BBa_J64717
type: ComponentDefinition
persistent identity: BBa J64717
display id: BBa_J64717
version: 1
was generated by: iGEM to SBOL conversion
description: Self-assembled Pattern Formation Generator
created: 2007-03-25T12:00:00Z
modified: 2015-05-08T01:08:17Z
mutable provenance: edit
owned by: james
owned by: myers
mutable description: edit
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1431
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: project goal
star: false
creator: laura lavery
type: DnaRegion
role: partType/Project
role: SO:0000804
component: BBa_J64715
component: BBa_J64714
component: BBa_J64713
component: BBa_J64716
sequence annotation: BBa_J64713
sequence annotation: BBa_J64714
sequence annotation: BBa_J64715
sequence annotation: BBa_J64716
sequence: https://synbiohub.org/public/igem/BBa_J64717_sequence/1

BBa_J64715
type: Component
persistent identity: component2322984
display id: component2322984
version: 1
top level: BBa_J64717
definition: BBa_J64715
access: public

BBa_J64714
type: Component
persistent identity: component2322969
display id: component2322969
version: 1
top level: BBa_J64717
definition: BBa_J64714
access: public

BBa_J64713
type: Component
persistent identity: component2322951
display id: component2322951
version: 1
top level: BBa_J64717
definition: BBa_J64713
access: public

BBa_J64716
type: Component
persistent identity: component2322999
display id: component2322999
version: 1
top level: BBa_J64717
definition: BBa_J64716
access: public

BBa_J64713
type: SequenceAnnotation
persistent identity: annotation2322951
display id: annotation2322951
version: 1
top level: BBa_J64717
location: https://synbiohub.org/public/igem/BBa_J64717/annotation2322951/range2322951/1
component: BBa_J64713

https://synbiohub.org/public/igem/BBa_J64717/annotation2322951/range2322951/1
type: Range
persistent identity: range2322951
display id: range2322951
version: 1
top level: BBa_J64717
direction: direction/reverse
start: 1
end: 1661
orientation: inline

BBa_J64714
type: SequenceAnnotation
persistent identity: annotation2322969
display id: annotation2322969
version: 1
top level: BBa_J64717
location: https://synbiohub.org/public/igem/BBa_J64717/annotation2322969/range2322969/1
component: BBa_J64714

https://synbiohub.org/public/igem/BBa_J64717/annotation2322969/range2322969/1
type: Range
persistent identity: range2322969
display id: range2322969
version: 1
top level: BBa_J64717
direction: direction/reverse
start: 1670
end: 3240
orientation: inline

BBa_J64715
type: SequenceAnnotation
persistent identity: annotation2322984
display id: annotation2322984
version: 1
top level: BBa_J64717
location: https://synbiohub.org/public/igem/BBa_J64717/annotation2322984/range2322984/1
component: BBa_J64715

https://synbiohub.org/public/igem/BBa_J64717/annotation2322984/range2322984/1
type: Range
persistent identity: range2322984
display id: range2322984
version: 1
top level: BBa_J64717
direction: direction/reverse
start: 3249
end: 4183
orientation: inline

BBa_J64716
type: SequenceAnnotation
persistent identity: annotation2322999
display id: annotation2322999
version: 1
top level: BBa_J64717
location: https://synbiohub.org/public/igem/BBa_J64717/annotation2322999/range2322999/1
component: BBa_J64716

https://synbiohub.org/public/igem/BBa_J64717/annotation2322999/range2322999/1
type: Range
persistent identity: range2322999
display id: range2322999
version: 1
top level: BBa_J64717
direction: direction/reverse
start: 4192
end: 5132
orientation: inline

BBa_J64713
type: ComponentDefinition
persistent identity: BBa J64713
display id: BBa_J64713
version: 1
was generated by: iGEM to SBOL conversion
description: LasR/LasI Inducible (RHLR/RHLI repressible) positive feedback production of 3OC12 HSL
created: 2007-03-24T12:00:00Z
modified: 2015-05-08T01:08:17Z
mutable provenance: For source of the promoter see j64712. The rest of the parts existed in the registry already.
owned by: james
owned by: myers
mutable description: This construct will produce more 3OC12 HSL when the circuit is given an inital amount of 3OC12 and when LasR is present in the system. This construct will also produce RFP as a reporter when expressed.
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1431
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: project goals
star: false
creator: laura lavery
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0015
component: BBa_E1010
component: BBa_B0034
component: BBa_C0178
component: BBa_J64712
sequence annotation: BBa_J64712
sequence annotation: BBa_B0034
sequence annotation: BBa_C0178
sequence annotation: BBa_E1010
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_J64713_sequence/1

BBa_B0015
type: Component
persistent identity: component2265280
display id: component2265280
version: 1
top level: BBa_J64713
definition: BBa_B0015
access: public

BBa_E1010
type: Component
persistent identity: component2265273
display id: component2265273
version: 1
top level: BBa_J64713
definition: mRFP1
access: public

BBa_B0034
type: Component
persistent identity: component2265270
display id: component2265270
version: 1
top level: BBa_J64713
definition: BBa_B0034
access: public

BBa_C0178
type: Component
persistent identity: component2265268
display id: component2265268
version: 1
top level: BBa_J64713
definition: lasI
access: public

BBa_B0034
type: Component
persistent identity: component2265266
display id: component2265266
version: 1
top level: BBa_J64713
definition: BBa_B0034
access: public

BBa_J64712
type: Component
persistent identity: component2265264
display id: component2265264
version: 1
top level: BBa_J64713
definition: BBa_J64712
access: public

BBa_J64712
type: SequenceAnnotation
persistent identity: annotation2265264
display id: annotation2265264
version: 1
top level: BBa_J64713
location: https://synbiohub.org/public/igem/BBa_J64713/annotation2265264/range2265264/1
component: BBa_J64712

https://synbiohub.org/public/igem/BBa_J64713/annotation2265264/range2265264/1
type: Range
persistent identity: range2265264
display id: range2265264
version: 1
top level: BBa_J64713
direction: direction/reverse
start: 1
end: 157
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2265266
display id: annotation2265266
version: 1
top level: BBa_J64713
location: https://synbiohub.org/public/igem/BBa_J64713/annotation2265266/range2265266/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J64713/annotation2265266/range2265266/1
type: Range
persistent identity: range2265266
display id: range2265266
version: 1
top level: BBa_J64713
direction: direction/reverse
start: 166
end: 177
orientation: inline

BBa_C0178
type: SequenceAnnotation
persistent identity: annotation2265268
display id: annotation2265268
version: 1
top level: BBa_J64713
location: https://synbiohub.org/public/igem/BBa_J64713/annotation2265268/range2265268/1
component: BBa_C0178

https://synbiohub.org/public/igem/BBa_J64713/annotation2265268/range2265268/1
type: Range
persistent identity: range2265268
display id: range2265268
version: 1
top level: BBa_J64713
direction: direction/reverse
start: 184
end: 792
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2265270
display id: annotation2265270
version: 1
top level: BBa_J64713
location: https://synbiohub.org/public/igem/BBa_J64713/annotation2265270/range2265270/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J64713/annotation2265270/range2265270/1
type: Range
persistent identity: range2265270
display id: range2265270
version: 1
top level: BBa_J64713
direction: direction/reverse
start: 801
end: 812
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2265273
display id: annotation2265273
version: 1
top level: BBa_J64713
location: https://synbiohub.org/public/igem/BBa_J64713/annotation2265273/range2265273/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_J64713/annotation2265273/range2265273/1
type: Range
persistent identity: range2265273
display id: range2265273
version: 1
top level: BBa_J64713
direction: direction/reverse
start: 819
end: 1524
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2265280
display id: annotation2265280
version: 1
top level: BBa_J64713
location: https://synbiohub.org/public/igem/BBa_J64713/annotation2265280/range2265280/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_J64713/annotation2265280/range2265280/1
type: Range
persistent identity: range2265280
display id: range2265280
version: 1
top level: BBa_J64713
direction: direction/reverse
start: 1533
end: 1661
orientation: inline

BBa_J64714
type: ComponentDefinition
persistent identity: BBa J64714
display id: BBa_J64714
version: 1
was generated by: iGEM to SBOL conversion
description: RHLR/RHLI Inducible (LasR/LasI repressible) positive feedback production of C4 HSL
created: 2007-03-24T12:00:00Z
modified: 2015-05-08T01:08:17Z
mutable provenance: J64800 was designed using R0071 and the binding site for LasR/LasI was taked from R0079. The rest of the parts were already in the registry.
owned by: james
owned by: myers
mutable description: This construct will produce more C4 HSL when the circuit is given an inital amount of C4 HSL and when RHLR is present in the system. This construct will also produce GFP as a reporter when expressed.
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1431
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Project goals
star: false
creator: laura lavery
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0012
component: BBa_B0010
component: BBa_E0040
component: BBa_B0034
component: BBa_J64718
component: BBa_J64800
sequence annotation: BBa_J64800
sequence annotation: BBa_B0034
sequence annotation: BBa_J64718
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J64714_sequence/1

BBa_B0012
type: Component
persistent identity: component1921385
display id: component1921385
version: 1
top level: BBa_J64714
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1921383
display id: component1921383
version: 1
top level: BBa_J64714
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component1921382
display id: component1921382
version: 1
top level: BBa_J64714
definition: GFP
access: public

BBa_B0034
type: Component
persistent identity: component1921381
display id: component1921381
version: 1
top level: BBa_J64714
definition: BBa_B0034
access: public

BBa_J64718
type: Component
persistent identity: component1921379
display id: component1921379
version: 1
top level: BBa_J64714
definition: BBa_J64718
access: public

BBa_B0034
type: Component
persistent identity: component1921378
display id: component1921378
version: 1
top level: BBa_J64714
definition: BBa_B0034
access: public

BBa_J64800
type: Component
persistent identity: component1921376
display id: component1921376
version: 1
top level: BBa_J64714
definition: BBa_J64800
access: public

BBa_J64800
type: SequenceAnnotation
persistent identity: annotation1921376
display id: annotation1921376
version: 1
top level: BBa_J64714
location: https://synbiohub.org/public/igem/BBa_J64714/annotation1921376/range1921376/1
component: BBa_J64800

https://synbiohub.org/public/igem/BBa_J64714/annotation1921376/range1921376/1
type: Range
persistent identity: range1921376
display id: range1921376
version: 1
top level: BBa_J64714
direction: direction/reverse
start: 1
end: 53
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1921378
display id: annotation1921378
version: 1
top level: BBa_J64714
location: https://synbiohub.org/public/igem/BBa_J64714/annotation1921378/range1921378/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J64714/annotation1921378/range1921378/1
type: Range
persistent identity: range1921378
display id: range1921378
version: 1
top level: BBa_J64714
direction: direction/reverse
start: 62
end: 73
orientation: inline

BBa_J64718
type: SequenceAnnotation
persistent identity: annotation1921379
display id: annotation1921379
version: 1
top level: BBa_J64714
location: https://synbiohub.org/public/igem/BBa_J64714/annotation1921379/range1921379/1
component: BBa_J64718

https://synbiohub.org/public/igem/BBa_J64714/annotation1921379/range1921379/1
type: Range
persistent identity: range1921379
display id: range1921379
version: 1
top level: BBa_J64714
direction: direction/reverse
start: 80
end: 688
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1921381
display id: annotation1921381
version: 1
top level: BBa_J64714
location: https://synbiohub.org/public/igem/BBa_J64714/annotation1921381/range1921381/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J64714/annotation1921381/range1921381/1
type: Range
persistent identity: range1921381
display id: range1921381
version: 1
top level: BBa_J64714
direction: direction/reverse
start: 697
end: 708
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1921382
display id: annotation1921382
version: 1
top level: BBa_J64714
location: https://synbiohub.org/public/igem/BBa_J64714/annotation1921382/range1921382/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_J64714/annotation1921382/range1921382/1
type: Range
persistent identity: range1921382
display id: range1921382
version: 1
top level: BBa_J64714
direction: direction/reverse
start: 715
end: 1434
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1921383
display id: annotation1921383
version: 1
top level: BBa_J64714
location: https://synbiohub.org/public/igem/BBa_J64714/annotation1921383/range1921383/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J64714/annotation1921383/range1921383/1
type: Range
persistent identity: range1921383
display id: range1921383
version: 1
top level: BBa_J64714
direction: direction/forward
start: 1443
end: 1522
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1921385
display id: annotation1921385
version: 1
top level: BBa_J64714
location: https://synbiohub.org/public/igem/BBa_J64714/annotation1921385/range1921385/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J64714/annotation1921385/range1921385/1
type: Range
persistent identity: range1921385
display id: range1921385
version: 1
top level: BBa_J64714
direction: direction/forward
start: 1531
end: 1571
orientation: inline

BBa_J64715
type: ComponentDefinition
persistent identity: BBa J64715
display id: BBa_J64715
version: 1
was generated by: iGEM to SBOL conversion
description: Constituive expression of LasR
created: 2007-03-25T12:00:00Z
modified: 2015-05-08T01:08:17Z
mutable provenance: Registry parts
owned by: james
owned by: myers
mutable description: In the absence of CI this strong promoter expresses LasR.
discontinued: false
dominant: false
group u list: _98_
m user id: 0
owner id: 1431
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: project goal
star: false
creator: laura lavery
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0012
component: BBa_B0010
component: BBa_C0179
component: BBa_B0034
component: BBa_R0051
sequence annotation: BBa_R0051
sequence annotation: BBa_B0034
sequence annotation: BBa_C0179
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J64715_sequence/1

BBa_B0012
type: Component
persistent identity: component1921464
display id: component1921464
version: 1
top level: BBa_J64715
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1921462
display id: component1921462
version: 1
top level: BBa_J64715
definition: BBa_B0010
access: public

BBa_C0179
type: Component
persistent identity: component1921461
display id: component1921461
version: 1
top level: BBa_J64715
definition: lasR
access: public

BBa_B0034
type: Component
persistent identity: component1921460
display id: component1921460
version: 1
top level: BBa_J64715
definition: BBa_B0034
access: public

BBa_R0051
type: Component
persistent identity: component1921455
display id: component1921455
version: 1
top level: BBa_J64715
definition: cI lam
access: public

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation1921455
display id: annotation1921455
version: 1
top level: BBa_J64715
location: https://synbiohub.org/public/igem/BBa_J64715/annotation1921455/range1921455/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_J64715/annotation1921455/range1921455/1
type: Range
persistent identity: range1921455
display id: range1921455
version: 1
top level: BBa_J64715
direction: direction/forward
start: 1
end: 49
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1921460
display id: annotation1921460
version: 1
top level: BBa_J64715
location: https://synbiohub.org/public/igem/BBa_J64715/annotation1921460/range1921460/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J64715/annotation1921460/range1921460/1
type: Range
persistent identity: range1921460
display id: range1921460
version: 1
top level: BBa_J64715
direction: direction/reverse
start: 58
end: 69
orientation: inline

BBa_C0179
type: SequenceAnnotation
persistent identity: annotation1921461
display id: annotation1921461
version: 1
top level: BBa_J64715
location: https://synbiohub.org/public/igem/BBa_J64715/annotation1921461/range1921461/1
component: BBa_C0179

https://synbiohub.org/public/igem/BBa_J64715/annotation1921461/range1921461/1
type: Range
persistent identity: range1921461
display id: range1921461
version: 1
top level: BBa_J64715
direction: direction/reverse
start: 76
end: 798
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1921462
display id: annotation1921462
version: 1
top level: BBa_J64715
location: https://synbiohub.org/public/igem/BBa_J64715/annotation1921462/range1921462/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J64715/annotation1921462/range1921462/1
type: Range
persistent identity: range1921462
display id: range1921462
version: 1
top level: BBa_J64715
direction: direction/forward
start: 807
end: 886
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1921464
display id: annotation1921464
version: 1
top level: BBa_J64715
location: https://synbiohub.org/public/igem/BBa_J64715/annotation1921464/range1921464/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J64715/annotation1921464/range1921464/1
type: Range
persistent identity: range1921464
display id: range1921464
version: 1
top level: BBa_J64715
direction: direction/forward
start: 895
end: 935
orientation: inline

BBa_J64716
type: ComponentDefinition
persistent identity: BBa J64716
display id: BBa_J64716
version: 1
was generated by: iGEM to SBOL conversion
description: Constituive expression of RHLR
created: 2007-03-25T12:00:00Z
modified: 2015-05-08T01:08:17Z
mutable provenance: Registry
owned by: james
owned by: myers
mutable description: In the absence of CI this strong promoter expresses RHLR.
discontinued: false
dominant: false
group u list: _98_
m user id: 0
owner id: 1431
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Project goals
star: false
creator: laura lavery
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0012
component: BBa_B0010
component: BBa_C0171
component: BBa_B0034
component: BBa_R0051
sequence annotation: BBa_R0051
sequence annotation: BBa_B0034
sequence annotation: BBa_C0171
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J64716_sequence/1

BBa_B0012
type: Component
persistent identity: component1921130
display id: component1921130
version: 1
top level: BBa_J64716
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1921128
display id: component1921128
version: 1
top level: BBa_J64716
definition: BBa_B0010
access: public

BBa_C0171
type: Component
persistent identity: component1921127
display id: component1921127
version: 1
top level: BBa_J64716
definition: rhIR
access: public

BBa_B0034
type: Component
persistent identity: component1921126
display id: component1921126
version: 1
top level: BBa_J64716
definition: BBa_B0034
access: public

BBa_R0051
type: Component
persistent identity: component1921121
display id: component1921121
version: 1
top level: BBa_J64716
definition: cI lam
access: public

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation1921121
display id: annotation1921121
version: 1
top level: BBa_J64716
location: https://synbiohub.org/public/igem/BBa_J64716/annotation1921121/range1921121/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_J64716/annotation1921121/range1921121/1
type: Range
persistent identity: range1921121
display id: range1921121
version: 1
top level: BBa_J64716
direction: direction/forward
start: 1
end: 49
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1921126
display id: annotation1921126
version: 1
top level: BBa_J64716
location: https://synbiohub.org/public/igem/BBa_J64716/annotation1921126/range1921126/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J64716/annotation1921126/range1921126/1
type: Range
persistent identity: range1921126
display id: range1921126
version: 1
top level: BBa_J64716
direction: direction/reverse
start: 58
end: 69
orientation: inline

BBa_C0171
type: SequenceAnnotation
persistent identity: annotation1921127
display id: annotation1921127
version: 1
top level: BBa_J64716
location: https://synbiohub.org/public/igem/BBa_J64716/annotation1921127/range1921127/1
component: BBa_C0171

https://synbiohub.org/public/igem/BBa_J64716/annotation1921127/range1921127/1
type: Range
persistent identity: range1921127
display id: range1921127
version: 1
top level: BBa_J64716
direction: direction/reverse
start: 76
end: 804
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1921128
display id: annotation1921128
version: 1
top level: BBa_J64716
location: https://synbiohub.org/public/igem/BBa_J64716/annotation1921128/range1921128/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J64716/annotation1921128/range1921128/1
type: Range
persistent identity: range1921128
display id: range1921128
version: 1
top level: BBa_J64716
direction: direction/forward
start: 813
end: 892
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1921130
display id: annotation1921130
version: 1
top level: BBa_J64716
location: https://synbiohub.org/public/igem/BBa_J64716/annotation1921130/range1921130/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J64716/annotation1921130/range1921130/1
type: Range
persistent identity: range1921130
display id: range1921130
version: 1
top level: BBa_J64716
direction: direction/forward
start: 901
end: 941
orientation: inline

BBa_J64712
type: ComponentDefinition
persistent identity: BBa J64712
display id: BBa_J64712
version: 1
was generated by: iGEM to SBOL conversion
description: LasR/LasI Inducible & RHLR/RHLI repressible Promoter
created: 2007-03-24T12:00:00Z
modified: 2015-05-08T01:08:17Z
mutable provenance: Rust L. et. al., "Analysis of the Pseudomonas aeruginosa Elastase (lasB) Regulatory Region". Journal of Bacteriology, 1996.
owned by: james
owned by: myers
mutable description: This part is similar to BBa_R0079 but has an added binding site for RHLR/RHLI in between the -35 and -10 region. Thus this promoter is induced by LasR/LasI but repressed by RHLR/RHLI binding.
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1431
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The natural spacer between the -35 and -10 is 14bp and the natural O1 site for RHLR/RHLI is 20bp long. There was not much over lap of the -35 or the -10 with this sequence, so it was nessicary to chop off some of the binding site sequence in order to maintain the correct spacing and preserve the -35 and -10 sequence. The binding site sequence is missing a tcc on is 5' end and a t on its 3' end.
star: false
creator: laura lavery
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LasR/LasI O2
sequence annotation: LasR/LasI O1
sequence annotation: -35
sequence annotation: RHLR/RHLI
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_J64712_sequence/1

LasR/LasI O2
type: SequenceAnnotation
persistent identity: annotation1921064
display id: annotation1921064
version: 1
top level: BBa_J64712
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921064/range1921064/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_J64712/annotation1921064/range1921064/1
type: Range
persistent identity: range1921064
display id: range1921064
version: 1
top level: BBa_J64712
direction: direction/forward
start: 46
end: 64
orientation: inline

LasR/LasI O1
type: SequenceAnnotation
persistent identity: annotation1921065
display id: annotation1921065
version: 1
top level: BBa_J64712
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921065/range1921065/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_J64712/annotation1921065/range1921065/1
type: Range
persistent identity: range1921065
display id: range1921065
version: 1
top level: BBa_J64712
direction: direction/forward
start: 106
end: 125
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1921067
display id: annotation1921067
version: 1
top level: BBa_J64712
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921067/range1921067/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J64712/annotation1921067/range1921067/1
type: Range
persistent identity: range1921067
display id: range1921067
version: 1
top level: BBa_J64712
direction: direction/forward
start: 117
end: 122
orientation: inline

RHLR/RHLI
type: SequenceAnnotation
persistent identity: annotation1921066
display id: annotation1921066
version: 1
top level: BBa_J64712
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921066/range1921066/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_J64712/annotation1921066/range1921066/1
type: Range
persistent identity: range1921066
display id: range1921066
version: 1
top level: BBa_J64712
direction: direction/forward
start: 125
end: 141
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1921068
display id: annotation1921068
version: 1
top level: BBa_J64712
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921068/range1921068/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J64712/annotation1921068/range1921068/1
type: Range
persistent identity: range1921068
display id: range1921068
version: 1
top level: BBa_J64712
direction: direction/forward
start: 140
end: 145
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

lasI
type: ComponentDefinition
persistent identity: BBa C0178
display id: BBa_C0178
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer synthetase for PAI from Pseudomonas aeruginosa (no LVA)
created: 2004-05-26T11:00:00Z
modified: 2015-08-31T04:07:24Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: same as C0078 except no LVA tag
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: lasI
sequence: https://synbiohub.org/public/igem/BBa_C0178_sequence/1

lasI
type: SequenceAnnotation
persistent identity: annotation1937124
display id: annotation1937124
version: 1
top level: lasI
location: https://synbiohub.org/public/igem/BBa_C0178/annotation1937124/range1937124/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0178/annotation1937124/range1937124/1
type: Range
persistent identity: range1937124
display id: range1937124
version: 1
top level: lasI
direction: direction/forward
start: 1
end: 609
orientation: inline

BBa_J64718
type: ComponentDefinition
persistent identity: BBa J64718
display id: BBa_J64718
version: 1
was generated by: iGEM to SBOL conversion
description: RHLI (no LVA)
created: 2007-03-25T11:00:00Z
modified: 2015-05-08T01:08:17Z
mutable provenance: C0070
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is C0070 without the LVA tag
discontinued: false
dominant: false
group u list: _98_
m user id: 0
owner id: 1431
owning group id: 98
sample status: In stock
status: status/Available
bookmark: false
mutable notes: project goals
star: false
creator: laura lavery
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_J64718_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa R0051
display id: BBa_R0051
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lambda cI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Incompatible with host expressing cI repressor.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0051
sequence annotation: OR2
sequence annotation: -35
sequence annotation: OR1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0051_sequence/1

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation7067
display id: annotation7067
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
type: Range
persistent identity: range7067
display id: range7067
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 49
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2025
display id: annotation2025
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
type: Range
persistent identity: range2025
display id: range2025
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 17
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2023
display id: annotation2023
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
type: Range
persistent identity: range2023
display id: range2023
version: 1
top level: cI lam
direction: direction/forward
start: 15
end: 20
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2024
display id: annotation2024
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
type: Range
persistent identity: range2024
display id: range2024
version: 1
top level: cI lam
direction: direction/forward
start: 25
end: 41
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2022
display id: annotation2022
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
type: Range
persistent identity: range2022
display id: range2022
version: 1
top level: cI lam
direction: direction/forward
start: 38
end: 43
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_J64800
type: ComponentDefinition
persistent identity: BBa J64800
display id: BBa_J64800
version: 1
was generated by: iGEM to SBOL conversion
description: RHLR/RHLI Inducible & LasR/LasI repressible Promoter
created: 2007-03-24T12:00:00Z
modified: 2015-05-08T01:08:17Z
mutable provenance: Rust L. et. al., "Analysis of the Pseudomonas aeruginosa Elastase (lasB) Regulatory Region". Journal of Bacteriology, 1996.
owned by: james
owned by: myers
mutable description: This part is similar to BBa_R0079 but has an added binding site for RHLR/RHLI in between the -35 and -10 region. Thus this promoter is induced by LasR/LasI but repressed by RHLR/RHLi binding.
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1431
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: There is a 17bp spacer between the -35 and -10 regions and the binding site for RHLR/RHLI is 20bp long. I was able to fit the whole binding site in the spacer region because the last three base pairs of the -35 region and the first three base pairs of the RHLR/RHLI binding site are the same.
star: false
creator: laura lavery
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: RHLR/RHLI
sequence annotation: -35
sequence annotation: LasR/LasI
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_J64800_sequence/1

RHLR/RHLI
type: SequenceAnnotation
persistent identity: annotation1921060
display id: annotation1921060
version: 1
top level: BBa_J64800
location: https://synbiohub.org/public/igem/BBa_J64800/annotation1921060/range1921060/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_J64800/annotation1921060/range1921060/1
type: Range
persistent identity: range1921060
display id: range1921060
version: 1
top level: BBa_J64800
direction: direction/forward
start: 1
end: 23
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1921062
display id: annotation1921062
version: 1
top level: BBa_J64800
location: https://synbiohub.org/public/igem/BBa_J64800/annotation1921062/range1921062/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J64800/annotation1921062/range1921062/1
type: Range
persistent identity: range1921062
display id: range1921062
version: 1
top level: BBa_J64800
direction: direction/forward
start: 19
end: 24
orientation: inline

LasR/LasI
type: SequenceAnnotation
persistent identity: annotation1921061
display id: annotation1921061
version: 1
top level: BBa_J64800
location: https://synbiohub.org/public/igem/BBa_J64800/annotation1921061/range1921061/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_J64800/annotation1921061/range1921061/1
type: Range
persistent identity: range1921061
display id: range1921061
version: 1
top level: BBa_J64800
direction: direction/forward
start: 23
end: 40
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1921063
display id: annotation1921063
version: 1
top level: BBa_J64800
location: https://synbiohub.org/public/igem/BBa_J64800/annotation1921063/range1921063/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J64800/annotation1921063/range1921063/1
type: Range
persistent identity: range1921063
display id: range1921063
version: 1
top level: BBa_J64800
direction: direction/forward
start: 42
end: 47
orientation: inline

lasR
type: ComponentDefinition
persistent identity: BBa C0179
display id: BBa_C0179
version: 1
was generated by: iGEM to SBOL conversion
description: lasR activator from P. aeruginosa PAO1(no LVA)
created: 2004-05-26T11:00:00Z
modified: 2015-08-31T04:07:24Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: same as C0079 except no LVA tag
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_C0179_sequence/1

rhIR
type: ComponentDefinition
persistent identity: BBa C0171
display id: BBa_C0171
version: 1
was generated by: iGEM to SBOL conversion
description: rhlR repressor/activator from P. aeruginosa PA3477 (no LVA)
created: 2004-05-26T11:00:00Z
modified: 2015-08-31T04:07:24Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: same as C0071 except no LVA tag
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_C0171_sequence/1

https://synbiohub.org/public/igem/BBa_J64717_sequence/1
type: Sequence
persistent identity: BBa J64717 sequence
display id: BBa_J64717_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaaggctacctgccagttctggcaggtgtgaaatctggcagtttttggtacacgaaagctactagagaaagaggagaaatactagatgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttcctaataatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcctgtgaaatctggcagttacctgccagttctggcaggtctaaaaagtgttctactagagaaagaggagaaatactagatgatcgaactgctgtccgaatccctggaaggtctgtccgctgctatgatcgctgaactgggtcgttaccgtcaccaggttttcatcgaaaaactgggttgggacgttgtttccacctcccgtgttcgtgaccaggagttcgaccagttcgaccacccgcagacccgttacatcgttgctatgtcccgtcagggtatctgcggttgcgctcgtctgctgccgaccaccgacgcttacctgctgaaagacgtt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J64713_sequence/1
type: Sequence
persistent identity: BBa J64713 sequence
display id: BBa_J64713_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaaggctacctgccagttctggcaggtgtgaaatctggcagtttttggtacacgaaagctactagagaaagaggagaaatactagatgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttcctaataatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J64714_sequence/1
type: Sequence
persistent identity: BBa J64714 sequence
display id: BBa_J64714_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcctgtgaaatctggcagttacctgccagttctggcaggtctaaaaagtgttctactagagaaagaggagaaatactagatgatcgaactgctgtccgaatccctggaaggtctgtccgctgctatgatcgctgaactgggtcgttaccgtcaccaggttttcatcgaaaaactgggttgggacgttgtttccacctcccgtgttcgtgaccaggagttcgaccagttcgaccacccgcagacccgttacatcgttgctatgtcccgtcagggtatctgcggttgcgctcgtctgctgccgaccaccgacgcttacctgctgaaagacgttttcgcttacctgtgctccgaaaccccgccgtccgacccgtccgtttgggaactgtcccgttacgctgcttccgctgctgacgacccgcagctggctatgaaaatcttctggtcctccctccagtgcgcttggtacctgggtgcttcctccgttgttgctgttaccaccaccgctatggaacgttacttcgttcgtaacggtgttatcctccagcgtctgggtccgccgcagaaagttaaaggtgaaaccctggttgctatctccttcccggcttaccaggaacgtggtctggaaatgctgctgcgttaccacccggaatggctccagggtgttccgctgtccatggctgtttaataatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J64715_sequence/1
type: Sequence
persistent identity: BBa J64715 sequence
display id: BBa_J64715_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J64716_sequence/1
type: Sequence
persistent identity: BBa J64716 sequence
display id: BBa_J64716_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J64712_sequence/1
type: Sequence
persistent identity: BBa J64712 sequence
display id: BBa_J64712_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaaggctacctgccagttctggcaggtgtgaaatctggcagtttttggtacacgaaagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J64718_sequence/1
type: Sequence
persistent identity: BBa J64718 sequence
display id: BBa_J64718_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatcgaactgctgtccgaatccctggaaggtctgtccgctgctatgatcgctgaactgggtcgttaccgtcaccaggttttcatcgaaaaactgggttgggacgttgtttccacctcccgtgttcgtgaccaggagttcgaccagttcgaccacccgcagacccgttacatcgttgctatgtcccgtcagggtatctgcggttgcgctcgtctgctgccgaccaccgacgcttacctgctgaaagacgttttcgcttacctgtgctccgaaaccccgccgtccgacccgtccgtttgggaactgtcccgttacgctgcttccgctgctgacgacccgcagctggctatgaaaatcttctggtcctccctccagtgcgcttggtacctgggtgcttcctccgttgttgctgttaccaccaccgctatggaacgttacttcgttcgtaacggtgttatcctccagcgtctgggtccgccgcagaaagttaaaggtgaaaccctggttgctatctccttcccggcttaccaggaacgtggtctggaaatgctgctgcgttaccacccggaatggctccagggtgttccgctgtccatggctgtttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0178_sequence/1
type: Sequence
persistent identity: BBa C0178 sequence
display id: BBa_C0178_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttcctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0051_sequence/1
type: Sequence
persistent identity: BBa R0051 sequence
display id: BBa_R0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0171_sequence/1
type: Sequence
persistent identity: BBa C0171 sequence
display id: BBa_C0171_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J64800_sequence/1
type: Sequence
persistent identity: BBa J64800 sequence
display id: BBa_J64800_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcctgtgaaatctggcagttacctgccagttctggcaggtctaaaaagtgttc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0179_sequence/1
type: Sequence
persistent identity: BBa C0179 sequence
display id: BBa_C0179_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctctaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z