BBa_J64713
type: ComponentDefinition
persistent identity: BBa J64713
display id: BBa_J64713
version: 1
was generated by: iGEM to SBOL conversion
description: LasR/LasI Inducible (RHLR/RHLI repressible) positive feedback production of 3OC12 HSL
created: 2007-03-24T12:00:00Z
modified: 2015-05-08T01:08:17Z
mutable provenance: For source of the promoter see j64712. The rest of the parts existed in the registry already.
owned by: james
owned by: myers
mutable description: This construct will produce more 3OC12 HSL when the circuit is given an inital amount of 3OC12 and when LasR is present in the system. This construct will also produce RFP as a reporter when expressed.
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1431
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: project goals
star: false
creator: laura lavery
type: DnaRegion
role: partType/Project
role: SO:0000804
component: BBa_B0015
component: BBa_E1010
component: BBa_B0034
component: BBa_C0178
component: BBa_J64712
sequence annotation: BBa_J64712
sequence annotation: BBa_B0034
sequence annotation: BBa_C0178
sequence annotation: BBa_E1010
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_J64713_sequence/1

BBa_B0015
type: Component
persistent identity: component2265280
display id: component2265280
version: 1
top level: BBa_J64713
definition: BBa_B0015
access: public

BBa_E1010
type: Component
persistent identity: component2265273
display id: component2265273
version: 1
top level: BBa_J64713
definition: mRFP1
access: public

BBa_B0034
type: Component
persistent identity: component2265270
display id: component2265270
version: 1
top level: BBa_J64713
definition: BBa_B0034
access: public

BBa_C0178
type: Component
persistent identity: component2265268
display id: component2265268
version: 1
top level: BBa_J64713
definition: lasI
access: public

BBa_B0034
type: Component
persistent identity: component2265266
display id: component2265266
version: 1
top level: BBa_J64713
definition: BBa_B0034
access: public

BBa_J64712
type: Component
persistent identity: component2265264
display id: component2265264
version: 1
top level: BBa_J64713
definition: BBa_J64712
access: public

BBa_J64712
type: SequenceAnnotation
persistent identity: annotation2265264
display id: annotation2265264
version: 1
top level: BBa_J64713
location: https://synbiohub.org/public/igem/BBa_J64713/annotation2265264/range2265264/1
component: BBa_J64712

https://synbiohub.org/public/igem/BBa_J64713/annotation2265264/range2265264/1
type: Range
persistent identity: range2265264
display id: range2265264
version: 1
top level: BBa_J64713
direction: direction/reverse
start: 1
end: 157
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2265266
display id: annotation2265266
version: 1
top level: BBa_J64713
location: https://synbiohub.org/public/igem/BBa_J64713/annotation2265266/range2265266/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J64713/annotation2265266/range2265266/1
type: Range
persistent identity: range2265266
display id: range2265266
version: 1
top level: BBa_J64713
direction: direction/reverse
start: 166
end: 177
orientation: inline

BBa_C0178
type: SequenceAnnotation
persistent identity: annotation2265268
display id: annotation2265268
version: 1
top level: BBa_J64713
location: https://synbiohub.org/public/igem/BBa_J64713/annotation2265268/range2265268/1
component: BBa_C0178

https://synbiohub.org/public/igem/BBa_J64713/annotation2265268/range2265268/1
type: Range
persistent identity: range2265268
display id: range2265268
version: 1
top level: BBa_J64713
direction: direction/reverse
start: 184
end: 792
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2265270
display id: annotation2265270
version: 1
top level: BBa_J64713
location: https://synbiohub.org/public/igem/BBa_J64713/annotation2265270/range2265270/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J64713/annotation2265270/range2265270/1
type: Range
persistent identity: range2265270
display id: range2265270
version: 1
top level: BBa_J64713
direction: direction/reverse
start: 801
end: 812
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2265273
display id: annotation2265273
version: 1
top level: BBa_J64713
location: https://synbiohub.org/public/igem/BBa_J64713/annotation2265273/range2265273/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_J64713/annotation2265273/range2265273/1
type: Range
persistent identity: range2265273
display id: range2265273
version: 1
top level: BBa_J64713
direction: direction/reverse
start: 819
end: 1524
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2265280
display id: annotation2265280
version: 1
top level: BBa_J64713
location: https://synbiohub.org/public/igem/BBa_J64713/annotation2265280/range2265280/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_J64713/annotation2265280/range2265280/1
type: Range
persistent identity: range2265280
display id: range2265280
version: 1
top level: BBa_J64713
direction: direction/reverse
start: 1533
end: 1661
orientation: inline

BBa_J64712
type: ComponentDefinition
persistent identity: BBa J64712
display id: BBa_J64712
version: 1
was generated by: iGEM to SBOL conversion
description: LasR/LasI Inducible & RHLR/RHLI repressible Promoter
created: 2007-03-24T12:00:00Z
modified: 2015-05-08T01:08:17Z
mutable provenance: Rust L. et. al., "Analysis of the Pseudomonas aeruginosa Elastase (lasB) Regulatory Region". Journal of Bacteriology, 1996.
owned by: james
owned by: myers
mutable description: This part is similar to BBa_R0079 but has an added binding site for RHLR/RHLI in between the -35 and -10 region. Thus this promoter is induced by LasR/LasI but repressed by RHLR/RHLI binding.
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1431
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The natural spacer between the -35 and -10 is 14bp and the natural O1 site for RHLR/RHLI is 20bp long. There was not much over lap of the -35 or the -10 with this sequence, so it was nessicary to chop off some of the binding site sequence in order to maintain the correct spacing and preserve the -35 and -10 sequence. The binding site sequence is missing a tcc on is 5' end and a t on its 3' end.
star: false
creator: laura lavery
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LasR/LasI O2
sequence annotation: LasR/LasI O1
sequence annotation: -35
sequence annotation: RHLR/RHLI
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_J64712_sequence/1

LasR/LasI O2
type: SequenceAnnotation
persistent identity: annotation1921064
display id: annotation1921064
version: 1
top level: BBa_J64712
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921064/range1921064/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_J64712/annotation1921064/range1921064/1
type: Range
persistent identity: range1921064
display id: range1921064
version: 1
top level: BBa_J64712
direction: direction/forward
start: 46
end: 64
orientation: inline

LasR/LasI O1
type: SequenceAnnotation
persistent identity: annotation1921065
display id: annotation1921065
version: 1
top level: BBa_J64712
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921065/range1921065/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_J64712/annotation1921065/range1921065/1
type: Range
persistent identity: range1921065
display id: range1921065
version: 1
top level: BBa_J64712
direction: direction/forward
start: 106
end: 125
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1921067
display id: annotation1921067
version: 1
top level: BBa_J64712
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921067/range1921067/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J64712/annotation1921067/range1921067/1
type: Range
persistent identity: range1921067
display id: range1921067
version: 1
top level: BBa_J64712
direction: direction/forward
start: 117
end: 122
orientation: inline

RHLR/RHLI
type: SequenceAnnotation
persistent identity: annotation1921066
display id: annotation1921066
version: 1
top level: BBa_J64712
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921066/range1921066/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_J64712/annotation1921066/range1921066/1
type: Range
persistent identity: range1921066
display id: range1921066
version: 1
top level: BBa_J64712
direction: direction/forward
start: 125
end: 141
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1921068
display id: annotation1921068
version: 1
top level: BBa_J64712
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921068/range1921068/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J64712/annotation1921068/range1921068/1
type: Range
persistent identity: range1921068
display id: range1921068
version: 1
top level: BBa_J64712
direction: direction/forward
start: 140
end: 145
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

lasI
type: ComponentDefinition
persistent identity: BBa C0178
display id: BBa_C0178
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer synthetase for PAI from Pseudomonas aeruginosa (no LVA)
created: 2004-05-26T11:00:00Z
modified: 2015-08-31T04:07:24Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: same as C0078 except no LVA tag
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: lasI
sequence: https://synbiohub.org/public/igem/BBa_C0178_sequence/1

lasI
type: SequenceAnnotation
persistent identity: annotation1937124
display id: annotation1937124
version: 1
top level: lasI
location: https://synbiohub.org/public/igem/BBa_C0178/annotation1937124/range1937124/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0178/annotation1937124/range1937124/1
type: Range
persistent identity: range1937124
display id: range1937124
version: 1
top level: lasI
direction: direction/forward
start: 1
end: 609
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_J64713_sequence/1
type: Sequence
persistent identity: BBa J64713 sequence
display id: BBa_J64713_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaaggctacctgccagttctggcaggtgtgaaatctggcagtttttggtacacgaaagctactagagaaagaggagaaatactagatgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttcctaataatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J64712_sequence/1
type: Sequence
persistent identity: BBa J64712 sequence
display id: BBa_J64712_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaaggctacctgccagttctggcaggtgtgaaatctggcagtttttggtacacgaaagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0178_sequence/1
type: Sequence
persistent identity: BBa C0178 sequence
display id: BBa_C0178_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttcctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z