LOCUS       BBa_J64974              1605 bp    DNA     linear       22-Nov-2024
DEFINITION  small molecule controlled, riboregulator based XOR gate
ACCESSION   BBa_J64974
VERSION     BBa_J64974.1  
FEATURES             Location/Qualifiers
     misc_feature    1..37
                     /label=P(Lac) IQ
     promoter        3..8
                     /label=-35
     promoter        26..31
                     /label=-10
     promoter        1..37
                     /label=BBa_I14032
     misc_feature    46..149
                     /label=BBa_J64720
     stem_loop       169..212
                     /label=stem_loop
     misc_feature    158..237
                     /label=BBa_B0010
     terminator      158..237
                     /label=BBa_B0010
     stem_loop       253..272
                     /label=T7 TE
     misc_feature    279..279
                     /label=stop
     polyA_site      273..286
                     /label=polya
     misc_feature    246..286
                     /label=BBa_B0012
     terminator      246..286
                     /label=BBa_B0012
     misc_feature    295..313
                     /label=TetR 1
     misc_feature    295..348
                     /label=BBa_R0040
     promoter        314..319
                     /label=-35
     misc_feature    320..338
                     /label=TetR 2
     promoter        337..342
                     /label=-10
     promoter        295..348
                     /label=BBa_R0040
     misc_feature    357..460
                     /label=BBa_J64722
     stem_loop       480..523
                     /label=stem_loop
     misc_feature    469..548
                     /label=BBa_B0010
     terminator      469..548
                     /label=BBa_B0010
     stem_loop       564..583
                     /label=T7 TE
     misc_feature    590..590
                     /label=stop
     polyA_site      584..597
                     /label=polya
     misc_feature    557..597
                     /label=BBa_B0012
     terminator      557..597
                     /label=BBa_B0012
     promoter        606..640
                     /label=BBa_J23100
     misc_feature    649..700
                     /label=BBa_J64723
     CDS             707..1468
                     /label=CFP (LVA)
     misc_feature    1425..1462
                     /label=SsrA
     misc_feature    1463..1468
                     /label=2
     misc_feature    775..775
                     /label=A
     misc_feature    707..1468
                     /label=BBa_E0022
     misc_feature    707..1468
                     /label=BBa_E0022
     stem_loop       1488..1531
                     /label=stem_loop
     misc_feature    1477..1556
                     /label=BBa_B0010
     terminator      1477..1556
                     /label=BBa_B0010
     stem_loop       1572..1591
                     /label=T7 TE
     misc_feature    1598..1598
                     /label=stop
     polyA_site      1592..1605
                     /label=polya
     misc_feature    1565..1605
                     /label=BBa_B0012
     terminator      1565..1605
                     /label=BBa_B0012
ORIGIN
        1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagcggcg acaggagcct
       61 ctagagatat atggtatagt aagttaattt tcattaacca ccaacccaaa tccaggaggt
      121 gattggtaga ccctagcgat gctcgaacct actagagcca ggcatcaaat aaaacgaaag
      181 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
      241 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta
      301 tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacta ctagagggtt
      361 cgagcatcgc tagggtaccc aaatccagga ggtgattggt agtggtggtt aatgaaaatt
      421 aacttactat accatatatc tctagaggct cctgtcgccg tactagagcc aggcatcaaa
      481 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
      541 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac
      601 tagagttgac ggctagctca gtcctaggta cagtgctagc tactagagga attctaccat
      661 tcacctcttg gatttgggta ttaaagagga gaaaggtacc tactagatgg tgagcaaggg
      721 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtgaacgg
      781 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
      841 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
      901 gacctggggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
      961 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     1021 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     1081 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     1141 ctacatcagc cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa
     1201 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     1261 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     1321 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     1381 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagaggcctg ctgcaaacga
     1441 cgaaaactac gctttagtag cttaataata ctagagccag gcatcaaata aaacgaaagg
     1501 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     1561 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//