##gff-version 3
##sequence-region BBa_J69004 1 2413
BBa_J69004	.	CDS	1708	2413	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_J69004	.	engineered_tag	2389	2413	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_J69004	.	CDS	1708	2382	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_J69004	.	engineered_region	1	1681	.	+	0	ID=BBa_J28031;Name=BBa_J28031
BBa_J69004	.	sequence_feature	131	136	.	+	0	ID=annotation1904927;Name=SphI restriction site;Parent=BBa_J28031
BBa_J69004	.	engineered_region	1	1681	.	+	0	ID=annotation1904937;Name=BBa_J28031;Parent=BBa_J28031
BBa_J69004	.	stem_loop	1	129	.	+	0	ID=annotation1904926;Name=BBa_B0015;Parent=BBa_J28031
BBa_J69004	.	CDS	1064	1681	.	+	0	ID=annotation1904936;Name=BBa_C0061 (luxI), codons optimised for E. coli;Parent=BBa_J28031
BBa_J69004	.	sequence_feature	913	918	.	+	0	ID=annotation1904930;Name=BamHI restriction site;Parent=BBa_J28031
BBa_J69004	.	sequence_feature	976	981	.	+	0	ID=annotation1904932;Name=BspHI restriction site;Parent=BBa_J28031
BBa_J69004	.	sequence_feature	1039	1044	.	+	0	ID=annotation1904934;Name=SalI restriction site;Parent=BBa_J28031
BBa_J69004	.	ribosome_entry_site	900	911	.	+	0	ID=annotation1904929;Name=BBa_B0034;Parent=BBa_J28031
BBa_J69004	.	CDS	138	893	.	+	0	ID=annotation1904928;Name=BBa_C0062 (luxR), codons optimised for E. coli;Parent=BBa_J28031
BBa_J69004	.	ribosome_entry_site	1046	1057	.	+	0	ID=annotation1904935;Name=BBa_B0034;Parent=BBa_J28031
BBa_J69004	.	promoter	920	974	.	+	0	ID=annotation1904931;Name=BBa_R0011 (lac, lambda cI hybrid);Parent=BBa_J28031
BBa_J69004	.	promoter	983	1037	.	+	0	ID=annotation1904933;Name=BBa_R0062 (lux pR);Parent=BBa_J28031
BBa_J69004	.	ribosome_entry_site	1690	1701	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J69004	.	conserved_region	1694	1697	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_J69004
tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaa
acgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggtgcatgcgttattagtttttgaaatacgg
gcaatcgatcgcaccggtcaggatagctttgctgatagattgacaacgattcgtggtgttcagtttcatctgcgcgttg
gtcagatggaaagttacggtgcgttcagagcaacccagaattttggagatgtcccaagaggacttaccttcgcacgccc
atgccaggcattctttctcgcgtttggtcaggtcgttgttgcttttgttattggcaatgttaatcttgcgatagttatc
aaccagggacggaacgatcagaggaatgttcatgcacgcgtgcaggaacagagaatcgatgtagttgtctttttcggag
tgcgcaaaagacagcataccgaagccgttgttcgcggtatgaatcgggaagctgaaaccggtgatcagaccgctggttt
ttgcttctttgataacgtttggggactttttgttaaccgcgttgttctcaaagatattccagttaatcggggaatggtt
agagttagagtaatctacgatcggatcgtacttgatcaggttggcgtcatcatagtactgacgccatttttttgggtag
ttatccagaatagaaatatcggattttaccatgctgtgcggataaatgatggccagcagataatattcacagtgaacca
ttttggtcatatcagacaggcattggttaatatcgttgttagaacgacacgctttgattttgttaatgatacggtaggt
gtcgtccgcgttgatgtttttcatctagtatttctcctcttttggatccgtgtgctcagtatcttgttatccgctcaca
atgtcaattgttatccgctcacaattttcatgagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttata
gtcgaataaatgtcgacgaaagaggagaaatactagatgaccattatgatcaagaaatctgacttcctggccatcccgt
ccgaagaatacaaaggcatcctgtctctgcgctaccaggttttcaaacagcgtctggaatgggacctggtggttgaaaa
caacctggaatctgacgagtatgataattccaacgcagaatacatctatgcgtgtgatgatactgaaaacgtttctggt
tgttggcgtctgctgcctaccacgggcgattatatgctgaagtccgtatttccggagctgctgggtcagcagtccgcgc
cgaaagacccgaacattgtagaactgtctcgcttcgcggttggcaagaacagctctaaaatcaacaactccgcaagcga
aattaccatgaaactgttcgaagctatttataaacacgcggtttcccagggtatcaccgaatacgtgaccgtcactagc
accgctatcgagcgcttcctgaaacgtatcaaggttccgtgccaccgtatcggtgacaaagaaatccacgtactgggtg
ataccaagagcgtcgtgctgtccatgccgattaacgagcagtttaagaaagctgtgctgaacgccgctaacgacgaaaa
ctacgcgctggtggcgtaataatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagt
tcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtcc
gtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccg
cagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaag
gtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacgg
tgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggt
tgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaag
acggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaac
cgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcac
tccaccggtgcttaataacgctgatagtgctagtgtagatcgc