BBa_J64022
1
BBa_J64022
sicA RBS
2008-01-16T12:00:00Z
2015-08-31T01:56:26Z
Salmonella LT2 Genomic DNA
This is the natural RBS from the sicA chaperone (BBa_J6021) in the Salmonella Type III Secretion System.
false
false
_98_
0
2407
98
Not in stock
false
This part was not designed with standard biobrick assembly in mind.
false
Dan Widmaier
annotation1959621
1
misc
range1959621
1
1
20
BBa_J64024
1
BBa_J64024
sipC RBS
2008-01-16T12:00:00Z
2015-08-31T01:56:26Z
Salmonella LT2 Genomic DNA
This part is the natural RBS from the sipC protein in Salmonella Type III Secretion.
false
false
_98_
0
2407
98
Not in stock
false
This part was not designed with biobrick standard assembly in mind. This RBS has not been explicitly measured for expression rate using fluorescence or B-Gal assay.
false
Dan Widmaier
annotation1959623
1
sipC RBS
range1959623
1
1
15
BBa_J64040
1
BBa_J64040
sicA Chaperone sipC Tag
2008-01-16T12:00:00Z
2015-05-08T01:08:16Z
This part is comprised of parts taken from Salmonella LT2 Genomic DNA
This is a variant of the pCASP secretion circuit with the sicA chaperone and the sipC secretion signal.
false
false
_98_
0
2407
98
Not in stock
false
This part was not designed with biobrick standard assembly compatibility in mind, it might contain scar sequence cutsites.
false
Dan Widmaier
component1960366
1
BBa_J64021
component1960364
1
BBa_J64022
component1960368
1
BBa_J64024
component1960370
1
BBa_J64023
annotation1960366
1
BBa_J64021
range1960366
1
21
518
annotation1960368
1
BBa_J64024
range1960368
1
519
533
annotation1960364
1
BBa_J64022
range1960364
1
1
20
annotation1960370
1
BBa_J64023
range1960370
1
534
896
BBa_J64023
1
BBa_J64023
sipC Secretion Signal
2008-01-16T12:00:00Z
2015-08-31T01:56:26Z
Salmonella LT2 Genomic DNA
This part is the 167 amino acid secretion signal for the sipC protein in the Salmonella Type III Secretion System. This secretion signal can be fused to proteins of interest and will direct secretion to the extracellular space via the type III secretion apparatus. For secretion at high titer co-expression of the sicA chaperone protein (BBa_J64021) is required.
false
false
_98_
0
2407
98
Not in stock
false
This part was not designed with compliance to biobricks standard assembly in mind.
false
Dan Widmaier
annotation1959622
1
sipC Secretion Signal
range1959622
1
1
363
BBa_J64021
1
BBa_J64021
sicA Chaperone
2008-01-16T12:00:00Z
2015-08-31T01:56:26Z
Salmonella Typhimurium LT2 Genomic DNA.
This part is a secretion chaperone from the Salmonella Type III Secretion System (T3S). SicA is required for secretion of sipC protein or sipC tagged heterologous proteins. SicA also functions as a component of a positive feedback loop involving the sicA promoter and invF. For more information see Temme et al in the reference section.
false
false
_98_
0
2407
98
Not in stock
false
This part was not designed with biobrick standard assembly in mind.
false
Dan Widmaier
annotation1959620
1
sicA
range1959620
1
1
498
BBa_J64040_sequence
1
acagataacaggagtaagtaatggattatcaaaataatgtcagcgaagaacgtgttgcggaaatgatttgggatgccgttagtgaaggcgccacgctaaaagacgttcatgggatccctcaagatatgatggacggtttatatgctcatgcttatgagttttataaccagggacgactggatgaagctgagacgttctttcgtttcttatgcatttatgatttttacaatcccgattacaccatgggactggcggcagtatgccaactgaaaaaacaatttcagaaagcatgtgacctttatgcagtagcgtttacgttacttaaaaatgattatcgccccgttttttttaccgggcagtgtcaattattaatgcgtaaggcagcaaaagccagacagtgttttgaacttgtcaatgaacgtactgaagatgagtctctgcgggcaaaagcgttggtctatctggaggcgctaaaaacggcggagacagagcagcacagtgaacaagaaaaggaataataaagggagaaaaatatgttaattagtaatgtgggaataaatcccgccgcttatttaaataatcattctgttgagaatagttcacagacagcttcgcaatccgttagcgctaaagatattctgaatagtattggtattagcagcagtaaagtcagtgacctggggttgagtcctacactgagcgcgcctgcgccaggggtattaacgcaaacccccggaacgatcacgtcctttttaaaagccagtattcaaaataccgacatgaatcaggatttgaatgctctggcaaataatgtcacgactaaagcgaatgaggttgtgcaaacccagttacgcgagcagcaggcagaagtcggaaagttttttgatattagcgga
BBa_J64024_sequence
1
taaagggagaaaaat
BBa_J64021_sequence
1
atggattatcaaaataatgtcagcgaagaacgtgttgcggaaatgatttgggatgccgttagtgaaggcgccacgctaaaagacgttcatgggatccctcaagatatgatggacggtttatatgctcatgcttatgagttttataaccagggacgactggatgaagctgagacgttctttcgtttcttatgcatttatgatttttacaatcccgattacaccatgggactggcggcagtatgccaactgaaaaaacaatttcagaaagcatgtgacctttatgcagtagcgtttacgttacttaaaaatgattatcgccccgttttttttaccgggcagtgtcaattattaatgcgtaaggcagcaaaagccagacagtgttttgaacttgtcaatgaacgtactgaagatgagtctctgcgggcaaaagcgttggtctatctggaggcgctaaaaacggcggagacagagcagcacagtgaacaagaaaaggaataa
BBa_J64023_sequence
1
atgttaattagtaatgtgggaataaatcccgccgcttatttaaataatcattctgttgagaatagttcacagacagcttcgcaatccgttagcgctaaagatattctgaatagtattggtattagcagcagtaaagtcagtgacctggggttgagtcctacactgagcgcgcctgcgccaggggtattaacgcaaacccccggaacgatcacgtcctttttaaaagccagtattcaaaataccgacatgaatcaggatttgaatgctctggcaaataatgtcacgactaaagcgaatgaggttgtgcaaacccagttacgcgagcagcaggcagaagtcggaaagttttttgatattagcgga
BBa_J64022_sequence
1
acagataacaggagtaagta
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z