LOCUS BBa_J64904 4783 bp DNA linear 20-Dec-2024 DEFINITION glnG-lacI oscillator ACCESSION BBa_J64904 VERSION BBa_J64904.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 promoter 64..214 /label=BBa_J64979 misc_feature 225..241 /label=lac O1 promoter 242..247 /label=-35 misc_feature 248..264 /label=lac O1 promoter 265..270 /label=-10 misc_feature 223..276 /label=BBa_R0011 promoter 223..276 /label=BBa_R0011 RBS 286..300 /label=RBS-1\Strong RBS 293..297 /label=RBS misc_feature 286..300 /label=BBa_B0030 RBS 286..300 /label=BBa_B0030 CDS 309..1718 /label=BBa_J64977 stem_loop 1738..1781 /label=stem_loop misc_feature 1727..1806 /label=BBa_B0010 terminator 1727..1806 /label=BBa_B0010 stem_loop 1822..1841 /label=T7 TE misc_feature 1848..1848 /label=stop polyA_site 1842..1855 /label=polya misc_feature 1815..1855 /label=BBa_B0012 terminator 1815..1855 /label=BBa_B0012 promoter 1864..2010 /label=BBa_J64931 RBS 2019..2033 /label=RBS-1\Strong RBS 2026..2030 /label=RBS misc_feature 2019..2033 /label=BBa_B0030 RBS 2019..2033 /label=BBa_B0030 misc_feature 3129..3167 /label=LVA CDS 2040..3167 /label=lacI-LVA misc_feature 2040..3167 /label=BBa_C0012 misc_feature 3168..3192 /label=Help:Barcodes CDS 2040..3167 /label=BBa_C0012 misc_feature 3205..3208 /label=conserved RBS 3201..3212 /label=BBa_B0034 misc_feature 3798..3829 /label=LVA CDS 3219..3797 /label=luxI misc_feature 3219..3836 /label=BBa_C0061 misc_feature 3837..3861 /label=Help:Barcodes CDS 3219..3836 /label=BBa_C0061 misc_feature 3874..3877 /label=conserved RBS 3870..3881 /label=BBa_B0034 misc_feature 3888..4646 /label=BBa_E0044 stem_loop 4666..4709 /label=stem_loop misc_feature 4655..4734 /label=BBa_B0010 terminator 4655..4734 /label=BBa_B0010 stem_loop 4750..4769 /label=T7 TE misc_feature 4776..4776 /label=stop polyA_site 4770..4783 /label=polya misc_feature 4743..4783 /label=BBa_B0012 terminator 4743..4783 /label=BBa_B0012 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagattgcac caacatggtg cttaatgttt ccattgaagc actatattgg tgcaacattc 121 acatcgtggt gcagcccttt tgcacgatgg tgcgcatgat aacgcctttt aggggcaatt 181 taaaagttgg cacagatttc gctttatctt tttttactag agaattgtga gcggataaca 241 attgacattg tgagcggata acaagatact gagcacatac tagagattaa agaggagaaa 301 tactagagtc actccatccc cagctctttt aacttacgcg tcagggtgtt gcggccccag 361 ccgagtagcc gcgccgcttc ctgtttatgc ccctgcgtat gtcgcaacgc ggtcgtcagt 421 aacgtccgct ccagctctgg ctgcgcttcg gaaagcagat tttgatgacc ggaacgcagc 481 gctctgtctg cccactgcgc aagaagcgtc gcccagctgt ccggttgcat ttgcgaagta 541 ctctccgcaa ccgttgattc aaacagttcg ccgggcaaat cctgaatcaa cacttcctgc 601 ccggcggcca tcaccgttag ccagcggcag gtgttttcca gctggcgcac gttgcctggc 661 cacgccagac gcgtcagagc agcttcggtt tccggatgca gtaacttcgc ttctacgccc 721 agttcgcgcg cggcaacctg taaaaaatgg cgcgccagac ggggaatatc ttcccgacgt 781 tcgcgcagcg gcggcagatg aacgcggata acgttcaggc ggtggaacag atcctcacgg 841 aacttacctt cctgcactcg ctgttcgaga ttctggtgag tggcagcgat aatccgcaca 901 tccactttca ccggcgcata gccgccaacg cggtaaaact gaccgtctgc cagcacgcgc 961 agcaaacgcg tctgcacatc cagcggcata tcaccaattt cgtcgaggaa taatgtaccg 1021 ccatcggcct gttcaaaacg cccctgacga atggtattcg cgccagtaaa cgcgcctttc 1081 tcgtggccaa acagttctga ttcgatcaaa tcttttggga tagctgccat attcagcgcg 1141 ataaacggcg ctttggcgcg cggactgtgg cgatgcaggg catgagcgac cagttcttta 1201 ccggtgccgg attcgccgtt aatcagcacg ctaatagaag aacgcgaaag ccgaccgata 1261 atacggaaca cgtcctgcat ggctggcgct tcgccgatga tatcggtcgt tgggccgtta 1321 agctgaacat tacgcggctg ctgctgttcc tggtaatgac tgatagcgcg ctcaaccagc 1381 gccactgctt cgtcgatatc aaacggtttg ggcagataat caaacgcccc ttgttgatag 1441 gcgctgacgg cagcatccag atcggaatgt gcggtcataa tgatgaccgg aagcattgga 1501 tggcgctgtt taatctgctt gagcagcgcc agcccgtcca ttcccggcat acggatatct 1561 gaaagcagca catccggcgt tttgctcgcc agcgcctcca gcacttctgc gccgttctca 1621 aacgtcgtac aggttaaacc tgccccagcg agcgcacgtt caagcaccca acggatggaa 1681 ctatcgtcat cgactaccca gactatccct cgttgcatta ctagagccag gcatcaaata 1741 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 1801 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta 1861 gaggtctgca ctacgtcaac acgaatatga aggataagag cgattatgac gctgacgcac 1921 gctatatcct gtccgtcacc aaatcgtttt aattgtcata ggcttccggc cgcgggtggc 1981 gacaccctgg ccgggtaaca ggagaacccg tactagagat taaagaggag aaatactaga 2041 tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt gtctcttatc 2101 agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag 2161 tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg 2221 gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc 2281 aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga 2341 tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac 2401 gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt gctgtggaag 2461 ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca cccatcaaca 2521 gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg gtcgcattgg 2581 gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc 2641 tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg gaacgggaag 2701 gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat gagggcatcg 2761 ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg cgcgccatta 2821 ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac gataccgaag 2881 acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc ctgctggggc 2941 aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc 3001 tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct 3061 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa 3121 gcgggcaggc tgcaaacgac gaaaactacg ctttagtagc ttaataactc tgatagtgct 3181 agtgtagatc tctactagag aaagaggaga aatactagat gactataatg ataaaaaaat 3241 cggatttttt ggcaattcca tcggaggagt ataaaggtat tctaagtctt cgttatcaag 3301 tgtttaagca aagacttgag tgggacttag ttgtagaaaa taaccttgaa tcagatgagt 3361 atgataactc aaatgcagaa tatatttatg cttgtgatga tactgaaaat gtaagtggat 3421 gctggcgttt attacctaca acaggtgatt atatgctgaa aagtgttttt cctgaattgc 3481 ttggtcaaca gagtgctccc aaagatccta atatagtcga attaagtcgt tttgctgtag 3541 gtaaaaatag ctcaaagata aataactctg ctagtgaaat tacaatgaaa ctatttgaag 3601 ctatatataa acacgctgtt agtcaaggta ttacagaata tgtaacagta acatcaacag 3661 caatagagcg atttttaaag cgtattaaag ttccttgtca tcgtattgga gacaaagaaa 3721 ttcatgtatt aggtgatact aaatcggttg tattgtctat gcctattaat gaacagttta 3781 aaaaagcagt cttaaatgct gcaaacgacg aaaactacgc tttagtagct taataactct 3841 gatagtgcta gtgtagatct ctactagaga aagaggagaa atactagatg cgtaaaggag 3901 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc 3961 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta 4021 aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc actactttcg 4081 gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat gactttttca 4141 agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga 4201 actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt 4261 taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact 4321 ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact 4381 tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa 4441 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat 4501 ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa 4561 cagctgctgg gattacacat ggcatggatg aactatacaa aaggcctgct gcaaacgacg 4621 aaaactacgc tgctgctgtt taataatact agagccaggc atcaaataaa acgaaaggct 4681 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 4741 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata //