BBa_J64007
1
BBa_J64007
TEVr
2008-01-15T12:00:00Z
2015-08-31T01:56:26Z
Synthetically created from oligonucleotide synthesis
The Tobacco Etch Virus (TEV) protease cleavage recognition sequence. This DNA sequence encodes an amino acid sequence recognized by TEV protease (ENLYFQS) for efficient cleavage. The sequence is flanked by glycines on the ends to increase flexibility and accessibility to the protease. After proteolytic processing the C-terminal fragment retains only an S residue, however by the design of this part it will leave an SG dipeptide which becomes the new N-terminus of the mature polypeptide.
false
false
_98_
0
2407
98
Not in stock
false
This part was codon optimized for expression in E.Coli.
false
Dan Widmaier
annotation1959597
1
TEV protease recognition sequence
range1959597
1
4
24
annotation1959598
1
Gly
range1959598
1
1
3
annotation1959599
1
Gly
range1959599
1
25
27
BBa_J64022
1
BBa_J64022
sicA RBS
2008-01-16T12:00:00Z
2015-08-31T01:56:26Z
Salmonella LT2 Genomic DNA
This is the natural RBS from the sicA chaperone (BBa_J6021) in the Salmonella Type III Secretion System.
false
false
_98_
0
2407
98
Not in stock
false
This part was not designed with standard biobrick assembly in mind.
false
Dan Widmaier
annotation1959621
1
misc
range1959621
1
1
20
BBa_J64001
1
BBa_J64001
psicA from <I>Salmonella</I>
2008-01-14T12:00:00Z
2015-08-31T01:56:26Z
Salmonella LT2 Genomic DNA
The sicA promoter is a regulatory element in the Salmonella SPI-1 secretion system. It controls the expression of secreted effector proteins in the natural system and turns on late. Please reference Temme et al, 2008, JMB for exact dynamics of this promoter and associated feedback mechanisms. This promoter only functions in Salmonella strains containing SPI-1.
false
false
_98_
0
2407
98
Not in stock
false
This part can only be used in salmonella strains.
false
Dan Widmaier
annotation1959627
1
sicA Promoter
range1959627
1
1
143
annotation1959631
1
+1 Transcriptional Start Site
range1959631
1
106
106
annotation1959628
1
InvF:SicA Binding Box
range1959628
1
54
65
annotation1959629
1
-35 Hexamer
range1959629
1
71
77
annotation1959630
1
-10 Hexamer
range1959630
1
94
100
BBa_J64008
1
SicP-SptP
SicP-SptP chaperone-secretion tag pair from Salmonella type III secretion system
2008-01-15T12:00:00Z
2015-08-31T01:56:26Z
Salmonella LT2 Genomic DNA
This part is the natural implementation of the SicP chaperone (BBa_J64002) and SptP secretion signal (BBa_J64003) from the Salmonella Type III Secretion System (T3S, the Salmonella T3S coding region is referred to as Salmonella Pathogeneity Island 1 [SPI-1]). In the natural implementation the coding regions of SicP and SptP overlap out of frame. This part captures that configuration for use in creating secreted protein / chaperone pairs.
false
false
_98_
0
2407
98
Not in stock
false
This part is not compatible with biobrick standard assembly.
false
Dan Widmaier
annotation1959600
1
sicP
range1959600
1
1
393
annotation1959601
1
sptP167
range1959601
1
380
880
annotation1959602
1
sptP RBS
range1959602
1
359
379
BBa_J64043
1
BBa_J64043
T1 terminator
2008-01-21T12:00:00Z
2015-05-08T01:08:16Z
Commercial plasmid DNA
This is the T1 terminator from the pPROTet 6xHN vector commercially available from Clontech.
false
false
_98_
0
2407
98
Not in stock
false
This sequence is not designed with biobrick standard assembly compatibility in mind.
false
Dan Widmaier
annotation1960020
1
T1 Terminator
range1960020
1
1
105
BBa_J64032
1
BBa_J64032
pCASP SPI-1 Secretion Circuit
2008-01-16T12:00:00Z
2015-08-31T01:56:26Z
This is a composite part consisting of both natural and synthetic DNA components
The pCASP circuit is a protein expression and secretion circuit. The system is currently only functional when expressed in Salmonella under SPI-1 expressing conditions. When SPI-1 is expressed the pCASP circuit will be activated for expression at the same time natural effectors would normally turn on and secrete. This circuit will express a protein of interest fused to the SptP167 secretion signal along with co-expression of the sicP chaperone. This combination is sufficient to direct the protein of interest to the export machinery and elicit secretion to the extracellular environment. In the initial configuration of this circuit the DH guanine exchange factor is used as a de-bugging secreted protein.
Note: Some evidence exists of impassible substrates for the secretion machinery. Most of these proteins are highly thermodynamically stable domains that cannot be unfolded to transit through the secretion system.
false
false
_98_
0
2407
98
Not in stock
false
This device was not designed with biobrick standard assembly in mind and has several of the scar sequence sites throughout the circuit.
false
Dan Widmaier
component1960238
1
BBa_J64005
component1960230
1
BBa_J64007
component1960234
1
BBa_J64004
component1960222
1
BBa_J64022
component1960226
1
BBa_J64008
component1960240
1
BBa_J64043
component1960220
1
BBa_J64001
annotation1960220
1
BBa_J64001
range1960220
1
1
143
annotation1960226
1
BBa_J64008
range1960226
1
164
1043
annotation1960238
1
BBa_J64005
range1960238
1
1692
1772
annotation1960240
1
BBa_J64043
range1960240
1
1773
1877
annotation1960222
1
BBa_J64022
range1960222
1
144
163
annotation1960234
1
BBa_J64004
range1960234
1
1071
1691
annotation1960230
1
BBa_J64007
range1960230
1
1044
1070
BBa_J64005
1
3x FLAG
3x FLAG affinity tag
2008-01-15T12:00:00Z
2015-08-31T01:56:26Z
Synthetic DNA from Oligonucleotide synthesis
A 3 repeat FLAG (DYKDDDDK) antigen epitope tag used for marking proteins of interest. Proteins carrying this tag can be easily detected using commercial western blotting reagents.
false
false
_98_
0
2407
98
Not in stock
false
This sequence was designed as part of an oligonucleotide primer on each strand then annealed and ligated into the vector.
false
Dan Widmaier
annotation1959595
1
3x FLAG Tag
range1959595
1
1
72
annotation1960214
1
XbaI Restriction Site
range1960214
1
76
81
annotation1959596
1
STOP Codon
range1959596
1
73
75
BBa_J64004
1
BBa_J64004
DH domain constitutively active
2008-01-15T12:00:00Z
2015-08-31T01:56:26Z
Human Genomic DNA library clone with 2 point mutations
This protein coding frame is a Guanine Exchange Factor (GEF) domain from human eukaryotic with point mutations to make it constitutively active. This protein can be secreted at high titer via the Salmonella T3S using the Voigt Lab secretion circuit.
false
false
_98_
0
2407
98
Not in stock
false
This part contains 2 PstI sites and is incompatible with biobrick standard assembly
false
Dan Widmaier
annotation1960212
1
HindIII Restriction Site
range1960212
1
1
6
annotation1959594
1
DH Domain
range1959594
1
7
612
annotation1960213
1
NotI Restriction Site
range1960213
1
613
621
BBa_J64001_sequence
1
ccacaagaaaacgaggtacggcattgagccgcgtaaggcagtagcgatgtattcattgggcgttttttgaatgttcactaaccaccgtcggggtttaataactgcatcagataaacgcagtcgttaagttctacaaagtcggt
BBa_J64004_sequence
1
aagcttgatatgttgaccccaactgaaagaaagcgacaaggatacatccacgagctcattgtcaccgaggagaactatgtgaatgacctgcagctggtcacagagatttttcaaaaacccctgatggagtctgagctgctgacagaaaaagaggttgctatgatttttgtgaactggaaggagctgattatgtgtaatatcaaactactaaaagcgctgagagtccgcaagaagatgtccggggagaagatgcctgtgaagatgattggagacatcctgagcgcacagctgccgcacatgcagccctacatccgcttctgcagccgccagctcaacggggctgccctgatccagcagaagacggacgaggccccagacttcaaggagttcgtcaaaagattggcaatggatcctcggtgtaaagggatgccactctctagttttatactgaagcctatgcaacgggtaacaagatacccactgatcattaaaaatatcctggaaaacacccctgaaaaccacccggaccacagccacttgaagcacgccctggagaaggcggaagagctctgttcccaggtgaacgaaggggtgcgggagaaggagaactctagcggccgc
BBa_J64032_sequence
1
ccacaagaaaacgaggtacggcattgagccgcgtaaggcagtagcgatgtattcattgggcgttttttgaatgttcactaaccaccgtcggggtttaataactgcatcagataaacgcagtcgttaagttctacaaagtcggtacagataacaggagtaagtaatgcaagcacaccaggatattatcgctaatattggtgagaaattgggtttaccgctcacttttgacgacaacaatcagtgcttattattactcgatagcgatatttttacgtctattgaagctaaagatgatatctggttattgaacggtatgattataccgttatcgcctgtttgtggcgattctatctggcggcagattatggtgattaatggtgaactggctgcgaataatgaaggtacgttagcgtatattgatgccgcagagacgttgttgcttatacatgcaattaccgatctgacaaatacttaccatattatatcgcagcttgagtcatttgtgaatcagcaggaagcgctcaaaaacatactgcaggaatatgctaaagtatgaggagagaaaattgaataatttaacgttgtcttcgttttcaaaagttggtgtgtcgaatgatgcccgactttatattgctaaggaaaatactgataaggcatatgttgcgcctgaaaaattttcgtcaaaagtattaacctggcttggaaaaatgccgttatttaaaaacactgaagtggtgcaaaaacatacggaaaatatcagagtacaggaccaaaagattttacagacatttctccatgcactaacggaaaaatatggggaaacagcggttaatgacgcactgttaatgtcccgtataaatatgaacaaaccccttacccaacgtttagcagtgcagatcacggagtgtgtaaaagctgctgacgaagggtttataaaccttattaagagcaaggataatgttggtgtcaggaatgccgctttagtcataaaaggcggcgatacaaaagtggcagaaaaaaataacgatgttggagcagaaagtggagagaatttgtattttcagtcaggaaagcttgatatgttgaccccaactgaaagaaagcgacaaggatacatccacgagctcattgtcaccgaggagaactatgtgaatgacctgcagctggtcacagagatttttcaaaaacccctgatggagtctgagctgctgacagaaaaagaggttgctatgatttttgtgaactggaaggagctgattatgtgtaatatcaaactactaaaagcgctgagagtccgcaagaagatgtccggggagaagatgcctgtgaagatgattggagacatcctgagcgcacagctgccgcacatgcagccctacatccgcttctgcagccgccagctcaacggggctgccctgatccagcagaagacggacgaggccccagacttcaaggagttcgtcaaaagattggcaatggatcctcggtgtaaagggatgccactctctagttttatactgaagcctatgcaacgggtaacaagatacccactgatcattaaaaatatcctggaaaacacccctgaaaaccacccggaccacagccacttgaagcacgccctggagaaggcggaagagctctgttcccaggtgaacgaaggggtgcgggagaaggagaactctagcggccgcgattataaagatgacgatgacaaggattataaagatgacgatgacaaggattataaagatgacgatgacaagtaatctagaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgccctaga
BBa_J64043_sequence
1
ggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgccctaga
BBa_J64008_sequence
1
atgcaagcacaccaggatattatcgctaatattggtgagaaattgggtttaccgctcacttttgacgacaacaatcagtgcttattattactcgatagcgatatttttacgtctattgaagctaaagatgatatctggttattgaacggtatgattataccgttatcgcctgtttgtggcgattctatctggcggcagattatggtgattaatggtgaactggctgcgaataatgaaggtacgttagcgtatattgatgccgcagagacgttgttgcttatacatgcaattaccgatctgacaaatacttaccatattatatcgcagcttgagtcatttgtgaatcagcaggaagcgctcaaaaacatactgcaggaatatgctaaagtatgaggagagaaaattgaataatttaacgttgtcttcgttttcaaaagttggtgtgtcgaatgatgcccgactttatattgctaaggaaaatactgataaggcatatgttgcgcctgaaaaattttcgtcaaaagtattaacctggcttggaaaaatgccgttatttaaaaacactgaagtggtgcaaaaacatacggaaaatatcagagtacaggaccaaaagattttacagacatttctccatgcactaacggaaaaatatggggaaacagcggttaatgacgcactgttaatgtcccgtataaatatgaacaaaccccttacccaacgtttagcagtgcagatcacggagtgtgtaaaagctgctgacgaagggtttataaaccttattaagagcaaggataatgttggtgtcaggaatgccgctttagtcataaaaggcggcgatacaaaagtggcagaaaaaaataacgatgttggagcagaaagt
BBa_J64005_sequence
1
gattataaagatgacgatgacaaggattataaagatgacgatgacaaggattataaagatgacgatgacaagtaatctaga
BBa_J64022_sequence
1
acagataacaggagtaagta
BBa_J64007_sequence
1
ggagagaatttgtattttcagtcagga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z