LOCUS       BBa_J64990              2479 bp    DNA     linear       23-Dec-2024
DEFINITION  AHL driven glnG
ACCESSION   BBa_J64990
VERSION     BBa_J64990.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     terminator      870..998
                     /label=BBa_B0015
     misc_feature    1007..1026
                     /label=LuxR/HSL
     promoter        1026..1031
                     /label=-35
     promoter        1048..1053
                     /label=-10
     misc_feature    1059..1059
                     /label=start
     misc_feature    1007..1061
                     /label=BBa_R0062
     promoter        1007..1061
                     /label=BBa_R0062
     misc_feature    1..1061
                     /label=BBa_F2620
     CDS             1070..2479
                     /label=BBa_J64977
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt
     1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagagt cactccatcc
     1081 ccagctcttt taacttacgc gtcagggtgt tgcggcccca gccgagtagc cgcgccgctt
     1141 cctgtttatg cccctgcgta tgtcgcaacg cggtcgtcag taacgtccgc tccagctctg
     1201 gctgcgcttc ggaaagcaga ttttgatgac cggaacgcag cgctctgtct gcccactgcg
     1261 caagaagcgt cgcccagctg tccggttgca tttgcgaagt actctccgca accgttgatt
     1321 caaacagttc gccgggcaaa tcctgaatca acacttcctg cccggcggcc atcaccgtta
     1381 gccagcggca ggtgttttcc agctggcgca cgttgcctgg ccacgccaga cgcgtcagag
     1441 cagcttcggt ttccggatgc agtaacttcg cttctacgcc cagttcgcgc gcggcaacct
     1501 gtaaaaaatg gcgcgccaga cggggaatat cttcccgacg ttcgcgcagc ggcggcagat
     1561 gaacgcggat aacgttcagg cggtggaaca gatcctcacg gaacttacct tcctgcactc
     1621 gctgttcgag attctggtga gtggcagcga taatccgcac atccactttc accggcgcat
     1681 agccgccaac gcggtaaaac tgaccgtctg ccagcacgcg cagcaaacgc gtctgcacat
     1741 ccagcggcat atcaccaatt tcgtcgagga ataatgtacc gccatcggcc tgttcaaaac
     1801 gcccctgacg aatggtattc gcgccagtaa acgcgccttt ctcgtggcca aacagttctg
     1861 attcgatcaa atcttttggg atagctgcca tattcagcgc gataaacggc gctttggcgc
     1921 gcggactgtg gcgatgcagg gcatgagcga ccagttcttt accggtgccg gattcgccgt
     1981 taatcagcac gctaatagaa gaacgcgaaa gccgaccgat aatacggaac acgtcctgca
     2041 tggctggcgc ttcgccgatg atatcggtcg ttgggccgtt aagctgaaca ttacgcggct
     2101 gctgctgttc ctggtaatga ctgatagcgc gctcaaccag cgccactgct tcgtcgatat
     2161 caaacggttt gggcagataa tcaaacgccc cttgttgata ggcgctgacg gcagcatcca
     2221 gatcggaatg tgcggtcata atgatgaccg gaagcattgg atggcgctgt ttaatctgct
     2281 tgagcagcgc cagcccgtcc attcccggca tacggatatc tgaaagcagc acatccggcg
     2341 ttttgctcgc cagcgcctcc agcacttctg cgccgttctc aaacgtcgta caggttaaac
     2401 ctgccccagc gagcgcacgt tcaagcaccc aacggatgga actatcgtca tcgactaccc
     2461 agactatccc tcgttgcat
//