BBa_J64975
1
BBa_J64975
anti-glnG RNAi
2007-03-23T12:00:00Z
2015-05-08T01:08:17Z
''E. coli'' K-12
Cognate RNAi for glnG from -40 to +24. Last 10 bases on 3' end are not involved in binding with glnG mRNA, but rather serve as a binding site for an additional RNAi
false
false
_98_
0
1429
98
Not in stock
false
Wanted an RNAi that would overlap the RBS and start codon for glnG. As an additional constraint on the design, also wanted to allow regulation of the cytosolic levels of this RNAi via an additional RNAi. This was accomplished by extending the 3' end with a G/C-rich region.
false
Kristopher Kuchenbecker
BBa_J64975_sequence
1
ttactgacgaccgcgttgcgacatacgcaggggcataaacaggaagcggcgcggctactcggctccccggcgcc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z