LOCUS       BBa_J63010              3266 bp    DNA     linear       06-May-2024
DEFINITION  Protein fusion vector (Silver lab standard)
ACCESSION   BBa_J63010
VERSION     BBa_J63010.1  
FEATURES             Location/Qualifiers
     CDS             1253..2113
                     /label=beta-lactamase (bla gene)
     misc_feature    1..20
                     /label=modified BioBrick suffix
     misc_feature    479..1093
                     /label=rep pMB1 replication origin
     misc_feature    2632..2938
                     /label=f1 origin
     CDS             3028..3187
                     /label=lacZ alpha peptide
     misc_feature    494..1046
                     /label=RNAII transcript
     misc_feature    936..1043
                     /label=RNAI transcript
     misc_feature    3246..3266
                     /label=modified BioBrick prefix
     misc_feature    3260..3260
                     /label=incorrect silver fusion prefix
ORIGIN
        1 actagtagcg gccgctgcag agcaagagac gactgactga ctgactggaa agaggaaggg
       61 ctggaagagg aaggagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
      121 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
      181 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
      241 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
      301 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
      361 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
      421 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      481 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      541 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      601 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      661 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
      721 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
      781 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
      841 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
      901 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
      961 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     1021 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     1081 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     1141 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     1201 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     1261 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     1321 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     1381 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     1441 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     1501 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     1561 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     1621 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     1681 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     1741 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     1801 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     1861 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     1921 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     1981 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     2041 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     2101 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     2161 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     2221 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     2281 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     2341 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     2401 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     2461 atgcggcatc agagcagatt gtactgagag tgcaccataa aattgtaaac gttaatattt
     2521 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa tagaccgaaa
     2581 tcggcaaaat cccttataaa tcaaaagaat agcccgagat agagttgagt gttgttccag
     2641 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     2701 tctatcaggg cgatggccca ctacgtgaac catcacccaa atcaagtttt ttggggtcga
     2761 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     2821 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaagg
     2881 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     2941 cgctacaggg cgcgtactat ggttgctttg acgtatgcgg tgtgaaatac cgcacagatg
     3001 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
     3061 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     3121 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     3181 cagtgaattg gaggctacag tcagtggaga ggactttcac tgactgactg actgcgtctc
     3241 aacctgaatt cgcggccgca tctaga
//