##gff-version 3
##sequence-region BBa_J64974 1 1605
BBa_J64974	.	mature_transcript_region	357	460	.	+	0	ID=BBa_J64722;Name=BBa_J64722
BBa_J64974	.	terminator	1477	1556	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J64974	.	engineered_region	1477	1556	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J64974	.	stem_loop	1488	1531	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J64974	.	terminator	246	286	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J64974	.	polyA_site	273	286	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J64974	.	engineered_region	246	286	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J64974	.	stem_loop	253	272	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J64974	.	stop_codon	279	279	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J64974	.	promoter	295	348	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J64974	.	engineered_region	295	348	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J64974	.	promoter	314	319	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J64974	.	promoter	337	342	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J64974	.	non_covalent_binding_site	295	313	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J64974	.	non_covalent_binding_site	320	338	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J64974	.	terminator	469	548	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J64974	.	engineered_region	469	548	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J64974	.	stem_loop	480	523	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J64974	.	mature_transcript_region	46	149	.	+	0	ID=BBa_J64720;Name=BBa_J64720
BBa_J64974	.	terminator	1565	1605	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J64974	.	polyA_site	1592	1605	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J64974	.	engineered_region	1565	1605	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J64974	.	stem_loop	1572	1591	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J64974	.	stop_codon	1598	1598	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J64974	.	mature_transcript_region	649	700	.	+	0	ID=BBa_J64723;Name=BBa_J64723
BBa_J64974	.	engineered_region	707	1468	.	+	0	ID=BBa_E0022;Name=BBa_E0022
BBa_J64974	.	point_mutation	775	775	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_J64974	.	tag	1425	1462	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_J64974	.	CDS	707	1468	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_J64974	.	stop_codon	1463	1468	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_J64974	.	engineered_region	707	1468	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_J64974	.	promoter	1	37	.	+	0	ID=BBa_I14032;Name=BBa_I14032
BBa_J64974	.	operator	1	37	.	+	0	ID=annotation1028342;Name=P(Lac) IQ;Parent=BBa_I14032
BBa_J64974	.	promoter	3	8	.	+	0	ID=annotation1028343;Name=-35;Parent=BBa_I14032
BBa_J64974	.	promoter	26	31	.	+	0	ID=annotation1028344;Name=-10;Parent=BBa_I14032
BBa_J64974	.	promoter	606	640	.	+	0	ID=BBa_J23100;Name=BBa_J23100
BBa_J64974	.	terminator	158	237	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J64974	.	engineered_region	158	237	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J64974	.	stem_loop	169	212	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J64974	.	terminator	557	597	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J64974	.	polyA_site	584	597	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J64974	.	engineered_region	557	597	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J64974	.	stem_loop	564	583	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J64974	.	stop_codon	590	590	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
>BBa_J64974
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagcggcgacaggagcctctagagatatatggtatag
taagttaattttcattaaccaccaacccaaatccaggaggtgattggtagaccctagcgatgctcgaacctactagagc
caggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
tactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattg
acatccctatcagtgatagagatactgagcactactagagggttcgagcatcgctagggtacccaaatccaggaggtga
ttggtagtggtggttaatgaaaattaacttactataccatatatctctagaggctcctgtcgccgtactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagtcctaggtaca
gtgctagctactagaggaattctaccattcacctcttggatttgggtattaaagaggagaaaggtacctactagatggt
gagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttc
agcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgc
ccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagca
gcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactac
aagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggagg
acggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaa
cggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaac
acccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagacccca
acgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaa
gaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggc
tcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacct
tcgggtgggcctttctgcgtttata