LOCUS       BBa_J69001              2564 bp    DNA     linear       26-Nov-2024
DEFINITION  LuxAIC with Terminated GFP output
ACCESSION   BBa_J69001
VERSION     BBa_J69001.1  
FEATURES             Location/Qualifiers
     stem_loop       1..129
                     /label=BBa_B0015
     misc_feature    131..136
                     /label=SphI restriction site
     CDS             138..893
                     /label=BBa_C0062 (luxR), codons optimised for E. coli
     RBS             900..911
                     /label=BBa_B0034
     misc_feature    913..918
                     /label=BamHI restriction site
     promoter        920..974
                     /label=BBa_R0011 (lac, lambda cI hybrid)
     misc_feature    976..981
                     /label=BspHI restriction site
     promoter        983..1037
                     /label=BBa_R0062 (lux pR)
     misc_feature    1039..1044
                     /label=SalI restriction site
     RBS             1046..1057
                     /label=BBa_B0034
     CDS             1064..1681
                     /label=BBa_C0061 (luxI), codons optimised for E. coli
     misc_feature    1..1681
                     /label=BBa_J28031
     misc_feature    1..1681
                     /label=BBa_J28031
     misc_feature    1694..1697
                     /label=conserved
     RBS             1690..1701
                     /label=BBa_B0034
     CDS             1708..2427
                     /label=GFP protein
     CDS             1708..2427
                     /label=BBa_E0040
     stem_loop       2447..2490
                     /label=stem_loop
     misc_feature    2436..2515
                     /label=BBa_B0010
     terminator      2436..2515
                     /label=BBa_B0010
     stem_loop       2531..2550
                     /label=T7 TE
     misc_feature    2557..2557
                     /label=stop
     polyA_site      2551..2564
                     /label=polya
     misc_feature    2524..2564
                     /label=BBa_B0012
     terminator      2524..2564
                     /label=BBa_B0012
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggt gcatgcgtta ttagtttttg aaatacgggc aatcgatcgc accggtcagg
      181 atagctttgc tgatagattg acaacgattc gtggtgttca gtttcatctg cgcgttggtc
      241 agatggaaag ttacggtgcg ttcagagcaa cccagaattt tggagatgtc ccaagaggac
      301 ttaccttcgc acgcccatgc caggcattct ttctcgcgtt tggtcaggtc gttgttgctt
      361 ttgttattgg caatgttaat cttgcgatag ttatcaacca gggacggaac gatcagagga
      421 atgttcatgc acgcgtgcag gaacagagaa tcgatgtagt tgtctttttc ggagtgcgca
      481 aaagacagca taccgaagcc gttgttcgcg gtatgaatcg ggaagctgaa accggtgatc
      541 agaccgctgg tttttgcttc tttgataacg tttggggact ttttgttaac cgcgttgttc
      601 tcaaagatat tccagttaat cggggaatgg ttagagttag agtaatctac gatcggatcg
      661 tacttgatca ggttggcgtc atcatagtac tgacgccatt tttttgggta gttatccaga
      721 atagaaatat cggattttac catgctgtgc ggataaatga tggccagcag ataatattca
      781 cagtgaacca ttttggtcat atcagacagg cattggttaa tatcgttgtt agaacgacac
      841 gctttgattt tgttaatgat acggtaggtg tcgtccgcgt tgatgttttt catctagtat
      901 ttctcctctt ttggatccgt gtgctcagta tcttgttatc cgctcacaat gtcaattgtt
      961 atccgctcac aattttcatg agacctgtag gatcgtacag gtttacgcaa gaaaatggtt
     1021 tgttatagtc gaataaatgt cgacgaaaga ggagaaatac tagatgacca ttatgatcaa
     1081 gaaatctgac ttcctggcca tcccgtccga agaatacaaa ggcatcctgt ctctgcgcta
     1141 ccaggttttc aaacagcgtc tggaatggga cctggtggtt gaaaacaacc tggaatctga
     1201 cgagtatgat aattccaacg cagaatacat ctatgcgtgt gatgatactg aaaacgtttc
     1261 tggttgttgg cgtctgctgc ctaccacggg cgattatatg ctgaagtccg tatttccgga
     1321 gctgctgggt cagcagtccg cgccgaaaga cccgaacatt gtagaactgt ctcgcttcgc
     1381 ggttggcaag aacagctcta aaatcaacaa ctccgcaagc gaaattacca tgaaactgtt
     1441 cgaagctatt tataaacacg cggtttccca gggtatcacc gaatacgtga ccgtcactag
     1501 caccgctatc gagcgcttcc tgaaacgtat caaggttccg tgccaccgta tcggtgacaa
     1561 agaaatccac gtactgggtg ataccaagag cgtcgtgctg tccatgccga ttaacgagca
     1621 gtttaagaaa gctgtgctga acgccgctaa cgacgaaaac tacgcgctgg tggcgtaata
     1681 atactagaga aagaggagaa atactagatg cgtaaaggag aagaactttt cactggagtt
     1741 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga
     1801 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga
     1861 aaactacctg ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt
     1921 gcgagatacc cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt
     1981 tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa
     2041 gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa
     2101 gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac
     2161 atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt
     2221 gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
     2281 cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc
     2341 aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat
     2401 ggcatggatg aactatacaa ataataatac tagagccagg catcaaataa aacgaaaggc
     2461 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     2521 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//