BBa_J64994
type: ComponentDefinition
persistent identity: BBa J64994
display id: BBa_J64994
version: 1
was generated by: iGEM to SBOL conversion
description: light synchronizable phase adjustable oscillator
created: 2007-03-25T11:00:00Z
modified: 2015-05-08T01:08:18Z
mutable provenance: null
owned by: james
owned by: myers
mutable description: null
discontinued: false
dominant: false
group u list: _98_
m user id: 0
owner id: 1432
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: null
star: false
creator: reid williams
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J64990
component: BBa_J64904
component: BBa_J64903
sequence annotation: BBa_J64990
sequence annotation: BBa_J64903
sequence annotation: BBa_J64904
sequence: https://synbiohub.org/public/igem/BBa_J64994_sequence/1

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BBa_J64990
type: ComponentDefinition
persistent identity: BBa J64990
display id: BBa_J64990
version: 1
was generated by: iGEM to SBOL conversion
description: AHL driven glnG
created: 2007-03-25T11:00:00Z
modified: 2015-05-08T01:08:18Z
mutable provenance: ''E. coli''
owned by: james
owned by: myers
mutable description: This part places glnG under the control of AHL - luxR mediated cell-cell signaling.
discontinued: false
dominant: false
group u list: _98_
m user id: 0
owner id: 1429
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: n/a
star: false
creator: Kristopher Kuchenbecker
type: DnaRegion
role: SO:0000804
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component: BBa_F2620
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sequence: https://synbiohub.org/public/igem/BBa_J64990_sequence/1

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BBa_J64903
type: ComponentDefinition
persistent identity: BBa J64903
display id: BBa_J64903
version: 1
was generated by: iGEM to SBOL conversion
description: Light induced production of RNAi for glnG
created: 2007-03-25T11:00:00Z
modified: 2015-05-08T01:08:17Z
mutable provenance: ''E. coli''
owned by: james
owned by: myers
mutable description: Combines the light sensitive envZ-ompR two component system with an anti-glnG output.
discontinued: false
dominant: false
group u list: _98_
m user id: 0
owner id: 1429
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: n/a
star: false
creator: Kristopher Kuchenbecker
type: DnaRegion
role: SO:0000804
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BBa_B0012
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BBa_B0012
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BBa_J64904
type: ComponentDefinition
persistent identity: BBa J64904
display id: BBa_J64904
version: 1
was generated by: iGEM to SBOL conversion
description: glnG-lacI oscillator
created: 2007-03-25T11:00:00Z
modified: 2015-05-08T01:08:17Z
mutable provenance: ''E. coli''
owned by: james
owned by: myers
mutable description: Coupling the lac and ntr systems, this part produces oscillations.
discontinued: false
dominant: false
group u list: _98_
m user id: 0
owner id: 1429
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: n/a
star: false
creator: Kristopher Kuchenbecker
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_E0044
component: BBa_B0034
component: BBa_C0061
component: BBa_C0012
component: BBa_B0030
component: BBa_J64931
component: BBa_J64977
component: BBa_R0011
component: BBa_J64979
sequence annotation: BBa_R0011
sequence annotation: BBa_J64979
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sequence annotation: BBa_B0012
sequence annotation: BBa_J64931
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sequence annotation: BBa_B0034
sequence annotation: BBa_C0061
sequence annotation: BBa_E0044
sequence: https://synbiohub.org/public/igem/BBa_J64904_sequence/1

BBa_B0012
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BBa_C0061
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BBa_B0034
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BBa_C0012
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BBa_B0030
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location: https://synbiohub.org/public/igem/BBa_J64904/annotation2313321/range2313321/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J64904/annotation2313321/range2313321/1
type: Range
persistent identity: range2313321
display id: range2313321
version: 1
top level: BBa_J64904
direction: direction/forward
start: 1727
end: 1806
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2313323
display id: annotation2313323
version: 1
top level: BBa_J64904
location: https://synbiohub.org/public/igem/BBa_J64904/annotation2313323/range2313323/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J64904/annotation2313323/range2313323/1
type: Range
persistent identity: range2313323
display id: range2313323
version: 1
top level: BBa_J64904
direction: direction/forward
start: 1815
end: 1855
orientation: inline

BBa_J64931
type: SequenceAnnotation
persistent identity: annotation2313327
display id: annotation2313327
version: 1
top level: BBa_J64904
location: https://synbiohub.org/public/igem/BBa_J64904/annotation2313327/range2313327/1
component: BBa_J64931

https://synbiohub.org/public/igem/BBa_J64904/annotation2313327/range2313327/1
type: Range
persistent identity: range2313327
display id: range2313327
version: 1
top level: BBa_J64904
direction: direction/reverse
start: 1864
end: 2010
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2313329
display id: annotation2313329
version: 1
top level: BBa_J64904
location: https://synbiohub.org/public/igem/BBa_J64904/annotation2313329/range2313329/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J64904/annotation2313329/range2313329/1
type: Range
persistent identity: range2313329
display id: range2313329
version: 1
top level: BBa_J64904
direction: direction/forward
start: 2019
end: 2033
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2313332
display id: annotation2313332
version: 1
top level: BBa_J64904
location: https://synbiohub.org/public/igem/BBa_J64904/annotation2313332/range2313332/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_J64904/annotation2313332/range2313332/1
type: Range
persistent identity: range2313332
display id: range2313332
version: 1
top level: BBa_J64904
direction: direction/forward
start: 2040
end: 3167
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2313336
display id: annotation2313336
version: 1
top level: BBa_J64904
location: https://synbiohub.org/public/igem/BBa_J64904/annotation2313336/range2313336/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J64904/annotation2313336/range2313336/1
type: Range
persistent identity: range2313336
display id: range2313336
version: 1
top level: BBa_J64904
direction: direction/reverse
start: 3201
end: 3212
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation2313338
display id: annotation2313338
version: 1
top level: BBa_J64904
location: https://synbiohub.org/public/igem/BBa_J64904/annotation2313338/range2313338/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_J64904/annotation2313338/range2313338/1
type: Range
persistent identity: range2313338
display id: range2313338
version: 1
top level: BBa_J64904
direction: direction/forward
start: 3219
end: 3836
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2313342
display id: annotation2313342
version: 1
top level: BBa_J64904
location: https://synbiohub.org/public/igem/BBa_J64904/annotation2313342/range2313342/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J64904/annotation2313342/range2313342/1
type: Range
persistent identity: range2313342
display id: range2313342
version: 1
top level: BBa_J64904
direction: direction/reverse
start: 3870
end: 3881
orientation: inline

BBa_E0044
type: SequenceAnnotation
persistent identity: annotation2313343
display id: annotation2313343
version: 1
top level: BBa_J64904
location: https://synbiohub.org/public/igem/BBa_J64904/annotation2313343/range2313343/1
component: BBa_E0044

https://synbiohub.org/public/igem/BBa_J64904/annotation2313343/range2313343/1
type: Range
persistent identity: range2313343
display id: range2313343
version: 1
top level: BBa_J64904
direction: direction/reverse
start: 3888
end: 4646
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2313344
display id: annotation2313344
version: 1
top level: BBa_J64904
location: https://synbiohub.org/public/igem/BBa_J64904/annotation2313344/range2313344/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J64904/annotation2313344/range2313344/1
type: Range
persistent identity: range2313344
display id: range2313344
version: 1
top level: BBa_J64904
direction: direction/forward
start: 4655
end: 4734
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2313346
display id: annotation2313346
version: 1
top level: BBa_J64904
location: https://synbiohub.org/public/igem/BBa_J64904/annotation2313346/range2313346/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J64904/annotation2313346/range2313346/1
type: Range
persistent identity: range2313346
display id: range2313346
version: 1
top level: BBa_J64904
direction: direction/forward
start: 4743
end: 4783
orientation: inline

BBa_J64975
type: ComponentDefinition
persistent identity: BBa J64975
display id: BBa_J64975
version: 1
was generated by: iGEM to SBOL conversion
description: anti-glnG RNAi
created: 2007-03-23T12:00:00Z
modified: 2015-05-08T01:08:17Z
mutable provenance: ''E. coli'' K-12
owned by: james
owned by: myers
mutable description: Cognate RNAi for glnG from -40 to +24. Last 10 bases on 3' end are not involved in binding with glnG mRNA, but rather serve as a binding site for an additional RNAi
discontinued: false
dominant: false
group u list: _98_
m user id: 0
owner id: 1429
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Wanted an RNAi that would overlap the RBS and start codon for glnG. As an additional constraint on the design, also wanted to allow regulation of the cytosolic levels of this RNAi via an additional RNAi. This was accomplished by extending the 3' end with a G/C-rich region.
star: false
creator: Kristopher Kuchenbecker
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_J64975_sequence/1

glnG
type: ComponentDefinition
persistent identity: BBa J64977
display id: BBa_J64977
version: 1
was generated by: iGEM to SBOL conversion
description: glnG (ntrC)
created: 2007-03-23T12:00:00Z
modified: 2015-05-08T01:08:17Z
mutable provenance: ''E. coli'' K-12
owned by: james
owned by: myers
mutable description: In its phosphorylated state, NRI acts as a transcription enhancer for genes under the glnAp2 promoter.
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1429
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Sequence from K-12
star: false
creator: Kristopher Kuchenbecker
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_J64977_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

OmpR
type: ComponentDefinition
persistent identity: BBa R0082
display id: BBa_R0082
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (OmpR, positive)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: NC_000193 E. coli K12
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The promoter includes the three OmpR binding sites: C1, C2, and C3. The promoter starts at -107 and goes up to the transcriptional start site at +1. An alternate version, BBa_R0083, cuts out the C2 and C3 sites. The efficacy of this promoter versus the truncated ompC upstream region (BBa_R0083) or the ompF upstream region (BBa_R0084) is currently unknown.
star: true
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: C1 OmpR
sequence annotation: C2 OmpR
sequence annotation: C3 OmpR
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0082_sequence/1

C1 OmpR
type: SequenceAnnotation
persistent identity: annotation301154
display id: annotation301154
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
type: Range
persistent identity: range301154
display id: range301154
version: 1
top level: OmpR
direction: direction/forward
start: 13
end: 30
orientation: inline

C2 OmpR
type: SequenceAnnotation
persistent identity: annotation301155
display id: annotation301155
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
type: Range
persistent identity: range301155
display id: range301155
version: 1
top level: OmpR
direction: direction/forward
start: 34
end: 51
orientation: inline

C3 OmpR
type: SequenceAnnotation
persistent identity: annotation301156
display id: annotation301156
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
type: Range
persistent identity: range301156
display id: range301156
version: 1
top level: OmpR
direction: direction/forward
start: 54
end: 71
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301166
display id: annotation301166
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
type: Range
persistent identity: range301166
display id: range301166
version: 1
top level: OmpR
direction: direction/forward
start: 75
end: 80
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation301167
display id: annotation301167
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
type: Range
persistent identity: range301167
display id: range301167
version: 1
top level: OmpR
direction: direction/forward
start: 98
end: 103
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

BBa_F2620
type: ComponentDefinition
persistent identity: BBa F2620
display id: BBa_F2620
version: 1
was generated by: iGEM to SBOL conversion
description: 3OC6HSL -> PoPS Receiver
created: 2004-08-08T11:00:00Z
modified: 2015-08-31T04:07:27Z
mutable provenance: Formerly I13270
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This device senses the 3OC6HSL concentration in the media and produces output PoPS according to the transfer function given below. LuxR production is controlled by the tet repressible R0040. Initial characterization work has been done on this device using I13273 as a test device.
discontinued: false
dominant: true
experience: experience/Works
group u list: _101_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Example Data Sheets for F2620
star: true
creator: Barry Canton [bcanton@mit.edu] and Anna Labno [labnoa@mit.edu]
type: DnaRegion
role: SO:0000804
role: partType/Signalling
component: BBa_R0040
component: BBa_R0062
component: BBa_B0015
component: BBa_C0062
component: BBa_B0034
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_C0062
sequence annotation: BBa_B0015
sequence annotation: BBa_R0062
sequence: https://synbiohub.org/public/igem/BBa_F2620_sequence/1

BBa_R0040
type: Component
persistent identity: component2259889
display id: component2259889
version: 1
top level: BBa_F2620
definition: p(tetR)
access: public

BBa_R0062
type: Component
persistent identity: component2259910
display id: component2259910
version: 1
top level: BBa_F2620
definition: lux pR
access: public

BBa_B0015
type: Component
persistent identity: component2259908
display id: component2259908
version: 1
top level: BBa_F2620
definition: BBa_B0015
access: public

BBa_C0062
type: Component
persistent identity: component2259898
display id: component2259898
version: 1
top level: BBa_F2620
definition: luxr
access: public

BBa_B0034
type: Component
persistent identity: component2259895
display id: component2259895
version: 1
top level: BBa_F2620
definition: BBa_B0034
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2259889
display id: annotation2259889
version: 1
top level: BBa_F2620
location: https://synbiohub.org/public/igem/BBa_F2620/annotation2259889/range2259889/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_F2620/annotation2259889/range2259889/1
type: Range
persistent identity: range2259889
display id: range2259889
version: 1
top level: BBa_F2620
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2259895
display id: annotation2259895
version: 1
top level: BBa_F2620
location: https://synbiohub.org/public/igem/BBa_F2620/annotation2259895/range2259895/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_F2620/annotation2259895/range2259895/1
type: Range
persistent identity: range2259895
display id: range2259895
version: 1
top level: BBa_F2620
direction: direction/reverse
start: 63
end: 74
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2259898
display id: annotation2259898
version: 1
top level: BBa_F2620
location: https://synbiohub.org/public/igem/BBa_F2620/annotation2259898/range2259898/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_F2620/annotation2259898/range2259898/1
type: Range
persistent identity: range2259898
display id: range2259898
version: 1
top level: BBa_F2620
direction: direction/forward
start: 81
end: 836
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2259908
display id: annotation2259908
version: 1
top level: BBa_F2620
location: https://synbiohub.org/public/igem/BBa_F2620/annotation2259908/range2259908/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_F2620/annotation2259908/range2259908/1
type: Range
persistent identity: range2259908
display id: range2259908
version: 1
top level: BBa_F2620
direction: direction/reverse
start: 870
end: 998
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2259910
display id: annotation2259910
version: 1
top level: BBa_F2620
location: https://synbiohub.org/public/igem/BBa_F2620/annotation2259910/range2259910/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_F2620/annotation2259910/range2259910/1
type: Range
persistent identity: range2259910
display id: range2259910
version: 1
top level: BBa_F2620
direction: direction/forward
start: 1007
end: 1061
orientation: inline

BBa_I15008
type: ComponentDefinition
persistent identity: BBa I15008
display id: BBa_I15008
version: 1
was generated by: iGEM to SBOL conversion
description: heme oxygenase (ho1) from Synechocystis
created: 2004-09-19T11:00:00Z
modified: 2015-08-31T04:07:38Z
mutable provenance: Voigt Lab via Anselm Levskaya
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: One of two requisite genes required for the biosynthesis of phycocyanobilin from heme.
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
sample status: In stock
status: status/Available
bookmark: true
star: true
creator: Jeff Tabor
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I15008_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214026
display id: annotation2214026
version: 1
top level: BBa_I15008
location: https://synbiohub.org/public/igem/BBa_I15008/annotation2214026/range2214026/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_I15008/annotation2214026/range2214026/1
type: Range
persistent identity: range2214026
display id: range2214026
version: 1
top level: BBa_I15008
direction: direction/forward
start: 727
end: 751
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

BBa_I15009
type: ComponentDefinition
persistent identity: BBa I15009
display id: BBa_I15009
version: 1
was generated by: iGEM to SBOL conversion
description: phycocyanobilin:ferredoxin oxidoreductase (PcyA) from synechocystis
created: 2004-09-19T11:00:00Z
modified: 2015-08-31T04:07:38Z
mutable provenance: Voigt Lab via Anselm Levskaya
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Second of two required phycocyanobilin biosynthetic genes.
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
sample status: In stock
status: status/Available
bookmark: true
star: true
creator: Jeff Tabor
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PcyA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I15009_sequence/1

PcyA
type: SequenceAnnotation
persistent identity: annotation1891612
display id: annotation1891612
version: 1
top level: BBa_I15009
location: https://synbiohub.org/public/igem/BBa_I15009/annotation1891612/range1891612/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I15009/annotation1891612/range1891612/1
type: Range
persistent identity: range1891612
display id: range1891612
version: 1
top level: BBa_I15009
direction: direction/forward
start: 1
end: 750
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214032
display id: annotation2214032
version: 1
top level: BBa_I15009
location: https://synbiohub.org/public/igem/BBa_I15009/annotation2214032/range2214032/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_I15009/annotation2214032/range2214032/1
type: Range
persistent identity: range2214032
display id: range2214032
version: 1
top level: BBa_I15009
direction: direction/forward
start: 751
end: 775
orientation: inline

BBa_I15010
type: ComponentDefinition
persistent identity: BBa I15010
display id: BBa_I15010
version: 1
was generated by: iGEM to SBOL conversion
description: cph8 (Cph1/EnvZ fusion)
created: 2004-09-19T11:00:00Z
modified: 2015-08-31T04:07:38Z
mutable provenance: Voigt Lab via Anselm Levskaya
owned by: james
owned by: myers
mutable description: Chimeric Cph1 light receptor/EnvZ protein.
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Silent mutation at base 108 (G-A) to remove PstI site
star: false
creator: Jeff Tabor
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Cph8
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I15010_sequence/1

Cph8
type: SequenceAnnotation
persistent identity: annotation1891613
display id: annotation1891613
version: 1
top level: BBa_I15010
location: https://synbiohub.org/public/igem/BBa_I15010/annotation1891613/range1891613/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I15010/annotation1891613/range1891613/1
type: Range
persistent identity: range1891613
display id: range1891613
version: 1
top level: BBa_I15010
direction: direction/forward
start: 1
end: 2238
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214031
display id: annotation2214031
version: 1
top level: BBa_I15010
location: https://synbiohub.org/public/igem/BBa_I15010/annotation2214031/range2214031/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_I15010/annotation2214031/range2214031/1
type: Range
persistent identity: range2214031
display id: range2214031
version: 1
top level: BBa_I15010
direction: direction/forward
start: 2239
end: 2263
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

luxI
type: ComponentDefinition
persistent identity: BBa C0061
display id: BBa_C0061
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer synthetase for AHL
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Synthesizes 3OC6HSL, which binds to LuxR. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: An LVA tail (sequence: AANDENYALVA) was added to increase protein degradation. .
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0061
sequence annotation: luxI
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0061_sequence/1

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation7038
display id: annotation7038
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
type: Range
persistent identity: range7038
display id: range7038
version: 1
top level: luxI
direction: direction/forward
start: 1
end: 618
orientation: inline

luxI
type: SequenceAnnotation
persistent identity: annotation1761
display id: annotation1761
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
type: Range
persistent identity: range1761
display id: range1761
version: 1
top level: luxI
direction: direction/forward
start: 1
end: 579
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1760
display id: annotation1760
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
type: Range
persistent identity: range1760
display id: range1760
version: 1
top level: luxI
direction: direction/forward
start: 580
end: 611
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213985
display id: annotation2213985
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
type: Range
persistent identity: range2213985
display id: range2213985
version: 1
top level: luxI
direction: direction/forward
start: 619
end: 643
orientation: inline

lacI
type: ComponentDefinition
persistent identity: BBa C0012
display id: BBa_C0012
version: 1
was generated by: iGEM to SBOL conversion
description: lacI repressor from E. coli (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator <bb_part>BBa_R0010</bb_part> and PLlac01 hybrid regulator <bb_part>BBa_R0011</bb_part> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation. Incompatible with systems containing LacI, lactose, or IPTG.
star: true
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0012
sequence annotation: lacI-LVA
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0012_sequence/1

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation7031
display id: annotation7031
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
type: Range
persistent identity: range7031
display id: range7031
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

lacI-LVA
type: SequenceAnnotation
persistent identity: annotation1723
display id: annotation1723
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
type: Range
persistent identity: range1723
display id: range1723
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1722
display id: annotation1722
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
type: Range
persistent identity: range1722
display id: range1722
version: 1
top level: lacI
direction: direction/forward
start: 1090
end: 1128
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213988
display id: annotation2213988
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
type: Range
persistent identity: range2213988
display id: range2213988
version: 1
top level: lacI
direction: direction/forward
start: 1129
end: 1153
orientation: inline

BBa_E0044
type: ComponentDefinition
persistent identity: BBa E0044
display id: BBa_E0044
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212)
created: 2004-09-29T11:00:00Z
modified: 2015-08-31T04:07:25Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP-AAV [Pst1 site in earlier version knocked out]
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence: https://synbiohub.org/public/igem/BBa_E0044_sequence/1

glnAp2 pro
type: ComponentDefinition
persistent identity: BBa J64979
display id: BBa_J64979
version: 1
was generated by: iGEM to SBOL conversion
description: glnAp2
created: 2007-03-25T12:00:00Z
modified: 2015-05-08T01:08:18Z
mutable provenance: ''E. coli''
owned by: james
owned by: myers
mutable description: This promoter sequence (-80 to +1) is responsive to the transcription enhancer glnG.
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1429
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: n/a
star: false
creator: Kristopher Kuchenbecker
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J64979_sequence/1

BBa_J64931
type: ComponentDefinition
persistent identity: BBa J64931
display id: BBa_J64931
version: 1
was generated by: iGEM to SBOL conversion
description: glnKp promoter
created: 2007-03-25T11:00:00Z
modified: 2015-05-08T01:08:17Z
mutable provenance: ''E. coli'' K12
owned by: james
owned by: myers
mutable description: Natural promoter for glnK (-150 to 0)
discontinued: false
dominant: false
group u list: _98_
m user id: 0
owner id: 1429
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: n/a
star: false
creator: Kristopher Kuchenbecker
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J64931_sequence/1

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: iGEM to SBOL conversion
description: luxR repressor/activator, (no LVA?)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: luxR
sequence annotation: prefix
sequence annotation: BBa_C0062
sequence annotation: T
sequence annotation: A
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 750
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 2
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 756
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
top level: luxr
direction: direction/forward
start: 174
end: 174
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
top level: luxr
direction: direction/forward
start: 492
end: 492
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
top level: luxr
direction: direction/forward
start: 757
end: 781
orientation: inline

lux pR
type: ComponentDefinition
persistent identity: BBa R0062
display id: BBa_R0062
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (luxR & HSL regulated -- lux pR)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: V. fischeri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file>Image1.gif</bb_file>" width="614" height="362">
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0062
sequence annotation: LuxR/HSL
sequence annotation: -35
sequence annotation: -10
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0062_sequence/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation7070
display id: annotation7070
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
type: Range
persistent identity: range7070
display id: range7070
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 55
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2045
display id: annotation2045
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
type: Range
persistent identity: range2045
display id: range2045
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2046
display id: annotation2046
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
type: Range
persistent identity: range2046
display id: range2046
version: 1
top level: lux pR
direction: direction/forward
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2047
display id: annotation2047
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
type: Range
persistent identity: range2047
display id: range2047
version: 1
top level: lux pR
direction: direction/forward
start: 42
end: 47
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2048
display id: annotation2048
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
type: Range
persistent identity: range2048
display id: range2048
version: 1
top level: lux pR
direction: direction/forward
start: 53
end: 53
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

https://synbiohub.org/public/igem/BBa_J64994_sequence/1
type: Sequence
persistent identity: BBa J64994 sequence
display id: BBa_J64994_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagtcactccatccccagctcttttaacttacgcgtcagggtgttgcggccccagccgagtagccgcgccgcttcctgtttatgcccctgcgtatgtcgcaacgcggtcgtcagtaacgtccgctccagctctggctgcgcttcggaaagcagattttgatgaccggaacgcagcgctctgtctgcccactgcgcaagaagcgtcgcccagctgtccggttgcatttgcgaagtactctccgcaaccgttgattcaaacagttcgccgggcaaatcctgaatcaacacttcctgcccggcggccatcaccgttagccagcggcaggtgttttccagctggcgcacgttgcctggccacgccagacgcgtcagagcagcttcggtttccggatgcagtaacttcgcttctacgcccagttcgcgcgcggcaacctgtaaaaaatggcgcgccagacggggaatatcttcccgacgttcgcgcagcggcggcagatgaacgcggataacgttcaggcggtggaacagatcctcacggaacttaccttcctgcactcgctgttcgagattctggtgagtggcagcgataatccgcacatccactttcaccggcgcatagccgccaacgcggtaaaactgaccgtctgccagcacgcgcagcaaacgcgtctgcacatccagcggcatatcaccaatttcgtcgaggaataatgtaccgccatcggcctgttcaaaacgcccctgacgaatggtattcgcgccagtaaacgcgcctttctcgtggccaaacagttctgattcgatcaaatcttttgggatagctgccatattcagcgcgataaacggcgctttggcgcgcggactgtggcgatgcagggcatgagcgaccagttctttaccggtgccggattcgccgttaatcagcacgctaatagaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J64990_sequence/1
type: Sequence
persistent identity: BBa J64990 sequence
display id: BBa_J64990_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagtcactccatccccagctcttttaacttacgcgtcagggtgttgcggccccagccgagtagccgcgccgcttcctgtttatgcccctgcgtatgtcgcaacgcggtcgtcagtaacgtccgctccagctctggctgcgcttcggaaagcagattttgatgaccggaacgcagcgctctgtctgcccactgcgcaagaagcgtcgcccagctgtccggttgcatttgcgaagtactctccgcaaccgttgattcaaacagttcgccgggcaaatcctgaatcaacacttcctgcccggcggccatcaccgttagccagcggcaggtgttttccagctggcgcacgttgcctggccacgccagacgcgtcagagcagcttcggtttccggatgcagtaacttcgcttctacgcccagttcgcgcgcggcaacctgtaaaaaatggcgcgccagacggggaatatcttcccgacgttcgcgcagcggcggcagatgaacgcggataacgttcaggcggtggaacagatcctcacggaacttaccttcctgcactcgctgttcgagattctggtgagtggcagcgataatccgcacatccactttcaccggcgcatagccgccaacgcggtaaaactgaccgtctgccagcacgcgcagcaaacgcgtctgcacatccagcggcatatcaccaatttcgtcgaggaataatgtaccgccatcggcctgttcaaaacgcccctgacgaatggtattcgcgccagtaaacgcgcctttctcgtggccaaacagttctgattcgatcaaatcttttgggatagctgccatattcagcgcgataaacggcgctttggcgcgcggactgtggcgatgcagggcatgagcgaccagttctttaccggtgccggattcgccgttaatcagcacgctaatagaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J64903_sequence/1
type: Sequence
persistent identity: BBa J64903 sequence
display id: BBa_J64903_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctaataacgctgatagtgctagtgtagatcgctactagagattaaagaggagaaatactagatggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataataactctgatagtgctagtgtagatctctactagagattaaagaggagaaatactagatggccaccaccgtacaactcagcgaccaatccctccgtcagctagaaaccctcgccatccacaccgcccacctgattcagccccacggtttagtggtggtcctgcaagaaccagacctcaccatcagccaaattagcgccaactgcaccggcattttagggcgatcgccagaggatttgttgggcagaaccctaggggaagtgtttgatagctttcagattgatcccatccagagtcgcctaacggccggacaaatcagcagcctcaaccccagtaaactttgggcgcgggtcatgggggacgactttgtcatttttgacggggtttttcatcgcaacagtgacggtttat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J64904_sequence/1
type: Sequence
persistent identity: BBa J64904 sequence
display id: BBa_J64904_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagattgcaccaacatggtgcttaatgtttccattgaagcactatattggtgcaacattcacatcgtggtgcagcccttttgcacgatggtgcgcatgataacgccttttaggggcaatttaaaagttggcacagatttcgctttatctttttttactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagattaaagaggagaaatactagagtcactccatccccagctcttttaacttacgcgtcagggtgttgcggccccagccgagtagccgcgccgcttcctgtttatgcccctgcgtatgtcgcaacgcggtcgtcagtaacgtccgctccagctctggctgcgcttcggaaagcagattttgatgaccggaacgcagcgctctgtctgcccactgcgcaagaagcgtcgcccagctgtccggttgcatttgcgaagtactctccgcaaccgttgattcaaacagttcgccgggcaaatcctgaatcaacacttcctgcccggcggccatcaccgttagccagcggcaggtgttttccagctggcgcacgttgcctggccacgccagacgcgtcagagcagcttcggtttccggatgcagtaacttcgcttctacgcccagttcgcgcgcggcaacctgtaaaaaatggcgcgccagacggggaatatcttcccgacgttcgcgcagcggcggcagatgaacgcggataacgttcaggcggtggaacagatcctcacggaacttaccttcctgcactcgctgttcgagattctggtgagtggcagcgataatccgcacatccactttcaccggcgcatagccgccaacgcggtaaaactgaccgtctgccagcacgcgcagcaaacgcgtctgcacatccagcggcatatcaccaatttcgtcgaggaataatgtaccgccatcggcctgttcaaaacgcccctgacgaatggtattcgcgccagtaaacgcgcctttctcgtggccaaacagttctgattcgatcaaatcttttgggatagctgccatattcagcgcgataaacggcgctttggcgcgcggactgtggcgatgcagggcatgagcgaccagttctttaccggtgccggattcgccgttaatcagcacgctaatagaagaacgcgaaagccgaccgataatacggaacacgtcctgcatggctggcgcttcgccgatgatatcggtcgttgggccgttaagctgaacattacgcggctgctgctgttcctggtaatgactgatagcgcgctcaaccagcgccactgcttcgtcgatatcaaacggtttgggcagataatcaaacgccccttgttgataggcgctgacggcagcatccagatcggaatgtgcggtcataatgatgaccggaagcattggatggcgctgtttaatctgcttgagcagcgccagcccgtccattcccggcatacggatatctgaaagcagcacatccggcgttttgctcgccagcgcctccagcacttctgcgccgttctcaaacgtcgtacaggttaaacctgccccagcgagcgcacgttcaagcacccaacggatggaactatcgtcatcgactacccagactatccctcgttgcattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggtctgcactacgtcaacacgaatatgaaggataagagcgattatgacgctgacgcacgctatatcctgtccgtcaccaaatcgttttaattgtcataggcttccggccgcgggtggcgacaccctggccgggtaaca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J64975_sequence/1
type: Sequence
persistent identity: BBa J64975 sequence
display id: BBa_J64975_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttactgacgaccgcgttgcgacatacgcaggggcataaacaggaagcggcgcggctactcggctccccggcgcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J64977_sequence/1
type: Sequence
persistent identity: BBa J64977 sequence
display id: BBa_J64977_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcactccatccccagctcttttaacttacgcgtcagggtgttgcggccccagccgagtagccgcgccgcttcctgtttatgcccctgcgtatgtcgcaacgcggtcgtcagtaacgtccgctccagctctggctgcgcttcggaaagcagattttgatgaccggaacgcagcgctctgtctgcccactgcgcaagaagcgtcgcccagctgtccggttgcatttgcgaagtactctccgcaaccgttgattcaaacagttcgccgggcaaatcctgaatcaacacttcctgcccggcggccatcaccgttagccagcggcaggtgttttccagctggcgcacgttgcctggccacgccagacgcgtcagagcagcttcggtttccggatgcagtaacttcgcttctacgcccagttcgcgcgcggcaacctgtaaaaaatggcgcgccagacggggaatatcttcccgacgttcgcgcagcggcggcagatgaacgcggataacgttcaggcggtggaacagatcctcacggaacttaccttcctgcactcgctgttcgagattctggtgagtggcagcgataatccgcacatccactttcaccggcgcatagccgccaacgcggtaaaactgaccgtctgccagcacgcgcagcaaacgcgtctgcacatccagcggcatatcaccaatttcgtcgaggaataatgtaccgccatcggcctgttcaaaacgcccctgacgaatggtattcgcgccagtaaacgcgcctttctcgtggccaaacagttctgattcgatcaaatcttttgggatagctgccatattcagcgcgataaacggcgctttggcgcgcggactgtggcgatgcagggcatgagcgaccagttctttaccggtgccggattcgccgttaatcagcacgctaatagaagaacgcgaaagccgaccgataatacggaacacgtcctgcatggctggcgcttcgccgatgatatcggtcgttgggccgttaagctgaacattacgcggctgctgctgttcctggtaatgactgatagcgcgctcaaccagcgccactgcttcgtcgatatcaaacggtttgggcagataatcaaacgccccttgttgataggcgctgacggcagcatccagatcggaatgtgcggtcataatgatgaccggaagcattggatggcgctgtttaatctgcttgagcagcgccagcccgtccattcccggcatacggatatctgaaagcagcacatccggcgttttgctcgccagcgcctccagcacttctgcgccgttctcaaacgtcgtacaggttaaacctgccccagcgagcgcacgttcaagcacccaacggatggaactatcgtcatcgactacccagactatccctcgttgcat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0082_sequence/1
type: Sequence
persistent identity: BBa R0082 sequence
display id: BBa_R0082_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_F2620_sequence/1
type: Sequence
persistent identity: BBa F2620 sequence
display id: BBa_F2620_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I15008_sequence/1
type: Sequence
persistent identity: BBa I15008 sequence
display id: BBa_I15008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I15009_sequence/1
type: Sequence
persistent identity: BBa I15009 sequence
display id: BBa_I15009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I15010_sequence/1
type: Sequence
persistent identity: BBa I15010 sequence
display id: BBa_I15010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccaccaccgtacaactcagcgaccaatccctccgtcagctagaaaccctcgccatccacaccgcccacctgattcagccccacggtttagtggtggtcctgcaagaaccagacctcaccatcagccaaattagcgccaactgcaccggcattttagggcgatcgccagaggatttgttgggcagaaccctaggggaagtgtttgatagctttcagattgatcccatccagagtcgcctaacggccggacaaatcagcagcctcaaccccagtaaactttgggcgcgggtcatgggggacgactttgtcatttttgacggggtttttcatcgcaacagtgacggtttattggtatgtgaactcgagccagcctacacttccgataatctgcccttcctcggtttttatcacatggccaacgctgccctgaatcggttgcgccaacaagctaatctacgggatttctacgatgttattgtcgaagaagtccgccgtatgactggctttgaccgggtgatgctataccgctttgatgaaaataaccacggtgatgtcattgccgaagataaacgggatgatatggaaccctatttgggcctgcactatcccgaatcggatattccccaacccgcccgtcggctatttatccacaaccccattcgagtaattcccgatgtttatggtgtggcggtgcccctgaccccagcggttaaccccagcaccaaccgagcggtggatttaacagaatccattctgcgcagtgcgtaccattgccacttgacctatctgaaaaatatgggggtaggagcgtctttaaccatttccctaattaaggacggccatctctgggggctcattgcctgccaccatcaaacccccaaagtaattccctttgaactgcgtaaagcctgcgaattttttggtcgggtggtgtttagcaacatttccgcccaggaagatacggaaaccttcgattaccgggtgcagctggcggagcatgaagcggttttattggacaaaatgaccacggcggcggattttgtcgaaggattaactaatcatcccgatcgcctgttgggattaacgggctcccagggggcggccatttgctttggggaaaaattgattttagtaggggaaaccccggacgagaaagcagtgcaatatttactgcaatggttggagaatcgggaagtgcaagacgttttcttcacctcttccctctcacaaatttatcctgatgcagtgaattttaaatccgtggccagtggcttattggccattcccattgcccgtcacaactttttgctctggtttcgccctgaagtgttgcaaacggttaattggggcggtgacccaaatcatgcttacgaagctacccaggaagacggtaaaatcgagctccatccccgccaatcctttgacctctggaaagaaattgtccgactccaatctttgccctggcaatcggtggaaatccaaagtgccctggccctgaaaaaggcgatcgtcaacctcattttgcgccaggcagaagaattgcatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctgggtcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaaga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J64979_sequence/1
type: Sequence
persistent identity: BBa J64979 sequence
display id: BBa_J64979_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attgcaccaacatggtgcttaatgtttccattgaagcactatattggtgcaacattcacatcgtggtgcagcccttttgcacgatggtgcgcatgataacgccttttaggggcaatttaaaagttggcacagatttcgctttatctttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J64931_sequence/1
type: Sequence
persistent identity: BBa J64931 sequence
display id: BBa_J64931_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtctgcactacgtcaacacgaatatgaaggataagagcgattatgacgctgacgcacgctatatcctgtccgtcaccaaatcgttttaattgtcataggcttccggccgcgggtggcgacaccctggccgggtaacaggagaacccg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0061_sequence/1
type: Sequence
persistent identity: BBa C0061 sequence
display id: BBa_C0061_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0012_sequence/1
type: Sequence
persistent identity: BBa C0012 sequence
display id: BBa_C0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0044_sequence/1
type: Sequence
persistent identity: BBa E0044 sequence
display id: BBa_E0044_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctgctgctgtttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0062_sequence/1
type: Sequence
persistent identity: BBa C0062 sequence
display id: BBa_C0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0062_sequence/1
type: Sequence
persistent identity: BBa R0062 sequence
display id: BBa_R0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z