LOCUS BBa_J64903 4383 bp DNA linear 22-Nov-2024 DEFINITION Light induced production of RNAi for glnG ACCESSION BBa_J64903 VERSION BBa_J64903.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23100 RBS 44..58 /label=RBS-1\Strong RBS 51..55 /label=RBS misc_feature 44..58 /label=BBa_B0030 RBS 44..58 /label=BBa_B0030 misc_feature 791..815 /label=Help:Barcodes CDS 65..815 /label=BBa_I15008 RBS 824..838 /label=RBS-1\Strong RBS 831..835 /label=RBS misc_feature 824..838 /label=BBa_B0030 RBS 824..838 /label=BBa_B0030 CDS 845..1594 /label=PcyA misc_feature 1595..1619 /label=Help:Barcodes CDS 845..1619 /label=BBa_I15009 RBS 1628..1642 /label=RBS-1\Strong RBS 1635..1639 /label=RBS misc_feature 1628..1642 /label=BBa_B0030 RBS 1628..1642 /label=BBa_B0030 CDS 1649..3886 /label=Cph8 misc_feature 3887..3911 /label=Help:Barcodes CDS 1649..3911 /label=BBa_I15010 stem_loop 3931..3974 /label=stem_loop misc_feature 3920..3999 /label=BBa_B0010 terminator 3920..3999 /label=BBa_B0010 stem_loop 4015..4034 /label=T7 TE misc_feature 4041..4041 /label=stop polyA_site 4035..4048 /label=polya misc_feature 4008..4048 /label=BBa_B0012 terminator 4008..4048 /label=BBa_B0012 misc_feature 4069..4086 /label=C1 OmpR misc_feature 4090..4107 /label=C2 OmpR misc_feature 4110..4127 /label=C3 OmpR promoter 4131..4136 /label=-35 promoter 4154..4159 /label=-10 promoter 4057..4164 /label=BBa_R0082 misc_feature 4173..4246 /label=BBa_J64975 stem_loop 4266..4309 /label=stem_loop misc_feature 4255..4334 /label=BBa_B0010 terminator 4255..4334 /label=BBa_B0010 stem_loop 4350..4369 /label=T7 TE misc_feature 4376..4376 /label=stop polyA_site 4370..4383 /label=polya misc_feature 4343..4383 /label=BBa_B0012 terminator 4343..4383 /label=BBa_B0012 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagattaaag aggagaaata 61 ctagatgagt gtcaacttag cttcccagtt gcgggaaggg acgaaaaaat cccactccat 121 ggcggagaac gtcggctttg tcaaatgctt cctcaagggc gttgtcgaga aaaattccta 181 ccgtaagctg gttggcaatc tctactttgt ctacagtgcc atggaagagg aaatggcaaa 241 atttaaggac catcccatcc tcagccacat ttacttcccc gaactcaacc gcaaacaaag 301 cctagagcaa gacctgcaat tctattacgg ctccaactgg cggcaagaag tgaaaatttc 361 tgccgctggc caagcctatg tggaccgagt ccggcaagtg gccgctacgg cccctgaatt 421 gttggtggcc cattcctaca cccgttacct gggggatctt tccggcggtc aaattctcaa 481 gaaaattgcc caaaatgcca tgaatctcca cgatggtggc acagctttct atgaatttgc 541 cgacattgat gacgaaaagg cttttaaaaa tacctaccgt caagctatga atgatctgcc 601 cattgaccaa gccaccgccg aacggattgt ggatgaagcc aatgacgcct ttgccatgaa 661 catgaaaatg ttcaacgaac ttgaaggcaa cctgatcaag gcgatcggca ttatggtgtt 721 caacagcctc acccgtcgcc gcagtcaagg cagcaccgaa gttggcctcg ccacctccga 781 aggctaataa cgctgatagt gctagtgtag atcgctacta gagattaaag aggagaaata 841 ctagatggcc gtcactgatt taagtttgac caattcttcc ctgatgccta cgttgaaccc 901 gatgattcaa cagttggccc tggcgatcgc cgctagttgg caaagtttac ccctcaagcc 961 ctatcaattg ccggaggatt tgggctacgt agaaggccgc ctggaagggg aaaagttagt 1021 gattgaaaat cggtgctacc aaacgcccca gtttcgcaaa atgcatttgg agttggccaa 1081 ggtgggcaaa gggttggata ttctccactg tgtaatgttt cctgagcctt tatacggtct 1141 acctttgttt ggctgtgaca ttgtggccgg ccccggtgga gtaagtgcgg ctattgcgga 1201 tctatccccc acccaaagcg atcgccaatt gcccgcagcg taccaaaaat cattggcaga 1261 gctaggccag ccagaatttg agcaacaacg ggaattgccc ccctggggag aaatattttc 1321 tgaatattgt ttattcatcc gtcccagcaa tgtcactgaa gaagaaagat ttgtacaaag 1381 ggtagtggac tttttgcaaa ttcattgtca ccaatccatc gttgccgaac ccttgtctga 1441 agctcaaact ttggagcacc gtcaggggca aattcattac tgccaacaac aacagaaaaa 1501 tgataaaacc cgtcgggtac tggaaaaagc ttttggggaa gcttgggcgg aacggtatat 1561 gagccaagtc ttatttgatg ttatccaata ataactctga tagtgctagt gtagatctct 1621 actagagatt aaagaggaga aatactagat ggccaccacc gtacaactca gcgaccaatc 1681 cctccgtcag ctagaaaccc tcgccatcca caccgcccac ctgattcagc cccacggttt 1741 agtggtggtc ctgcaagaac cagacctcac catcagccaa attagcgcca actgcaccgg 1801 cattttaggg cgatcgccag aggatttgtt gggcagaacc ctaggggaag tgtttgatag 1861 ctttcagatt gatcccatcc agagtcgcct aacggccgga caaatcagca gcctcaaccc 1921 cagtaaactt tgggcgcggg tcatggggga cgactttgtc atttttgacg gggtttttca 1981 tcgcaacagt gacggtttat tggtatgtga actcgagcca gcctacactt ccgataatct 2041 gcccttcctc ggtttttatc acatggccaa cgctgccctg aatcggttgc gccaacaagc 2101 taatctacgg gatttctacg atgttattgt cgaagaagtc cgccgtatga ctggctttga 2161 ccgggtgatg ctataccgct ttgatgaaaa taaccacggt gatgtcattg ccgaagataa 2221 acgggatgat atggaaccct atttgggcct gcactatccc gaatcggata ttccccaacc 2281 cgcccgtcgg ctatttatcc acaaccccat tcgagtaatt cccgatgttt atggtgtggc 2341 ggtgcccctg accccagcgg ttaaccccag caccaaccga gcggtggatt taacagaatc 2401 cattctgcgc agtgcgtacc attgccactt gacctatctg aaaaatatgg gggtaggagc 2461 gtctttaacc atttccctaa ttaaggacgg ccatctctgg gggctcattg cctgccacca 2521 tcaaaccccc aaagtaattc cctttgaact gcgtaaagcc tgcgaatttt ttggtcgggt 2581 ggtgtttagc aacatttccg cccaggaaga tacggaaacc ttcgattacc gggtgcagct 2641 ggcggagcat gaagcggttt tattggacaa aatgaccacg gcggcggatt ttgtcgaagg 2701 attaactaat catcccgatc gcctgttggg attaacgggc tcccaggggg cggccatttg 2761 ctttggggaa aaattgattt tagtagggga aaccccggac gagaaagcag tgcaatattt 2821 actgcaatgg ttggagaatc gggaagtgca agacgttttc ttcacctctt ccctctcaca 2881 aatttatcct gatgcagtga attttaaatc cgtggccagt ggcttattgg ccattcccat 2941 tgcccgtcac aactttttgc tctggtttcg ccctgaagtg ttgcaaacgg ttaattgggg 3001 cggtgaccca aatcatgctt acgaagctac ccaggaagac ggtaaaatcg agctccatcc 3061 ccgccaatcc tttgacctct ggaaagaaat tgtccgactc caatctttgc cctggcaatc 3121 ggtggaaatc caaagtgccc tggccctgaa aaaggcgatc gtcaacctca ttttgcgcca 3181 ggcagaagaa ttgcatatgg cggctggtgt taagcaactg gcggatgacc gcacgctgct 3241 gatggcgggg gtaagtcacg acttgcgcac gccgctgacg cgtattcgcc tggcgactga 3301 gatgatgagc gagcaggatg gctatctggc agaatcgatc aataaagata tcgaagagtg 3361 caacgccatc attgagcagt ttatcgacta cctgcgcacc gggcaggaga tgccgatgga 3421 aatggcggat cttaatgcag tactcggtga ggtgattgct gccgaaagtg gctatgagcg 3481 ggaaattgaa accgcgcttt accccggcag cattgaagtg aaaatgcacc cgctgtcgat 3541 caaacgcgcg gtggcgaata tggtggtcaa cgccgcccgt tatggcaatg gctgggtcaa 3601 agtcagcagc ggaacggagc cgaatcgcgc ctggttccag gtggaagatg acggtccggg 3661 aattgcgccg gaacaacgta agcacctgtt ccagccgttt gtccgcggcg acagtgcgcg 3721 caccattagc ggcacgggat tagggctggc aattgtgcag cgtatcgtgg ataaccataa 3781 cgggatgctg gagcttggca ccagcgagcg gggcgggctt tccattcgcg cctggctgcc 3841 agtgccggta acgcgggcgc agggcatgac aaaagaaggg taataactct gatagtgcta 3901 gtgtagatct ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 3961 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 4021 ccttcgggtg ggcctttctg cgtttatata ctagagtccc ttgcatttac attttgaaac 4081 atctatagcg ataaatgaaa catcttaaaa gttttagtat catattcgtg ttggattatt 4141 ctgcattttt ggggagaatg gacttactag agttactgac gaccgcgttg cgacatacgc 4201 aggggcataa acaggaagcg gcgcggctac tcggctcccc ggcgcctact agagccaggc 4261 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 4321 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 4381 ata //