LOCUS       BBa_J69020              2624 bp    DNA     linear       26-Nov-2024
DEFINITION  Lux Auto-inducible Device 1
ACCESSION   BBa_J69020
VERSION     BBa_J69020.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             76..798
                     /label=BBa_E0030
     stem_loop       818..861
                     /label=stem_loop
     misc_feature    807..886
                     /label=BBa_B0010
     terminator      807..886
                     /label=BBa_B0010
     stem_loop       902..921
                     /label=T7 TE
     misc_feature    928..928
                     /label=stop
     polyA_site      922..935
                     /label=polya
     misc_feature    895..935
                     /label=BBa_B0012
     terminator      895..935
                     /label=BBa_B0012
     terminator      807..935
                     /label=BBa_B0015
     misc_feature    1..935
                     /label=BBa_J69504
     stem_loop       944..1072
                     /label=BBa_B0015
     misc_feature    1074..1079
                     /label=SphI restriction site
     CDS             1081..1836
                     /label=BBa_C0062 (luxR), codons optimised for E. coli
     RBS             1843..1854
                     /label=BBa_B0034
     misc_feature    1856..1861
                     /label=BamHI restriction site
     promoter        1863..1917
                     /label=BBa_R0011 (lac, lambda cI hybrid)
     misc_feature    1919..1924
                     /label=BspHI restriction site
     promoter        1926..1980
                     /label=BBa_R0062 (lux pR)
     misc_feature    1982..1987
                     /label=SalI restriction site
     RBS             1989..2000
                     /label=BBa_B0034
     CDS             2007..2624
                     /label=BBa_C0061 (luxI), codons optimised for E. coli
     misc_feature    944..2624
                     /label=BBa_J28031
     misc_feature    944..2624
                     /label=BBa_J28031
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa
       61 gaggagaaat actagatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
      121 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
      181 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
      241 gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcaatgctt cgcccgctac
      301 cccgaccaca tgaagctgca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
      361 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
      421 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
      481 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
      541 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
      601 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
      661 ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag
      721 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
      781 gagctgtaca agtaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
      841 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
      901 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagtataaac gcagaaaggc
      961 ccacccgaag gtgagccagt gtgactctag tagagagcgt tcaccgacaa acaacagata
     1021 aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttgatgcct ggtgcatgcg
     1081 ttattagttt ttgaaatacg ggcaatcgat cgcaccggtc aggatagctt tgctgataga
     1141 ttgacaacga ttcgtggtgt tcagtttcat ctgcgcgttg gtcagatgga aagttacggt
     1201 gcgttcagag caacccagaa ttttggagat gtcccaagag gacttacctt cgcacgccca
     1261 tgccaggcat tctttctcgc gtttggtcag gtcgttgttg cttttgttat tggcaatgtt
     1321 aatcttgcga tagttatcaa ccagggacgg aacgatcaga ggaatgttca tgcacgcgtg
     1381 caggaacaga gaatcgatgt agttgtcttt ttcggagtgc gcaaaagaca gcataccgaa
     1441 gccgttgttc gcggtatgaa tcgggaagct gaaaccggtg atcagaccgc tggtttttgc
     1501 ttctttgata acgtttgggg actttttgtt aaccgcgttg ttctcaaaga tattccagtt
     1561 aatcggggaa tggttagagt tagagtaatc tacgatcgga tcgtacttga tcaggttggc
     1621 gtcatcatag tactgacgcc atttttttgg gtagttatcc agaatagaaa tatcggattt
     1681 taccatgctg tgcggataaa tgatggccag cagataatat tcacagtgaa ccattttggt
     1741 catatcagac aggcattggt taatatcgtt gttagaacga cacgctttga ttttgttaat
     1801 gatacggtag gtgtcgtccg cgttgatgtt tttcatctag tatttctcct cttttggatc
     1861 cgtgtgctca gtatcttgtt atccgctcac aatgtcaatt gttatccgct cacaattttc
     1921 atgagacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataaa
     1981 tgtcgacgaa agaggagaaa tactagatga ccattatgat caagaaatct gacttcctgg
     2041 ccatcccgtc cgaagaatac aaaggcatcc tgtctctgcg ctaccaggtt ttcaaacagc
     2101 gtctggaatg ggacctggtg gttgaaaaca acctggaatc tgacgagtat gataattcca
     2161 acgcagaata catctatgcg tgtgatgata ctgaaaacgt ttctggttgt tggcgtctgc
     2221 tgcctaccac gggcgattat atgctgaagt ccgtatttcc ggagctgctg ggtcagcagt
     2281 ccgcgccgaa agacccgaac attgtagaac tgtctcgctt cgcggttggc aagaacagct
     2341 ctaaaatcaa caactccgca agcgaaatta ccatgaaact gttcgaagct atttataaac
     2401 acgcggtttc ccagggtatc accgaatacg tgaccgtcac tagcaccgct atcgagcgct
     2461 tcctgaaacg tatcaaggtt ccgtgccacc gtatcggtga caaagaaatc cacgtactgg
     2521 gtgataccaa gagcgtcgtg ctgtccatgc cgattaacga gcagtttaag aaagctgtgc
     2581 tgaacgccgc taacgacgaa aactacgcgc tggtggcgta ataa
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