LOCUS       BBa_J64032              1877 bp    DNA     linear       22-Oct-2024
DEFINITION  pCASP SPI-1 Secretion Circuit
ACCESSION   BBa_J64032
VERSION     BBa_J64032.1  
FEATURES             Location/Qualifiers
     promoter        1..143
                     /label=sicA Promoter
     misc_feature    54..65
                     /label=InvF:SicA Binding Box
     misc_feature    71..77
                     /label=-35 Hexamer
     misc_feature    94..100
                     /label=-10 Hexamer
     misc_feature    106..106
                     /label=+1 Transcriptional Start Site
     promoter        1..143
                     /label=BBa_J64001
     misc_feature    144..163
                     /label=misc
     RBS             144..163
                     /label=BBa_J64022
     CDS             164..556
                     /label=sicP
     CDS             543..1043
                     /label=sptP167
     RBS             522..542
                     /label=sptP RBS
     misc_feature    164..1043
                     /label=BBa_J64008
     CDS             1047..1067
                     /label=TEV protease recognition sequence
     CDS             1044..1046
                     /label=Gly
     CDS             1068..1070
                     /label=Gly
     CDS             1044..1070
                     /label=BBa_J64007
     CDS             1077..1682
                     /label=DH Domain
     misc_feature    1071..1076
                     /label=HindIII Restriction Site
     misc_feature    1683..1691
                     /label=NotI Restriction Site
     CDS             1071..1691
                     /label=BBa_J64004
     CDS             1692..1763
                     /label=3x FLAG Tag
     misc_feature    1764..1766
                     /label=STOP Codon
     misc_feature    1767..1772
                     /label=XbaI Restriction Site
     misc_feature    1692..1772
                     /label=BBa_J64005
     misc_feature    1773..1877
                     /label=T1 Terminator
     terminator      1773..1877
                     /label=BBa_J64043
ORIGIN
        1 ccacaagaaa acgaggtacg gcattgagcc gcgtaaggca gtagcgatgt attcattggg
       61 cgttttttga atgttcacta accaccgtcg gggtttaata actgcatcag ataaacgcag
      121 tcgttaagtt ctacaaagtc ggtacagata acaggagtaa gtaatgcaag cacaccagga
      181 tattatcgct aatattggtg agaaattggg tttaccgctc acttttgacg acaacaatca
      241 gtgcttatta ttactcgata gcgatatttt tacgtctatt gaagctaaag atgatatctg
      301 gttattgaac ggtatgatta taccgttatc gcctgtttgt ggcgattcta tctggcggca
      361 gattatggtg attaatggtg aactggctgc gaataatgaa ggtacgttag cgtatattga
      421 tgccgcagag acgttgttgc ttatacatgc aattaccgat ctgacaaata cttaccatat
      481 tatatcgcag cttgagtcat ttgtgaatca gcaggaagcg ctcaaaaaca tactgcagga
      541 atatgctaaa gtatgaggag agaaaattga ataatttaac gttgtcttcg ttttcaaaag
      601 ttggtgtgtc gaatgatgcc cgactttata ttgctaagga aaatactgat aaggcatatg
      661 ttgcgcctga aaaattttcg tcaaaagtat taacctggct tggaaaaatg ccgttattta
      721 aaaacactga agtggtgcaa aaacatacgg aaaatatcag agtacaggac caaaagattt
      781 tacagacatt tctccatgca ctaacggaaa aatatgggga aacagcggtt aatgacgcac
      841 tgttaatgtc ccgtataaat atgaacaaac cccttaccca acgtttagca gtgcagatca
      901 cggagtgtgt aaaagctgct gacgaagggt ttataaacct tattaagagc aaggataatg
      961 ttggtgtcag gaatgccgct ttagtcataa aaggcggcga tacaaaagtg gcagaaaaaa
     1021 ataacgatgt tggagcagaa agtggagaga atttgtattt tcagtcagga aagcttgata
     1081 tgttgacccc aactgaaaga aagcgacaag gatacatcca cgagctcatt gtcaccgagg
     1141 agaactatgt gaatgacctg cagctggtca cagagatttt tcaaaaaccc ctgatggagt
     1201 ctgagctgct gacagaaaaa gaggttgcta tgatttttgt gaactggaag gagctgatta
     1261 tgtgtaatat caaactacta aaagcgctga gagtccgcaa gaagatgtcc ggggagaaga
     1321 tgcctgtgaa gatgattgga gacatcctga gcgcacagct gccgcacatg cagccctaca
     1381 tccgcttctg cagccgccag ctcaacgggg ctgccctgat ccagcagaag acggacgagg
     1441 ccccagactt caaggagttc gtcaaaagat tggcaatgga tcctcggtgt aaagggatgc
     1501 cactctctag ttttatactg aagcctatgc aacgggtaac aagataccca ctgatcatta
     1561 aaaatatcct ggaaaacacc cctgaaaacc acccggacca cagccacttg aagcacgccc
     1621 tggagaaggc ggaagagctc tgttcccagg tgaacgaagg ggtgcgggag aaggagaact
     1681 ctagcggccg cgattataaa gatgacgatg acaaggatta taaagatgac gatgacaagg
     1741 attataaaga tgacgatgac aagtaatcta gaggcatcaa ataaaacgaa aggctcagtc
     1801 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac
     1861 aaatccgccg ccctaga
//