem/BBa_J61210/annotation1911666/range1911666/1
type: Range
persistent identity: range1911666
display id: range1911666
version: 1
top level: BBa_J61210
direction: direction/reverse
start: 1
end: 34
orientation: inline

BBa_J61001
type: SequenceAnnotation
persistent identity: annotation1911668
display id: annotation1911668
version: 1
top level: BBa_J61210
location: https://synbiohub.org/public/igem/BBa_J61210/annotation1911668/range1911668/1
component: BBa_J61001

https://synbiohub.org/public/igem/BBa_J61210/annotation1911668/range1911668/1
type: Range
persistent identity: range1911668
display id: range1911668
version: 1
top level: BBa_J61210
direction: direction/reverse
start: 43
end: 448
orientation: inline

BBa_J61000
type: SequenceAnnotation
persistent identity: annotation1911670
display id: annotation1911670
version: 1
top level: BBa_J61210
location: https://synbiohub.org/public/igem/BBa_J61210/annotation1911670/range1911670/1
component: BBa_J61000

https://synbiohub.org/public/igem/BBa_J61210/annotation1911670/range1911670/1
type: Range
persistent identity: range1911670
display id: range1911670
version: 1
top level: BBa_J61210
direction: direction/reverse
start: 457
end: 1350
orientation: inline

BBa_J61020
type: SequenceAnnotation
persistent identity: annotation1911671
display id: annotation1911671
version: 1
top level: BBa_J61210
location: https://synbiohub.org/public/igem/BBa_J61210/annotation1911671/range1911671/1
component: BBa_J61020

https://synbiohub.org/public/igem/BBa_J61210/annotation1911671/range1911671/1
type: Range
persistent identity: range1911671
display id: range1911671
version: 1
top level: BBa_J61210
direction: direction/reverse
start: 1359
end: 1392
orientation: inline

BBa_R0085
type: SequenceAnnotation
persistent identity: annotation1911675
display id: annotation1911675
version: 1
top level: BBa_J61210
location: https://synbiohub.org/public/igem/BBa_J61210/annotation1911675/range1911675/1
component: BBa_R0085

https://synbiohub.org/public/igem/BBa_J61210/annotation1911675/range1911675/1
type: Range
persistent identity: range1911675
display id: range1911675
version: 1
top level: BBa_J61210
direction: direction/reverse
start: 1401
end: 1423
orientation: inline

BBa_J61022
type: SequenceAnnotation
persistent identity: annotation1911676
display id: annotation1911676
version: 1
top level: BBa_J61210
location: https://synbiohub.org/public/igem/BBa_J61210/annotation1911676/range1911676/1
component: BBa_J61022

https://synbiohub.org/public/igem/BBa_J61210/annotation1911676/range1911676/1
type: Range
persistent identity: range1911676
display id: range1911676
version: 1
top level: BBa_J61210
direction: direction/reverse
start: 1432
end: 1450
orientation: inline

OriTR
type: ComponentDefinition
persistent identity: BBa J01003
display id: BBa_J01003
version: 1
was generated by: iGEM to SBOL conversion
description: OriT-R (Origin of transfer for the R-plasmid nic region)
created: 2005-10-17T11:00:00Z
modified: 2015-08-31T04:08:11Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: OriTR, the R plasmid nic region, is where the relaxosome nicks the plasmid and conjugative transfer by R plasmid machinery begins.
discontinued: false
dominant: false
experience: experience/None
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Golden Bear
type: DnaRegion
role: partType/Conjugation
role: SO:0000724
sequence: https://synbiohub.org/public/igem/BBa_J01003_sequence/1

Tn5F
type: ComponentDefinition
persistent identity: BBa J61021
display id: BBa_J61021
version: 1
was generated by: iGEM to SBOL conversion
description: [Tn5-for]
created: 2006-09-20T11:00:00Z
modified: 2015-08-31T02:02:59Z
mutable provenance: Overlap extension of synthetic oligonucleotides
owned by: james
owned by: myers
mutable description: DNA substrate for Tn5 transposase. Flanking a sequence with J61021 on the 5' end and J61022 on the 3' end makes it a functional transposon.
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: John Anderson
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_J61021_sequence/1

Tn5R
type: ComponentDefinition
persistent identity: BBa J61022
display id: BBa_J61022
version: 1
was generated by: iGEM to SBOL conversion
description: [Tn5-rev]
created: 2006-09-20T11:00:00Z
modified: 2015-08-31T02:02:59Z
mutable provenance: Overlap extension of synthetic oligonucleotides
owned by: james
owned by: myers
mutable description: DNA substrate for Tn5 transposase. Flanking a sequence with J61021 on the 5' end and J61022 on the 3' end makes it a functional transposon. pSB1A2-Bca1013
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: John Anderson
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_J61022_sequence/1

rbsGenR
type: ComponentDefinition
persistent identity: BBa J61201
display id: BBa_J61201
version: 1
was generated by: iGEM to SBOL conversion
description: [rbsGenR]
created: 2007-01-30T12:00:00Z
modified: 2015-08-31T01:56:25Z
mutable provenance: (to be filled in)
owned by: james
owned by: myers
mutable description: Gentamicin resistance gene including a medium/weak ribosome binding site, but NO promoter. Intended for use in transposons, to select for elements that fall inside of a transcribed region. The RBS does not appear to be strong enough for this purpose.
discontinued: false
dominant: false
experience: experience/Issues
group u list: _95_
m user id: 0
owner id: 947
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: (to be filled in)
star: true
creator: Josh Kittleson
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: GenR
sequence: https://synbiohub.org/public/igem/BBa_J61201_sequence/1

GenR
type: SequenceAnnotation
persistent identity: annotation1911576
display id: annotation1911576
version: 1
top level: rbsGenR
location: https://synbiohub.org/public/igem/BBa_J61201/annotation1911576/range1911576/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_J61201/annotation1911576/range1911576/1
type: Range
persistent identity: range1911576
display id: range1911576
version: 1
top level: rbsGenR
direction: direction/forward
start: 21
end: 554
orientation: inline

BBa_R0085
type: ComponentDefinition
persistent identity: BBa R0085
display id: BBa_R0085
version: 1
was generated by: iGEM to SBOL conversion
description: T7 Consensus Promoter Sequence
created: 2005-02-21T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: Sequence obtained from Sri Kosuri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The T7 promoter should only produce PoPS when the T7 polymerase is also being expressed.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Barry Canton
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: Polymerase Binding Region
sequence annotation: Initiation Region
sequence annotation: Transcription Start Site
sequence: https://synbiohub.org/public/igem/BBa_R0085_sequence/1

Polymerase Binding Region
type: SequenceAnnotation
persistent identity: annotation1721521
display id: annotation1721521
version: 1
top level: BBa_R0085
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721521/range1721521/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0085/annotation1721521/range1721521/1
type: Range
persistent identity: range1721521
display id: range1721521
version: 1
top level: BBa_R0085
direction: direction/forward
start: 1
end: 11
orientation: inline

Initiation Region
type: SequenceAnnotation
persistent identity: annotation1721522
display id: annotation1721522
version: 1
top level: BBa_R0085
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721522/range1721522/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_R0085/annotation1721522/range1721522/1
type: Range
persistent identity: range1721522
display id: range1721522
version: 1
top level: BBa_R0085
direction: direction/forward
start: 12
end: 23
orientation: inline

Transcription Start Site
type: SequenceAnnotation
persistent identity: annotation1721520
display id: annotation1721520
version: 1
top level: BBa_R0085
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721520/range1721520/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_R0085/annotation1721520/range1721520/1
type: Range
persistent identity: range1721520
display id: range1721520
version: 1
top level: BBa_R0085
direction: direction/forward
start: 18
end: 18
orientation: inline

CmR
type: ComponentDefinition
persistent identity: BBa J61000
display id: BBa_J61000
version: 1
was generated by: iGEM to SBOL conversion
description: chloramphenicol resistance cassette
created: 2006-07-30T11:00:00Z
modified: 2015-08-31T02:02:59Z
mutable provenance: PCR ca1016F/R on pKD3 (934 bp, EcoRI/SpeI) Sub into pSB1A2-I13522 (EcoRI/SpeI) Products are Bca1015 and Bca1016 ---- ca1016F Forward EcoRI to biobrick CAT of pKD3 gacttgaattcgcggccgcttctagaggaataggaacttcatttaaatg ca1016R Reverse SpeI to biobrick CAT of pKD3 ccgctactagtggcgcgcctacctgtgacgg
owned by: james
owned by: myers
mutable description: Chloramphenicol resistance gene including its native promoter and ribosome binding site. Confers resistance to 25 ug/mL chloramphenicol. {pSB1A2-Bca1016}
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: CmR
sequence: https://synbiohub.org/public/igem/BBa_J61000_sequence/1

CmR
type: SequenceAnnotation
persistent identity: annotation1892270
display id: annotation1892270
version: 1
top level: CmR
location: https://synbiohub.org/public/igem/BBa_J61000/annotation1892270/range1892270/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J61000/annotation1892270/range1892270/1
type: Range
persistent identity: range1892270
display id: range1892270
version: 1
top level: CmR
direction: direction/reverse
start: 30
end: 687
orientation: inline

R6K
type: ComponentDefinition
persistent identity: BBa J61001
display id: BBa_J61001
version: 1
was generated by: iGEM to SBOL conversion
description: [R6K] Origin of replication
created: 2006-07-30T11:00:00Z
modified: 2015-08-31T02:02:59Z
mutable provenance: PCR ca1011F/R on pG80ko (445 bp, EcoRI/SpeI) Sub into pSB1A2-I13522 (EcoRI/SpeI) Product is Bca1011 ---- ca1011F Forward Biobricking of R6K GGACTgaattcgcggccgcttctagagtgattcgcacgggcccatg ca1011R Reverse biobricking of R6K ccgctactagtaGCAGTTCAACCTGTTGATAG
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: R6K origin of replication requires a pir+ or pir116 strain for replication in the absence of a second origin. In most E. coli strains, this is a silent feature. {pSB1A2-Bca1011}
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: R6K
sequence: https://synbiohub.org/public/igem/BBa_J61001_sequence/1

R6K
type: SequenceAnnotation
persistent identity: annotation1892271
display id: annotation1892271
version: 1
top level: R6K
location: https://synbiohub.org/public/igem/BBa_J61001/annotation1892271/range1892271/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J61001/annotation1892271/range1892271/1
type: Range
persistent identity: range1892271
display id: range1892271
version: 1
top level: R6K
direction: direction/forward
start: 27
end: 406
orientation: inline

FRT
type: ComponentDefinition
persistent identity: BBa J61020
display id: BBa_J61020
version: 1
was generated by: iGEM to SBOL conversion
description: [FRT]
created: 2006-09-20T11:00:00Z
modified: 2015-08-31T02:02:59Z
mutable provenance: Extend ca1010F/R (73 bp, EcoRI/SpeI) Sub into pSB1A2-I13522 (EcoRI/SpeI) Product is pSB1A2-Bca1010 ---- ca1010F Forward (universal biobrick) EcoRI for FRT GGACTgaattcgcggccgcttctagag ca1010R Reverse SpeI oligo for FRT cctatactagtagaagttcctattctctaAaaagtataggaacttcctctagaagcggccgcg
owned by: james
owned by: myers
mutable description: Site for recombination by flp recombinase. Genes flanked by FRT sites (oriented in the same direction) in the genome can be excised with the introduction of flp recombinase-expressing helper plasmid pCP20. Note that the original FRT sequence contained an XbaI site that has been removed with a point mutation for compatibility with standard assembly. See Datsenko and Wanner for details of its use in markerless knockouts and knockins.
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: John Anderson
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_J61020_sequence/1

https://synbiohub.org/public/igem/BBa_J61212_sequence/1
type: Sequence
persistent identity: BBa J61212 sequence
display id: BBa_J61212_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgtctcttatacacatcttactagaggatccgttccggaatgtttgatgttatggagcagcaacgatgttacgcagcagggcagtcgccctaaaacaaagttaggtggctcaagtatgggcatcattcgcacatgtaggctcggccctgaccaagtcaaatccatgcgggctgctcttgatcttttcggtcgtgagttcggagacgtagccacctactcccaacatcagccggactccgattacctcgggaacttgctccgtagtaagacattcatcgcgcttgctgccttcgaccaagaagcggttgttggcgctctcgcggcttacgttctgcccaagtttgagcagccgcgtagtgagatctatatctatgatctcgcagtctccggagagcaccggaggcagggcattgccaccgcgctcatcaatctcctcaagcatgaggccaacgcgcttggtgcttatgtgatctacgtgcaagcagattacggtgacgatcccgcagtggctctctatacaaagttgggcatacgggaagaagtgatgcactttgatatcgacccaagtaccgccacctaacgaatttactagaggaagttcctatactttttagagaataggaacttctactagagtgattcgcacgggcccatggctaattcccatgtcagccgttaagtgttcctgtgtcactcaaaattgctttgagaggctctaagggcttctcagtgcgttacatccctggcttgttgtccacaaccgttaaaccttaaaagctttaaaagccttatatattcttttttttcttataaaacttaaaaccttagaggctatttaagttgctgatttatattaattttattgttcaaacatgagagcttagtacgtgaaacatgagagcttagtacgttagccatgagagcttagtacgttagccatgagggtttagttcgttaaacatgagagcttagtacgttaaacatgagagcttagtacgtgaaacatgagagcttagtacgtactatcaacaggttgaactgctactagaggaataggaacttcatttaaatggcgcgccttacgccccgccctgccactcatcgcagtactgttgtattcattaagcatctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgtaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgacaactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgccgatcaacgtctcattttcgccaaaagttggcccagggcttcccggtatcaacagggacaccaggatttatttattctgcgaagtgatcttccgtcacaggtaggcgcgctactagaggaagttcctatactttttagagaataggaacttctactagagtaata…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61209_sequence/1
type: Sequence
persistent identity: BBa J61209 sequence
display id: BBa_J61209_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccaagcgagctcgatatccgtcggcttgaacgaattgttagacattatttgccgactaccttggtgatctcgcctttcacgtagtggacaaattcttccaactgatctgcgcgcgaggccaagcgatcttcttcttgtccaagataagcctgtctagcttcaagtatgacgggctgatactgggccggcaggcgctccattgcccagtcggcagcgacatccttcggcgcgattttgccggttactgcgctgtaccaaatgcgggacaacgtaagcactacatttcgctcatcgccagcccagtcgggcggcgagttccatagcgttaaggtttcatttagcgcctcaaatagatcctgttcaggaaccggatcaaagagttcctccgccgctggacctaccaaggcaacgctatgttctcttgcttttgtcagcaagatagccagatcaatgtcgtacgtggctggctcgaagatacctgcaagaatgtcattgcgctgccattctccaaattgcagttcgcgcttagctggataacgccacggaatgatgtcgtcgtgcacaacaatggtgacttctacagcgcggagaatctcgctctctccaggggaagccgaagtttccaaaaggtcgttgatcaaagctcgccgcgttgtttcatcaagccttacggtcaccgtaaccagcaaatcaatatcactgtgtggcttcaggccgccatccactgcggagccgtacaaatgtacggccagcaacgtcggttcgagatggcgctcgatgacgccaactacctctgatagttgagtcgatacttcggcgatcaccgcttccctcatgatgtttaactttgttttagggcgactgccctgctgcgtaacatcgttgcttactagaggccgccttttcctcaatcgctcttcgttcgtctggaaggcagtacaccttgataggtgggctgcccttcctggttggcttggtttcatcagccatccgcttgccctcatctgttacgccggcggtagccggccagcctcgcagagcaggattcccgttgagcaccgccaggtgcgaataagggacagtgaagaaggaacacccgctcgcgggtgggcctacttcacctatcctgcccggctgacgccgttggatacaccaaggaaagtctacacgaaccctttggcaaaatcctgtatatcgtgcgaaaaaggatggatataccgaaaaaatcgctataatgaccccgaagcagggttatgcagcggaaaatactagagtgattctccgccagcatggcttcggccagtgcgtcgagcagcgcccgcttgttcctgaagtgccagtaaagcgccggctgctgaacccccaaccgttccgccagtttgcgtgtcgtcagaccgtctacgccgacctcgttcaacaggtctagggcggcacggatcactgtattcggctgcaactttgtcatgcttgacactttatcactgataaacataatatgtccaccaacttatcagtgataaagaatccgcgcgttcaatcggaccagcggaggctggtccggaggccacatatgataacttctgctcttcatcgtgcggccgactgggctaaatctgtgttctcttcggcggcgctgggtgatcctcgccgtactgcccgcttggttaacgtcgccgcccaattggcaaaatattctggtaaatcaataaccatctcatcagagggtagtaaagccgcccaggaaggcgcttaccgatttatccgcaatcccaacgtttctgccgaggcgatcagaaaggctggcgccatgcaaacagtcaagttggctcaggagtttcccgaactgctggccattgaggacaccacctctttgagttatcgccaccaggtcgccgaagagcttggcaagctgggctctattcaggataaatcccgcggatggtgggttcactccgttctcttgctcgaggccaccacattccgcaccgtaggattactg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61211_sequence/1
type: Sequence
persistent identity: BBa J61211 sequence
display id: BBa_J61211_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgtctcttatacacatcttactagaggatccgttccggaatgtttgatgttatggagcagcaacgatgttacgcagcagggcagtcgccctaaaacaaagttaggtggctcaagtatgggcatcattcgcacatgtaggctcggccctgaccaagtcaaatccatgcgggctgctcttgatcttttcggtcgtgagttcggagacgtagccacctactcccaacatcagccggactccgattacctcgggaacttgctccgtagtaagacattcatcgcgcttgctgccttcgaccaagaagcggttgttggcgctctcgcggcttacgttctgcccaagtttgagcagccgcgtagtgagatctatatctatgatctcgcagtctccggagagcaccggaggcagggcattgccaccgcgctcatcaatctcctcaagcatgaggccaacgcgcttggtgcttatgtgatctacgtgcaagcagattacggtgacgatcccgcagtggctctctatacaaagttgggcatacgggaagaagtgatgcactttgatatcgacccaagtaccgccacctaacgaatttactagaggaagttcctatactttttagagaataggaacttctactagagtgattcgcacgggcccatggctaattcccatgtcagccgttaagtgttcctgtgtcactcaaaattgctttgagaggctctaagggcttctcagtgcgttacatccctggcttgttgtccacaaccgttaaaccttaaaagctttaaaagccttatatattcttttttttcttataaaacttaaaaccttagaggctatttaagttgctgatttatattaattttattgttcaaacatgagagcttagtacgtgaaacatgagagcttagtacgttagccatgagagcttagtacgttagccatgagggtttagttcgttaaacatgagagcttagtacgttaaacatgagagcttagtacgtgaaacatgagagcttagtacgtactatcaacaggttgaactgctactagaggaataggaacttcatttaaatggcgcgccttacgccccgccctgccactcatcgcagtactgttgtattcattaagcatctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgtaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgacaactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgccgatcaacgtctcattttcgccaaaagttggcccagggcttcccggtatcaacagggacaccaggatttatttattctgcgaagtgatcttccgtcacaggtaggcgcgctactagaggaagttcctatactttttagagaataggaacttctactagagtaata…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23012_sequence/1
type: Sequence
persistent identity: BBa J23012 sequence
display id: BBa_J23012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccaagcgagctcgatatccgtcggcttgaacgaattgttagacattatttgccgactaccttggtgatctcgcctttcacgtagtggacaaattcttccaactgatctgcgcgcgaggccaagcgatcttcttcttgtccaagataagcctgtctagcttcaagtatgacgggctgatactgggccggcaggcgctccattgcccagtcggcagcgacatccttcggcgcgattttgccggttactgcgctgtaccaaatgcgggacaacgtaagcactacatttcgctcatcgccagcccagtcgggcggcgagttccatagcgttaaggtttcatttagcgcctcaaatagatcctgttcaggaaccggatcaaagagttcctccgccgctggacctaccaaggcaacgctatgttctcttgcttttgtcagcaagatagccagatcaatgtcgtacgtggctggctcgaagatacctgcaagaatgtcattgcgctgccattctccaaattgcagttcgcgcttagctggataacgccacggaatgatgtcgtcgtgcacaacaatggtgacttctacagcgcggagaatctcgctctctccaggggaagccgaagtttccaaaaggtcgttgatcaaagctcgccgcgttgtttcatcaagccttacggtcaccgtaaccagcaaatcaatatcactgtgtggcttcaggccgccatccactgcggagccgtacaaatgtacggccagcaacgtcggttcgagatggcgctcgatgacgccaactacctctgatagttgagtcgatacttcggcgatcaccgcttccctcatgatgtttaactttgttttagggcgactgccctgctgcgtaacatcgttgct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61023_sequence/1
type: Sequence
persistent identity: BBa J61023 sequence
display id: BBa_J61023_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgattctccgccagcatggcttcggccagtgcgtcgagcagcgcccgcttgttcctgaagtgccagtaaagcgccggctgctgaacccccaaccgttccgccagtttgcgtgtcgtcagaccgtctacgccgacctcgttcaacaggtctagggcggcacggatcactgtattcggctgcaactttgtcatgcttgacactttatcactgataaacataatatgtccaccaacttatcagtgataaagaatccgcgcgttcaatcggaccagcggaggctggtccggaggccacatatgataacttctgctcttcatcgtgcggccgactgggctaaatctgtgttctcttcggcggcgctgggtgatcctcgccgtactgcccgcttggttaacgtcgccgcccaattggcaaaatattctggtaaatcaataaccatctcatcagagggtagtaaagccgcccaggaaggcgcttaccgatttatccgcaatcccaacgtttctgccgaggcgatcagaaaggctggcgccatgcaaacagtcaagttggctcaggagtttcccgaactgctggccattgaggacaccacctctttgagttatcgccaccaggtcgccgaagagcttggcaagctgggctctattcaggataaatcccgcggatggtgggttcactccgttctcttgctcgaggccaccacattccgcaccgtaggattactgcatcaggagtggtggatgcgcccggatgaccctgccgatgcggatgaaaaggagagtggcaaatggctggcagcggccgcaactagccggttacgcatgggcagcatgatgagcaacgtgattgcggtctgtgaccgcgaagccgatattcatgcttatctgcaggacaaactggcgcataacgagcgcttcgtggtgcgctccaagcacccacgcaaggacgtagagtctgggttgtatctgtacgaccatctgaagaaccaaccggagttgggtggctatcagatcagcattccgcaaaagggcgtggtggataaacgcggtaaacgtaaaaatcgaccagcccgcaaggcgagcttgagcctgcgcagtgggcgcatcacgctaaaacaggggaatatcacgctcaacgcggtgctggccgaggagattaacccgcccaagggtgagaccccgttgaaatggttgttgctgaccagcgaaccggtcgagtcgctagcccaagccttgcgcgtcatcgacatttatacccatcgctggcggatcgaggagttccataaggcatggaaaaccggagcaggagccgagaggcaacgcatggaggagccggataatctggagcggatggtctcgatcctctcgtttgttgcggtcaggctgttacagctcagagaaagcttcacgccgccgcaagcactcagggcgcaagggctgctaaaggaagcggaacacgtagaaagccagtccgcagaaacggtgctgaccccggatgaatgtcagctactgggctatctggacaagggaaaacgcaagcgcaaagagaaagcaggtagcttgcagtgggcttacatggcgatagctagactgggcggttttatggacagcaagcgaaccggaattgccagctggggcgccctctgggaaggttgggaagccctgcaaagtaaactggatggctttcttgccgccaaggatctgatggcgcaggggatcaagatctgatccgtc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61202_sequence/1
type: Sequence
persistent identity: BBa J61202 sequence
display id: BBa_J61202_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgtctcttatacacatcttactagaggatccgttccggaatgtttgatgttatggagcagcaacgatgttacgcagcagggcagtcgccctaaaacaaagttaggtggctcaagtatgggcatcattcgcacatgtaggctcggccctgaccaagtcaaatccatgcgggctgctcttgatcttttcggtcgtgagttcggagacgtagccacctactcccaacatcagccggactccgattacctcgggaacttgctccgtagtaagacattcatcgcgcttgctgccttcgaccaagaagcggttgttggcgctctcgcggcttacgttctgcccaagtttgagcagccgcgtagtgagatctatatctatgatctcgcagtctccggagagcaccggaggcagggcattgccaccgcgctcatcaatctcctcaagcatgaggccaacgcgcttggtgcttatgtgatctacgtgcaagcagattacggtgacgatcccgcagtggctctctatacaaagttgggcatacgggaagaagtgatgcactttgatatcgacccaagtaccgccacctaacgaatt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61210_sequence/1
type: Sequence
persistent identity: BBa J61210 sequence
display id: BBa_J61210_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaagttcctatactttttagagaataggaacttctactagagtgattcgcacgggcccatggctaattcccatgtcagccgttaagtgttcctgtgtcactcaaaattgctttgagaggctctaagggcttctcagtgcgttacatccctggcttgttgtccacaaccgttaaaccttaaaagctttaaaagccttatatattcttttttttcttataaaacttaaaaccttagaggctatttaagttgctgatttatattaattttattgttcaaacatgagagcttagtacgtgaaacatgagagcttagtacgttagccatgagagcttagtacgttagccatgagggtttagttcgttaaacatgagagcttagtacgttaaacatgagagcttagtacgtgaaacatgagagcttagtacgtactatcaacaggttgaactgctactagaggaataggaacttcatttaaatggcgcgccttacgccccgccctgccactcatcgcagtactgttgtattcattaagcatctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgtaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgacaactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgccgatcaacgtctcattttcgccaaaagttggcccagggcttcccggtatcaacagggacaccaggatttatttattctgcgaagtgatcttccgtcacaggtaggcgcgctactagaggaagttcctatactttttagagaataggaacttctactagagtaatacgactcactatagggagatactagagagatgtgtataagagacag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J01003_sequence/1
type: Sequence
persistent identity: BBa J01003 sequence
display id: BBa_J01003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gccgccttttcctcaatcgctcttcgttcgtctggaaggcagtacaccttgataggtgggctgcccttcctggttggcttggtttcatcagccatccgcttgccctcatctgttacgccggcggtagccggccagcctcgcagagcaggattcccgttgagcaccgccaggtgcgaataagggacagtgaagaaggaacacccgctcgcgggtgggcctacttcacctatcctgcccggctgacgccgttggatacaccaaggaaagtctacacgaaccctttggcaaaatcctgtatatcgtgcgaaaaaggatggatataccgaaaaaatcgctataatgaccccgaagcagggttatgcagcggaaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61021_sequence/1
type: Sequence
persistent identity: BBa J61021 sequence
display id: BBa_J61021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgtctcttatacacatct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61022_sequence/1
type: Sequence
persistent identity: BBa J61022 sequence
display id: BBa_J61022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agatgtgtataagagacag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61201_sequence/1
type: Sequence
persistent identity: BBa J61201 sequence
display id: BBa_J61201_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gatccgttccggaatgtttgatgttatggagcagcaacgatgttacgcagcagggcagtcgccctaaaacaaagttaggtggctcaagtatgggcatcattcgcacatgtaggctcggccctgaccaagtcaaatccatgcgggctgctcttgatcttttcggtcgtgagttcggagacgtagccacctactcccaacatcagccggactccgattacctcgggaacttgctccgtagtaagacattcatcgcgcttgctgccttcgaccaagaagcggttgttggcgctctcgcggcttacgttctgcccaagtttgagcagccgcgtagtgagatctatatctatgatctcgcagtctccggagagcaccggaggcagggcattgccaccgcgctcatcaatctcctcaagcatgaggccaacgcgcttggtgcttatgtgatctacgtgcaagcagattacggtgacgatcccgcagtggctctctatacaaagttgggcatacgggaagaagtgatgcactttgatatcgacccaagtaccgccacctaacgaatt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0085_sequence/1
type: Sequence
persistent identity: BBa R0085 sequence
display id: BBa_R0085_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61000_sequence/1
type: Sequence
persistent identity: BBa J61000 sequence
display id: BBa_J61000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaataggaacttcatttaaatggcgcgccttacgccccgccctgccactcatcgcagtactgttgtattcattaagcatctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgtaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgacaactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgccgatcaacgtctcattttcgccaaaagttggcccagggcttcccggtatcaacagggacaccaggatttatttattctgcgaagtgatcttccgtcacaggtaggcgcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61001_sequence/1
type: Sequence
persistent identity: BBa J61001 sequence
display id: BBa_J61001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgattcgcacgggcccatggctaattcccatgtcagccgttaagtgttcctgtgtcactcaaaattgctttgagaggctctaagggcttctcagtgcgttacatccctggcttgttgtccacaaccgttaaaccttaaaagctttaaaagccttatatattcttttttttcttataaaacttaaaaccttagaggctatttaagttgctgatttatattaattttattgttcaaacatgagagcttagtacgtgaaacatgagagcttagtacgttagccatgagagcttagtacgttagccatgagggtttagttcgttaaacatgagagcttagtacgttaaacatgagagcttagtacgtgaaacatgagagcttagtacgtactatcaacaggttgaactgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61020_sequence/1
type: Sequence
persistent identity: BBa J61020 sequence
display id: BBa_J61020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaagttcctatactttttagagaataggaacttc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z