LOCUS       BBa_J64033              1498 bp    DNA     linear       24-Oct-2024
DEFINITION  pCASP - Chaperone
ACCESSION   BBa_J64033
VERSION     BBa_J64033.1  
FEATURES             Location/Qualifiers
     promoter        1..143
                     /label=sicA Promoter
     misc_feature    54..65
                     /label=InvF:SicA Binding Box
     misc_feature    71..77
                     /label=-35 Hexamer
     misc_feature    94..100
                     /label=-10 Hexamer
     misc_feature    106..106
                     /label=+1 Transcriptional Start Site
     promoter        1..143
                     /label=BBa_J64001
     misc_feature    144..163
                     /label=misc
     RBS             144..163
                     /label=BBa_J64022
     CDS             164..664
                     /label=SptP167
     CDS             164..664
                     /label=BBa_J64003
     CDS             668..688
                     /label=TEV protease recognition sequence
     CDS             665..667
                     /label=Gly
     CDS             689..691
                     /label=Gly
     CDS             665..691
                     /label=BBa_J64007
     CDS             698..1303
                     /label=DH Domain
     misc_feature    692..697
                     /label=HindIII Restriction Site
     misc_feature    1304..1312
                     /label=NotI Restriction Site
     CDS             692..1312
                     /label=BBa_J64004
     CDS             1313..1384
                     /label=3x FLAG Tag
     misc_feature    1385..1387
                     /label=STOP Codon
     misc_feature    1388..1393
                     /label=XbaI Restriction Site
     misc_feature    1313..1393
                     /label=BBa_J64005
     misc_feature    1394..1498
                     /label=T1 Terminator
     terminator      1394..1498
                     /label=BBa_J64043
ORIGIN
        1 ccacaagaaa acgaggtacg gcattgagcc gcgtaaggca gtagcgatgt attcattggg
       61 cgttttttga atgttcacta accaccgtcg gggtttaata actgcatcag ataaacgcag
      121 tcgttaagtt ctacaaagtc ggtacagata acaggagtaa gtaatgctaa agtatgagga
      181 gagaaaattg aataatttaa cgttgtcttc gttttcaaaa gttggtgtgt cgaatgatgc
      241 ccgactttat attgctaagg aaaatactga taaggcatat gttgcgcctg aaaaattttc
      301 gtcaaaagta ttaacctggc ttggaaaaat gccgttattt aaaaacactg aagtggtgca
      361 aaaacatacg gaaaatatca gagtacagga ccaaaagatt ttacagacat ttctccatgc
      421 actaacggaa aaatatgggg aaacagcggt taatgacgca ctgttaatgt cccgtataaa
      481 tatgaacaaa ccccttaccc aacgtttagc agtgcagatc acggagtgtg taaaagctgc
      541 tgacgaaggg tttataaacc ttattaagag caaggataat gttggtgtca ggaatgccgc
      601 tttagtcata aaaggcggcg atacaaaagt ggcagaaaaa aataacgatg ttggagcaga
      661 aagtggagag aatttgtatt ttcagtcagg aaagcttgat atgttgaccc caactgaaag
      721 aaagcgacaa ggatacatcc acgagctcat tgtcaccgag gagaactatg tgaatgacct
      781 gcagctggtc acagagattt ttcaaaaacc cctgatggag tctgagctgc tgacagaaaa
      841 agaggttgct atgatttttg tgaactggaa ggagctgatt atgtgtaata tcaaactact
      901 aaaagcgctg agagtccgca agaagatgtc cggggagaag atgcctgtga agatgattgg
      961 agacatcctg agcgcacagc tgccgcacat gcagccctac atccgcttct gcagccgcca
     1021 gctcaacggg gctgccctga tccagcagaa gacggacgag gccccagact tcaaggagtt
     1081 cgtcaaaaga ttggcaatgg atcctcggtg taaagggatg ccactctcta gttttatact
     1141 gaagcctatg caacgggtaa caagataccc actgatcatt aaaaatatcc tggaaaacac
     1201 ccctgaaaac cacccggacc acagccactt gaagcacgcc ctggagaagg cggaagagct
     1261 ctgttcccag gtgaacgaag gggtgcggga gaaggagaac tctagcggcc gcgattataa
     1321 agatgacgat gacaaggatt ataaagatga cgatgacaag gattataaag atgacgatga
     1381 caagtaatct agaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     1441 tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gccctaga
//