This is a composite part with both synthetic and genomic source DNA
This is a variant of the pCASP secretion circuit lacking the chaperone (sicP) for the SptP167 secretion tag.
This part was not designed with biobrick standard assembly in mind and might contain scar sequence cutsites throughout.
The sicA promoter is a regulatory element in the Salmonella SPI-1 secretion system. It controls the expression of secreted effector proteins in the natural system and turns on late. Please reference Temme et al, 2008, JMB for exact dynamics of this promoter and associated feedback mechanisms. This promoter only functions in Salmonella strains containing SPI-1.
This part can only be used in salmonella strains.
This coding region is the first 167 N-terminal residues from the SptP effector protein in the Salmonella SPI-1 pathogenesis system. This tag is sufficient when used with sicP (BBa_J64002) to direct a fused heterologous protein for secretion through the SPI-1 system. In the natural system this coding frame overlaps with the 3' end of the sicP coding frame.
This coding sequence was not designed to conform with biobrick standard assembly.
Human Genomic DNA library clone with 2 point mutations
This protein coding frame is a Guanine Exchange Factor (GEF) domain from human eukaryotic with point mutations to make it constitutively active. This protein can be secreted at high titer via the Salmonella T3S using the Voigt Lab secretion circuit.
This part contains 2 PstI sites and is incompatible with biobrick standard assembly
Synthetic DNA from Oligonucleotide synthesis
A 3 repeat FLAG (DYKDDDDK) antigen epitope tag used for marking proteins of interest. Proteins carrying this tag can be easily detected using commercial western blotting reagents.
This sequence was designed as part of an oligonucleotide primer on each strand then annealed and ligated into the vector.
Synthetically created from oligonucleotide synthesis
The Tobacco Etch Virus (TEV) protease cleavage recognition sequence. This DNA sequence encodes an amino acid sequence recognized by TEV protease (ENLYFQS) for efficient cleavage. The sequence is flanked by glycines on the ends to increase flexibility and accessibility to the protease. After proteolytic processing the C-terminal fragment retains only an S residue, however by the design of this part it will leave an SG dipeptide which becomes the new N-terminus of the mature polypeptide.
This part was codon optimized for expression in E.Coli.
This is the natural RBS from the sicA chaperone (BBa_J6021) in the Salmonella Type III Secretion System.
This part was not designed with standard biobrick assembly in mind.
This is the T1 terminator from the pPROTet 6xHN vector commercially available from Clontech.
This sequence is not designed with biobrick standard assembly compatibility in mind.
Conversion of the iGEM parts registry to SBOL2.1