##gff-version 3
##sequence-region BBa_J69021 1 2629
BBa_J69021	.	engineered_region	1	940	.	+	0	ID=BBa_J69524;Name=BBa_J69524
BBa_J69021	.	CDS	81	803	.	+	0	ID=BBa_E0030;Name=BBa_E0030;Parent=BBa_J69524
BBa_J69021	.	terminator	812	940	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_J69524
BBa_J69021	.	terminator	812	891	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J69021	.	engineered_region	812	891	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J69021	.	stem_loop	823	866	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J69021	.	terminator	900	940	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J69021	.	engineered_region	900	940	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J69021	.	polyA_site	927	940	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J69021	.	stop_codon	933	933	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J69021	.	stem_loop	907	926	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J69021	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J69524
BBa_J69021	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J69021	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J69021	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J69021	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J69021	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J69021	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J69524
BBa_J69021	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J69021	.	engineered_region	949	2629	.	+	0	ID=BBa_J28031;Name=BBa_J28031
BBa_J69021	.	promoter	1931	1985	.	+	0	ID=annotation1904933;Name=BBa_R0062 (lux pR);Parent=BBa_J28031
BBa_J69021	.	CDS	1086	1841	.	+	0	ID=annotation1904928;Name=BBa_C0062 (luxR), codons optimised for E. coli;Parent=BBa_J28031
BBa_J69021	.	ribosome_entry_site	1994	2005	.	+	0	ID=annotation1904935;Name=BBa_B0034;Parent=BBa_J28031
BBa_J69021	.	sequence_feature	1861	1866	.	+	0	ID=annotation1904930;Name=BamHI restriction site;Parent=BBa_J28031
BBa_J69021	.	sequence_feature	1079	1084	.	+	0	ID=annotation1904927;Name=SphI restriction site;Parent=BBa_J28031
BBa_J69021	.	sequence_feature	1987	1992	.	+	0	ID=annotation1904934;Name=SalI restriction site;Parent=BBa_J28031
BBa_J69021	.	engineered_region	949	2629	.	+	0	ID=annotation1904937;Name=BBa_J28031;Parent=BBa_J28031
BBa_J69021	.	promoter	1868	1922	.	+	0	ID=annotation1904931;Name=BBa_R0011 (lac, lambda cI hybrid);Parent=BBa_J28031
BBa_J69021	.	CDS	2012	2629	.	+	0	ID=annotation1904936;Name=BBa_C0061 (luxI), codons optimised for E. coli;Parent=BBa_J28031
BBa_J69021	.	sequence_feature	1924	1929	.	+	0	ID=annotation1904932;Name=BspHI restriction site;Parent=BBa_J28031
BBa_J69021	.	ribosome_entry_site	1848	1859	.	+	0	ID=annotation1904929;Name=BBa_B0034;Parent=BBa_J28031
BBa_J69021	.	stem_loop	949	1077	.	+	0	ID=annotation1904926;Name=BBa_B0015;Parent=BBa_J28031
>BBa_J69021
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccac
aagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggca
agctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacat
gaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggc
aactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttca
aggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagca
gaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccag
cagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaag
accccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagct
gtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagag
tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaa
acgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggtgcatgcgttattagtttttgaaatacgg
gcaatcgatcgcaccggtcaggatagctttgctgatagattgacaacgattcgtggtgttcagtttcatctgcgcgttg
gtcagatggaaagttacggtgcgttcagagcaacccagaattttggagatgtcccaagaggacttaccttcgcacgccc
atgccaggcattctttctcgcgtttggtcaggtcgttgttgcttttgttattggcaatgttaatcttgcgatagttatc
aaccagggacggaacgatcagaggaatgttcatgcacgcgtgcaggaacagagaatcgatgtagttgtctttttcggag
tgcgcaaaagacagcataccgaagccgttgttcgcggtatgaatcgggaagctgaaaccggtgatcagaccgctggttt
ttgcttctttgataacgtttggggactttttgttaaccgcgttgttctcaaagatattccagttaatcggggaatggtt
agagttagagtaatctacgatcggatcgtacttgatcaggttggcgtcatcatagtactgacgccatttttttgggtag
ttatccagaatagaaatatcggattttaccatgctgtgcggataaatgatggccagcagataatattcacagtgaacca
ttttggtcatatcagacaggcattggttaatatcgttgttagaacgacacgctttgattttgttaatgatacggtaggt
gtcgtccgcgttgatgtttttcatctagtatttctcctcttttggatccgtgtgctcagtatcttgttatccgctcaca
atgtcaattgttatccgctcacaattttcatgagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttata
gtcgaataaatgtcgacgaaagaggagaaatactagatgaccattatgatcaagaaatctgacttcctggccatcccgt
ccgaagaatacaaaggcatcctgtctctgcgctaccaggttttcaaacagcgtctggaatgggacctggtggttgaaaa
caacctggaatctgacgagtatgataattccaacgcagaatacatctatgcgtgtgatgatactgaaaacgtttctggt
tgttggcgtctgctgcctaccacgggcgattatatgctgaagtccgtatttccggagctgctgggtcagcagtccgcgc
cgaaagacccgaacattgtagaactgtctcgcttcgcggttggcaagaacagctctaaaatcaacaactccgcaagcga
aattaccatgaaactgttcgaagctatttataaacacgcggtttcccagggtatcaccgaatacgtgaccgtcactagc
accgctatcgagcgcttcctgaaacgtatcaaggttccgtgccaccgtatcggtgacaaagaaatccacgtactgggtg
ataccaagagcgtcgtgctgtccatgccgattaacgagcagtttaagaaagctgtgctgaacgccgctaacgacgaaaa
ctacgcgctggtggcgtaataa