LOCUS       BBa_J64101              2254 bp    DNA     linear       24-Dec-2024
DEFINITION  ColE1 biobrick plasmid with backward terminator
ACCESSION   BBa_J64101
VERSION     BBa_J64101.1  
FEATURES             Location/Qualifiers
     misc_feature    1..2800
                     /label=BBa_J64100
     primer_bind     6..25
                     /label=G00100
     misc_feature    124..145
                     /label=prefix
     misc_feature    146..274
                     /label=B0025
     misc_feature    275..295
                     /label=suffix
     primer_bind     431..450
                     /label=G00101
     primer_bind     483..502
                     /label=G00101
ORIGIN
        1 tcgagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
       61 gcgtatcacg aggcagaatt tcagataaaa aaaatcctta gctttcgcta aggatgattt
      121 ctggaattcg cggccgcttc tagagtataa acgcagaaag gcccacccga aggtgagcca
      181 gtgtgactct agtagagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct
      241 ttcgactgag cctttcgttt tatttgatgc ctggtactag tagcggccgc tgcagtccgg
      301 caaaaaaacg ggcaaggtgt caccaccctg ccctttttct ttaaaaccga aaagattact
      361 tcgcgttatg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
      421 agcggtatca gctcactcaa aggcggtaat cctagggcgt tcggctgcgg cgagcggtat
      481 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      541 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      601 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      661 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      721 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      781 gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      841 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      901 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      961 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     1021 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     1081 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     1141 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     1201 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     1261 tcatgacttg tgcttggatt ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg
     1321 aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag
     1381 ctcgatatca aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag
     1441 cattctgccg acatggaagc catcacagac ggcatgatga acctgaatcg ccagcggcat
     1501 cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt
     1561 gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac
     1621 gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc
     1681 cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag
     1741 cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca
     1801 tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg
     1861 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa
     1921 aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa
     1981 tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat
     2041 ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc
     2101 cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc
     2161 tcattttcgc cagatatcga cgtctaagaa accattatta tcatgacatt aacctataaa
     2221 aataggcgta tcacgaggcc ctttcgtctt cacc
//