BBa_J64038
1
BBa_J64038
invB Chaperone sopE2 Tag
2008-01-16T12:00:00Z
2015-05-08T01:08:16Z
The source parts for this device come from Salmonella LT2 Genomic DNA
This is a variant of the pCASP secretion circuit with the invB chaperone and sopE2 secretion signal
false
false
_98_
0
2407
98
Not in stock
false
This part was not designed with biobrick standard assembly compatibility in mind, it might contain scar sequence cutsites.
false
Dan Widmaier
component1960347
1
BBa_J64011
component1960351
1
BBa_B0030
component1960354
1
BBa_J64016
component1960349
1
BBa_J64009
annotation1960347
1
BBa_J64011
range1960347
1
1
19
annotation1960349
1
BBa_J64009
range1960349
1
20
427
annotation1960351
1
BBa_B0030
range1960351
1
428
442
annotation1960354
1
BBa_J64016
range1960354
1
443
757
BBa_J64011
1
BBa_J64011
invB RBS
2008-01-15T12:00:00Z
2015-08-31T01:56:26Z
Salmonella LT2 Genomic DNA
The natural RBS that drives invB
false
false
_98_
0
2407
98
Not in stock
false
The expression level of this RBS has not been quantified.
false
Dan Widmaier
annotation1959604
1
invB RBS
range1959604
1
1
19
BBa_J64009
1
BBa_J64009
InvB Secretion Chaperone
2008-01-15T12:00:00Z
2015-08-31T01:56:26Z
Salmonella LT2 Genomic DNA
The InvB protein is a promiscuous chaperone in the Type III Secretion system (T3S) that assists in the secretion of SipA, SopA, and SopE2 effector proteins. This chaperone binds to the secretion signal of selected proteins and maintains the N-terminus in an unfolded state for secretion by the T3S. The chaperone is critical in coordinating the timing and localization to the proper secretion mechanism and is vital in secreting heterologous proteins with this system.
false
false
_98_
0
2407
98
Not in stock
false
This part was not designed to be compatible with biobricks standard assembly
false
Dan Widmaier
annotation1959603
1
invB
range1959603
1
1
408
BBa_J64016
1
BBa_J64016
sopE2 Secretion Signal
2008-01-16T12:00:00Z
2015-08-31T01:56:26Z
Salmonella LT2 Genomic DNA
This part is the 105 amino acid secretion signal for the sopE2 effector protein in Salmonella Type III Secretion. The sequence includes components necessary for directing a fused protein to the export apparatus and secretion to the extracellular space. The cognate chaperone for this sequence is invB (BBa_J64009) and is required to be co-expressed for high titer secretion of protein.
false
false
_98_
0
2407
98
Not in stock
false
This part was not designed with compliance to biobrick standard assembly in mind.
false
Dan Widmaier
annotation1959615
1
sopE2 Secretion Signal
range1959615
1
1
315
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation1701
1
RBS-1\Strong
range1701
1
1
15
BBa_J64009_sequence
1
atgcaacatttggatatcgctgaattagttcgttccgcactggaagtaagtggttgcgatccttcattaattggaggaatagatagccattcaacaattgttctggatttatttgcattgccaagtatctgtatcagcgtcaaggacgatgatgtatggatctgggcgcaattgggtgctgacagcatggtggtattacaacagcgggcttatgaaatcttaatgaccataatggaaggatgccattttgcccgcggcgggcaattactactgggggagcagaatggggagctaacgcttaaagccttagtgcatccggattttttatctgacggtgaaaagttctctactgccttgaatgggttttacaactatctggaagtttttagtcggtcgctaatgagataa
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_J64016_sequence
1
atgactaacataacactatccacccagcactacagaatccatagaagtgacgttgaaccagtaaaagaaaaaacaacggagaaggacatttttgcaaaaagtattactgccgttagaaatagctttatcagcctgtcgacgagtctgtcagatcgttttagcctgcatcaacaaacagacataccgactacccattttcatcgtgggaacgcttctgagggtagggcggtattaaccagtaaaactgttaaagattttatgctgcaaaagctcaatagtctggatatcaaaggtaatgcgagtaaagatccggcc
BBa_J64038_sequence
1
ttaattaaggaaaagatctatgcaacatttggatatcgctgaattagttcgttccgcactggaagtaagtggttgcgatccttcattaattggaggaatagatagccattcaacaattgttctggatttatttgcattgccaagtatctgtatcagcgtcaaggacgatgatgtatggatctgggcgcaattgggtgctgacagcatggtggtattacaacagcgggcttatgaaatcttaatgaccataatggaaggatgccattttgcccgcggcgggcaattactactgggggagcagaatggggagctaacgcttaaagccttagtgcatccggattttttatctgacggtgaaaagttctctactgccttgaatgggttttacaactatctggaagtttttagtcggtcgctaatgagataaattaaagaggagaaaatgactaacataacactatccacccagcactacagaatccatagaagtgacgttgaaccagtaaaagaaaaaacaacggagaaggacatttttgcaaaaagtattactgccgttagaaatagctttatcagcctgtcgacgagtctgtcagatcgttttagcctgcatcaacaaacagacataccgactacccattttcatcgtgggaacgcttctgagggtagggcggtattaaccagtaaaactgttaaagattttatgctgcaaaagctcaatagtctggatatcaaaggtaatgcgagtaaagatccggcc
BBa_J64011_sequence
1
ttaattaaggaaaagatct
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z