BBa_J64974
type: ComponentDefinition
persistent identity: BBa J64974
display id: BBa_J64974
version: 1
was generated by: iGEM to SBOL conversion
description: small molecule controlled, riboregulator based XOR gate
created: 2007-03-20T12:00:00Z
modified: 2015-05-08T01:08:17Z
mutable provenance: The Collins lab riboregulators and existing registry parts.
owned by: james
owned by: myers
mutable description: This device uses the small molecules lac and tet as inputs and expresses GFP as an XOR function of the two.
discontinued: false
dominant: false
group u list: _98_
m user id: 0
owner id: 1432
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The presence of either (but not both) of the keys unlocks a blocked RBS to allow translation of a CFP degradation tag fusion. The two keys have a greater affinity to each other than to the lock, so the presence of both does not unlock the blocked RBS.
star: false
creator: reid williams
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J64720
component: BBa_I14032
component: BBa_B0012
component: BBa_B0010
component: BBa_J64723
component: BBa_J23100
component: BBa_E0022
component: BBa_J64722
component: BBa_R0040
sequence annotation: BBa_I14032
sequence annotation: BBa_J64720
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_J64722
sequence annotation: BBa_J23100
sequence annotation: BBa_J64723
sequence annotation: BBa_E0022
sequence: https://synbiohub.org/public/igem/BBa_J64974_sequence/1

BBa_J64720
type: Component
persistent identity: component1921323
display id: component1921323
version: 1
top level: BBa_J64974
definition: BBa_J64720
access: public

BBa_I14032
type: Component
persistent identity: component1921322
display id: component1921322
version: 1
top level: BBa_J64974
definition: P(Lac) IQ
access: public

BBa_B0012
type: Component
persistent identity: component1921326
display id: component1921326
version: 1
top level: BBa_J64974
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1921324
display id: component1921324
version: 1
top level: BBa_J64974
definition: BBa_B0010
access: public

BBa_J64723
type: Component
persistent identity: component1921343
display id: component1921343
version: 1
top level: BBa_J64974
definition: BBa_J64723
access: public

BBa_J23100
type: Component
persistent identity: component1921342
display id: component1921342
version: 1
top level: BBa_J64974
definition: BBa_J23100
access: public

BBa_B0010
type: Component
persistent identity: component1921349
display id: component1921349
version: 1
top level: BBa_J64974
definition: BBa_B0010
access: public

BBa_E0022
type: Component
persistent identity: component1921345
display id: component1921345
version: 1
top level: BBa_J64974
definition: ECFP
access: public

BBa_J64722
type: Component
persistent identity: component1921335
display id: component1921335
version: 1
top level: BBa_J64974
definition: BBa_J64722
access: public

BBa_R0040
type: Component
persistent identity: component1921331
display id: component1921331
version: 1
top level: BBa_J64974
definition: p(tetR)
access: public

BBa_B0012
type: Component
persistent identity: component1921338
display id: component1921338
version: 1
top level: BBa_J64974
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1921336
display id: component1921336
version: 1
top level: BBa_J64974
definition: BBa_B0010
access: public

BBa_B0012
type: Component
persistent identity: component1921351
display id: component1921351
version: 1
top level: BBa_J64974
definition: BBa_B0012
access: public

BBa_I14032
type: SequenceAnnotation
persistent identity: annotation1921322
display id: annotation1921322
version: 1
top level: BBa_J64974
location: https://synbiohub.org/public/igem/BBa_J64974/annotation1921322/range1921322/1
component: BBa_I14032

https://synbiohub.org/public/igem/BBa_J64974/annotation1921322/range1921322/1
type: Range
persistent identity: range1921322
display id: range1921322
version: 1
top level: BBa_J64974
direction: direction/reverse
start: 1
end: 37
orientation: inline

BBa_J64720
type: SequenceAnnotation
persistent identity: annotation1921323
display id: annotation1921323
version: 1
top level: BBa_J64974
location: https://synbiohub.org/public/igem/BBa_J64974/annotation1921323/range1921323/1
component: BBa_J64720

https://synbiohub.org/public/igem/BBa_J64974/annotation1921323/range1921323/1
type: Range
persistent identity: range1921323
display id: range1921323
version: 1
top level: BBa_J64974
direction: direction/reverse
start: 46
end: 149
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1921324
display id: annotation1921324
version: 1
top level: BBa_J64974
location: https://synbiohub.org/public/igem/BBa_J64974/annotation1921324/range1921324/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J64974/annotation1921324/range1921324/1
type: Range
persistent identity: range1921324
display id: range1921324
version: 1
top level: BBa_J64974
direction: direction/forward
start: 158
end: 237
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1921326
display id: annotation1921326
version: 1
top level: BBa_J64974
location: https://synbiohub.org/public/igem/BBa_J64974/annotation1921326/range1921326/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J64974/annotation1921326/range1921326/1
type: Range
persistent identity: range1921326
display id: range1921326
version: 1
top level: BBa_J64974
direction: direction/forward
start: 246
end: 286
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1921331
display id: annotation1921331
version: 1
top level: BBa_J64974
location: https://synbiohub.org/public/igem/BBa_J64974/annotation1921331/range1921331/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J64974/annotation1921331/range1921331/1
type: Range
persistent identity: range1921331
display id: range1921331
version: 1
top level: BBa_J64974
direction: direction/forward
start: 295
end: 348
orientation: inline

BBa_J64722
type: SequenceAnnotation
persistent identity: annotation1921335
display id: annotation1921335
version: 1
top level: BBa_J64974
location: https://synbiohub.org/public/igem/BBa_J64974/annotation1921335/range1921335/1
component: BBa_J64722

https://synbiohub.org/public/igem/BBa_J64974/annotation1921335/range1921335/1
type: Range
persistent identity: range1921335
display id: range1921335
version: 1
top level: BBa_J64974
direction: direction/reverse
start: 357
end: 460
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1921336
display id: annotation1921336
version: 1
top level: BBa_J64974
location: https://synbiohub.org/public/igem/BBa_J64974/annotation1921336/range1921336/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J64974/annotation1921336/range1921336/1
type: Range
persistent identity: range1921336
display id: range1921336
version: 1
top level: BBa_J64974
direction: direction/forward
start: 469
end: 548
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1921338
display id: annotation1921338
version: 1
top level: BBa_J64974
location: https://synbiohub.org/public/igem/BBa_J64974/annotation1921338/range1921338/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J64974/annotation1921338/range1921338/1
type: Range
persistent identity: range1921338
display id: range1921338
version: 1
top level: BBa_J64974
direction: direction/forward
start: 557
end: 597
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation1921342
display id: annotation1921342
version: 1
top level: BBa_J64974
location: https://synbiohub.org/public/igem/BBa_J64974/annotation1921342/range1921342/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_J64974/annotation1921342/range1921342/1
type: Range
persistent identity: range1921342
display id: range1921342
version: 1
top level: BBa_J64974
direction: direction/reverse
start: 606
end: 640
orientation: inline

BBa_J64723
type: SequenceAnnotation
persistent identity: annotation1921343
display id: annotation1921343
version: 1
top level: BBa_J64974
location: https://synbiohub.org/public/igem/BBa_J64974/annotation1921343/range1921343/1
component: BBa_J64723

https://synbiohub.org/public/igem/BBa_J64974/annotation1921343/range1921343/1
type: Range
persistent identity: range1921343
display id: range1921343
version: 1
top level: BBa_J64974
direction: direction/reverse
start: 649
end: 700
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation1921345
display id: annotation1921345
version: 1
top level: BBa_J64974
location: https://synbiohub.org/public/igem/BBa_J64974/annotation1921345/range1921345/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_J64974/annotation1921345/range1921345/1
type: Range
persistent identity: range1921345
display id: range1921345
version: 1
top level: BBa_J64974
direction: direction/forward
start: 707
end: 1468
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1921349
display id: annotation1921349
version: 1
top level: BBa_J64974
location: https://synbiohub.org/public/igem/BBa_J64974/annotation1921349/range1921349/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J64974/annotation1921349/range1921349/1
type: Range
persistent identity: range1921349
display id: range1921349
version: 1
top level: BBa_J64974
direction: direction/forward
start: 1477
end: 1556
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1921351
display id: annotation1921351
version: 1
top level: BBa_J64974
location: https://synbiohub.org/public/igem/BBa_J64974/annotation1921351/range1921351/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J64974/annotation1921351/range1921351/1
type: Range
persistent identity: range1921351
display id: range1921351
version: 1
top level: BBa_J64974
direction: direction/forward
start: 1565
end: 1605
orientation: inline

BBa_J64720
type: ComponentDefinition
persistent identity: BBa J64720
display id: BBa_J64720
version: 1
was generated by: iGEM to SBOL conversion
description: taR12 prime key
created: 2007-03-20T12:00:00Z
modified: 2015-05-08T01:08:17Z
mutable provenance: dfjls
owned by: james
owned by: myers
mutable description: dlfa
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1425
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: dsjkl
star: false
creator: Sheel Dandekar
type: DnaRegion
role: partType/RNA
role: SO:0000834
sequence: https://synbiohub.org/public/igem/BBa_J64720_sequence/1

BBa_J64722
type: ComponentDefinition
persistent identity: BBa J64722
display id: BBa_J64722
version: 1
was generated by: iGEM to SBOL conversion
description: taR12 key
created: 2007-03-20T12:00:00Z
modified: 2015-05-08T01:08:17Z
mutable provenance: sadfl
owned by: james
owned by: myers
mutable description: dsf;
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1425
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: sdjaf
star: false
creator: Sheel Dandekar
type: DnaRegion
role: partType/RNA
role: SO:0000834
sequence: https://synbiohub.org/public/igem/BBa_J64722_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

P(Lac) IQ
type: ComponentDefinition
persistent identity: BBa I14032
display id: BBa_I14032
version: 1
was generated by: iGEM to SBOL conversion
description: promoter P(Lac) IQ
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:37Z
mutable provenance: Plasmid pMAL-p2X
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Constitutive Promoter, High Transcription
discontinued: false
dominant: true
experience: experience/Works
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: P(Lac) IQ
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I14032_sequence/1

P(Lac) IQ
type: SequenceAnnotation
persistent identity: annotation1028342
display id: annotation1028342
version: 1
top level: P(Lac) IQ
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028342/range1028342/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_I14032/annotation1028342/range1028342/1
type: Range
persistent identity: range1028342
display id: range1028342
version: 1
top level: P(Lac) IQ
direction: direction/forward
start: 1
end: 37
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1028343
display id: annotation1028343
version: 1
top level: P(Lac) IQ
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028343/range1028343/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I14032/annotation1028343/range1028343/1
type: Range
persistent identity: range1028343
display id: range1028343
version: 1
top level: P(Lac) IQ
direction: direction/forward
start: 3
end: 8
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1028344
display id: annotation1028344
version: 1
top level: P(Lac) IQ
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028344/range1028344/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I14032/annotation1028344/range1028344/1
type: Range
persistent identity: range1028344
display id: range1028344
version: 1
top level: P(Lac) IQ
direction: direction/forward
start: 26
end: 31
orientation: inline

BBa_J64723
type: ComponentDefinition
persistent identity: BBa J64723
display id: BBa_J64723
version: 1
was generated by: iGEM to SBOL conversion
description: crR12 lock
created: 2007-03-20T12:00:00Z
modified: 2015-05-08T01:08:17Z
mutable provenance: dljk
owned by: james
owned by: myers
mutable description: ksfldj
discontinued: false
dominant: false
group u list: _98_
m user id: 0
owner id: 1425
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: dfsl
star: false
creator: Sheel Dandekar
type: DnaRegion
role: partType/RNA
role: SO:0000834
sequence: https://synbiohub.org/public/igem/BBa_J64723_sequence/1

ECFP
type: ComponentDefinition
persistent identity: BBa E0022
display id: BBa_E0022
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced cyan fluorescent protein derived from A. victoria GFP
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modified from <bb_part>BBa_E0021</bb_part>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Cyan fluorescent protein (ECFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_E0022 cyan fluorescent protein is based on BioBrick part BBa_E0021. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_E0022
sequence annotation: CFP (LVA)
sequence annotation: A
sequence annotation: SsrA
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_E0022_sequence/1

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation7040
display id: annotation7040
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
type: Range
persistent identity: range7040
display id: range7040
version: 1
top level: ECFP
direction: direction/forward
start: 1
end: 762
orientation: inline

CFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2150
display id: annotation2150
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
type: Range
persistent identity: range2150
display id: range2150
version: 1
top level: ECFP
direction: direction/forward
start: 1
end: 762
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation2155
display id: annotation2155
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
type: Range
persistent identity: range2155
display id: range2155
version: 1
top level: ECFP
direction: direction/forward
start: 69
end: 69
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation2153
display id: annotation2153
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
type: Range
persistent identity: range2153
display id: range2153
version: 1
top level: ECFP
direction: direction/forward
start: 719
end: 756
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2154
display id: annotation2154
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
type: Range
persistent identity: range2154
display id: range2154
version: 1
top level: ECFP
direction: direction/forward
start: 757
end: 762
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

https://synbiohub.org/public/igem/BBa_J64974_sequence/1
type: Sequence
persistent identity: BBa J64974 sequence
display id: BBa_J64974_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagcggcgacaggagcctctagagatatatggtatagtaagttaattttcattaaccaccaacccaaatccaggaggtgattggtagaccctagcgatgctcgaacctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagggttcgagcatcgctagggtacccaaatccaggaggtgattggtagtggtggttaatgaaaattaacttactataccatatatctctagaggctcctgtcgccgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagtcctaggtacagtgctagctactagaggaattctaccattcacctcttggatttgggtattaaagaggagaaaggtacctactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J64720_sequence/1
type: Sequence
persistent identity: BBa J64720 sequence
display id: BBa_J64720_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cggcgacaggagcctctagagatatatggtatagtaagttaattttcattaaccaccaacccaaatccaggaggtgattggtagaccctagcgatgctcgaacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J64722_sequence/1
type: Sequence
persistent identity: BBa J64722 sequence
display id: BBa_J64722_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggttcgagcatcgctagggtacccaaatccaggaggtgattggtagtggtggttaatgaaaattaacttactataccatatatctctagaggctcctgtcgccg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J64723_sequence/1
type: Sequence
persistent identity: BBa J64723 sequence
display id: BBa_J64723_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaattctaccattcacctcttggatttgggtattaaagaggagaaaggtacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I14032_sequence/1
type: Sequence
persistent identity: BBa I14032 sequence
display id: BBa_I14032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0022_sequence/1
type: Sequence
persistent identity: BBa E0022 sequence
display id: BBa_E0022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z