BBa_J69020
type: ComponentDefinition
persistent identity: BBa J69020
display id: BBa_J69020
version: 1
was generated by: iGEM to SBOL conversion
description: Lux Auto-inducible Device 1
created: 2008-09-22T11:00:00Z
modified: 2015-05-08T01:08:18Z
mutable provenance: NA
owned by: james
owned by: myers
mutable description: NA
discontinued: false
dominant: false
group u list: _101_
m user id: 0
owner id: 740
owning group id: 101
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: NA
star: false
creator: James Brown
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_J28031
component: BBa_J69504
sequence annotation: BBa_J69504
sequence annotation: BBa_J28031
sequence: https://synbiohub.org/public/igem/BBa_J69020_sequence/1

BBa_J28031
type: Component
persistent identity: component2224043
display id: component2224043
version: 1
top level: BBa_J69020
definition: BBa_J28031
access: public

BBa_J69504
type: Component
persistent identity: component2224042
display id: component2224042
version: 1
top level: BBa_J69020
definition: BBa_J69504
access: public

BBa_J69504
type: SequenceAnnotation
persistent identity: annotation2224042
display id: annotation2224042
version: 1
top level: BBa_J69020
location: https://synbiohub.org/public/igem/BBa_J69020/annotation2224042/range2224042/1
component: BBa_J69504

https://synbiohub.org/public/igem/BBa_J69020/annotation2224042/range2224042/1
type: Range
persistent identity: range2224042
display id: range2224042
version: 1
top level: BBa_J69020
direction: direction/reverse
start: 1
end: 935
orientation: inline

BBa_J28031
type: SequenceAnnotation
persistent identity: annotation2224043
display id: annotation2224043
version: 1
top level: BBa_J69020
location: https://synbiohub.org/public/igem/BBa_J69020/annotation2224043/range2224043/1
component: BBa_J28031

https://synbiohub.org/public/igem/BBa_J69020/annotation2224043/range2224043/1
type: Range
persistent identity: range2224043
display id: range2224043
version: 1
top level: BBa_J69020
direction: direction/reverse
start: 944
end: 2624
orientation: inline

BBa_J28031
type: ComponentDefinition
persistent identity: BBa J28031
display id: BBa_J28031
version: 1
was generated by: iGEM to SBOL conversion
description: lux autoinducer
created: 2006-10-23T11:00:00Z
modified: 2015-08-31T04:08:44Z
mutable provenance: soon
owned by: james
owned by: myers
mutable description: soon
discontinued: false
dominant: false
experience: experience/None
group u list: _101_
m user id: 0
owner id: 1072
owning group id: 57
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: soon
star: true
creator: Cambridge iGEM 2006 Team
type: DnaRegion
role: SO:0000804
role: partType/Signalling
sequence annotation: BBa_B0015
sequence annotation: BBa_J28031
sequence annotation: SphI restriction site
sequence annotation: BBa_C0062 (luxR), codons optimised for E. coli
sequence annotation: BBa_B0034
sequence annotation: BamHI restriction site
sequence annotation: BBa_R0011 (lac, lambda cI hybrid)
sequence annotation: BspHI restriction site
sequence annotation: BBa_R0062 (lux pR)
sequence annotation: SalI restriction site
sequence annotation: BBa_C0061 (luxI), codons optimised for E. coli
sequence: https://synbiohub.org/public/igem/BBa_J28031_sequence/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation1904926
display id: annotation1904926
version: 1
top level: BBa_J28031
location: https://synbiohub.org/public/igem/BBa_J28031/annotation1904926/range1904926/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_J28031/annotation1904926/range1904926/1
type: Range
persistent identity: range1904926
display id: range1904926
version: 1
top level: BBa_J28031
direction: direction/reverse
start: 1
end: 129
orientation: inline

BBa_J28031
type: SequenceAnnotation
persistent identity: annotation1904937
display id: annotation1904937
version: 1
top level: BBa_J28031
location: https://synbiohub.org/public/igem/BBa_J28031/annotation1904937/range1904937/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_J28031/annotation1904937/range1904937/1
type: Range
persistent identity: range1904937
display id: range1904937
version: 1
top level: BBa_J28031
direction: direction/reverse
start: 1
end: 1681
orientation: inline

SphI restriction site
type: SequenceAnnotation
persistent identity: annotation1904927
display id: annotation1904927
version: 1
top level: BBa_J28031
location: https://synbiohub.org/public/igem/BBa_J28031/annotation1904927/range1904927/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J28031/annotation1904927/range1904927/1
type: Range
persistent identity: range1904927
display id: range1904927
version: 1
top level: BBa_J28031
direction: direction/forward
start: 131
end: 136
orientation: inline

BBa_C0062 (luxR), codons optimised for E. coli
type: SequenceAnnotation
persistent identity: annotation1904928
display id: annotation1904928
version: 1
top level: BBa_J28031
location: https://synbiohub.org/public/igem/BBa_J28031/annotation1904928/range1904928/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J28031/annotation1904928/range1904928/1
type: Range
persistent identity: range1904928
display id: range1904928
version: 1
top level: BBa_J28031
direction: direction/reverse
start: 138
end: 893
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1904929
display id: annotation1904929
version: 1
top level: BBa_J28031
location: https://synbiohub.org/public/igem/BBa_J28031/annotation1904929/range1904929/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_J28031/annotation1904929/range1904929/1
type: Range
persistent identity: range1904929
display id: range1904929
version: 1
top level: BBa_J28031
direction: direction/reverse
start: 900
end: 911
orientation: inline

BamHI restriction site
type: SequenceAnnotation
persistent identity: annotation1904930
display id: annotation1904930
version: 1
top level: BBa_J28031
location: https://synbiohub.org/public/igem/BBa_J28031/annotation1904930/range1904930/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J28031/annotation1904930/range1904930/1
type: Range
persistent identity: range1904930
display id: range1904930
version: 1
top level: BBa_J28031
direction: direction/forward
start: 913
end: 918
orientation: inline

BBa_R0011 (lac, lambda cI hybrid)
type: SequenceAnnotation
persistent identity: annotation1904931
display id: annotation1904931
version: 1
top level: BBa_J28031
location: https://synbiohub.org/public/igem/BBa_J28031/annotation1904931/range1904931/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_J28031/annotation1904931/range1904931/1
type: Range
persistent identity: range1904931
display id: range1904931
version: 1
top level: BBa_J28031
direction: direction/reverse
start: 920
end: 974
orientation: inline

BspHI restriction site
type: SequenceAnnotation
persistent identity: annotation1904932
display id: annotation1904932
version: 1
top level: BBa_J28031
location: https://synbiohub.org/public/igem/BBa_J28031/annotation1904932/range1904932/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J28031/annotation1904932/range1904932/1
type: Range
persistent identity: range1904932
display id: range1904932
version: 1
top level: BBa_J28031
direction: direction/forward
start: 976
end: 981
orientation: inline

BBa_R0062 (lux pR)
type: SequenceAnnotation
persistent identity: annotation1904933
display id: annotation1904933
version: 1
top level: BBa_J28031
location: https://synbiohub.org/public/igem/BBa_J28031/annotation1904933/range1904933/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_J28031/annotation1904933/range1904933/1
type: Range
persistent identity: range1904933
display id: range1904933
version: 1
top level: BBa_J28031
direction: direction/forward
start: 983
end: 1037
orientation: inline

SalI restriction site
type: SequenceAnnotation
persistent identity: annotation1904934
display id: annotation1904934
version: 1
top level: BBa_J28031
location: https://synbiohub.org/public/igem/BBa_J28031/annotation1904934/range1904934/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J28031/annotation1904934/range1904934/1
type: Range
persistent identity: range1904934
display id: range1904934
version: 1
top level: BBa_J28031
direction: direction/forward
start: 1039
end: 1044
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1904935
display id: annotation1904935
version: 1
top level: BBa_J28031
location: https://synbiohub.org/public/igem/BBa_J28031/annotation1904935/range1904935/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_J28031/annotation1904935/range1904935/1
type: Range
persistent identity: range1904935
display id: range1904935
version: 1
top level: BBa_J28031
direction: direction/forward
start: 1046
end: 1057
orientation: inline

BBa_C0061 (luxI), codons optimised for E. coli
type: SequenceAnnotation
persistent identity: annotation1904936
display id: annotation1904936
version: 1
top level: BBa_J28031
location: https://synbiohub.org/public/igem/BBa_J28031/annotation1904936/range1904936/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J28031/annotation1904936/range1904936/1
type: Range
persistent identity: range1904936
display id: range1904936
version: 1
top level: BBa_J28031
direction: direction/forward
start: 1064
end: 1681
orientation: inline

BBa_J69504
type: ComponentDefinition
persistent identity: BBa J69504
display id: BBa_J69504
version: 1
was generated by: iGEM to SBOL conversion
description: CI Repressible lacI Generator
created: 2007-04-23T11:00:00Z
modified: 2015-05-08T01:08:19Z
mutable provenance: Existing Reg Parts
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: lacI Repressible mCherry Reporter
discontinued: false
dominant: false
experience: experience/None
group u list: _101_
m user id: 0
owner id: 740
owning group id: 101
sample status: In stock
status: status/Available
bookmark: false
mutable notes: NA
star: false
creator: James Brown
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0015
component: BBa_E0030
component: BBa_B0034
component: BBa_R0051
sequence annotation: BBa_R0051
sequence annotation: BBa_B0034
sequence annotation: BBa_E0030
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_J69504_sequence/1

BBa_B0015
type: Component
persistent identity: component2166304
display id: component2166304
version: 1
top level: BBa_J69504
definition: BBa_B0015
access: public

BBa_E0030
type: Component
persistent identity: component2166297
display id: component2166297
version: 1
top level: BBa_J69504
definition: eyfp
access: public

BBa_B0034
type: Component
persistent identity: component2166296
display id: component2166296
version: 1
top level: BBa_J69504
definition: BBa_B0034
access: public

BBa_R0051
type: Component
persistent identity: component2166291
display id: component2166291
version: 1
top level: BBa_J69504
definition: cI lam
access: public

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation2166291
display id: annotation2166291
version: 1
top level: BBa_J69504
location: https://synbiohub.org/public/igem/BBa_J69504/annotation2166291/range2166291/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_J69504/annotation2166291/range2166291/1
type: Range
persistent identity: range2166291
display id: range2166291
version: 1
top level: BBa_J69504
direction: direction/forward
start: 1
end: 49
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2166296
display id: annotation2166296
version: 1
top level: BBa_J69504
location: https://synbiohub.org/public/igem/BBa_J69504/annotation2166296/range2166296/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J69504/annotation2166296/range2166296/1
type: Range
persistent identity: range2166296
display id: range2166296
version: 1
top level: BBa_J69504
direction: direction/reverse
start: 58
end: 69
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation2166297
display id: annotation2166297
version: 1
top level: BBa_J69504
location: https://synbiohub.org/public/igem/BBa_J69504/annotation2166297/range2166297/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_J69504/annotation2166297/range2166297/1
type: Range
persistent identity: range2166297
display id: range2166297
version: 1
top level: BBa_J69504
direction: direction/reverse
start: 76
end: 798
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2166304
display id: annotation2166304
version: 1
top level: BBa_J69504
location: https://synbiohub.org/public/igem/BBa_J69504/annotation2166304/range2166304/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_J69504/annotation2166304/range2166304/1
type: Range
persistent identity: range2166304
display id: range2166304
version: 1
top level: BBa_J69504
direction: direction/reverse
start: 807
end: 935
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

eyfp
type: ComponentDefinition
persistent identity: BBa E0030
display id: BBa_E0030
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced yellow fluorescent protein derived from A. victoria GFP
created: 2004-03-02T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modificaitons to Clontech EYFP by Reshma Shetty
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_E0030_sequence/1

cI lam
type: ComponentDefinition
persistent identity: BBa R0051
display id: BBa_R0051
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lambda cI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Incompatible with host expressing cI repressor.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0051
sequence annotation: OR2
sequence annotation: -35
sequence annotation: OR1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0051_sequence/1

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation7067
display id: annotation7067
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
type: Range
persistent identity: range7067
display id: range7067
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 49
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2025
display id: annotation2025
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
type: Range
persistent identity: range2025
display id: range2025
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 17
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2023
display id: annotation2023
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
type: Range
persistent identity: range2023
display id: range2023
version: 1
top level: cI lam
direction: direction/forward
start: 15
end: 20
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2024
display id: annotation2024
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
type: Range
persistent identity: range2024
display id: range2024
version: 1
top level: cI lam
direction: direction/forward
start: 25
end: 41
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2022
display id: annotation2022
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
type: Range
persistent identity: range2022
display id: range2022
version: 1
top level: cI lam
direction: direction/forward
start: 38
end: 43
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_J69020_sequence/1
type: Sequence
persistent identity: BBa J69020 sequence
display id: BBa_J69020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggtgcatgcgttattagtttttgaaatacgggcaatcgatcgcaccggtcaggatagctttgctgatagattgacaacgattcgtggtgttcagtttcatctgcgcgttggtcagatggaaagttacggtgcgttcagagcaacccagaattttggagatgtcccaagaggacttaccttcgcacgcccatgccaggcattctttctcgcgtttggtcaggtcgttgttgcttttgttattggcaatgttaatcttgcgatagttatcaaccagggacggaacgatcagaggaatgttcatgcacgcgtgcaggaacagagaatcgatgtagttgtctttttcggagtgcgcaaaagacagcataccgaagccgttgttcgcggtatgaatcgggaagctgaaaccggtgatcagaccgctggtttttgcttctttgataacgtttggggactttttgttaaccgcgttgttctcaaagatattccagttaatcggggaatggttagagttagagtaatctacgatcggatcgtacttgatcaggttggcgtcatcatagtactgacgccatttttttgggtagttatccagaatagaaatatcggattttaccatgctgtgcggataaatgatggccagcagataatattcacagtgaaccattttggtcatatcagacaggcattggttaatatcgttgttagaacgacacgctttgattttgttaatgatacggtaggtgtcgtccgcgttgatgtttttcatctagtatttctcctcttttggatccgtgtgctcagtatcttgttatccgctcacaatgtcaattgttatccgctcacaattttcatgagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatgtcgacgaaagaggagaaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J28031_sequence/1
type: Sequence
persistent identity: BBa J28031 sequence
display id: BBa_J28031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggtgcatgcgttattagtttttgaaatacgggcaatcgatcgcaccggtcaggatagctttgctgatagattgacaacgattcgtggtgttcagtttcatctgcgcgttggtcagatggaaagttacggtgcgttcagagcaacccagaattttggagatgtcccaagaggacttaccttcgcacgcccatgccaggcattctttctcgcgtttggtcaggtcgttgttgcttttgttattggcaatgttaatcttgcgatagttatcaaccagggacggaacgatcagaggaatgttcatgcacgcgtgcaggaacagagaatcgatgtagttgtctttttcggagtgcgcaaaagacagcataccgaagccgttgttcgcggtatgaatcgggaagctgaaaccggtgatcagaccgctggtttttgcttctttgataacgtttggggactttttgttaaccgcgttgttctcaaagatattccagttaatcggggaatggttagagttagagtaatctacgatcggatcgtacttgatcaggttggcgtcatcatagtactgacgccatttttttgggtagttatccagaatagaaatatcggattttaccatgctgtgcggataaatgatggccagcagataatattcacagtgaaccattttggtcatatcagacaggcattggttaatatcgttgttagaacgacacgctttgattttgttaatgatacggtaggtgtcgtccgcgttgatgtttttcatctagtatttctcctcttttggatccgtgtgctcagtatcttgttatccgctcacaatgtcaattgttatccgctcacaattttcatgagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatgtcgacgaaagaggagaaatactagatgaccattatgatcaagaaatctgacttcctggccatcccgtccgaagaatacaaaggcatcctgtctctgcgctaccaggttttcaaacagcgtctggaatgggacctggtggttgaaaacaacctggaatctgacgagtatgataattccaacgcagaatacatctatgcgtgtgatgatactgaaaacgtttctggttgttggcgtctgctgcctaccacgggcgattatatgctgaagtccgtatttccggagctgctgggtcagcagtccgcgccgaaagacccgaacattgtagaactgtctcgcttcgcggttggcaagaacagctctaaaatcaacaactccgcaagcgaaattaccatgaaactgttcgaagctatttataaacacgcggtttcccagggtatcaccgaatacgtgaccgtcactagcaccgctatcgagcgcttcctgaaacgtatcaaggttccgtgccaccgtatcggtgacaaagaaatccacgtactgggtgataccaagagcgtcgtgctgtccatgccgattaacgagcagtttaagaaagctgtgctgaacgccgctaacgacgaaaactacgcgctggtggcgtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J69504_sequence/1
type: Sequence
persistent identity: BBa J69504 sequence
display id: BBa_J69504_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0030_sequence/1
type: Sequence
persistent identity: BBa E0030 sequence
display id: BBa_E0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0051_sequence/1
type: Sequence
persistent identity: BBa R0051 sequence
display id: BBa_R0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z