##gff-version 3
##sequence-region BBa_J64717 1 5132
BBa_J64717	.	engineered_region	3249	4183	.	+	0	ID=BBa_J64715;Name=BBa_J64715
BBa_J64717	.	promoter	3249	3297	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_J64715
BBa_J64717	.	promoter	3286	3291	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_J64717	.	non_covalent_binding_site	3273	3289	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_J64717	.	engineered_region	3249	3297	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_J64717	.	non_covalent_binding_site	3249	3265	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_J64717	.	promoter	3263	3268	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_J64717	.	CDS	3324	4046	.	+	0	ID=BBa_C0179;Name=BBa_C0179;Parent=BBa_J64715
BBa_J64717	.	ribosome_entry_site	3306	3317	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J64715
BBa_J64717	.	conserved_region	3310	3313	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J64717	.	terminator	4055	4134	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J64715
BBa_J64717	.	engineered_region	4055	4134	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J64717	.	stem_loop	4066	4109	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J64717	.	terminator	4143	4183	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J64715
BBa_J64717	.	engineered_region	4143	4183	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J64717	.	stem_loop	4150	4169	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J64717	.	polyA_site	4170	4183	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J64717	.	stop_codon	4176	4176	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J64717	.	engineered_region	4192	5132	.	+	0	ID=BBa_J64716;Name=BBa_J64716
BBa_J64717	.	terminator	5004	5083	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J64716
BBa_J64717	.	engineered_region	5004	5083	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J64717	.	stem_loop	5015	5058	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J64717	.	terminator	5092	5132	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J64716
BBa_J64717	.	engineered_region	5092	5132	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J64717	.	stem_loop	5099	5118	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J64717	.	polyA_site	5119	5132	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J64717	.	stop_codon	5125	5125	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J64717	.	promoter	4192	4240	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_J64716
BBa_J64717	.	promoter	4229	4234	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_J64717	.	non_covalent_binding_site	4216	4232	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_J64717	.	engineered_region	4192	4240	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_J64717	.	non_covalent_binding_site	4192	4208	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_J64717	.	promoter	4206	4211	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_J64717	.	CDS	4267	4995	.	+	0	ID=BBa_C0171;Name=BBa_C0171;Parent=BBa_J64716
BBa_J64717	.	ribosome_entry_site	4249	4260	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J64716
BBa_J64717	.	conserved_region	4253	4256	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J64717	.	engineered_region	1	1661	.	+	0	ID=BBa_J64713;Name=BBa_J64713
BBa_J64717	.	promoter	1	157	.	+	0	ID=BBa_J64712;Name=BBa_J64712;Parent=BBa_J64713
BBa_J64717	.	non_covalent_binding_site	125	141	.	+	0	ID=annotation1921066;Name=RHLR/RHLI;Parent=BBa_J64712
BBa_J64717	.	sequence_feature	140	145	.	+	0	ID=annotation1921068;Name=-10;Parent=BBa_J64712
BBa_J64717	.	sequence_feature	117	122	.	+	0	ID=annotation1921067;Name=-35;Parent=BBa_J64712
BBa_J64717	.	operator	46	64	.	+	0	ID=annotation1921064;Name=LasR/LasI O2;Parent=BBa_J64712
BBa_J64717	.	operator	106	125	.	+	0	ID=annotation1921065;Name=LasR/LasI O1;Parent=BBa_J64712
BBa_J64717	.	CDS	819	1524	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J64713
BBa_J64717	.	CDS	819	1493	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_J64717	.	engineered_tag	1500	1524	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_J64717	.	ribosome_entry_site	166	177	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J64713
BBa_J64717	.	conserved_region	170	173	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J64717	.	terminator	1533	1661	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_J64713
BBa_J64717	.	terminator	1621	1661	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J64717	.	engineered_region	1621	1661	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J64717	.	stem_loop	1628	1647	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J64717	.	polyA_site	1648	1661	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J64717	.	stop_codon	1654	1654	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J64717	.	terminator	1533	1612	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J64717	.	engineered_region	1533	1612	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J64717	.	stem_loop	1544	1587	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J64717	.	CDS	184	792	.	+	0	ID=BBa_C0178;Name=BBa_C0178;Parent=BBa_J64713
BBa_J64717	.	CDS	184	792	.	+	0	ID=annotation1937124;Name=lasI;Parent=BBa_C0178
BBa_J64717	.	ribosome_entry_site	801	812	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J64713
BBa_J64717	.	conserved_region	805	808	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J64717	.	engineered_region	1670	3240	.	+	0	ID=BBa_J64714;Name=BBa_J64714
BBa_J64717	.	ribosome_entry_site	1731	1742	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J64714
BBa_J64717	.	conserved_region	1735	1738	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J64717	.	promoter	1670	1722	.	+	0	ID=BBa_J64800;Name=BBa_J64800;Parent=BBa_J64714
BBa_J64717	.	sequence_feature	1711	1716	.	+	0	ID=annotation1921063;Name=-10;Parent=BBa_J64800
BBa_J64717	.	operator	1692	1709	.	+	0	ID=annotation1921061;Name=LasR/LasI;Parent=BBa_J64800
BBa_J64717	.	sequence_feature	1688	1693	.	+	0	ID=annotation1921062;Name=-35;Parent=BBa_J64800
BBa_J64717	.	non_covalent_binding_site	1670	1692	.	+	0	ID=annotation1921060;Name=RHLR/RHLI;Parent=BBa_J64800
BBa_J64717	.	CDS	2384	3103	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_J64714
BBa_J64717	.	CDS	2384	3103	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J64717	.	ribosome_entry_site	2366	2377	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J64714
BBa_J64717	.	conserved_region	2370	2373	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J64717	.	terminator	3200	3240	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J64714
BBa_J64717	.	engineered_region	3200	3240	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J64717	.	stem_loop	3207	3226	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J64717	.	polyA_site	3227	3240	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J64717	.	stop_codon	3233	3233	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J64717	.	terminator	3112	3191	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J64714
BBa_J64717	.	engineered_region	3112	3191	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J64717	.	stem_loop	3123	3166	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J64717	.	CDS	1749	2357	.	+	0	ID=BBa_J64718;Name=BBa_J64718;Parent=BBa_J64714
>BBa_J64717
gcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaaca
tacagatttccggcgaaatcaaggctacctgccagttctggcaggtgtgaaatctggcagtttttggtacacgaaagct
actagagaaagaggagaaatactagatgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaa
atgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacg
gttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtat
cttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccg
cacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaag
ctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaa
aatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctg
cgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttcctaat
aatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgt
atggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccg
ctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaa
agcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgtt
atgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagtta
aactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacg
tatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgct
gaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatca
cctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacg
ctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttc
gttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttta
tatactagagtcctgtgaaatctggcagttacctgccagttctggcaggtctaaaaagtgttctactagagaaagagga
gaaatactagatgatcgaactgctgtccgaatccctggaaggtctgtccgctgctatgatcgctgaactgggtcgttac
cgtcaccaggttttcatcgaaaaactgggttgggacgttgtttccacctcccgtgttcgtgaccaggagttcgaccagt
tcgaccacccgcagacccgttacatcgttgctatgtcccgtcagggtatctgcggttgcgctcgtctgctgccgaccac
cgacgcttacctgctgaaagacgttttcgcttacctgtgctccgaaaccccgccgtccgacccgtccgtttgggaactg
tcccgttacgctgcttccgctgctgacgacccgcagctggctatgaaaatcttctggtcctccctccagtgcgcttggt
acctgggtgcttcctccgttgttgctgttaccaccaccgctatggaacgttacttcgttcgtaacggtgttatcctcca
gcgtctgggtccgccgcagaaagttaaaggtgaaaccctggttgctatctccttcccggcttaccaggaacgtggtctg
gaaatgctgctgcgttaccacccggaatggctccagggtgttccgctgtccatggctgtttaataatactagagaaaga
ggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgtt
aatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgca
ctactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagataccc
agatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaa
gatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggta
ttgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggc
agacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagac
cattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccc
tttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcat
ggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcg
ttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttat
atactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaat
actagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaa
gatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttc
atcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtc
actgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgagga
agcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctc
agcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggact
acgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaagga
agtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaac
ttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtc
ttattactctctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttta
tctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatact
agagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactag
atgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcg
tgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattccctt
cacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggcc
gtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccgga
tgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcag
cgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatg
atcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcg
aacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcac
ggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcg
ctgggtctcatctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttt
atctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata