LOCUS       BBa_J64713              1661 bp    DNA     linear       18-May-2024
DEFINITION  LasR/LasI Inducible (RHLR/RHLI repressible) positive feedback 
            production of 3OC12 HSL
ACCESSION   BBa_J64713
VERSION     BBa_J64713.1  
FEATURES             Location/Qualifiers
     misc_feature    46..64
                     /label=LasR/LasI O2
     misc_feature    106..125
                     /label=LasR/LasI O1
     misc_feature    125..141
                     /label=RHLR/RHLI
     misc_feature    117..122
                     /label=-35
     misc_feature    140..145
                     /label=-10
     promoter        1..157
                     /label=BBa_J64712
     misc_feature    170..173
                     /label=conserved
     RBS             166..177
                     /label=BBa_B0034
     CDS             184..792
                     /label=lasI
     CDS             184..792
                     /label=BBa_C0178
     misc_feature    805..808
                     /label=conserved
     RBS             801..812
                     /label=BBa_B0034
     CDS             819..1493
                     /label=mrfp1
     misc_feature    1500..1524
                     /label=Help:Barcodes
     CDS             819..1524
                     /label=BBa_E1010
     stem_loop       1544..1587
                     /label=stem_loop
     misc_feature    1533..1612
                     /label=BBa_B0010
     terminator      1533..1612
                     /label=BBa_B0010
     stem_loop       1628..1647
                     /label=T7 TE
     misc_feature    1654..1654
                     /label=stop
     polyA_site      1648..1661
                     /label=polya
     misc_feature    1621..1661
                     /label=BBa_B0012
     terminator      1621..1661
                     /label=BBa_B0012
     terminator      1533..1661
                     /label=BBa_B0015
ORIGIN
        1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct
       61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg
      121 caggtgtgaa atctggcagt ttttggtaca cgaaagctac tagagaaaga ggagaaatac
      181 tagatgatcg ttcagatcgg tcgtcgtgaa gagttcgaca aaaaactgct gggtgaaatg
      241 cacaaactgc gtgctcaggt tttcaaagaa cgtaaaggtt gggacgtttc cgttatcgac
      301 gaaatggaaa tcgacggtta cgacgctctg tccccgtact acatgctgat ccaggaagac
      361 accccggaag ctcaggtttt cggttgctgg cgtatcttcg acaccaccgg tccgtacatg
      421 ctgaaaaaca ccttcccgga actgctgcac ggtaaagaag ctccgtgctc cccgcacatc
      481 tgggaactgt cccgtttcgc tatcaactcc ggtcagaaag gttccctggg tttctccgac
      541 tgcaccctgg aagctatgcg tgctctggct cgttactcct tgcagaacga catccagacc
      601 ctggttaccg ttaccaccgt tggtgttgaa aaaatgatga tccgtgctgg tctggacgtt
      661 tcccgtttcg gtccgcacct gaaaatcggt atcgaacgtg ctgttgctct gcgtatcgaa
      721 ctgaacgcta aaacccagat cgctctgtac ggtggtgttc tggttgaaca gcgtctggct
      781 gtttcctaat aatactagag aaagaggaga aatactagat ggcttcctcc gaagacgtta
      841 tcaaagagtt catgcgtttc aaagttcgta tggaaggttc cgttaacggt cacgagttcg
      901 aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag
      961 ttaccaaagg tggtccgctg ccgttcgctt gggacatcct gtccccgcag ttccagtacg
     1021 gttccaaagc ttacgttaaa cacccggctg acatcccgga ctacctgaaa ctgtccttcc
     1081 cggaaggttt caaatgggaa cgtgttatga acttcgaaga cggtggtgtt gttaccgtta
     1141 cccaggactc ctccctgcaa gacggtgagt tcatctacaa agttaaactg cgtggtacca
     1201 acttcccgtc cgacggtccg gttatgcaga aaaaaaccat gggttgggaa gcttccaccg
     1261 aacgtatgta cccggaagac ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga
     1321 aagacggtgg tcactacgac gctgaagtta aaaccaccta catggctaaa aaaccggttc
     1381 agctgccggg tgcttacaaa accgacatca aactggacat cacctcccac aacgaagact
     1441 acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt gcttaataac
     1501 gctgatagtg ctagtgtaga tcgctactag agccaggcat caaataaaac gaaaggctca
     1561 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     1621 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//