##gff-version 3
##sequence-region BBa_J64904 1 4783
BBa_J64904	.	terminator	4655	4734	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J64904	.	stem_loop	4666	4709	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J64904	.	engineered_region	4655	4734	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J64904	.	terminator	1727	1806	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J64904	.	stem_loop	1738	1781	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J64904	.	engineered_region	1727	1806	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J64904	.	ribosome_entry_site	2019	2033	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J64904	.	engineered_region	2019	2033	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J64904	.	ribosome_entry_site	2026	2030	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J64904	.	ribosome_entry_site	2019	2033	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J64904	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_J64904	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J64904	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J64904	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J64904	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J64904	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J64904	.	ribosome_entry_site	3201	3212	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J64904	.	conserved_region	3205	3208	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J64904	.	promoter	1864	2010	.	+	0	ID=BBa_J64931;Name=BBa_J64931
BBa_J64904	.	terminator	1815	1855	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J64904	.	stem_loop	1822	1841	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J64904	.	polyA_site	1842	1855	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J64904	.	stop_codon	1848	1848	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J64904	.	engineered_region	1815	1855	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J64904	.	terminator	4743	4783	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J64904	.	stem_loop	4750	4769	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J64904	.	polyA_site	4770	4783	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J64904	.	stop_codon	4776	4776	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J64904	.	engineered_region	4743	4783	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J64904	.	ribosome_entry_site	3870	3881	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J64904	.	conserved_region	3874	3877	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J64904	.	promoter	223	276	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_J64904	.	operator	248	264	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J64904	.	promoter	242	247	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J64904	.	promoter	265	270	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J64904	.	operator	225	241	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J64904	.	engineered_region	223	276	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J64904	.	promoter	64	214	.	+	0	ID=BBa_J64979;Name=BBa_J64979
BBa_J64904	.	engineered_region	3888	4646	.	+	0	ID=BBa_E0044;Name=BBa_E0044
BBa_J64904	.	CDS	3219	3836	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_J64904	.	engineered_region	3219	3836	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_J64904	.	engineered_tag	3837	3861	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_J64904	.	CDS	3219	3797	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_J64904	.	tag	3798	3829	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_J64904	.	CDS	2040	3167	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_J64904	.	engineered_tag	3168	3192	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_J64904	.	CDS	2040	3167	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_J64904	.	engineered_region	2040	3167	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_J64904	.	tag	3129	3167	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_J64904	.	ribosome_entry_site	286	300	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J64904	.	engineered_region	286	300	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J64904	.	ribosome_entry_site	293	297	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J64904	.	ribosome_entry_site	286	300	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J64904	.	CDS	309	1718	.	+	0	ID=BBa_J64977;Name=BBa_J64977
>BBa_J64904
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagattgcaccaacatggt
gcttaatgtttccattgaagcactatattggtgcaacattcacatcgtggtgcagcccttttgcacgatggtgcgcatg
ataacgccttttaggggcaatttaaaagttggcacagatttcgctttatctttttttactagagaattgtgagcggata
acaattgacattgtgagcggataacaagatactgagcacatactagagattaaagaggagaaatactagagtcactcca
tccccagctcttttaacttacgcgtcagggtgttgcggccccagccgagtagccgcgccgcttcctgtttatgcccctg
cgtatgtcgcaacgcggtcgtcagtaacgtccgctccagctctggctgcgcttcggaaagcagattttgatgaccggaa
cgcagcgctctgtctgcccactgcgcaagaagcgtcgcccagctgtccggttgcatttgcgaagtactctccgcaaccg
ttgattcaaacagttcgccgggcaaatcctgaatcaacacttcctgcccggcggccatcaccgttagccagcggcaggt
gttttccagctggcgcacgttgcctggccacgccagacgcgtcagagcagcttcggtttccggatgcagtaacttcgct
tctacgcccagttcgcgcgcggcaacctgtaaaaaatggcgcgccagacggggaatatcttcccgacgttcgcgcagcg
gcggcagatgaacgcggataacgttcaggcggtggaacagatcctcacggaacttaccttcctgcactcgctgttcgag
attctggtgagtggcagcgataatccgcacatccactttcaccggcgcatagccgccaacgcggtaaaactgaccgtct
gccagcacgcgcagcaaacgcgtctgcacatccagcggcatatcaccaatttcgtcgaggaataatgtaccgccatcgg
cctgttcaaaacgcccctgacgaatggtattcgcgccagtaaacgcgcctttctcgtggccaaacagttctgattcgat
caaatcttttgggatagctgccatattcagcgcgataaacggcgctttggcgcgcggactgtggcgatgcagggcatga
gcgaccagttctttaccggtgccggattcgccgttaatcagcacgctaatagaagaacgcgaaagccgaccgataatac
ggaacacgtcctgcatggctggcgcttcgccgatgatatcggtcgttgggccgttaagctgaacattacgcggctgctg
ctgttcctggtaatgactgatagcgcgctcaaccagcgccactgcttcgtcgatatcaaacggtttgggcagataatca
aacgccccttgttgataggcgctgacggcagcatccagatcggaatgtgcggtcataatgatgaccggaagcattggat
ggcgctgtttaatctgcttgagcagcgccagcccgtccattcccggcatacggatatctgaaagcagcacatccggcgt
tttgctcgccagcgcctccagcacttctgcgccgttctcaaacgtcgtacaggttaaacctgccccagcgagcgcacgt
tcaagcacccaacggatggaactatcgtcatcgactacccagactatccctcgttgcattactagagccaggcatcaaa
taaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtca
cactggctcaccttcgggtgggcctttctgcgtttatatactagaggtctgcactacgtcaacacgaatatgaaggata
agagcgattatgacgctgacgcacgctatatcctgtccgtcaccaaatcgttttaattgtcataggcttccggccgcgg
gtggcgacaccctggccgggtaacaggagaacccgtactagagattaaagaggagaaatactagatggtgaatgtgaaa
ccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagcc
acgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaaca
actggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcg
gcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgta
aagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccat
tgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattatt
ttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgg
gcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgat
agcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttccc
actgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtg
cggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacagga
ttttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctg
ttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgatt
cattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagc
ttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatgactataatgataaaaaaa
tcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttg
agtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtga
tgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaa
ttgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaa
agataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattac
agaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagac
aaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtct
taaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaa
agaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgat
gttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttattt
gcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagata
cccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttc
aaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaag
gtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcat
ggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagca
gaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctg
ccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatgg
catggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctgctgctgtttaataatactagagccaggca
tcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactag
agtcacactggctcaccttcgggtgggcctttctgcgtttata