LOCUS BBa_J64717 5132 bp DNA linear 27-Dec-2024 DEFINITION Self-assembled Pattern Formation Generator ACCESSION BBa_J64717 VERSION BBa_J64717.1 FEATURES Location/Qualifiers misc_feature 46..64 /label=LasR/LasI O2 misc_feature 106..125 /label=LasR/LasI O1 misc_feature 125..141 /label=RHLR/RHLI misc_feature 117..122 /label=-35 misc_feature 140..145 /label=-10 promoter 1..157 /label=BBa_J64712 misc_feature 170..173 /label=conserved RBS 166..177 /label=BBa_B0034 CDS 184..792 /label=lasI CDS 184..792 /label=BBa_C0178 misc_feature 805..808 /label=conserved RBS 801..812 /label=BBa_B0034 CDS 819..1493 /label=mrfp1 misc_feature 1500..1524 /label=Help:Barcodes CDS 819..1524 /label=BBa_E1010 stem_loop 1544..1587 /label=stem_loop misc_feature 1533..1612 /label=BBa_B0010 terminator 1533..1612 /label=BBa_B0010 stem_loop 1628..1647 /label=T7 TE misc_feature 1654..1654 /label=stop polyA_site 1648..1661 /label=polya misc_feature 1621..1661 /label=BBa_B0012 terminator 1621..1661 /label=BBa_B0012 terminator 1533..1661 /label=BBa_B0015 misc_feature 1..1661 /label=BBa_J64713 misc_feature 1670..1692 /label=RHLR/RHLI misc_feature 1692..1709 /label=LasR/LasI misc_feature 1688..1693 /label=-35 misc_feature 1711..1716 /label=-10 promoter 1670..1722 /label=BBa_J64800 misc_feature 1735..1738 /label=conserved RBS 1731..1742 /label=BBa_B0034 CDS 1749..2357 /label=BBa_J64718 misc_feature 2370..2373 /label=conserved RBS 2366..2377 /label=BBa_B0034 CDS 2384..3103 /label=GFP protein CDS 2384..3103 /label=BBa_E0040 stem_loop 3123..3166 /label=stem_loop misc_feature 3112..3191 /label=BBa_B0010 terminator 3112..3191 /label=BBa_B0010 stem_loop 3207..3226 /label=T7 TE misc_feature 3233..3233 /label=stop polyA_site 3227..3240 /label=polya misc_feature 3200..3240 /label=BBa_B0012 terminator 3200..3240 /label=BBa_B0012 misc_feature 1670..3240 /label=BBa_J64714 promoter 3286..3291 /label=-10 promoter 3263..3268 /label=-35 misc_feature 3273..3289 /label=OR1 misc_feature 3249..3265 /label=OR2 misc_feature 3249..3297 /label=BBa_R0051 promoter 3249..3297 /label=BBa_R0051 misc_feature 3310..3313 /label=conserved RBS 3306..3317 /label=BBa_B0034 CDS 3324..4046 /label=BBa_C0179 stem_loop 4066..4109 /label=stem_loop misc_feature 4055..4134 /label=BBa_B0010 terminator 4055..4134 /label=BBa_B0010 stem_loop 4150..4169 /label=T7 TE misc_feature 4176..4176 /label=stop polyA_site 4170..4183 /label=polya misc_feature 4143..4183 /label=BBa_B0012 terminator 4143..4183 /label=BBa_B0012 misc_feature 3249..4183 /label=BBa_J64715 promoter 4229..4234 /label=-10 promoter 4206..4211 /label=-35 misc_feature 4216..4232 /label=OR1 misc_feature 4192..4208 /label=OR2 misc_feature 4192..4240 /label=BBa_R0051 promoter 4192..4240 /label=BBa_R0051 misc_feature 4253..4256 /label=conserved RBS 4249..4260 /label=BBa_B0034 CDS 4267..4995 /label=BBa_C0171 stem_loop 5015..5058 /label=stem_loop misc_feature 5004..5083 /label=BBa_B0010 terminator 5004..5083 /label=BBa_B0010 stem_loop 5099..5118 /label=T7 TE misc_feature 5125..5125 /label=stop polyA_site 5119..5132 /label=polya misc_feature 5092..5132 /label=BBa_B0012 terminator 5092..5132 /label=BBa_B0012 misc_feature 4192..5132 /label=BBa_J64716 ORIGIN 1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct 61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg 121 caggtgtgaa atctggcagt ttttggtaca cgaaagctac tagagaaaga ggagaaatac 181 tagatgatcg ttcagatcgg tcgtcgtgaa gagttcgaca aaaaactgct gggtgaaatg 241 cacaaactgc gtgctcaggt tttcaaagaa cgtaaaggtt gggacgtttc cgttatcgac 301 gaaatggaaa tcgacggtta cgacgctctg tccccgtact acatgctgat ccaggaagac 361 accccggaag ctcaggtttt cggttgctgg cgtatcttcg acaccaccgg tccgtacatg 421 ctgaaaaaca ccttcccgga actgctgcac ggtaaagaag ctccgtgctc cccgcacatc 481 tgggaactgt cccgtttcgc tatcaactcc ggtcagaaag gttccctggg tttctccgac 541 tgcaccctgg aagctatgcg tgctctggct cgttactcct tgcagaacga catccagacc 601 ctggttaccg ttaccaccgt tggtgttgaa aaaatgatga tccgtgctgg tctggacgtt 661 tcccgtttcg gtccgcacct gaaaatcggt atcgaacgtg ctgttgctct gcgtatcgaa 721 ctgaacgcta aaacccagat cgctctgtac ggtggtgttc tggttgaaca gcgtctggct 781 gtttcctaat aatactagag aaagaggaga aatactagat ggcttcctcc gaagacgtta 841 tcaaagagtt catgcgtttc aaagttcgta tggaaggttc cgttaacggt cacgagttcg 901 aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag 961 ttaccaaagg tggtccgctg ccgttcgctt gggacatcct gtccccgcag ttccagtacg 1021 gttccaaagc ttacgttaaa cacccggctg acatcccgga ctacctgaaa ctgtccttcc 1081 cggaaggttt caaatgggaa cgtgttatga acttcgaaga cggtggtgtt gttaccgtta 1141 cccaggactc ctccctgcaa gacggtgagt tcatctacaa agttaaactg cgtggtacca 1201 acttcccgtc cgacggtccg gttatgcaga aaaaaaccat gggttgggaa gcttccaccg 1261 aacgtatgta cccggaagac ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga 1321 aagacggtgg tcactacgac gctgaagtta aaaccaccta catggctaaa aaaccggttc 1381 agctgccggg tgcttacaaa accgacatca aactggacat cacctcccac aacgaagact 1441 acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt gcttaataac 1501 gctgatagtg ctagtgtaga tcgctactag agccaggcat caaataaaac gaaaggctca 1561 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 1621 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagt cctgtgaaat 1681 ctggcagtta cctgccagtt ctggcaggtc taaaaagtgt tctactagag aaagaggaga 1741 aatactagat gatcgaactg ctgtccgaat ccctggaagg tctgtccgct gctatgatcg 1801 ctgaactggg tcgttaccgt caccaggttt tcatcgaaaa actgggttgg gacgttgttt 1861 ccacctcccg tgttcgtgac caggagttcg accagttcga ccacccgcag acccgttaca 1921 tcgttgctat gtcccgtcag ggtatctgcg gttgcgctcg tctgctgccg accaccgacg 1981 cttacctgct gaaagacgtt ttcgcttacc tgtgctccga aaccccgccg tccgacccgt 2041 ccgtttggga actgtcccgt tacgctgctt ccgctgctga cgacccgcag ctggctatga 2101 aaatcttctg gtcctccctc cagtgcgctt ggtacctggg tgcttcctcc gttgttgctg 2161 ttaccaccac cgctatggaa cgttacttcg ttcgtaacgg tgttatcctc cagcgtctgg 2221 gtccgccgca gaaagttaaa ggtgaaaccc tggttgctat ctccttcccg gcttaccagg 2281 aacgtggtct ggaaatgctg ctgcgttacc acccggaatg gctccagggt gttccgctgt 2341 ccatggctgt ttaataatac tagagaaaga ggagaaatac tagatgcgta aaggagaaga 2401 acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa 2461 attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt 2521 tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcggtta 2581 tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag 2641 tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta 2701 caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa 2761 aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa 2821 ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa 2881 aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac 2941 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc 3001 cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc 3061 tgctgggatt acacatggca tggatgaact atacaaataa taatactaga gccaggcatc 3121 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 3181 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 3241 tactagagta acaccgtgcg tgttgactat tttacctctg gcggtgataa tggttgctac 3301 tagagaaaga ggagaaatac tagatggcct tggttgacgg ttttcttgag ctggaacgct 3361 caagtggaaa attggagtgg agcgccatcc tccagaagat ggcgagcgac cttggattct 3421 cgaagatcct gttcggcctg ttgcctaagg acagccagga ctacgagaac gccttcatcg 3481 tcggcaacta cccggccgcc tggcgcgagc attacgaccg ggctggctac gcgcgggtcg 3541 acccgacggt cagtcactgt acccagagcg tactgccgat tttctgggaa ccgtccatct 3601 accagacgcg aaagcagcac gagttcttcg aggaagcctc ggccgccggc ctggtgtatg 3661 ggctgaccat gccgctgcat ggtgctcgcg gcgaactcgg cgcgctgagc ctcagcgtgg 3721 aagcggaaaa ccgggccgag gccaaccgtt tcatagagtc ggtcctgccg accctgtgga 3781 tgctcaagga ctacgcactg caaagcggtg ccggactggc cttcgaacat ccggtcagca 3841 aaccggtggt tctgaccagc cgggagaagg aagtgttgca gtggtgcgcc atcggcaaga 3901 ccagttggga gatatcggtt atctgcaact gctcggaagc caatgtgaac ttccatatgg 3961 gaaatattcg gcggaagttc ggtgtgacct cccgccgcgt agcggccatt atggccgtta 4021 atttgggtct tattactctc taataatact agagccaggc atcaaataaa acgaaaggct 4081 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 4141 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gtaacaccgt 4201 gcgtgttgac tattttacct ctggcggtga taatggttgc tactagagaa agaggagaaa 4261 tactagatga ggaatgacgg aggctttttg ctgtggtggg acggtttgcg tagcgagatg 4321 cagccgatcc acgacagcca gggcgtgttc gccgtcctgg aaaaggaagt gcggcgcctg 4381 ggcttcgatt actacgccta tggcgtgcgc cacacgattc ccttcacccg gccgaagacc 4441 gaggtccatg gcacctatcc caaggcctgg ctggagcgat accagatgca gaactacggg 4501 gccgtggatc cggcgatcct caacggcctg cgctcctcgg aaatggtggt ctggagcgac 4561 agcctgttcg accagagccg gatgctctgg aacgaggctc gcgattgggg cctctgtgtc 4621 ggcgcgacct tgccgatccg cgcgccgaac aatttgctca gcgtgctttc cgtggcgcgc 4681 gaccagcaga acatctccag cttcgagcgc gaggaaatcc gcctgcggct gcgttgcatg 4741 atcgagttgc tgacccagaa gctgaccgac ctggagcatc cgatgctgat gtccaacccg 4801 gtctgcctga gccatcgcga acgcgagatc ctgcaatgga ccgccgacgg caagagttcc 4861 ggggaaatcg ccatcatcct gagcatctcc gagagcacgg tgaacttcca ccacaagaac 4921 atccagaaga agttcgacgc gccgaacaag acgctggctg ccgcctacgc cgcggcgctg 4981 ggtctcatct aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 5041 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 5101 ctcaccttcg ggtgggcctt tctgcgttta ta //