LOCUS       BBa_J69006              2421 bp    DNA     linear       22-Nov-2024
DEFINITION  LuxAIC with mCherry output
ACCESSION   BBa_J69006
VERSION     BBa_J69006.1  
FEATURES             Location/Qualifiers
     stem_loop       1..129
                     /label=BBa_B0015
     misc_feature    131..136
                     /label=SphI restriction site
     CDS             138..893
                     /label=BBa_C0062 (luxR), codons optimised for E. coli
     RBS             900..911
                     /label=BBa_B0034
     misc_feature    913..918
                     /label=BamHI restriction site
     promoter        920..974
                     /label=BBa_R0011 (lac, lambda cI hybrid)
     misc_feature    976..981
                     /label=BspHI restriction site
     promoter        983..1037
                     /label=BBa_R0062 (lux pR)
     misc_feature    1039..1044
                     /label=SalI restriction site
     RBS             1046..1057
                     /label=BBa_B0034
     CDS             1064..1681
                     /label=BBa_C0061 (luxI), codons optimised for E. coli
     misc_feature    1..1681
                     /label=BBa_J28031
     misc_feature    1..1681
                     /label=BBa_J28031
     misc_feature    1694..1697
                     /label=conserved
     RBS             1690..1701
                     /label=BBa_B0034
     CDS             1708..2418
                     /label=mCherry
     misc_feature    2059..2059
                     /label=C->T (removing PstI site)
     misc_feature    1708..2421
                     /label=BBa_J06504
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggt gcatgcgtta ttagtttttg aaatacgggc aatcgatcgc accggtcagg
      181 atagctttgc tgatagattg acaacgattc gtggtgttca gtttcatctg cgcgttggtc
      241 agatggaaag ttacggtgcg ttcagagcaa cccagaattt tggagatgtc ccaagaggac
      301 ttaccttcgc acgcccatgc caggcattct ttctcgcgtt tggtcaggtc gttgttgctt
      361 ttgttattgg caatgttaat cttgcgatag ttatcaacca gggacggaac gatcagagga
      421 atgttcatgc acgcgtgcag gaacagagaa tcgatgtagt tgtctttttc ggagtgcgca
      481 aaagacagca taccgaagcc gttgttcgcg gtatgaatcg ggaagctgaa accggtgatc
      541 agaccgctgg tttttgcttc tttgataacg tttggggact ttttgttaac cgcgttgttc
      601 tcaaagatat tccagttaat cggggaatgg ttagagttag agtaatctac gatcggatcg
      661 tacttgatca ggttggcgtc atcatagtac tgacgccatt tttttgggta gttatccaga
      721 atagaaatat cggattttac catgctgtgc ggataaatga tggccagcag ataatattca
      781 cagtgaacca ttttggtcat atcagacagg cattggttaa tatcgttgtt agaacgacac
      841 gctttgattt tgttaatgat acggtaggtg tcgtccgcgt tgatgttttt catctagtat
      901 ttctcctctt ttggatccgt gtgctcagta tcttgttatc cgctcacaat gtcaattgtt
      961 atccgctcac aattttcatg agacctgtag gatcgtacag gtttacgcaa gaaaatggtt
     1021 tgttatagtc gaataaatgt cgacgaaaga ggagaaatac tagatgacca ttatgatcaa
     1081 gaaatctgac ttcctggcca tcccgtccga agaatacaaa ggcatcctgt ctctgcgcta
     1141 ccaggttttc aaacagcgtc tggaatggga cctggtggtt gaaaacaacc tggaatctga
     1201 cgagtatgat aattccaacg cagaatacat ctatgcgtgt gatgatactg aaaacgtttc
     1261 tggttgttgg cgtctgctgc ctaccacggg cgattatatg ctgaagtccg tatttccgga
     1321 gctgctgggt cagcagtccg cgccgaaaga cccgaacatt gtagaactgt ctcgcttcgc
     1381 ggttggcaag aacagctcta aaatcaacaa ctccgcaagc gaaattacca tgaaactgtt
     1441 cgaagctatt tataaacacg cggtttccca gggtatcacc gaatacgtga ccgtcactag
     1501 caccgctatc gagcgcttcc tgaaacgtat caaggttccg tgccaccgta tcggtgacaa
     1561 agaaatccac gtactgggtg ataccaagag cgtcgtgctg tccatgccga ttaacgagca
     1621 gtttaagaaa gctgtgctga acgccgctaa cgacgaaaac tacgcgctgg tggcgtaata
     1681 atactagaga aagaggagaa atactagatg gtgagcaagg gcgaggagga taacatggcc
     1741 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     1801 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     1861 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     1921 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
     1981 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     2041 gtgacccagg actcctcctt gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     2101 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     2161 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     2221 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
     2281 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     2341 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
     2401 gacgagctgt acaagtaata a
//