##gff-version 3
##sequence-region BBa_J69020 1 2624
BBa_J69020	.	engineered_region	1	935	.	+	0	ID=BBa_J69504;Name=BBa_J69504
BBa_J69020	.	ribosome_entry_site	58	69	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J69504
BBa_J69020	.	conserved_region	62	65	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J69020	.	promoter	1	49	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_J69504
BBa_J69020	.	engineered_region	1	49	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_J69020	.	non_covalent_binding_site	25	41	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_J69020	.	promoter	15	20	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_J69020	.	non_covalent_binding_site	1	17	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_J69020	.	promoter	38	43	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_J69020	.	CDS	76	798	.	+	0	ID=BBa_E0030;Name=BBa_E0030;Parent=BBa_J69504
BBa_J69020	.	terminator	807	935	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_J69504
BBa_J69020	.	terminator	895	935	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J69020	.	polyA_site	922	935	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J69020	.	stem_loop	902	921	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J69020	.	stop_codon	928	928	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J69020	.	engineered_region	895	935	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J69020	.	terminator	807	886	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J69020	.	stem_loop	818	861	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J69020	.	engineered_region	807	886	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J69020	.	engineered_region	944	2624	.	+	0	ID=BBa_J28031;Name=BBa_J28031
BBa_J69020	.	stem_loop	944	1072	.	+	0	ID=annotation1904926;Name=BBa_B0015;Parent=BBa_J28031
BBa_J69020	.	sequence_feature	1856	1861	.	+	0	ID=annotation1904930;Name=BamHI restriction site;Parent=BBa_J28031
BBa_J69020	.	sequence_feature	1982	1987	.	+	0	ID=annotation1904934;Name=SalI restriction site;Parent=BBa_J28031
BBa_J69020	.	ribosome_entry_site	1989	2000	.	+	0	ID=annotation1904935;Name=BBa_B0034;Parent=BBa_J28031
BBa_J69020	.	CDS	2007	2624	.	+	0	ID=annotation1904936;Name=BBa_C0061 (luxI), codons optimised for E. coli;Parent=BBa_J28031
BBa_J69020	.	sequence_feature	1074	1079	.	+	0	ID=annotation1904927;Name=SphI restriction site;Parent=BBa_J28031
BBa_J69020	.	engineered_region	944	2624	.	+	0	ID=annotation1904937;Name=BBa_J28031;Parent=BBa_J28031
BBa_J69020	.	sequence_feature	1919	1924	.	+	0	ID=annotation1904932;Name=BspHI restriction site;Parent=BBa_J28031
BBa_J69020	.	ribosome_entry_site	1843	1854	.	+	0	ID=annotation1904929;Name=BBa_B0034;Parent=BBa_J28031
BBa_J69020	.	promoter	1863	1917	.	+	0	ID=annotation1904931;Name=BBa_R0011 (lac, lambda cI hybrid);Parent=BBa_J28031
BBa_J69020	.	CDS	1081	1836	.	+	0	ID=annotation1904928;Name=BBa_C0062 (luxR), codons optimised for E. coli;Parent=BBa_J28031
BBa_J69020	.	promoter	1926	1980	.	+	0	ID=annotation1904933;Name=BBa_R0062 (lux pR);Parent=BBa_J28031
>BBa_J69020
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatgg
tgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagtt
cagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctg
cccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagc
tgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaacta
caagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggag
gacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaaga
acggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaa
cacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagacccc
aacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtaca
agtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtt
tgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtataa
acgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaa
aggcccagtctttcgactgagcctttcgttttatttgatgcctggtgcatgcgttattagtttttgaaatacgggcaat
cgatcgcaccggtcaggatagctttgctgatagattgacaacgattcgtggtgttcagtttcatctgcgcgttggtcag
atggaaagttacggtgcgttcagagcaacccagaattttggagatgtcccaagaggacttaccttcgcacgcccatgcc
aggcattctttctcgcgtttggtcaggtcgttgttgcttttgttattggcaatgttaatcttgcgatagttatcaacca
gggacggaacgatcagaggaatgttcatgcacgcgtgcaggaacagagaatcgatgtagttgtctttttcggagtgcgc
aaaagacagcataccgaagccgttgttcgcggtatgaatcgggaagctgaaaccggtgatcagaccgctggtttttgct
tctttgataacgtttggggactttttgttaaccgcgttgttctcaaagatattccagttaatcggggaatggttagagt
tagagtaatctacgatcggatcgtacttgatcaggttggcgtcatcatagtactgacgccatttttttgggtagttatc
cagaatagaaatatcggattttaccatgctgtgcggataaatgatggccagcagataatattcacagtgaaccattttg
gtcatatcagacaggcattggttaatatcgttgttagaacgacacgctttgattttgttaatgatacggtaggtgtcgt
ccgcgttgatgtttttcatctagtatttctcctcttttggatccgtgtgctcagtatcttgttatccgctcacaatgtc
aattgttatccgctcacaattttcatgagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcga
ataaatgtcgacgaaagaggagaaatactagatgaccattatgatcaagaaatctgacttcctggccatcccgtccgaa
gaatacaaaggcatcctgtctctgcgctaccaggttttcaaacagcgtctggaatgggacctggtggttgaaaacaacc
tggaatctgacgagtatgataattccaacgcagaatacatctatgcgtgtgatgatactgaaaacgtttctggttgttg
gcgtctgctgcctaccacgggcgattatatgctgaagtccgtatttccggagctgctgggtcagcagtccgcgccgaaa
gacccgaacattgtagaactgtctcgcttcgcggttggcaagaacagctctaaaatcaacaactccgcaagcgaaatta
ccatgaaactgttcgaagctatttataaacacgcggtttcccagggtatcaccgaatacgtgaccgtcactagcaccgc
tatcgagcgcttcctgaaacgtatcaaggttccgtgccaccgtatcggtgacaaagaaatccacgtactgggtgatacc
aagagcgtcgtgctgtccatgccgattaacgagcagtttaagaaagctgtgctgaacgccgctaacgacgaaaactacg
cgctggtggcgtaataa