BBa_J64033
type: ComponentDefinition
persistent identity: BBa J64033
display id: BBa_J64033
version: 1
was generated by: iGEM to SBOL conversion
description: pCASP - Chaperone
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
mutable provenance: This is a composite part with both synthetic and genomic source DNA
owned by: james
owned by: myers
mutable description: This is a variant of the pCASP secretion circuit lacking the chaperone (sicP) for the SptP167 secretion tag.
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part was not designed with biobrick standard assembly in mind and might contain scar sequence cutsites throughout.
star: false
creator: Dan Widmaier
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_J64043
component: BBa_J64007
component: BBa_J64003
component: BBa_J64005
component: BBa_J64004
component: BBa_J64022
component: BBa_J64001
sequence annotation: BBa_J64001
sequence annotation: BBa_J64022
sequence annotation: BBa_J64003
sequence annotation: BBa_J64007
sequence annotation: BBa_J64004
sequence annotation: BBa_J64005
sequence annotation: BBa_J64043
sequence: https://synbiohub.org/public/igem/BBa_J64033_sequence/1

BBa_J64043
type: Component
persistent identity: component2225027
display id: component2225027
version: 1
top level: BBa_J64033
definition: BBa_J64043
access: public

BBa_J64007
type: Component
persistent identity: component2225017
display id: component2225017
version: 1
top level: BBa_J64033
definition: BBa_J64007
access: public

BBa_J64003
type: Component
persistent identity: component2225013
display id: component2225013
version: 1
top level: BBa_J64033
definition: BBa_J64003
access: public

BBa_J64005
type: Component
persistent identity: component2225025
display id: component2225025
version: 1
top level: BBa_J64033
definition: 3x FLAG
access: public

BBa_J64004
type: Component
persistent identity: component2225021
display id: component2225021
version: 1
top level: BBa_J64033
definition: BBa_J64004
access: public

BBa_J64022
type: Component
persistent identity: component2225011
display id: component2225011
version: 1
top level: BBa_J64033
definition: BBa_J64022
access: public

BBa_J64001
type: Component
persistent identity: component2225009
display id: component2225009
version: 1
top level: BBa_J64033
definition: BBa_J64001
access: public

BBa_J64001
type: SequenceAnnotation
persistent identity: annotation2225009
display id: annotation2225009
version: 1
top level: BBa_J64033
location: https://synbiohub.org/public/igem/BBa_J64033/annotation2225009/range2225009/1
component: BBa_J64001

https://synbiohub.org/public/igem/BBa_J64033/annotation2225009/range2225009/1
type: Range
persistent identity: range2225009
display id: range2225009
version: 1
top level: BBa_J64033
direction: direction/reverse
start: 1
end: 143
orientation: inline

BBa_J64022
type: SequenceAnnotation
persistent identity: annotation2225011
display id: annotation2225011
version: 1
top level: BBa_J64033
location: https://synbiohub.org/public/igem/BBa_J64033/annotation2225011/range2225011/1
component: BBa_J64022

https://synbiohub.org/public/igem/BBa_J64033/annotation2225011/range2225011/1
type: Range
persistent identity: range2225011
display id: range2225011
version: 1
top level: BBa_J64033
direction: direction/reverse
start: 144
end: 163
orientation: inline

BBa_J64003
type: SequenceAnnotation
persistent identity: annotation2225013
display id: annotation2225013
version: 1
top level: BBa_J64033
location: https://synbiohub.org/public/igem/BBa_J64033/annotation2225013/range2225013/1
component: BBa_J64003

https://synbiohub.org/public/igem/BBa_J64033/annotation2225013/range2225013/1
type: Range
persistent identity: range2225013
display id: range2225013
version: 1
top level: BBa_J64033
direction: direction/reverse
start: 164
end: 664
orientation: inline

BBa_J64007
type: SequenceAnnotation
persistent identity: annotation2225017
display id: annotation2225017
version: 1
top level: BBa_J64033
location: https://synbiohub.org/public/igem/BBa_J64033/annotation2225017/range2225017/1
component: BBa_J64007

https://synbiohub.org/public/igem/BBa_J64033/annotation2225017/range2225017/1
type: Range
persistent identity: range2225017
display id: range2225017
version: 1
top level: BBa_J64033
direction: direction/reverse
start: 665
end: 691
orientation: inline

BBa_J64004
type: SequenceAnnotation
persistent identity: annotation2225021
display id: annotation2225021
version: 1
top level: BBa_J64033
location: https://synbiohub.org/public/igem/BBa_J64033/annotation2225021/range2225021/1
component: BBa_J64004

https://synbiohub.org/public/igem/BBa_J64033/annotation2225021/range2225021/1
type: Range
persistent identity: range2225021
display id: range2225021
version: 1
top level: BBa_J64033
direction: direction/reverse
start: 692
end: 1312
orientation: inline

BBa_J64005
type: SequenceAnnotation
persistent identity: annotation2225025
display id: annotation2225025
version: 1
top level: BBa_J64033
location: https://synbiohub.org/public/igem/BBa_J64033/annotation2225025/range2225025/1
component: BBa_J64005

https://synbiohub.org/public/igem/BBa_J64033/annotation2225025/range2225025/1
type: Range
persistent identity: range2225025
display id: range2225025
version: 1
top level: BBa_J64033
direction: direction/reverse
start: 1313
end: 1393
orientation: inline

BBa_J64043
type: SequenceAnnotation
persistent identity: annotation2225027
display id: annotation2225027
version: 1
top level: BBa_J64033
location: https://synbiohub.org/public/igem/BBa_J64033/annotation2225027/range2225027/1
component: BBa_J64043

https://synbiohub.org/public/igem/BBa_J64033/annotation2225027/range2225027/1
type: Range
persistent identity: range2225027
display id: range2225027
version: 1
top level: BBa_J64033
direction: direction/reverse
start: 1394
end: 1498
orientation: inline

BBa_J64001
type: ComponentDefinition
persistent identity: BBa J64001
display id: BBa_J64001
version: 1
was generated by: iGEM to SBOL conversion
description: psicA from Salmonella
created: 2008-01-14T12:00:00Z
modified: 2015-08-31T01:56:26Z
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
mutable description: The sicA promoter is a regulatory element in the Salmonella SPI-1 secretion system. It controls the expression of secreted effector proteins in the natural system and turns on late. Please reference Temme et al, 2008, JMB for exact dynamics of this promoter and associated feedback mechanisms. This promoter only functions in Salmonella strains containing SPI-1.
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part can only be used in salmonella strains.
star: false
creator: Dan Widmaier
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: sicA Promoter
sequence annotation: InvF:SicA Binding Box
sequence annotation: -35 Hexamer
sequence annotation: -10 Hexamer
sequence annotation: +1 Transcriptional Start Site
sequence: https://synbiohub.org/public/igem/BBa_J64001_sequence/1

sicA Promoter
type: SequenceAnnotation
persistent identity: annotation1959627
display id: annotation1959627
version: 1
top level: BBa_J64001
location: https://synbiohub.org/public/igem/BBa_J64001/annotation1959627/range1959627/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_J64001/annotation1959627/range1959627/1
type: Range
persistent identity: range1959627
display id: range1959627
version: 1
top level: BBa_J64001
direction: direction/forward
start: 1
end: 143
orientation: inline

InvF:SicA Binding Box
type: SequenceAnnotation
persistent identity: annotation1959628
display id: annotation1959628
version: 1
top level: BBa_J64001
location: https://synbiohub.org/public/igem/BBa_J64001/annotation1959628/range1959628/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_J64001/annotation1959628/range1959628/1
type: Range
persistent identity: range1959628
display id: range1959628
version: 1
top level: BBa_J64001
direction: direction/forward
start: 54
end: 65
orientation: inline

-35 Hexamer
type: SequenceAnnotation
persistent identity: annotation1959629
display id: annotation1959629
version: 1
top level: BBa_J64001
location: https://synbiohub.org/public/igem/BBa_J64001/annotation1959629/range1959629/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J64001/annotation1959629/range1959629/1
type: Range
persistent identity: range1959629
display id: range1959629
version: 1
top level: BBa_J64001
direction: direction/forward
start: 71
end: 77
orientation: inline

-10 Hexamer
type: SequenceAnnotation
persistent identity: annotation1959630
display id: annotation1959630
version: 1
top level: BBa_J64001
location: https://synbiohub.org/public/igem/BBa_J64001/annotation1959630/range1959630/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J64001/annotation1959630/range1959630/1
type: Range
persistent identity: range1959630
display id: range1959630
version: 1
top level: BBa_J64001
direction: direction/forward
start: 94
end: 100
orientation: inline

+1 Transcriptional Start Site
type: SequenceAnnotation
persistent identity: annotation1959631
display id: annotation1959631
version: 1
top level: BBa_J64001
location: https://synbiohub.org/public/igem/BBa_J64001/annotation1959631/range1959631/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_J64001/annotation1959631/range1959631/1
type: Range
persistent identity: range1959631
display id: range1959631
version: 1
top level: BBa_J64001
direction: direction/forward
start: 106
end: 106
orientation: inline

BBa_J64003
type: ComponentDefinition
persistent identity: BBa J64003
display id: BBa_J64003
version: 1
was generated by: iGEM to SBOL conversion
description: sptP167
created: 2008-01-14T12:00:00Z
modified: 2015-08-31T01:56:26Z
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
mutable description: This coding region is the first 167 N-terminal residues from the SptP effector protein in the Salmonella SPI-1 pathogenesis system. This tag is sufficient when used with sicP (BBa_J64002) to direct a fused heterologous protein for secretion through the SPI-1 system. In the natural system this coding frame overlaps with the 3' end of the sicP coding frame.
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This coding sequence was not designed to conform with biobrick standard assembly.
star: false
creator: Dan Widmaier
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: SptP167
sequence: https://synbiohub.org/public/igem/BBa_J64003_sequence/1

SptP167
type: SequenceAnnotation
persistent identity: annotation1959593
display id: annotation1959593
version: 1
top level: BBa_J64003
location: https://synbiohub.org/public/igem/BBa_J64003/annotation1959593/range1959593/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_J64003/annotation1959593/range1959593/1
type: Range
persistent identity: range1959593
display id: range1959593
version: 1
top level: BBa_J64003
direction: direction/forward
start: 1
end: 501
orientation: inline

BBa_J64004
type: ComponentDefinition
persistent identity: BBa J64004
display id: BBa_J64004
version: 1
was generated by: iGEM to SBOL conversion
description: DH domain constitutively active
created: 2008-01-15T12:00:00Z
modified: 2015-08-31T01:56:26Z
mutable provenance: Human Genomic DNA library clone with 2 point mutations
owned by: james
owned by: myers
mutable description: This protein coding frame is a Guanine Exchange Factor (GEF) domain from human eukaryotic with point mutations to make it constitutively active. This protein can be secreted at high titer via the Salmonella T3S using the Voigt Lab secretion circuit.
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part contains 2 PstI sites and is incompatible with biobrick standard assembly
star: false
creator: Dan Widmaier
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: HindIII Restriction Site
sequence annotation: DH Domain
sequence annotation: NotI Restriction Site
sequence: https://synbiohub.org/public/igem/BBa_J64004_sequence/1

HindIII Restriction Site
type: SequenceAnnotation
persistent identity: annotation1960212
display id: annotation1960212
version: 1
top level: BBa_J64004
location: https://synbiohub.org/public/igem/BBa_J64004/annotation1960212/range1960212/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J64004/annotation1960212/range1960212/1
type: Range
persistent identity: range1960212
display id: range1960212
version: 1
top level: BBa_J64004
direction: direction/forward
start: 1
end: 6
orientation: inline

DH Domain
type: SequenceAnnotation
persistent identity: annotation1959594
display id: annotation1959594
version: 1
top level: BBa_J64004
location: https://synbiohub.org/public/igem/BBa_J64004/annotation1959594/range1959594/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J64004/annotation1959594/range1959594/1
type: Range
persistent identity: range1959594
display id: range1959594
version: 1
top level: BBa_J64004
direction: direction/forward
start: 7
end: 612
orientation: inline

NotI Restriction Site
type: SequenceAnnotation
persistent identity: annotation1960213
display id: annotation1960213
version: 1
top level: BBa_J64004
location: https://synbiohub.org/public/igem/BBa_J64004/annotation1960213/range1960213/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J64004/annotation1960213/range1960213/1
type: Range
persistent identity: range1960213
display id: range1960213
version: 1
top level: BBa_J64004
direction: direction/forward
start: 613
end: 621
orientation: inline

3x FLAG
type: ComponentDefinition
persistent identity: BBa J64005
display id: BBa_J64005
version: 1
was generated by: iGEM to SBOL conversion
description: 3x FLAG affinity tag
created: 2008-01-15T12:00:00Z
modified: 2015-08-31T01:56:26Z
mutable provenance: Synthetic DNA from Oligonucleotide synthesis
owned by: james
owned by: myers
mutable description: A 3 repeat FLAG (DYKDDDDK) antigen epitope tag used for marking proteins of interest. Proteins carrying this tag can be easily detected using commercial western blotting reagents.
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This sequence was designed as part of an oligonucleotide primer on each strand then annealed and ligated into the vector.
star: false
creator: Dan Widmaier
type: DnaRegion
role: partType/Tag
role: SO:0000324
sequence annotation: 3x FLAG Tag
sequence annotation: STOP Codon
sequence annotation: XbaI Restriction Site
sequence: https://synbiohub.org/public/igem/BBa_J64005_sequence/1

3x FLAG Tag
type: SequenceAnnotation
persistent identity: annotation1959595
display id: annotation1959595
version: 1
top level: 3x FLAG
location: https://synbiohub.org/public/igem/BBa_J64005/annotation1959595/range1959595/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J64005/annotation1959595/range1959595/1
type: Range
persistent identity: range1959595
display id: range1959595
version: 1
top level: 3x FLAG
direction: direction/forward
start: 1
end: 72
orientation: inline

STOP Codon
type: SequenceAnnotation
persistent identity: annotation1959596
display id: annotation1959596
version: 1
top level: 3x FLAG
location: https://synbiohub.org/public/igem/BBa_J64005/annotation1959596/range1959596/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_J64005/annotation1959596/range1959596/1
type: Range
persistent identity: range1959596
display id: range1959596
version: 1
top level: 3x FLAG
direction: direction/forward
start: 73
end: 75
orientation: inline

XbaI Restriction Site
type: SequenceAnnotation
persistent identity: annotation1960214
display id: annotation1960214
version: 1
top level: 3x FLAG
location: https://synbiohub.org/public/igem/BBa_J64005/annotation1960214/range1960214/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J64005/annotation1960214/range1960214/1
type: Range
persistent identity: range1960214
display id: range1960214
version: 1
top level: 3x FLAG
direction: direction/forward
start: 76
end: 81
orientation: inline

BBa_J64007
type: ComponentDefinition
persistent identity: BBa J64007
display id: BBa_J64007
version: 1
was generated by: iGEM to SBOL conversion
description: TEVr
created: 2008-01-15T12:00:00Z
modified: 2015-08-31T01:56:26Z
mutable provenance: Synthetically created from oligonucleotide synthesis
owned by: james
owned by: myers
mutable description: The Tobacco Etch Virus (TEV) protease cleavage recognition sequence. This DNA sequence encodes an amino acid sequence recognized by TEV protease (ENLYFQS) for efficient cleavage. The sequence is flanked by glycines on the ends to increase flexibility and accessibility to the protease. After proteolytic processing the C-terminal fragment retains only an S residue, however by the design of this part it will leave an SG dipeptide which becomes the new N-terminus of the mature polypeptide.
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part was codon optimized for expression in E.Coli.
star: false
creator: Dan Widmaier
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Gly
sequence annotation: TEV protease recognition sequence
sequence: https://synbiohub.org/public/igem/BBa_J64007_sequence/1

Gly
type: SequenceAnnotation
persistent identity: annotation1959598
display id: annotation1959598
version: 1
top level: BBa_J64007
location: https://synbiohub.org/public/igem/BBa_J64007/annotation1959598/range1959598/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_J64007/annotation1959598/range1959598/1
type: Range
persistent identity: range1959598
display id: range1959598
version: 1
top level: BBa_J64007
direction: direction/forward
start: 1
end: 3
orientation: inline

TEV protease recognition sequence
type: SequenceAnnotation
persistent identity: annotation1959597
display id: annotation1959597
version: 1
top level: BBa_J64007
location: https://synbiohub.org/public/igem/BBa_J64007/annotation1959597/range1959597/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_J64007/annotation1959597/range1959597/1
type: Range
persistent identity: range1959597
display id: range1959597
version: 1
top level: BBa_J64007
direction: direction/forward
start: 4
end: 24
orientation: inline

Gly
type: SequenceAnnotation
persistent identity: annotation1959599
display id: annotation1959599
version: 1
top level: BBa_J64007
location: https://synbiohub.org/public/igem/BBa_J64007/annotation1959599/range1959599/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_J64007/annotation1959599/range1959599/1
type: Range
persistent identity: range1959599
display id: range1959599
version: 1
top level: BBa_J64007
direction: direction/forward
start: 25
end: 27
orientation: inline

BBa_J64022
type: ComponentDefinition
persistent identity: BBa J64022
display id: BBa_J64022
version: 1
was generated by: iGEM to SBOL conversion
description: sicA RBS
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
mutable description: This is the natural RBS from the sicA chaperone (BBa_J6021) in the Salmonella Type III Secretion System.
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part was not designed with standard biobrick assembly in mind.
star: false
creator: Dan Widmaier
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: misc
sequence: https://synbiohub.org/public/igem/BBa_J64022_sequence/1

misc
type: SequenceAnnotation
persistent identity: annotation1959621
display id: annotation1959621
version: 1
top level: BBa_J64022
location: https://synbiohub.org/public/igem/BBa_J64022/annotation1959621/range1959621/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J64022/annotation1959621/range1959621/1
type: Range
persistent identity: range1959621
display id: range1959621
version: 1
top level: BBa_J64022
direction: direction/forward
start: 1
end: 20
orientation: inline

BBa_J64043
type: ComponentDefinition
persistent identity: BBa J64043
display id: BBa_J64043
version: 1
was generated by: iGEM to SBOL conversion
description: T1 terminator
created: 2008-01-21T12:00:00Z
modified: 2015-05-08T01:08:16Z
mutable provenance: Commercial plasmid DNA
owned by: james
owned by: myers
mutable description: This is the T1 terminator from the pPROTet 6xHN vector commercially available from Clontech.
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This sequence is not designed with biobrick standard assembly compatibility in mind.
star: false
creator: Dan Widmaier
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: T1 Terminator
sequence: https://synbiohub.org/public/igem/BBa_J64043_sequence/1

T1 Terminator
type: SequenceAnnotation
persistent identity: annotation1960020
display id: annotation1960020
version: 1
top level: BBa_J64043
location: https://synbiohub.org/public/igem/BBa_J64043/annotation1960020/range1960020/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J64043/annotation1960020/range1960020/1
type: Range
persistent identity: range1960020
display id: range1960020
version: 1
top level: BBa_J64043
direction: direction/forward
start: 1
end: 105
orientation: inline

https://synbiohub.org/public/igem/BBa_J64033_sequence/1
type: Sequence
persistent identity: BBa J64033 sequence
display id: BBa_J64033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccacaagaaaacgaggtacggcattgagccgcgtaaggcagtagcgatgtattcattgggcgttttttgaatgttcactaaccaccgtcggggtttaataactgcatcagataaacgcagtcgttaagttctacaaagtcggtacagataacaggagtaagtaatgctaaagtatgaggagagaaaattgaataatttaacgttgtcttcgttttcaaaagttggtgtgtcgaatgatgcccgactttatattgctaaggaaaatactgataaggcatatgttgcgcctgaaaaattttcgtcaaaagtattaacctggcttggaaaaatgccgttatttaaaaacactgaagtggtgcaaaaacatacggaaaatatcagagtacaggaccaaaagattttacagacatttctccatgcactaacggaaaaatatggggaaacagcggttaatgacgcactgttaatgtcccgtataaatatgaacaaaccccttacccaacgtttagcagtgcagatcacggagtgtgtaaaagctgctgacgaagggtttataaaccttattaagagcaaggataatgttggtgtcaggaatgccgctttagtcataaaaggcggcgatacaaaagtggcagaaaaaaataacgatgttggagcagaaagtggagagaatttgtattttcagtcaggaaagcttgatatgttgaccccaactgaaagaaagcgacaaggatacatccacgagctcattgtcaccgaggagaactatgtgaatgacctgcagctggtcacagagatttttcaaaaacccctgatggagtctgagctgctgacagaaaaagaggttgctatgatttttgtgaactggaaggagctgattatgtgtaatatcaaactactaaaagcgctgagagtccgcaagaagatgtccggggagaagatgcctgtgaagatgattggagacatcctgagcgcacagctgccgcacatgcagccctacatccgcttctgcagccgccagctcaacggggctgccctgatccagcagaagacggacgaggccccagacttcaaggagttcgtcaaaagattggcaatggatcctcggtgtaaagggatgccactctctagttttatactgaagcctatgcaacgggtaacaagatacccactgatcattaaaaatatcctggaaaacacccctgaaaaccacccggaccacagccacttgaagcacgccctggagaaggcggaagagctctgttcccaggtgaacgaaggggtgcgggagaaggagaactctagcggccgcgattataaagatgacgatgacaaggattataaagatgacgatgacaaggattataaagatgacgatgacaagtaatctagaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgccctaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J64001_sequence/1
type: Sequence
persistent identity: BBa J64001 sequence
display id: BBa_J64001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccacaagaaaacgaggtacggcattgagccgcgtaaggcagtagcgatgtattcattgggcgttttttgaatgttcactaaccaccgtcggggtttaataactgcatcagataaacgcagtcgttaagttctacaaagtcggt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J64003_sequence/1
type: Sequence
persistent identity: BBa J64003 sequence
display id: BBa_J64003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgctaaagtatgaggagagaaaattgaataatttaacgttgtcttcgttttcaaaagttggtgtgtcgaatgatgcccgactttatattgctaaggaaaatactgataaggcatatgttgcgcctgaaaaattttcgtcaaaagtattaacctggcttggaaaaatgccgttatttaaaaacactgaagtggtgcaaaaacatacggaaaatatcagagtacaggaccaaaagattttacagacatttctccatgcactaacggaaaaatatggggaaacagcggttaatgacgcactgttaatgtcccgtataaatatgaacaaaccccttacccaacgtttagcagtgcagatcacggagtgtgtaaaagctgctgacgaagggtttataaaccttattaagagcaaggataatgttggtgtcaggaatgccgctttagtcataaaaggcggcgatacaaaagtggcagaaaaaaataacgatgttggagcagaaagt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J64004_sequence/1
type: Sequence
persistent identity: BBa J64004 sequence
display id: BBa_J64004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aagcttgatatgttgaccccaactgaaagaaagcgacaaggatacatccacgagctcattgtcaccgaggagaactatgtgaatgacctgcagctggtcacagagatttttcaaaaacccctgatggagtctgagctgctgacagaaaaagaggttgctatgatttttgtgaactggaaggagctgattatgtgtaatatcaaactactaaaagcgctgagagtccgcaagaagatgtccggggagaagatgcctgtgaagatgattggagacatcctgagcgcacagctgccgcacatgcagccctacatccgcttctgcagccgccagctcaacggggctgccctgatccagcagaagacggacgaggccccagacttcaaggagttcgtcaaaagattggcaatggatcctcggtgtaaagggatgccactctctagttttatactgaagcctatgcaacgggtaacaagatacccactgatcattaaaaatatcctggaaaacacccctgaaaaccacccggaccacagccacttgaagcacgccctggagaaggcggaagagctctgttcccaggtgaacgaaggggtgcgggagaaggagaactctagcggccgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J64005_sequence/1
type: Sequence
persistent identity: BBa J64005 sequence
display id: BBa_J64005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gattataaagatgacgatgacaaggattataaagatgacgatgacaaggattataaagatgacgatgacaagtaatctaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J64007_sequence/1
type: Sequence
persistent identity: BBa J64007 sequence
display id: BBa_J64007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggagagaatttgtattttcagtcagga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J64022_sequence/1
type: Sequence
persistent identity: BBa J64022 sequence
display id: BBa_J64022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acagataacaggagtaagta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J64043_sequence/1
type: Sequence
persistent identity: BBa J64043 sequence
display id: BBa_J64043_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgccctaga
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z