BBa_J64004
1
BBa_J64004
DH domain constitutively active
2008-01-15T12:00:00Z
2015-08-31T01:56:26Z
Human Genomic DNA library clone with 2 point mutations
This protein coding frame is a Guanine Exchange Factor (GEF) domain from human eukaryotic with point mutations to make it constitutively active. This protein can be secreted at high titer via the Salmonella T3S using the Voigt Lab secretion circuit.
false
false
_98_
0
2407
98
Not in stock
false
This part contains 2 PstI sites and is incompatible with biobrick standard assembly
false
Dan Widmaier
annotation1960212
1
HindIII Restriction Site
range1960212
1
1
6
annotation1959594
1
DH Domain
range1959594
1
7
612
annotation1960213
1
NotI Restriction Site
range1960213
1
613
621
BBa_J64004_sequence
1
aagcttgatatgttgaccccaactgaaagaaagcgacaaggatacatccacgagctcattgtcaccgaggagaactatgtgaatgacctgcagctggtcacagagatttttcaaaaacccctgatggagtctgagctgctgacagaaaaagaggttgctatgatttttgtgaactggaaggagctgattatgtgtaatatcaaactactaaaagcgctgagagtccgcaagaagatgtccggggagaagatgcctgtgaagatgattggagacatcctgagcgcacagctgccgcacatgcagccctacatccgcttctgcagccgccagctcaacggggctgccctgatccagcagaagacggacgaggccccagacttcaaggagttcgtcaaaagattggcaatggatcctcggtgtaaagggatgccactctctagttttatactgaagcctatgcaacgggtaacaagatacccactgatcattaaaaatatcctggaaaacacccctgaaaaccacccggaccacagccacttgaagcacgccctggagaaggcggaagagctctgttcccaggtgaacgaaggggtgcgggagaaggagaactctagcggccgc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z