LOCUS       BBa_J69011              1920 bp    DNA     linear       22-Dec-2024
DEFINITION  3OC6-HSL Lux Reciever Device (Codon-Optimised) with GFP Reporter
ACCESSION   BBa_J69011
VERSION     BBa_J69011.1  
FEATURES             Location/Qualifiers
     misc_feature    1..129
                     /label=BBa_B0015
     misc_feature    131..136
                     /label=SphI
     misc_feature    138..893
                     /label=C0362 luxR (codon-optimised)
     RBS             900..911
                     /label=B0034
     misc_feature    913..918
                     /label=BamHI
     promoter        920..974
                     /label=R0011 - lac lamda CI hybrid
     misc_feature    976..981
                     /label=BspH
     promoter        983..1037
                     /label=R0062 - lux pR
     misc_feature    1..1037
                     /label=BBa_J69010
     misc_feature    1050..1053
                     /label=conserved
     RBS             1046..1057
                     /label=BBa_B0034
     CDS             1064..1783
                     /label=GFP protein
     CDS             1064..1783
                     /label=BBa_E0040
     stem_loop       1803..1846
                     /label=stem_loop
     misc_feature    1792..1871
                     /label=BBa_B0010
     terminator      1792..1871
                     /label=BBa_B0010
     stem_loop       1887..1906
                     /label=T7 TE
     misc_feature    1913..1913
                     /label=stop
     polyA_site      1907..1920
                     /label=polya
     misc_feature    1880..1920
                     /label=BBa_B0012
     terminator      1880..1920
                     /label=BBa_B0012
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggt gcatgcgtta ttagtttttg aaatacgggc aatcgatcgc accggtcagg
      181 atagctttgc tgatagattg acaacgattc gtggtgttca gtttcatctg cgcgttggtc
      241 agatggaaag ttacggtgcg ttcagagcaa cccagaattt tggagatgtc ccaagaggac
      301 ttaccttcgc acgcccatgc caggcattct ttctcgcgtt tggtcaggtc gttgttgctt
      361 ttgttattgg caatgttaat cttgcgatag ttatcaacca gggacggaac gatcagagga
      421 atgttcatgc acgcgtgcag gaacagagaa tcgatgtagt tgtctttttc ggagtgcgca
      481 aaagacagca taccgaagcc gttgttcgcg gtatgaatcg ggaagctgaa accggtgatc
      541 agaccgctgg tttttgcttc tttgataacg tttggggact ttttgttaac cgcgttgttc
      601 tcaaagatat tccagttaat cggggaatgg ttagagttag agtaatctac gatcggatcg
      661 tacttgatca ggttggcgtc atcatagtac tgacgccatt tttttgggta gttatccaga
      721 atagaaatat cggattttac catgctgtgc ggataaatga tggccagcag ataatattca
      781 cagtgaacca ttttggtcat atcagacagg cattggttaa tatcgttgtt agaacgacac
      841 gctttgattt tgttaatgat acggtaggtg tcgtccgcgt tgatgttttt catctagtat
      901 ttctcctctt ttggatccgt gtgctcagta tcttgttatc cgctcacaat gtcaattgtt
      961 atccgctcac aattttcatg agacctgtag gatcgtacag gtttacgcaa gaaaatggtt
     1021 tgttatagtc gaataaatac tagagaaaga ggagaaatac tagatgcgta aaggagaaga
     1081 acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa
     1141 attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt
     1201 tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcggtta
     1261 tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag
     1321 tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta
     1381 caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa
     1441 aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa
     1501 ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa
     1561 aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac
     1621 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc
     1681 cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc
     1741 tgctgggatt acacatggca tggatgaact atacaaataa taatactaga gccaggcatc
     1801 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     1861 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//