##gff-version 3
##sequence-region BBa_J64903 1 4383
BBa_J64903	.	ribosome_entry_site	44	58	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J64903	.	ribosome_entry_site	51	55	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J64903	.	engineered_region	44	58	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J64903	.	ribosome_entry_site	44	58	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J64903	.	CDS	1649	3911	.	+	0	ID=BBa_I15010;Name=BBa_I15010
BBa_J64903	.	engineered_tag	3887	3911	.	+	0	ID=annotation2214031;Name=Help:Barcodes;Parent=BBa_I15010
BBa_J64903	.	CDS	1649	3886	.	+	0	ID=annotation1891613;Name=Cph8;Parent=BBa_I15010
BBa_J64903	.	mature_transcript_region	4173	4246	.	+	0	ID=BBa_J64975;Name=BBa_J64975
BBa_J64903	.	CDS	845	1619	.	+	0	ID=BBa_I15009;Name=BBa_I15009
BBa_J64903	.	CDS	845	1594	.	+	0	ID=annotation1891612;Name=PcyA;Parent=BBa_I15009
BBa_J64903	.	engineered_tag	1595	1619	.	+	0	ID=annotation2214032;Name=Help:Barcodes;Parent=BBa_I15009
BBa_J64903	.	CDS	65	815	.	+	0	ID=BBa_I15008;Name=BBa_I15008
BBa_J64903	.	engineered_tag	791	815	.	+	0	ID=annotation2214026;Name=Help:Barcodes;Parent=BBa_I15008
BBa_J64903	.	terminator	4008	4048	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J64903	.	stop_codon	4041	4041	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J64903	.	polyA_site	4035	4048	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J64903	.	stem_loop	4015	4034	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J64903	.	engineered_region	4008	4048	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J64903	.	terminator	3920	3999	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J64903	.	engineered_region	3920	3999	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J64903	.	stem_loop	3931	3974	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J64903	.	promoter	4057	4164	.	+	0	ID=BBa_R0082;Name=BBa_R0082
BBa_J64903	.	non_covalent_binding_site	4090	4107	.	+	0	ID=annotation301155;Name=C2 OmpR;Parent=BBa_R0082
BBa_J64903	.	non_covalent_binding_site	4110	4127	.	+	0	ID=annotation301156;Name=C3 OmpR;Parent=BBa_R0082
BBa_J64903	.	promoter	4154	4159	.	+	0	ID=annotation301167;Name=-10;Parent=BBa_R0082
BBa_J64903	.	promoter	4131	4136	.	+	0	ID=annotation301166;Name=-35;Parent=BBa_R0082
BBa_J64903	.	non_covalent_binding_site	4069	4086	.	+	0	ID=annotation301154;Name=C1 OmpR;Parent=BBa_R0082
BBa_J64903	.	terminator	4255	4334	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J64903	.	engineered_region	4255	4334	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J64903	.	stem_loop	4266	4309	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J64903	.	terminator	4343	4383	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J64903	.	stop_codon	4376	4376	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J64903	.	polyA_site	4370	4383	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J64903	.	stem_loop	4350	4369	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J64903	.	engineered_region	4343	4383	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J64903	.	ribosome_entry_site	1628	1642	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J64903	.	ribosome_entry_site	1635	1639	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J64903	.	engineered_region	1628	1642	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J64903	.	ribosome_entry_site	1628	1642	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J64903	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100
BBa_J64903	.	ribosome_entry_site	824	838	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J64903	.	ribosome_entry_site	831	835	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J64903	.	engineered_region	824	838	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J64903	.	ribosome_entry_site	824	838	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
>BBa_J64903
ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatgagtgtcaactta
gcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagg
gcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggc
aaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctg
caattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggc
aagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaat
tctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgac
gaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtgg
atgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcat
tatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctaataa
cgctgatagtgctagtgtagatcgctactagagattaaagaggagaaatactagatggccgtcactgatttaagtttga
ccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagttt
acccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaa
aatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctcc
actgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaag
tgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagcta
ggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtc
ccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgt
tgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaat
gataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatg
ttatccaataataactctgatagtgctagtgtagatctctactagagattaaagaggagaaatactagatggccaccac
cgtacaactcagcgaccaatccctccgtcagctagaaaccctcgccatccacaccgcccacctgattcagccccacggt
ttagtggtggtcctgcaagaaccagacctcaccatcagccaaattagcgccaactgcaccggcattttagggcgatcgc
cagaggatttgttgggcagaaccctaggggaagtgtttgatagctttcagattgatcccatccagagtcgcctaacggc
cggacaaatcagcagcctcaaccccagtaaactttgggcgcgggtcatgggggacgactttgtcatttttgacggggtt
tttcatcgcaacagtgacggtttattggtatgtgaactcgagccagcctacacttccgataatctgcccttcctcggtt
tttatcacatggccaacgctgccctgaatcggttgcgccaacaagctaatctacgggatttctacgatgttattgtcga
agaagtccgccgtatgactggctttgaccgggtgatgctataccgctttgatgaaaataaccacggtgatgtcattgcc
gaagataaacgggatgatatggaaccctatttgggcctgcactatcccgaatcggatattccccaacccgcccgtcggc
tatttatccacaaccccattcgagtaattcccgatgtttatggtgtggcggtgcccctgaccccagcggttaaccccag
caccaaccgagcggtggatttaacagaatccattctgcgcagtgcgtaccattgccacttgacctatctgaaaaatatg
ggggtaggagcgtctttaaccatttccctaattaaggacggccatctctgggggctcattgcctgccaccatcaaaccc
ccaaagtaattccctttgaactgcgtaaagcctgcgaattttttggtcgggtggtgtttagcaacatttccgcccagga
agatacggaaaccttcgattaccgggtgcagctggcggagcatgaagcggttttattggacaaaatgaccacggcggcg
gattttgtcgaaggattaactaatcatcccgatcgcctgttgggattaacgggctcccagggggcggccatttgctttg
gggaaaaattgattttagtaggggaaaccccggacgagaaagcagtgcaatatttactgcaatggttggagaatcggga
agtgcaagacgttttcttcacctcttccctctcacaaatttatcctgatgcagtgaattttaaatccgtggccagtggc
ttattggccattcccattgcccgtcacaactttttgctctggtttcgccctgaagtgttgcaaacggttaattggggcg
gtgacccaaatcatgcttacgaagctacccaggaagacggtaaaatcgagctccatccccgccaatcctttgacctctg
gaaagaaattgtccgactccaatctttgccctggcaatcggtggaaatccaaagtgccctggccctgaaaaaggcgatc
gtcaacctcattttgcgccaggcagaagaattgcatatggcggctggtgttaagcaactggcggatgaccgcacgctgc
tgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcagga
tggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgc
accgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatg
agcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggc
gaatatggtggtcaacgccgcccgttatggcaatggctgggtcaaagtcagcagcggaacggagccgaatcgcgcctgg
ttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgaca
gtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctgga
gcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcatgaca
aaagaagggtaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtc
gaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggt
gggcctttctgcgtttatatactagagtcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaa
aagttttagtatcatattcgtgttggattattctgcatttttggggagaatggacttactagagttactgacgaccgcg
ttgcgacatacgcaggggcataaacaggaagcggcgcggctactcggctccccggcgcctactagagccaggcatcaaa
taaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtca
cactggctcaccttcgggtgggcctttctgcgtttata