LOCUS       BBa_J64100              2800 bp    DNA     linear       22-Dec-2024
DEFINITION  Medium copy ColE1 BioBrick vector
ACCESSION   BBa_J64100
VERSION     BBa_J64100.1  
FEATURES             Location/Qualifiers
     primer_bind     6..25
                     /label=G00100
     misc_feature    124..145
                     /label=prefix
     misc_feature    146..820
                     /label=P1010
     misc_feature    821..841
                     /label=suffix
     primer_bind     977..996
                     /label=G00101
     misc_feature    1130..1812
                     /label=ColE1 origin
     misc_feature    1939..2598
                     /label=chloramphenicol resistance marker
     primer_bind     1029..1048
                     /label=G00101
ORIGIN
        1 tcgagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
       61 gcgtatcacg aggcagaatt tcagataaaa aaaatcctta gctttcgcta aggatgattt
      121 ctggaattcg cggccgcttc tagagactgg ctgtgtataa gggagcctga catttatatt
      181 ccccagaaca tcaggttaat ggcgtttttg atgtcatttt cgcggtggct gagatcagcc
      241 acttcttccc cgataacgga gaccggcaca ctggccatat cggtggtcat catgcgccag
      301 ctttcatccc cgatatgcac caccgggtaa agttcacggg agactttatc tgacagcaga
      361 cgtgcactgg ccagggggat caccatccgt cgcccgggcg tgtcaataat atcactctgt
      421 acatccacaa acagacgata acggctctct cttttatagg tgtaaacctt aaactgcatt
      481 tcaccagccc ctgttctcgt cagcaaaaga gccgttcatt tcaataaacc gggcgacctc
      541 agccatccct tcctgatttt ccgctttcca gcgttcggca cgcagacgac gggcttcatt
      601 ctgcatggtt gtgcttacca gaccggagat attgacatca tatatgcctt gagcaactga
      661 tagctgtcgc tgtcaactgt cactgtaata cgctgcttca tagcatacct ctttttgaca
      721 tacttcgggt atacatatca gtatatattc ttataccgca aaaatcagcg cgcaaatacg
      781 catactgtta tctggctttt agtaagccgg atccacgcgt tactagtagc ggccgctgca
      841 gtccggcaaa aaaacgggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag
      901 attacttcgc gttatgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      961 gcggcgagcg gtatcagctc actcaaaggc ggtaatccta gggcgttcgg ctgcggcgag
     1021 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     1081 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     1141 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     1201 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     1261 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     1321 cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg
     1381 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1441 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     1501 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     1561 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     1621 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     1681 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     1741 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     1801 ttttggtcat gacttgtgct tggattctca ccaataaaaa acgcccggcg gcaaccgagc
     1861 gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca
     1921 agcgagctcg atatcaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc
     1981 attaagcatt ctgccgacat ggaagccatc acagacggca tgatgaacct gaatcgccag
     2041 cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa
     2101 gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc
     2161 tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta
     2221 acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact
     2281 ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact
     2341 atcccatatc accagctcac cgtctttcat tgccatacga aattccggat gagcattcat
     2401 caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt
     2461 ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga
     2521 ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc
     2581 agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa
     2641 tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc
     2701 aacgtctcat tttcgccaga tatcgacgtc taagaaacca ttattatcat gacattaacc
     2761 tataaaaata ggcgtatcac gaggcccttt cgtcttcacc
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